Swine embryo culture and transfer for export to England

A major threat to swine enterprises is the possible introduction of disease when new breeding animals are purchased and introduced. So, methods of introducing new genetic material while minimizing the potential for introducing disease are needed. Transfer of embryos from a donor sow in another herd or country would minimize disease risks. Already used to introduce new breeding stock into Specific Pathogen Free herds and other closed herds, embryos now are placed in the recipient gilt’s or sow1s uterus within a few hours after their recovery from the donor. That method prevents export and limits application of swine embryo transfer in this country, so we evaluated the feasibility of using an in vitro culture system to store embryos between donor sows and recipient females.; Swine Day, Manhattan, KS, November 8, 197

Conversion of Iodide to Hypoiodous Acid and Iodine in Aqueous Microdroplets Exposed to Ozone

Halides are incorporated into aerosol sea spray, where they start the catalytic destruction of ozone (O3) over the oceans and affect the global troposphere. Two intriguing environmental problems undergoing continuous research are (1) to understand how reactive gas phase molecular halogens are directly produced from inorganic halides exposed to O3 and (2) to constrain the environmental factors that control this interfacial process. This paper presents a laboratory study of the reaction of O3 at variable iodide (I–) concentration (0.010–100 μM) for solutions aerosolized at 25 °C, which reveal remarkable differences in the reaction intermediates and products expected in sea spray for low tropospheric [O3]. The ultrafast oxidation of I– by O3 at the air–water interface of microdroplets is evidenced by the appearance of hypoiodous acid (HIO), iodite (IO2–), iodate (IO3–), triiodide (I3–), and molecular iodine (I2). Mass spectrometry measurements reveal an enhancement (up to 28%) in the dissolution of gaseous O3 at the gas–liquid interface when increasing the concentration of NaI or NaBr from 0.010 to 100 μM. The production of iodine species such as HIO and I2 from NaI aerosolized solutions exposed to 50 ppbv O3 can occur at the air–water interface of sea spray, followed by their transfer to the gas-phase, where they contribute to the loss of tropospheric ozone

