18 research outputs found
Diverse Roles and Interactions of the SWI/SNF Chromatin Remodeling Complex Revealed Using Global Approaches
A systems understanding of nuclear organization and events is critical for determining how cells divide, differentiate, and respond to stimuli and for identifying the causes of diseases. Chromatin remodeling complexes such as SWI/SNF have been implicated in a wide variety of cellular processes including gene expression, nuclear organization, centromere function, and chromosomal stability, and mutations in SWI/SNF components have been linked to several types of cancer. To better understand the biological processes in which chromatin remodeling proteins participate, we globally mapped binding regions for several components of the SWI/SNF complex throughout the human genome using ChIP-Seq. SWI/SNF components were found to lie near regulatory elements integral to transcription (e.g. 5′ ends, RNA Polymerases II and III, and enhancers) as well as regions critical for chromosome organization (e.g. CTCF, lamins, and DNA replication origins). Interestingly we also find that certain configurations of SWI/SNF subunits are associated with transcripts that have higher levels of expression, whereas other configurations of SWI/SNF factors are associated with transcripts that have lower levels of expression. To further elucidate the association of SWI/SNF subunits with each other as well as with other nuclear proteins, we also analyzed SWI/SNF immunoprecipitated complexes by mass spectrometry. Individual SWI/SNF factors are associated with their own family members, as well as with cellular constituents such as nuclear matrix proteins, key transcription factors, and centromere components, implying a ubiquitous role in gene regulation and nuclear function. We find an overrepresentation of both SWI/SNF-associated regions and proteins in cell cycle and chromosome organization. Taken together the results from our ChIP and immunoprecipitation experiments suggest that SWI/SNF facilitates gene regulation and genome function more broadly and through a greater diversity of interactions than previously appreciated
Decline in seasonal predictability potentially destabilized Classic Maya societies
Classic Maya populations living in peri-urban states were highly dependent on seasonally distributed rainfall for reliable surplus crop yields. Despite intense study of the potential impact of decadal to centennial-scale climatic changes on the demise of Classic Maya sociopolitical institutions (750-950 CE), its direct importance remains debated. We provide a detailed analysis of a precisely dated speleothem record from Yok Balum cave, Belize, that reflects local hydroclimatic changes at seasonal scale over the past 1600 years. We find that the initial disintegration of Maya sociopolitical institutions and population decline occurred in the context of a pronounced decrease in the predictability of seasonal rainfall and severe drought between 700 and 800 CE. The failure of Classic Maya societies to successfully adapt to volatile seasonal rainfall dynamics likely contributed to gradual but widespread processes of sociopolitical disintegration. We propose that the complex abandonment of Classic Maya population centres was not solely driven by protracted drought but also aggravated by year-to-year decreases in rainfall predictability, potentially caused by a regional reduction in coherent Intertropical Convergence Zone-driven rainfall