Smaaklessen beproefd: een formatieve procesevaluatie

Smaaklessen is een lesprogramma voor leerlingen in het primair onderwijs waar scholen zich sinds schooljaar 2006-2007 gratis voor kunnen inschrijven. In dit onderzoek is met een formatieve procesevaluatie nagegaan in hoeverre het huidige lespakket wordt gebruikt en gewaardeerd. Daarnaast is gekeken hoe de organisatie van Smaaklessen is en wordt gewaardeerd. In het onderzoek staan drie niveaus centraal: scholen, regio’s (HASsen en GGD'en) en de nationale coördinatie (WUR). Op alle niveaus zijn data verzameld via observaties (8 scholen, 19 lessen), vragenlijsten (18 leerkrachten en 39 leerlingen) en via gesprekken met leerlingen, leerkrachten, schoolleiders, betrokkenen bij HASsen, GGD’en en de WUR

Factsheets SYSTEMIC Outreach Location

GreenGas AD Plant is a farm based anaerobic digestion plant located near Shanagolden in County Limerick, Ireland. The the 250 kW el plant was constructed in the course of 2010 and when commissioned in 2011 it was one of the first such plants in Ireland. The plant has undergone a number of upgrades and now operates at 1MW e

Factsheets SYSTEMIC Demonstration Plant

Groot Zevert Vergisting (GZV) is located in Beltrum, in the eastern province of Gelderland (the Netherlands). The Demonstration plant operates from 2004 and employs 40 full time equivalent (Table 1). It is a fast-growing company whose treatment capacity is 102 kt per year, making it one of the largest anaerobic digestion (AD) plant in the Netherlands. GZV has the ambition to become the first “Green Mineral Mining” Centre in the Netherlands

Verkenning herstel kleinschalige lijnvormige infrastructuur Heuvelland

Kalkgraslanden en hellingbossen zijn voorbeelden van soortenrijke natuur in het Limburgse Heuvelland. In het kader van OBN is een verkennend overzicht van natuurwaarden van lijnvormige elementen gemaakt, met een visie op mogelijk herstel van natuur in bermen, heggen en houtwallen. Deze inventarisatie is tot stand gekomen dankzij de inzet van: RAVON, Vlinderstichting, Stichting Anemoon, Floron, Zoogdierenvereniging VZZ, EIS Nederland, en Alterr

Solid fraction of separated digestate as soil improver : implications for soil fertility and carbon sequestration

Purpose This study investigated the C and N mineralisation potential of solid fractions (SFs) from co-digestated pig manure after P-stripping (P-POOR SF) in comparison with P-rich SFs, as a means to estimate their organic matter stability in soil. Compost (COMP) and biochar (BCHR) (made from P-POOR SF) were also included in the study as reference biosolids. Methods The SFs were incubated in a sandy-loam soil under moist conditions to determine production of CO(2)and mineral N. At specified intervals, CO(2)evolution in the mixtures was measured via the alkali trap method and titration over a period of 81 days, while mineral N was measured using a flow analyser after KCl extraction over a period of 112 days. Results The various SFs showed similar patterns of C mineralisation (15-26% of added total C in 81 days) that were clearly higher than for COMP and BCHR (6% and 7%, respectively). Temporary N immobilisation was observed in biosolids with a high C/N ratio. The effective organic matter (EOM) of the SFs was calculated based on the C mineralisation data and varied between 130 and 369 kg Mg-1. Conclusions The SF with a reduced P content had a high EOM/P ratio which is beneficial in areas where P status of the soil is already high. Moreover, the N mineralisation patterns confirm that a high C/N ratio may also reduce risks for N leaching due to temporary N immobilisation

Fosfaatvormen in melkveemest en potentieel voor terugwinning

This report describes the results of the research into the phosphate forms and the potential of recovery of phosphate from dairy manure. On different dairy farms with different types of manure separators, slurry robots and scrapers samples were taken from the manure, liquid fraction, thick fraction and/or faeces and analyzed on the content of different phosphate forms. Furthermore calcium hydroxide was added to the liquid fraction to separate the orthophosphate with the sediment and are the thick fractions diluted and acidified to pH 6 and 5 to determine the amount of phosphate being released as orthophosphate. On the basis of these analysis is the potential for recovery of phosphate determined