The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain

[EN] Phytophthora is one of the most important and aggressive plant pathogenic genera in agriculture and forestry. Early detection and identification of its pathways of infection and spread are of high importance to minimize the threat they pose to natural ecosystems. eDNA was extracted from soil and water from forests and plantations in the north of Spain. Phytophthora-specific primers were adapted for use in high-throughput Sequencing (HTS). Primers were tested in a control reaction containing eight Phytophthora species and applied to water and soil eDNA samples from northern Spain. Different score coverage threshold values were tested for optimal Phytophthora species separation in a custom-curated database and in the control reaction. Clustering at 99% was the optimal criteria to separate most of the Phytophthora species. Multiple Molecular Operational Taxonomic Units (MOTUs) corresponding to 36 distinct Phytophthora species were amplified in the environmental samples. Pyrosequencing of amplicons from soil samples revealed low Phytophthora diversity (13 species) in comparison with the 35 species detected in water samples. Thirteen of the MOTUs detected in rivers and streams showed no close match to sequences in international sequence databases, revealing that eDNA pyrosequencing is a useful strategy to assess Phytophthora species diversity in natural ecosystems.This project has been supported by the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (EUPHRESCO-CEP: "Current and Emerging Phytophthoras: Research Supporting Risk Assessment And Risk Management"). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Català, S.; Pérez Sierra, AM.; Abad Campos, P. (2015). The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain. PLoS ONE. 10(3):1-14. doi:10.1371/journal.pone.0119311S114103REICHARD, S. H., & WHITE, P. (2001). Horticulture as a Pathway of Invasive Plant Introductions in the United States. BioScience, 51(2), 103. doi:10.1641/0006-3568(2001)051[0103:haapoi]2.0.co;2Brasier, C. M. (2008). The biosecurity threat to the UK and global environment from international trade in plants. Plant Pathology, 57(5), 792-808. doi:10.1111/j.1365-3059.2008.01886.xTABERLET, P., COISSAC, E., HAJIBABAEI, M., & RIESEBERG, L. H. (2012). Environmental DNA. Molecular Ecology, 21(8), 1789-1793. doi:10.1111/j.1365-294x.2012.05542.xSogin, M. L., Morrison, H. G., Huber, J. A., Welch, D. M., Huse, S. M., Neal, P. R., … Herndl, G. J. (2006). Microbial diversity in the deep sea and the underexplored «rare biosphere». Proceedings of the National Academy of Sciences, 103(32), 12115-12120. doi:10.1073/pnas.0605127103Roesch, L. F. W., Fulthorpe, R. R., Riva, A., Casella, G., Hadwin, A. K. M., Kent, A. D., … Triplett, E. W. (2007). Pyrosequencing enumerates and contrasts soil microbial diversity. The ISME Journal, 1(4), 283-290. doi:10.1038/ismej.2007.53Acosta-Martínez, V., Dowd, S., Sun, Y., & Allen, V. (2008). Tag-encoded pyrosequencing analysis of bacterial diversity in a single soil type as affected by management and land use. Soil Biology and Biochemistry, 40(11), 2762-2770. doi:10.1016/j.soilbio.2008.07.022Jumpponen, A., & Jones, K. L. (2009). Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperateQuercus macrocarpaphyllosphere. New Phytologist, 184(2), 438-448. doi:10.1111/j.1469-8137.2009.02990.xNilsson, R. H., Ryberg, M., Abarenkov, K., Sjökvist, E., & Kristiansson, E. (2009). The ITS region as a target for characterization of fungal communities using emerging sequencing technologies. FEMS Microbiology Letters, 296(1), 97-101. doi:10.1111/j.1574-6968.2009.01618.xCoince, A., Caël, O., Bach, C., Lengellé, J., Cruaud, C., Gavory, F., … Buée, M. (2013). Below-ground fine-scale distribution and soil versus fine root detection of fungal and soil oomycete communities in a French beech forest. Fungal Ecology, 6(3), 223-235. doi:10.1016/j.funeco.2013.01.002Vannini, A., Bruni, N., Tomassini, A., Franceschini, S., & Vettraino, A. M. (2013). Pyrosequencing of environmental soil samples reveals biodiversity of thePhytophthoraresident community in chestnut forests. FEMS Microbiology Ecology, 85(3), 433-442. doi:10.1111/1574-6941.12132Jerde, C. L., Mahon, A. R., Chadderton, W. L., & Lodge, D. M. (2011). «Sight-unseen» detection of rare aquatic species using environmental DNA. Conservation Letters, 4(2), 150-157. doi:10.1111/j.1755-263x.2010.00158.xMonchy, S., Sanciu, G., Jobard, M., Rasconi, S., Gerphagnon, M., Chabé, M., … Sime-Ngando, T. (2011). Exploring and quantifying fungal diversity in freshwater lake ecosystems using rDNA cloning/sequencing and SSU tag pyrosequencing. Environmental Microbiology, 13(6), 1433-1453. doi:10.1111/j.1462-2920.2011.02444.xJobard, M., Rasconi, S., Solinhac, L., Cauchie, H.