O papel da administração e liderança do Gabinete do Ensino Superior da Região Autónoma da Madeira na valorização do ensino superior

O presente relatório evidencia duas partes do nosso trabalho; uma primeira sobre o estágio onde registamos todas as atividades desenvolvidas durante o decorrer do mesmo no Gabinete do Ensino Superior (GES) da Região Autónoma da Madeira (RAM) e na qual utilizamos a investigação-ação como metodologia recorrente, reflexiva e autocrítica. A segunda parte debruça-se sobre o estudo de investigação empírica, cujo objetivo era avaliar a importância de prosseguir os estudos para o nível de ensino superior. Neste sentido, realizámos o estágio no GES com o intuito de perceber o papel da administração e liderança deste Gabinete na valorização deste ensino, ganhando uma experiência prática de liderança educacional. Este relatório de estágio está inserido no âmbito do Mestrado em Administração Educacional (AE) da Universidade da Madeira (UMa). No decorrer do estágio, o Diretor do GES partilhou a sua liderança e exemplificou o seu quotidiano, fazendo perceber quais são os problemas mais frequentes com que tem de lidar, e qual a sua relação com os técnicos superiores, restante equipa e comunidade educativa. Através das técnicas de investigação, investigação-ação e observação utilizadas durante o estágio, percebemos o que é realmente liderar, consolidando assim toda a teoria aprendida com uma realidade prática, onde pudemos verificar que o líder “ideal” é o que combina a gestão com a liderança. Realizamos um estudo de investigação empírica de interesse para o GES onde se pretendeu saber quais são as motivações dos estudantes de 12º ano em prosseguirem os seus estudos para o ensino superior, para que desta forma fosse possível identificar o nível de motivação dos jovens, podendo assim tirar conclusões e encontrar possíveis soluções. Este estudo surgiu com base nas dúvidas e incertezas inerentes à nossa sociedade devido à conjuntura económica atual que questiona o prosseguimento ou não dos estudos para o ensino superior. A metodologia utilizada foi a qualitativa e a quantitativa, tratando-se de uma metodologia mista porque baseamo-nos na recolha de dados através de seis entrevistas realizadas a diretoras de turma de (9º e 12º ano) e 58 questionários implementados, bem como de pesquisas bibliográficas, documentais e legislativas. Concluímos que o Diretor do GES é um líder democrático pois toma a atitude de projetar e concretizar as atividades que têm de ser desenvolvidas, tendo sempre em atenção problemas e necessidades que surgem no grupo. Relativamente ao estudo de investigação empírica algumas das soluções encontradas para que a motivação de prosseguir os estudos continue e aumente passa por criar perspetivas de futuro mais positivas, haver mais oferta de cursos na nossa Universidade da Madeira e também mais emprego. Trabalhar esta motivação depende sobretudo das forças políticas, das famílias e dos professores. Assim, entendemos que os professores são líderes porque têm o papel de motivar os estudantes. Concluímos que a maioria dos estudantes do ensino secundário inquiridos estão motivados em ingressar no ensino superior, sobretudo pela motivação de obterem melhores condições de vida. Os que não se encontram motivados justificam que não o estão sobretudo pela atual condição do país. Todas as entrevistadas e a maioria dos inquiridos concordam que, e tal como a nossa revisão de literatura revela, estudar no ensino superior ainda compensa

Passive discomfort index as an alternative to predicted mean vote and predicted percentage of dissatisfied to assess occupant’s thermal discomfort in dwellings

Besides energy poverty, a certain tolerance to discomfort justifies Portugal's low heating energy consumption. Once Portuguese buildings stock, previous to 1990, has a weak energy performance, it is crucial to reflect on how to make renovations with more assertive benefits (besides energy), such as those related to the health and comfort of occupants. Hence, we have studied a single house in the TRNSYS dynamic simulation tool. We performed a sensitivity analysis by simulating the same building in three locations based on Portuguese climatic winter zones: I1 – Santarém, I2 – Santa Maria da Feira, and I3 – Guarda, for two ranges of wall insulation thickness, in free float. Considering Fanger's Predicted Mean Vote (PMV) and Predicted Percentage of Dissatisfied (PPD) as the Passive Discomfort Index IDP, results suggest that lowering 20 mm on the insulation thickness does not substantially impact occupants' discomfort in winter. As the variations between scenarios considering PPD > 15%, PMV < -1, or IDP (Tair < 18 ºC) are very similar, IDP could be an alternative to PMV/PPD for assessing thermal discomfort in dwellings.publishe