Lerarenhandleing groep 7 : lesprogramma over eten en smaak voor het basisonderwijs

Het programma Smaaklessen is bedoeld voor leerkrachten van het basisonderwijs en hun leerlingen van 4-12 jaar. De leskaternen zijn bedoeld als handleiding voor de leerkracht en bevatten allerlei lessuggesties. Deze docentenhandleiding is bedoeld voor groep 7. Smaaklessen heeft als doel kinderen gevoelig te maken voor een nieuwe beleving van voedsel

Inleidend katern groep 1 t/m 8 : lesprogramma over eten en smaak voor het basisonderwijs

Het programma Smaaklessen is bedoeld voor leerkrachten van het basisonderwijs en hun leerlingen van 4-12 jaar. De leskaternen zijn bedoeld als handleiding voor de leerkracht en bevatten allerlei lessuggesties. Dit inleidend katern is bedoeld voor de groepen 1 t/m 8. Smaaklessen heeft als doel kinderen gevoelig te maken voor een nieuwe beleving van voedsel

Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers

"This is the peer reviewed version of the following article: Gottselig, N., W. Amelung, J. W. Kirchner, R. Bol, W. Eugster, S. J. Granger, C. Hernández-Crespo, et al. 2017. Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers. Global Biogeochemical Cycles 31 (10). American Geophysical Union (AGU): 1592 1607. doi:10.1002/2017gb005657, which has been published in final form at https://doi.org/10.1002/2017GB005657. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."[EN] Biogeochemical cycling of elements largely occurs in dissolved state, but many elements may also be bound to natural nanoparticles (NNP, 1-100 nm) and fine colloids (100-450 nm). We examined the hypothesis that the size and composition of stream water NNP and colloids vary systematically across Europe. To test this hypothesis, 96 stream water samples were simultaneously collected in 26 forested headwater catchments along two transects across Europe. Three size fractions (similar to 1-20 nm, >20-60 nm, and >60 nm) of NNP and fine colloids were identified with Field Flow Fractionation coupled to inductively coupled plasma mass spectrometry and an organic carbon detector. The results showed that NNP and fine colloids constituted between 2 +/- 5% (Si) and 53 +/- 21% (Fe; mean +/- SD) of total element concentrations, indicating a substantial contribution of particles to element transport in these European streams, especially for P and Fe. The particulate contents of Fe, Al, and organic C were correlated to their total element concentrations, but those of particulate Si, Mn, P, and Ca were not. The fine colloidal fractions >60 nm were dominated by clay minerals across all sites. The resulting element patterns of NNP <60 nm changed from North to South Europe from Fe-to Ca-dominated particles, along with associated changes in acidity, forest type, and dominant lithology.The authors gratefully acknowledge the assistance of the following people in locating suitable sampling sites, contacting site operators, performing the sampling, and providing data: A. Avila Castells (Autonomous University of Barcelona), R. Batalla (University of Lleida), P. Blomkvist (Swedish University of Agricultural Sciences), H. Bogena (Julich Research Center), A.K. Boulet (University of Aveiro), D. Estany (University of Lleida), F. Garnier (French National Institute of Agricultural Research), H.J. Hendricks-Franssen (Research Center Julich), L. JacksonBlake (James Hutton Institute, NIVA), T. Laurila (Finnish Meteorological Institute), A. Lindroth (Lund University), M.M. Monerris (Universitat Politecnica de Valencia), M. Ottosson Lofvenius (Swedish University of Agricultural Sciences), I. Taberman (Swedish University of Agricultural Sciences), F. Wendland (Research Center Julich), T. Zetterberg (Swedish University of Agricultural Sciences and The Swedish Environmental Research Institute, IVL) and further unnamed contributors. The Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Integrated Monitoring, the latter financed by the Swedish Environmental Protection Agency, and ICOS Sweden have supported sampling and provided data for the Swedish sites. J.J.K. gratefully acknowledges the support from CESAM (UID/AMB/50017/2013), funded by the FCT/MCTES (PIDDAC) with cofunding by FEDER through COMPETE. N.G. gratefully acknowledges all those who contributed to organizing and implementing the continental sampling. The raw data can be found at http://hdl.handle.net/2128/14937. This project was partly funded by the German Research Foundation (DFG KL2495/1-1).Gottselig, N.; Amelung, W.; Kirchner, J.; Bol, R.; Eugster, W.; Granger, S.; Hernández Crespo, C.... (2017). Elemental Composition of Natural Nanoparticles and Fine Colloids in European Forest Stream Waters and Their Role as Phosphorus Carriers. Global Biogeochemical Cycles. 