-M., & Sime-Ngando, T. (2012). Molecular and morphological diversity of fungi and the associated functions in three European nearby lakes. Environmental Microbiology, 14(9), 2480-2494. doi:10.1111/j.1462-2920.2012.02771.xLivermore, J. A., & Mattes, T. E. (2013). Phylogenetic detection of novel Cryptomycota in an Iowa (United States) aquifer and from previously collected marine and freshwater targeted high-throughput sequencing sets. Environmental Microbiology, 15(8), 2333-2341. doi:10.1111/1462-2920.12106NAKAYAMA, J., JIANG, J., WATANABE, K., CHEN, K., NINXIN, H., MATSUDA, K., … LEE, Y.-K. (2013). Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing. Bioscience of Microbiota, Food and Health, 32(2), 69-76. doi:10.12938/bmfh.32.69CREER, S., & SINNIGER, F. (2012). Cosmopolitanism of microbial eukaryotes in the global deep seas. Molecular Ecology, 21(5), 1033-1035. doi:10.1111/j.1365-294x.2012.05437.xDavey, M. L., Heegaard, E., Halvorsen, R., Kauserud, H., & Ohlson, M. (2012). Amplicon-pyrosequencing-based detection of compositional shifts in bryophyte-associated fungal communities along an elevation gradient. Molecular Ecology, 22(2), 368-383. doi:10.1111/mec.12122Weber, C. F., Vilgalys, R., & Kuske, C. R. (2013). Changes in Fungal Community Composition in Response to Elevated Atmospheric CO2 and Nitrogen Fertilization Varies with Soil Horizon. Frontiers in Microbiology, 4. doi:10.3389/fmicb.2013.00078Bergmark, L., Poulsen, P. H. B., Al-Soud, W. A., Norman, A., Hansen, L. H., & Sørensen, S. J. (2012). Assessment of the specificity of Burkholderia and Pseudomonas qPCR assays for detection of these genera in soil using 454 pyrosequencing. FEMS Microbiology Letters, 333(1), 77-84. doi:10.1111/j.1574-6968.2012.02601.xLi, L., Abu Al-Soud, W., Bergmark, L., Riber, L., Hansen, L. H., Magid, J., & Sørensen, S. J. (2013). Investigating the Diversity of Pseudomonas spp. in Soil Using Culture Dependent and Independent Techniques. Current Microbiology, 67(4), 423-430. doi:10.1007/s00284-013-0382-xSCHENA, L., HUGHES, K. J. D., & COOKE, D. E. L. (2006). Detection and quantification ofPhytophthora ramorum,P. kernoviae,P. citricolaandP. quercinain symptomatic leaves by multiplex real-time PCR. Molecular Plant Pathology, 7(5), 365-379. doi:10.1111/j.1364-3703.2006.00345.xTooley, P. W., Martin, F. N., Carras, M. M., & Frederick, R. D. (2006). Real-Time Fluorescent Polymerase Chain Reaction Detection ofPhytophthora ramorumandPhytophthora pseudosyringaeUsing Mitochondrial Gene Regions. Phytopathology, 96(4), 336-345. doi:10.1094/phyto-96-0336Pavón, C. F., Babadoost, M., & Lambert, K. N. (2008). Quantification of Phytophthora capsici Oospores in Soil by Sieving-Centrifugation and Real-Time Polymerase Chain Reaction. Plant Disease, 92(1), 143-149. doi:10.1094/pdis-92-1-0143Than, D. J., Hughes, K. J. D., Boonhan, N., Tomlinson, J. A., Woodhall, J. W., & Bellgard, S. E. (2013). A TaqMan real-time PCR assay for the detection ofPhytophthora‘taxon Agathis’ in soil, pathogen of Kauri in New Zealand. Forest Pathology, 43(4), 324-330. doi:10.1111/efp.12034Chen, W., Djama, Z. R., Coffey, M. D., Martin, F. N., Bilodeau, G. J., Radmer, L., … Lévesque, C. A. (2013). Membrane-Based Oligonucleotide Array Developed from Multiple Markers for the Detection of Many Phytophthora Species. Phytopathology, 103(1), 43-54. doi:10.1094/phyto-04-12-0092-rScibetta, S., Schena, L., Chimento, A., Cacciola, S. O., & Cooke, D. E. L. (2012). A molecular method to assess Phytophthora diversity in environmental samples. Journal of Microbiological Methods, 88(3), 356-368. doi:10.1016/j.mimet.2011.12.012Català S, Pérez-Sierra A, Berbegal M, Abad-Campos P. First approach into the knowledge of the Phytophthora species diversity in Mediterranean holm oak forests based on 454 parallel amplicon pyrosequencing of soil samples. Phytophthora in Forest and Natural Ecosystems 6th International IUFRO Working Party 7.02.09 Meeting, Córdoba, Spain, pp 34; 2012.Català S, Pérez-Sierra A, Beltrán A, Abad-Campos P. Next Generation Sequencing shows Phytophthora species diversity in soil samples of Macaronesian laurel forests from the Canary Islands. Phytophthora in Forest and Natural Ecosystems 6th International IUFRO Working Party 7.02.09 Meeting, Córdoba, Spain, pp. 86; 2012.Cooke, D. E. L., Drenth, A., Duncan, J. M., Wagels, G., & Brasier, C. M. (2000). A Molecular Phylogeny of Phytophthora and Related Oomycetes. Fungal Genetics and Biology, 30(1), 17-32. doi:10.1006/fgbi.2000.1202Andrews S. FastQC: a quality control tool for high throughput sequence data. Available: http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/Chou, H.