IgG4- related disease: an orphan disease with many faces

Immunoglobulin G4- related disease (IgG4-RD) is a rare systemic fibro-inflammatory disorder (ORPHA284264). Although patients have been described more than 100 years ago, the systemic nature of this disease has been recognized in the 21(st) century only. Type 1 autoimmune pancreatitis is the most frequent manifestation of IgG4-RD. However, IgG4-RD can affect any organ such as salivary glands, orbits, retroperitoneum and many others. Recent research enabled a clear clinical and histopathological description of IgG4-RD. Typically, lymphoplasmacellular inflammation, storiform fibrosis and obliterative phlebitis are found in IgG4-RD biopsies and the tissue invading plasma cells largely produce IgG4. Elevated serum IgG4 levels are found in many but not all patients. Consequently, diagnostic criteria for IgG4-RD have been proposed recently. Treatment is largely based on clinical experience and retrospective case series. Glucocorticoids are the mainstay of therapy, although adjunctive immunosuppressive agents are used in relapsing patients. This review summarizes current knowledge on clinical manifestations, pathophysiology and treatment of IgG4-RD

Residential building rehabilitation in Porto historic center: case study analysis by using a simulation model

Nowadays, rehabilitation in historic centers can become a challenge due to the restrictions concerning the requirements for the building envelope. Portugal has conducted studies to define the maximum U values [W/(m2 K)] for single building components based on the indoor temperature. Despite the relative humidity being crucial for thermal comfort, the current Portuguese scheme for energy certification of buildings does not consider it. Additionally, it is essential to know that most of the dwellings in Portugal are in a fleet-float regime due to cultural habits and energy poverty. Therefore, increasing the insulation thickness of exterior walls might not have a relevant impact on the indoor temperature. There is even the risk of harming the authenticity of the built heritage. Using a calibrated dynamic simulation model developed with TRNSYS software, we found that, in winter, the effect of external wall insulation on the indoor temperature is tiny. Results suggest that maximum U values [W/(m2 K)] for exterior walls might be over-defined. Further studies of cost-optimal levels should be conducted with calibrated models in a free-float regime to define the maximum U values [W/(m2 K)] of external walls for this type of buildings.publishe

Mutagenesis of Murine Cytomegalovirus Using a Tn3-Based Transposon

AbstractA transposon derived from Escherichia coli Tn3 was introduced into the genome of murine cytomegalovirus (MCMV) to generate a pool of viral mutants. We analyzed three of the constructed recombinant viruses that contained the transposon within the M25, M27, and m155 open reading frames. Our studies provide the first direct evidence to suggest that M25 and M27 are not essential for viral replication in mouse NIH 3T3 cells. Studies in cultured cells and Balb/c mice indicated that the transposon insertion is stable during viral propagation both in vitro and in vivo. Moreover the virus that contained the insertion mutation in M25 exhibited a titer similar to that of the wild-type virus in the salivary glands, lungs, livers, spleens, and kidneys of the Balb/c mice that were intraperitoneally infected with these viruses. These results suggest that M25 is dispensable for viral growth in these organs and the presence of the transposon sequence in the viral genome does not significantly affect viral replication in vivo. The Tn3-based system can be used as a mutagenesis approach for studying the function of MCMV genes in both tissue culture and in animals

RESTORE Survey on the Public Perception of Advanced Therapies and ATMPs in Europe — Why the European Union Should Invest More!

Advanced therapy medicinal products (ATMPs) are potential game changers in modern medical care with an anticipated major impact for patients and society. They are a new drug class often referred to as "living drugs," and are based on complex components such as vectors, cells and even tissues. The production of such ATMPs involves innovative biotechnological methods. In this survey, we have assessed the perception of European citizens regarding ATMPs and health care in Europe, in relation to other important topics, such as safety and security, data protection, climate friendly energy supply, migration, and others. A crucial question was to determine to what extent European citizens wish to support public funding of innovations in healthcare and reimbursement strategies for ATMPs. To answer this, we conducted an online survey in 13 European countries (representative of 85.3% of the entire EU population including the UK in 2020), surveying a total of 7,062 European citizens. The survey was representative with respect to adult age groups and gender in each country. Healthcare had the highest ranking among important societal topics. We found that 83% of the surveyed EU citizens were in support of more public funding of technologies in the field of ATMPs. Interestingly, 74% of respondents are in support of cross-border healthcare for patients with rare diseases to receive ATMP treatments and 61% support the reimbursement of very expensive ATMPs within the European health care system despite the current lack of long-term efficacy data. In conclusion, healthcare is a top ranking issue for European Citizens, who additionally support funding of new technologies to enable the wider application of ATMPs in Europe

Solid-phase-assisted synthesis of targeting peptide-PEG-oligo(ethane amino)amides for receptor-mediated gene delivery.