31(10):1592-1607. https://doi.org/10.1002/2017GB005657S159216073110Baken, S., Moens, C., van der Grift, B., & Smolders, E. (2016). Phosphate binding by natural iron-rich colloids in streams. Water Research, 98, 326-333. doi:10.1016/j.watres.2016.04.032Baken, S., Regelink, I. C., Comans, R. N. J., Smolders, E., & Koopmans, G. F. (2016). Iron-rich colloids as carriers of phosphorus in streams: A field-flow fractionation study. Water Research, 99, 83-90. doi:10.1016/j.watres.2016.04.060Benedetti, M. F., Van Riemsdijk, W. H., Koopal, L. K., Kinniburgh, D. G., Gooddy, D. C., & Milne, C. J. (1996). Metal ion binding by natural organic matter: From the model to the field. Geochimica et Cosmochimica Acta, 60(14), 2503-2513. doi:10.1016/0016-7037(96)00113-5Binkley, D., Ice, G. G., Kaye, J., & Williams, C. A. (2004). NITROGEN AND PHOSPHORUS CONCENTRATIONS IN FOREST STREAMS OF THE UNITED STATES. Journal of the American Water Resources Association, 40(5), 1277-1291. doi:10.1111/j.1752-1688.2004.tb01586.xBishop, K., Buffam, I., Erlandsson, M., Fölster, J., Laudon, H., Seibert, J., & Temnerud, J. (2008). Aqua Incognita: the unknown headwaters. Hydrological Processes, 22(8), 1239-1242. doi:10.1002/hyp.7049Bol, R., Julich, D., Brödlin, D., Siemens, J., Kaiser, K., Dippold, M. A., … Hagedorn, F. (2016). Dissolved and colloidal phosphorus fluxes in forest ecosystems-an almost blind spot in ecosystem research. Journal of Plant Nutrition and Soil Science, 179(4), 425-438. doi:10.1002/jpln.201600079Buffle, J., & Leppard, G. G. (1995). Characterization of Aquatic Colloids and Macromolecules. 2. Key Role of Physical Structures on Analytical Results. Environmental Science & Technology, 29(9), 2176-2184. doi:10.1021/es00009a005Celi, L., & Barberis, E. (s. f.). Abiotic stabilization of organic phosphorus in the environment. Organic phosphorus in the environment, 113-132. doi:10.1079/9780851998220.0113Dahlqvist, R., Benedetti, M. F., Andersson, K., Turner, D., Larsson, T., Stolpe, B., & Ingri, J. (2004). Association of calcium with colloidal particles and speciation of calcium in the Kalix and Amazon rivers. Geochimica et Cosmochimica Acta, 68(20), 4059-4075. doi:10.1016/j.gca.2004.04.007Darch, T., Blackwell, M. S. A., Hawkins, J. M. B., Haygarth, P. M., & Chadwick, D. (2014). A Meta-Analysis of Organic and Inorganic Phosphorus in Organic Fertilizers, Soils, and Water: Implications for Water Quality. Critical Reviews in Environmental Science and Technology, 44(19), 2172-2202. doi:10.1080/10643389.2013.790752Dynesius, M., & Nilsson, C. (1994). Fragmentation and Flow Regulation of River Systems in the Northern Third of the World. Science, 266(5186), 753-762. doi:10.1126/science.266.5186.753Erickson, H. P. (2009). Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy. Biological Procedures Online, 11(1), 32-51. doi:10.1007/s12575-009-9008-xEspinosa, M., Turner, B. L., & Haygarth, P. M. (1999). Preconcentration and Separation of Trace Phosphorus Compounds in Soil Leachate. Journal of Environmental Quality, 28(5), 1497-1504. doi:10.2134/jeq1999.00472425002800050015xFernández-Martínez, M., Vicca, S., Janssens, I. A., Sardans, J., Luyssaert, S., Campioli, M., … Peñuelas, J. (2014). Nutrient availability as the key regulator of global forest carbon balance. Nature Climate Change, 4(6), 471-476. doi:10.1038/nclimate2177Giddings, J., Yang, F., & Myers, M. (1976). Flow-field-flow fractionation: a versatile new separation method. Science, 193(4259), 1244-1245. doi:10.1126/science.959835Gimbert, L. J., Andrew, K. N., Haygarth, P. M., & Worsfold, P. J. (2003). Environmental applications of flow field-flow fractionation (FIFFF). TrAC Trends in Analytical Chemistry, 22(9), 615-633. doi:10.1016/s0165-9936(03)01103-8Gottselig, N., Bol, R., Nischwitz, V., Vereecken, H., Amelung, W., & Klumpp, E. (2014). Distribution