-H., & Holmes, M. H. (2001). DNA sequence quality trimming and vector removal. Bioinformatics, 17(12), 1093-1104. doi:10.1093/bioinformatics/17.12.1093Altschul, S. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389-3402. doi:10.1093/nar/25.17.3389Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5), 1792-1797. doi:10.1093/nar/gkh340Gouy, M., Guindon, S., & Gascuel, O. (2009). SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building. Molecular Biology and Evolution, 27(2), 221-224. doi:10.1093/molbev/msp259Park, J., Park, B., Veeraraghavan, N., Jung, K., Lee, Y.-H., Blair, J. E., … Kang, S. (2008). Phytophthora Database: A Forensic Database Supporting the Identification and Monitoring of Phytophthora. Plant Disease, 92(6), 966-972. doi:10.1094/pdis-92-6-0966Vettraino, A. M., Bonants, P., Tomassini, A., Bruni, N., & Vannini, A. (2012). Pyrosequencing as a tool for the detection ofPhytophthoraspecies: error rate and risk of false Molecular Operational Taxonomic Units. Letters in Applied Microbiology, 55(5), 390-396. doi:10.1111/j.1472-765x.2012.03310.xJung, T., & Burgess, T. I. (2009). Re-evaluation of Phytophthora citricola isolates from multiple woody hosts in Europe and North America reveals a new species, Phytophthora plurivora sp. nov. Persoonia - Molecular Phylogeny and Evolution of Fungi, 22(1), 95-110. doi:10.3767/003158509x442612Deagle, B. E., Eveson, J. P., & Jarman, S. N. (2006). Quantification of damage in DNA recovered from highly degraded samples – a case study on DNA in faeces. Frontiers in Zoology, 3(1). doi:10.1186/1742-9994-3-11Dejean, T., Valentini, A., Duparc, A., Pellier-Cuit, S., Pompanon, F., Taberlet, P., & Miaud, C. (2011). Persistence of Environmental DNA in Freshwater Ecosystems. PLoS ONE, 6(8), e23398. doi:10.1371/journal.pone.0023398Guha Roy S, Grunwald NJ. The plant destroyer genus Phytophthora in the 21st century. In book: Review of Plant Pathology, Edition: Volume 6, Publisher: Scientific Publishers (India), Jodhpur, Editors: B.N.Chakraborty, B.B.L.Thakore, pp. In press; 2014.Brasier, C. M., Cooke, D. E. L., Duncan, J. M., & Hansen, E. M. (2003). Multiple new phenotypic taxa from trees and riparian ecosystems in Phytophthora gonapodyides-P. megasperma ITS Clade 6, which tend to be high-temperature tolerant and either inbreeding or sterile. Mycological Research, 107(3), 277-290. doi:10.1017/s095375620300738xHüberli, D., Hardy, G. E. S. J., White, D., Williams, N., & Burgess, T. I. (2013). Fishing for Phytophthora from Western Australia’s waterways: a distribution and diversity survey. Australasian Plant Pathology, 42(3), 251-260. doi:10.1007/s13313-012-0195-6Jung, T., Stukely, M. J. C., Hardy, G. E. S. J., White, D., Paap, T., Dunstan, W. A., & Burgess, T. I. (2011). Multiple new Phytophthora species from ITS Clade 6 associated with natural ecosystems in Australia: evolutionary and ecological implications. Persoonia - Molecular Phylogeny and Evolution of Fungi, 26(1), 13-39. doi:10.3767/003158511x557577Brasier, C. M., Sanchez-Hernandez, E., & Kirk, S. A. (2003). Phytophthora inundata sp. nov., a part heterothallic pathogen of trees and shrubs in wet or flooded soils. Mycological Research, 107(4), 477-484. doi:10.1017/s0953756203007548Hansen, E. M., Reeser, P. W., & Sutton, W. (2012). PhytophthoraBeyond Agriculture. Annual Review of Phytopathology, 50(1), 359-378. doi:10.1146/annurev-phyto-081211-172946Reeser, P. W., Sutton, W., Hansen, E. M., Remigi, P., & Adams, G. C. (2011). Phytophthora species in forest streams in Oregon and Alaska. Mycologia, 103(1), 22-35. doi:10.3852/10-013Nechwatal, J., Bakonyi, J., Cacciola, S. O., Cooke, D. E. L., Jung, T., Nagy, Z. Á., … Brasier, C. M. (2012). The morphology, behaviour and molecular phylogeny ofPhytophthorataxon Salixsoil and its redesignation asPhytophthora lacustrissp. nov. Plant Pathology, 62(2), 355-369. doi:10.1111/j.1365-3059.2012.02638.xHuai, W. -x., Tian, G., Hansen, E. M., Zhao, W. -x., Goheen, E. M., Grünwald, N. J., & Cheng, C. (2013). Identification ofPhytophthoraspecies baited and isolated from forest soil and streams in northwestern Yunnan province, China. Forest Pathology, 43(2), 87-103. doi:10.1111/efp.12015Oh, E., Gryzenhout, M., Wingfield, B. D., Wingfield, M. J., & Burgess, T. I. (2013). Surveys of soil and water reveal a goldmine of Phytophthora diversity in South African natural ecosystems. IMA Fungus, 4(1), 123-131. doi:10.5598/imafungus.2013.04.01.1