In the forthcoming era of cancer gene therapy, efforts will be devoted to the development of new efficient and non-toxic gene delivery vectors. In this regard, the use of Fmoc/Boc-protected oligo(ethane amino)acids as building blocks for solid-phase-supported assembly represents a novel promising approach towards fully controlled syntheses of effective gene vectors. Here we report on the synthesis of defined polymers containing the following: (i) a plasmid DNA (pDNA) binding domain of eight succinoyl-tetraethylenpentamine (Stp) units and two terminal cysteine residues; (ii) a central polyethylene glycol (PEG) chain (with twenty-four oxyethylene units) for shielding; and (iii) specific peptides for targeting towards cancer cells. Peptides B6 and c(RGDfK), which bind transferrin receptor and αvβ3 integrin, respectively, were chosen because of the high expression of these receptors in many tumoral cells. This study shows the feasibility of designing these kinds of fully controlled vectors and their success for targeted pDNA-based gene transfer

Mixtures of Estrogenic Chemicals Enhance Vitellogenic Response in Sea Bass

BACKGROUND: The potential impact of natural and synthetic estrogens on aquatic ecosystems has attracted considerable attention because it is currently accepted that their joint effects are more severe when they are present in mixtures. Although it is well-known that they occur as mixtures in the marine environment, there is little information about the combined effects of estrogenic chemicals on marine biota. OBJECTIVE: In 14-day tests with juvenile sea bass, we analyzed singly and in combination the estrogenic activity of estradiol (E2), ethynylestradiol (EE2), and bisphenol A (BPA) using vitellogenin induction as an end point. METHODS: Fish were exposed to each compound, and on the basis of these concentration-response data, we predicted mixture effects by applying the model of concentration addition. The mixtures were tested using a fixed-ratio design, and the resulting mixture effects were compared to the predictions. RESULTS: EE2 was the most potent steroid, with an EC50 (median effective concentration) of 0.029 μg/L, 3.6 times more potent than E2 (EC50 = 0.104 μg/L); BPA was the least potent chemical, with an EC50 of 77.94 μg/L. The comparative assessment yielded a good agreement between observed and predicted mixture effects. CONCLUSIONS: This study demonstrates the potential hazard of these compounds to seawater life by their ability to act together in an additive manner. It provides evidence that concentration addition can be used as a predictive tool for assessing the combined effects of estrogenic chemicals in marine ecosystems

Application of qPCR for multicopper oxidase gene (MCO) in biogenic amines degradation by Lactobacillus casei

Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we designed two sets of primers for the detection and quantification of the multicopper oxidase gene (MCO), which encodes an enzyme involved in BAs degradation, and endogenous (glyceraldehyde-3-phosphate dehydrogenase) gene (GAPDH) in Lactobacillus casei group by real-time PCR (qPCR). We tested 15 Lactobacillus strains in the screening assays (thus, MCO gene possessing assay (PCR) and monitoring of BAs degradation by HPLC-UV), in which Lactobacillus casei CCDM 198 exhibited the best degradation abilities. For this strain, we monitored the expression of the target gene (MCO) in time (qPCR), the effect of redox treatments (cysteine, ascorbic acid) on the expression of the gene, and the ability to degrade BAs not only in a modified MRS medium (MRS/2) but also in a real food sample (milk). Moreover, decarboxylase activity (ability to form BAs) of this strain was excluded. According to the results, CCDM 198 significantly (P < 0.05) reduced BAs (putrescine, histamine, tyramine, cadaverine), up to 25% decline in 48 h. The highest level of relative expression of MCO (5.21 ± 0.14) was achieved in MRS/2 media with cysteine. © 2020 Elsevier LtdGrant Agency of the Czech Republic (GACR)Grant Agency of the Czech Republic [17.09594S]; Internal Grant of TBU in Zlin [IGA/FT/2020/009