of Phosphorus-Containing Fine Colloids and Nanoparticles in Stream Water of a Forest Catchment. Vadose Zone Journal, 13(7), vzj2014.01.0005. doi:10.2136/vzj2014.01.0005Gottselig, N., Nischwitz, V., Meyn, T., Amelung, W., Bol, R., Halle, C., … Klumpp, E. (2017). Phosphorus Binding to Nanoparticles and Colloids in Forest Stream Waters. Vadose Zone Journal, 16(3), vzj2016.07.0064. doi:10.2136/vzj2016.07.0064Hagedorn , A. G. 2006 EG-Sicherheitsdatenblatt (Gemäß 2001/58/EG)Hart, B. T., Douglas, G. B., Beckett, R., Van Put, A., & Van Grieken, R. E. (1993). Characterization of colloidal and particulate matter transported by the magela creek system, Northern Australia. Hydrological Processes, 7(1), 105-118. doi:10.1002/hyp.3360070111Hassellöv, M., Lyvén, B., Haraldsson, C., & Sirinawin, W. (1999). Determination of Continuous Size and Trace Element Distribution of Colloidal Material in Natural Water by On-Line Coupling of Flow Field-Flow Fractionation with ICPMS. Analytical Chemistry, 71(16), 3497-3502. doi:10.1021/ac981455yHassellov, M., & von der Kammer, F. (2008). Iron Oxides as Geochemical Nanovectors for Metal Transport in Soil-River Systems. Elements, 4(6), 401-406. doi:10.2113/gselements.4.6.401Hens, M., & Merckx, R. (2001). Functional Characterization of Colloidal Phosphorus Species in the Soil Solution of Sandy Soils. Environmental Science & Technology, 35(3), 493-500. doi:10.1021/es0013576Hill, D. M., & Aplin, A. C. (2001). Role of colloids and fine particles in the transport of metals in rivers draining carbonate and silicate terrains. Limnology and Oceanography, 46(2), 331-344. doi:10.4319/lo.2001.46.2.0331Jarvie, H. P., Neal, C., Rowland, A. P., Neal, M., Morris, P. N., Lead, J. R., … Hockenhull, K. (2012). Role of riverine colloids in macronutrient and metal partitioning and transport, along an upland–lowland land-use continuum, under low-flow conditions. Science of The Total Environment, 434, 171-185. doi:10.1016/j.scitotenv.2011.11.061Jiang, X., Bol, R., Nischwitz, V., Siebers, N., Willbold, S., Vereecken, H., … Klumpp, E. (2015). Phosphorus Containing Water Dispersible Nanoparticles in Arable Soil. Journal of Environmental Quality, 44(6), 1772-1781. doi:10.2134/jeq2015.02.0085Kögel-Knabner, I., & Amelung, W. (2014). Dynamics, Chemistry, and Preservation of Organic Matter in Soils. Treatise on Geochemistry, 157-215. doi:10.1016/b978-0-08-095975-7.01012-3Krám, P., Hruška, J., & Shanley, J. B. (2012). Streamwater chemistry in three contrasting monolithologic Czech catchments. Applied Geochemistry, 27(9), 1854-1863. doi:10.1016/j.apgeochem.2012.02.020Lyvén, B., Hassellöv, M., Turner, D. R., Haraldsson, C., & Andersson, K. (2003). Competition between iron- and carbon-based colloidal carriers for trace metals in a freshwater assessed using flow field-flow fractionation coupled to ICPMS. Geochimica et Cosmochimica Acta, 67(20), 3791-3802. doi:10.1016/s0016-7037(03)00087-5Marschner, B., & Kalbitz, K. (2003). Controls of bioavailability and biodegradability of dissolved organic matter in soils. Geoderma, 113(3-4), 211-235. doi:10.1016/s0016-7061(02)00362-2Martin, J.-M., Dai, M.-H., & Cauwet, G. (1995). Significance of colloids in the biogeochemical cycling of organic carbon and trace metals in the Venice Lagoon (Italy). Limnology and Oceanography, 40(1), 119-131. doi:10.4319/lo.1995.40.1.0119Mattsson, T., Kortelainen, P., Laubel, A., Evans, D., Pujo-Pay, M., Räike, A., & Conan, P. (2009). Export of dissolved organic matter in relation to land use along a European climatic gradient. Science of The Total Environment, 407(6), 1967-1976. doi:10.1016/j.scitotenv.2008.11.014Missong, A., Bol, R., Willbold, S., Siemens, J., & Klumpp, E. (2016). Phosphorus forms in forest soil colloids as revealed by liquid-state31P-NMR. Journal of Plant Nutrition and Soil Science, 179(2), 159-167. doi:10.1002/jpln.201500119Montalvo, D., Degryse, F., & McLaughlin, M. J. (2015). Natural Colloidal P and Its Contribution to Plant P Uptake. Environmental Science & Technology, 49(6), 3427-3434. doi:10.1021/es504643fNeubauer, E., Köhler, S. J., von der Kammer, F., Laudon, H., & Hofmann, T. (2013). Effect of pH and Stream Order on Iron and Arsenic Speciation in Boreal Catchments. Environmental Science & Technology, 47(13), 