Meta-analysis of type 2 Diabetes in African Americans Consortium

Type 2 diabetes (T2D) is more prevalent in African Americans than in Europeans. However, little is known about the genetic risk in African Americans despite the recent identification of more than 70 T2D loci primarily by genome-wide association studies (GWAS) in individuals of European ancestry. In order to investigate the genetic architecture of T2D in African Americans, the MEta-analysis of type 2 DIabetes in African Americans (MEDIA) Consortium examined 17 GWAS on T2D comprising 8,284 cases and 15,543 controls in African Americans in stage 1 analysis. Single nucleotide polymorphisms (SNPs) association analysis was conducted in each study under the additive model after adjustment for age, sex, study site, and principal components. Meta-analysis of approximately 2.6 million genotyped and imputed SNPs in all studies was conducted using an inverse variance-weighted fixed effect model. Replications were performed to follow up 21 loci in up to 6,061 cases and 5,483 controls in African Americans, and 8,130 cases and 38,987 controls of European ancestry. We identified three known loci (TCF7L2, HMGA2 and KCNQ1) and two novel loci (HLA-B and INS-IGF2) at genome-wide significance (4.15 × 10(-94)<P<5 × 10(-8), odds ratio (OR)  = 1.09 to 1.36). Fine-mapping revealed that 88 of 158 previously identified T2D or glucose homeostasis loci demonstrated nominal to highly significant association (2.2 × 10(-23) < locus-wide P<0.05). These novel and previously identified loci yielded a sibling relative risk of 1.19, explaining 17.5% of the phenotypic variance of T2D on the liability scale in African Americans. Overall, this study identified two novel susceptibility loci for T2D in African Americans. A substantial number of previously reported loci are transferable to African Americans after accounting for linkage disequilibrium, enabling fine mapping of causal variants in trans-ethnic meta-analysis studies.Peer reviewe

