Effect of ELISA kit manufacturing process and incubation time on progesterone concentration measured in dog serum for ovulation diagnosis – Short communication

Twenty-two serum samples of healthy bitches were tested with the frozen and lyophilised version of the same ELISA kit (Quanticheck, Faculty of Veterinary Science, Budapest, Hungary). Samples were chosen on the basis of their progesterone (P4) concentrations, which were between 1.00 and 20.00 ng/mL. As it is well known, this range has the highest clinical relevance in ovulation diagnosis. Both types of microplates were read at 15-min intervals from the 15th until the 90th minute (min) of incubation, and the results were compared with those of frozen plates at 60 min of incubation as 100 percent. Lyophilised microplates gave on average 18 percent higher results than the frozen version at equal incubation times. The highest difference between lyophilised and frozen samples was observed at 45 and 60 min of incubation. Ninety-four percent of the reaction in the frozen microplate occurred in the first 15 min, and during the subsequent 30 min the reaction seemingly stopped. After the 45th min of incubation, this 94 percent increased to 108 percent in the subsequent 30 min, which remained the final approximate result at the end of the 90 min of incubation. In contrast to the frozen microplate, the measured concentration increased continuously in the lyophilised version and reached the highest level at the 60th min. The results of the lyophilised microplate reached the same level at 30 min of incubation as those of the frozen version at 60 min. In conclusion, a mechanical increase or decrease of the incubation time does not generate a linear change in the test results. This study demonstrated that the results of a series of samples collected from the same bitch cannot be compared if they are measured with different laboratory methods or different ELISA kits

Comparison of measures of crowding, group size, and diversity

In ecology, diversity is often measured as the mean rarity of species in a community. In behavioral sciences and parasitology, mean crowding is the size of the group to which a typical individual belongs. In this paper, focusing mostly on the mathematical aspect, we demonstrate that diversity and crowding are closely related notions. We show that mean crowding can be transformed into diversity and vice versa. Based on this general equivalence rule, notions, relationships, and methods developed in one field can be adapted to the other one. In relation to crowding,we introduce the notion “effective number of groups” that corresponds to the “effective number of species” used in diversity studies.We define new aggregation indices thatmirror evenness indices known from diversity theory. We also construct aggregation profiles and orderings of populations based on aggregation indices. By uniting the mathematical interpretation of the ecological notion of diversity and the ethological notion of typical group size (or crowding, in parasitology), our insight opens a new avenue of both theoretical andmethodological research. This is exemplified here using real-life abundance data of avian parasites

A klímaváltozás közösségökológiai hatásainak elemzései

A klímaváltozás ökológiai hatásainak elemzésével kapcsolatos tudományos eredményeink megközelítési módjukat tekintve az alábbi hat fő csoportba oszthatók: 1. Az elméleti várakozások tisztázása stratégiai modellezéssel. 2. Lehetséges hatások mértékének behatárolása Magyarország vonatkozásában, földrajzi analógiai megközelítéssel. 3. Nagy monitoring adatbázisok elemzései a már bekövetkezett változási tendenciák feltárására. 4. Természetközeli populációk és ökoszisztémák várható változásainak elemzése taktikai modellezéssel és statisztikai elemzésekkel. 5. A klímaváltozás agroökoszisztémákra gyakorolt hatásai 6. Új, hatékony és a korábbiaknál általánosabban használható bioindikátor-rendszer kidolgozása. A továbbiakban eredményeinket ezen felosztás szerinti csoportosításban ismertetjük

Effect of low-dose zearalenone exposure on luteal function, follicular activity and uterine oedema in cycling mares

The effect of 10-day zearalenone administration starting 10 days after ovulation was studied in 6 cycling trotter mares in the summer period. After an entire oestrous cycle (Cycle 1), mares were given 7 mg purified zearalenone per os daily (1 mg/ml in ethyl alcohol) beginning on Day 10 of Cycle 2. Toxin exposure was continued until the subsequent ovulation. Luteal function and follicular activity were monitored daily by rectal palpation, ultrasonography and blood sampling for progesterone. During toxin exposure, all animals were in good physical condition. The toxin had no effect on the length of the interovulatory intervals, luteal and follicular phases. It did not influence significantly the plasma progesterone profiles (logistic curve parameters A1 to A6), the follicular activity (growth rate, maximum size of the ovulatory follicles, maximum number and the time of first increase in the number of large follicles) and the uterine oedema. It is concluded that in cyclic mares the methods used in this study could not detect any adverse effect of zearalenone (administered at a low dose similar to natural exposure) on reproduction

Seeking systematicity in variation : theoretical and methodological considerations on the “variety” concept

One centennial discussion in linguistics concerns whether languages, or linguistic systems, are, essentially, homogeneous or rather show “structured heterogeneity.” In this contribution, the question is addressed whether and how sociolinguistically defined systems (or ‘varieties’) are to be distinguished in a heterogeneous linguistic landscape: to what extent can structure be found in the myriads of language variants heard in everyday language use? We first elaborate on the theoretical importance of this ‘variety question’ by relating it to current approaches from, among others, generative linguistics (competing grammars), sociolinguistics (style-shifting, polylanguaging), and cognitive linguistics (prototype theory). Possible criteria for defining and detecting varieties are introduced, which are subsequently tested empirically, using a self-compiled corpus of spoken Dutch in West Flanders (Belgium). This empirical study demonstrates that the speech repertoire of the studied West Flemish speakers consists of four varieties, viz. a fairly stable dialect variety, a more or less virtual standard Dutch variety, and two intermediate varieties, which we will label ‘cleaned-up dialect’ and ‘substandard.’ On the methodological level, this case-study underscores the importance of speech corpora comprising both inter- and intra-speaker variation on the one hand, and the merits of triangulating qualitative and quantitative approaches on the other

Moving Cages Further Offshore: Effects on Southern Bluefin Tuna, T. maccoyii, Parasites, Health and Performance

The effects of offshore aquaculture on SBT health (particularly parasitic infections and haematology) and performance were the main aim of this study. Two cohorts of ranched Southern Bluefin tuna (SBT) (Thunnus maccoyii) were monitored throughout the commercial season, one maintained in the traditional near shore tuna farming zone and one maintained further offshore. SBT maintained offshore had reduced mortality, increased condition index at week 6 post transfer, reduced blood fluke and sealice loads, and haematological variables such as haemoglobin or lysozyme equal to or exceeding near shore maintained fish. The offshore cohort had no Cardicola forsteri and a 5% prevalence of Caligus spp., compared to a prevalence of 85% for Cardicola forsteri and 55% prevalence for Caligus spp. near shore at 6 weeks post transfer. This study is the first of its kind to examine the effects of commercial offshore sites on farmed fish parasites, health and performance