7120-7128. doi:10.1021/es401193jNeubauer, E., v.d. Kammer, F., & Hofmann, T. (2011). Influence of carrier solution ionic strength and injected sample load on retention and recovery of natural nanoparticles using Flow Field-Flow Fractionation. Journal of Chromatography A, 1218(38), 6763-6773. doi:10.1016/j.chroma.2011.07.010Nischwitz, V., & Goenaga-Infante, H. (2012). Improved sample preparation and quality control for the characterisation of titanium dioxide nanoparticles in sunscreens using flow field flow fractionation on-line with inductively coupled plasma mass spectrometry. Journal of Analytical Atomic Spectrometry, 27(7), 1084. doi:10.1039/c2ja10387gRan, Y., Fu, J. ., Sheng, G. ., Beckett, R., & Hart, B. . (2000). Fractionation and composition of colloidal and suspended particulate materials in rivers. Chemosphere, 41(1-2), 33-43. doi:10.1016/s0045-6535(99)00387-2Regelink, I. C., Koopmans, G. F., van der Salm, C., Weng, L., & van Riemsdijk, W. H. (2013). Characterization of Colloidal Phosphorus Species in Drainage Waters from a Clay Soil Using Asymmetric Flow Field-Flow Fractionation. Journal of Environmental Quality, 42(2), 464-473. doi:10.2134/jeq2012.0322Regelink, I. C., Voegelin, A., Weng, L., Koopmans, G. F., & Comans, R. N. J. (2014). Characterization of Colloidal Fe from Soils Using Field-Flow Fractionation and Fe K-Edge X-ray Absorption Spectroscopy. Environmental Science & Technology, 48(8), 4307-4316. doi:10.1021/es405330xRegelink, I. C., Weng, L., & van Riemsdijk, W. H. (2011). The contribution of organic and mineral colloidal nanoparticles to element transport in a podzol soil. Applied Geochemistry, 26, S241-S244. doi:10.1016/j.apgeochem.2011.03.114RICHARDSON, C. J. (1985). Mechanisms Controlling Phosphorus Retention Capacity in Freshwater Wetlands. Science, 228(4706), 1424-1427. doi:10.1126/science.228.4706.1424Roth , C. 2011 Sicherheitsdatenblatt Gemäß Verordnung (EG) Nr. 1907/2006 RepSchmitt, D., Taylor, H. E., Aiken, G. R., Roth, D. A., & Frimmel, F. H. (2002). Influence of Natural Organic Matter on the Adsorption of Metal Ions onto Clay Minerals. Environmental Science & Technology, 36(13), 2932-2938. doi:10.1021/es010271pSix, J., Elliott, E. T., & Paustian, K. (1999). Aggregate and Soil Organic Matter Dynamics under Conventional and No-Tillage Systems. Soil Science Society of America Journal, 63(5), 1350-1358. doi:10.2136/sssaj1999.6351350xStolpe, B., Guo, L., Shiller, A. M., & Hassellöv, M. (2010). Size and composition of colloidal organic matter and trace elements in the Mississippi River, Pearl River and the northern Gulf of Mexico, as characterized by flow field-flow fractionation. Marine Chemistry, 118(3-4), 119-128. doi:10.1016/j.marchem.2009.11.007Tipping, E., & Hurley, M. . (1992). A unifying model of cation binding by humic substances. Geochimica et Cosmochimica Acta, 56(10), 3627-3641. doi:10.1016/0016-7037(92)90158-fTombácz, E., Libor, Z., Illés, E., Majzik, A., & Klumpp, E. (2004). The role of reactive surface sites and complexation by humic acids in the interaction of clay mineral and iron oxide particles. Organic Geochemistry, 35(3), 257-267. doi:10.1016/j.orggeochem.2003.11.002Trostle, K. D., Ray Runyon, J., Pohlmann, M. A., Redfield, S. E., Pelletier, J., McIntosh, J., & Chorover, J. (2016). Colloids and organic matter complexation control trace metal concentration-discharge relationships in Marshall Gulch stream waters. Water Resources Research, 52(10), 7931-7944. doi:10.1002/2016wr019072U.S. Department of Agriculture 1993 Soil survey manual, chapter 3. Selected chemical propertiesVitousek, P. (1982). Nutrient Cycling and Nutrient Use Efficiency. The American Naturalist, 119(4), 553-572. doi:10.1086/283931Wells, M. L., & Goldberg, E. D. (1991). Occurrence of small colloids in sea water. Nature, 353(6342), 342-344. doi:10.1038/353342a0Wen, L.-S., Santschi, P., Gill, G., & Paternostro, C. (1999). Estuarine trace metal distributions in Galveston Bay: importance of colloidal forms in the speciation of the dissolved phase. Marine Chemistry, 63(3-4), 185-212. doi:10.1016/s0304-4203(98)00062-0Zirkler, D., Lang, F., & Kaupenjohann, M. (2012). «Lost in filtration»—The separation of soil colloids from larger particles. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 399, 35-40. doi:10.1016/j.colsurfa.2012.02.02