Understanding acute ankle ligamentous sprain injury in sports

This paper summarizes the current understanding on acute ankle sprain injury, which is the most common acute sport trauma, accounting for about 14% of all sport-related injuries. Among, 80% are ligamentous sprains caused by explosive inversion or supination. The injury motion often happens at the subtalar joint and tears the anterior talofibular ligament (ATFL) which possesses the lowest ultimate load among the lateral ligaments at the ankle. For extrinsic risk factors to ankle sprain injury, prescribing orthosis decreases the risk while increased exercise intensity in soccer raises the risk. For intrinsic factors, a foot size with increased width, an increased ankle eversion to inversion strength, plantarflexion strength and ratio between dorsiflexion and plantarflexion strength, and limb dominance could increase the ankle sprain injury risk. Players with a previous sprain history, players wearing shoes with air cells, players who do not stretch before exercising, players with inferior single leg balance, and overweight players are 4.9, 4.3, 2.6, 2.4 and 3.9 times more likely to sustain an ankle sprain injury. The aetiology of most ankle sprain injuries is incorrect foot positioning at landing – a medially-deviated vertical ground reaction force causes an explosive supination or inversion moment at the subtalar joint in a short time (about 50 ms). Another aetiology is the delayed reaction time of the peroneal muscles at the lateral aspect of the ankle (60–90 ms). The failure supination or inversion torque is about 41–45 Nm to cause ligamentous rupture in simulated spraining tests on cadaver. A previous case report revealed that the ankle joint reached 48 degrees inversion and 10 degrees internal rotation during an accidental grade I ankle ligamentous sprain injury during a dynamic cutting trial in laboratory. Diagnosis techniques and grading systems vary, but the management of ankle ligamentous sprain injury is mainly conservative. Immobilization should not be used as it results in joint stiffness, muscle atrophy and loss of proprioception. Traditional Chinese medicine such as herbs, massage and acupuncture were well applied in China in managing sports injuries, and was reported to be effective in relieving pain, reducing swelling and edema, and restoring normal ankle function. Finally, the best practice of sports medicine would be to prevent the injury. Different previous approaches, including designing prophylactice devices, introducing functional interventions, as well as change of games rules were highlighted. This paper allows the readers to catch up with the previous researches on ankle sprain injury, and facilitate the future research idea on sport-related ankle sprain injury

Adaptations to Submarine Hydrothermal Environments Exemplified by the Genome of Nautilia profundicola

Submarine hydrothermal vents are model systems for the Archaean Earth environment, and some sites maintain conditions that may have favored the formation and evolution of cellular life. Vents are typified by rapid fluctuations in temperature and redox potential that impose a strong selective pressure on resident microbial communities. Nautilia profundicola strain Am-H is a moderately thermophilic, deeply-branching Epsilonproteobacterium found free-living at hydrothermal vents and is a member of the microbial mass on the dorsal surface of vent polychaete, Alvinella pompejana. Analysis of the 1.7-Mbp genome of N. profundicola uncovered adaptations to the vent environment—some unique and some shared with other Epsilonproteobacterial genomes. The major findings included: (1) a diverse suite of hydrogenases coupled to a relatively simple electron transport chain, (2) numerous stress response systems, (3) a novel predicted nitrate assimilation pathway with hydroxylamine as a key intermediate, and (4) a gene (rgy) encoding the hallmark protein for hyperthermophilic growth, reverse gyrase. Additional experiments indicated that expression of rgy in strain Am-H was induced over 100-fold with a 20°C increase above the optimal growth temperature of this bacterium and that closely related rgy genes are present and expressed in bacterial communities residing in geographically distinct thermophilic environments. N. profundicola, therefore, is a model Epsilonproteobacterium that contains all the genes necessary for life in the extreme conditions widely believed to reflect those in the Archaean biosphere—anaerobic, sulfur, H2- and CO2-rich, with fluctuating redox potentials and temperatures. In addition, reverse gyrase appears to be an important and common adaptation for mesophiles and moderate thermophiles that inhabit ecological niches characterized by rapid and frequent temperature fluctuations and, as such, can no longer be considered a unique feature of hyperthermophiles

Motivation and satisfaction of volunteers for community-based urban agriculture programmes

Urban agriculture means cultivating plants and raising livestock within cities for food and other uses. A Community‐based Urban Agriculture Programme is where people from residential areas get together as volunteers to practise urban agriculture in an empty space within residential areas. However, the programme encounters problems when it is incapable of attracting enough volunteers and retaining them in order to establish a sustainable programme. This study aims to determine the relationship between the dimensions of motivation and satisfaction of volunteers on the Community‐based Urban Agriculture Programme. Data collected from 375 volunteers on the Community‐based Urban Agriculture Programme in Klang Valley, Malaysia were analysed using descriptive analysis, reliability analysis, correlation analysis, and hierarchical multiple regression analysis. It was found that the most significant predictor of Community‐based Urban Agriculture Programme volunteers’ satisfaction was favoured by external factors such as campaigns, support groups, Department of Extension, and community as well as government policy, followed by love of farming, social referents, and values. Therefore, there should be a focus on the above‐mentioned dimensions of motivation in order to enhance the satisfaction of volunteers towards the Community‐based Urban Agriculture Programme