Whole body magnetic resonance imaging in newly diagnosed multiple myeloma: early changes in lesional signal fat fraction predict disease response

Cross-sectional imaging techniques are being increasingly used for disease evaluation in patients with multiple myeloma. Whole body magnetic resonance imaging (WB-MRI) scanning is superior to plain radiography in baseline assessment of patients but changes following treatment have not been systematically explored. We carried out paired WB-MRI scans in 21 newly diagnosed patients prior to, and 8-weeks after, starting chemotherapy, and analysed stringently selected focal lesions (FLs) for parametric changes. A total of 323 FLs were evaluated, median 20 per patient. At 8 weeks, there was a reduction in estimated tumour volume (eTV), and an increase in signal fat fraction (sFF) and apparent diffusion coefficient (ADC) in the group as a whole (P < 0·001). Patients who achieved complete/very good partial response (CR/VGPR) to induction had a significantly greater increase in sFF compared to those achieving ≤ partial response (PR; P = 0·001). When analysed on a per-patient basis, all patients achieving CR/VGPR had a significant sFF increase in their FL's, in contrast to patients achieving ≤PR. sFF changes in patients reaching maximal response within 100 days (fast responders) were greater compared to slow responders (P = 0·001). Receiver Operator Characteristic analysis indicated that sFF changes at 8 weeks were the best biomarker (area under the Curve 0·95) for an inferior response (≤PR). We conclude that early lesional sFF changes may provide important information on depth of response, and are worthy of further prospective study