84 research outputs found
Evaluation of Enzyme Effectiveness at Enhancing Fiber Digestion in Small Ruminants
Feed costs account for up to 70% of total production costs making it vital that livestock producers maximize feed efficiency in a cost-effective manner (Bach, 2012). Producers often utilize feed additives to optimize nutrient absorption and increase productivity (McGrath et al., 2018). This study was conducted to determine the effects of an enzyme supplement containing Aspergillus niger and Aspergillus oryzae on dry matter (DM), organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) intake and digestibility by lambs fed ad libitum diets of either alfalfa or bermudagrass hay. The study was set up in a 2 2 factorial treatment arrangement in which 20 lambs were weighted, stratified by weight, then randomly assigned to one of the four treatments: 1) alfalfa hay plus enzyme, 2) alfalfa hay with no enzyme, 3) bermudagrass hay plus enzyme, and 4) bermudagrass hay with no enzyme. Intake or digestibility of DM, OM, NDF, or ADF were not affected (P ≥ 0.42) by enzyme supplementation. Intake of DM and OM and digestibility of DM were greater (P \u3c 0.05) from lambs offered alfalfa than from those offered bermudagrass, resulting in greater (P \u3c 0.05) digestible DM and OM intake by lambs offered alfalfa hay. Intake of NDF or ADF was not different (P ≥ 0.21) between forages, but NDF and ADF digestibility was greater in lambs fed alfalfa diets (P \u3c 0.01). Ruminal pH was greater in lambs offered enzyme (P \u3c 0.05). The enzyme had no effect on total or individual ruminal VFA concentrations (P ≥ 0.10), but total ruminal VFA concentrations were higher, and acetate concentrations were lower in lambs offered alfalfa (P \u3c 0.05). Forage by time interactions were observed for all individual ruminal VFA concentrations with the exception of acetate (P \u3c 0.05). Therefore, the Aspergillus enzyme blend increased ruminal pH but had no effects on intake or digestibility of alfalfa or bermudagrass hay
Complex cytogenetic rearrangements at the DURS1 locus in syndromic Duane retraction syndrome
Key Clinical Message A patient with syndromic Duane retraction syndrome harbors a chromosome 811.1q13.2 inversion and 8p11.1-q12.3 marker chromosome containing subregions with differing mosaicism and allele frequencies. This case highlights the potential requirement for multiple genetic methods to gain insight into genotype–phenotype correlation, and ultimately into molecular mechanisms that underlie human disease
Nitrogen Loss Estimation Worksheet (NLEW): An Agricultural Nitrogen Loading Reduction Tracking Tool
The Neuse River Basin in North Carolina was regulated in 1998, requiring that all pollution sources (point and nonpoint) reduce nitrogen (N) loading into the Neuse Estuary by 30%. Point source N reductions have already been reduced by approximately 35%. The diffuse nature of nonpoint source pollution, and its spatial and temporal variability, makes it a more difficult problem to treat. Agriculture is believed to contribute over 50% of the total N load to the river. In order to reduce these N inputs, best management practices (BMPs) are necessary to control the delivery of N from agricultural activities to water resources and to prevent impacts to the physical and biological integrity of surface and ground water. To provide greater flexibility to the agricultural community beyond standard BMPs (nutrient management, riparian buffers, and water-control structures), an agricultural N accounting tool, called Nitrogen Loss Estimation Worksheet (NLEW), was developed to track N reductions due to BMP implementation. NLEW uses a modified N-balance equation that accounts for some N inputs as well as N reductions from nutrient management and other BMPs. It works at both the field- and county-level scales. The tool has been used by counties to determine different N reduction strategies to achieve the 30% targeted reduction
Consorciação de plantas de cobertura antecedendo o milho em plantio direto: I - Dinâmica do nitrogênio no solo
Winter Rye Cover Crop Biomass Production, Degradation, and Nitrogen Recycling
Winter rye (Secale cereale L.) cover crop (RCC) use in corn (Zea mays L.) and soybean [Glycine max. (L.) Merr.] production can alter N dynamics compared to no RCC. The objectives of this study were to evaluate RCC biomass production (BP) and subsequent RCC degradation (BD) and N recycling in a no-till corn–soybean (CS) rotation. Aboveground RCC was sampled at spring termination for biomass dry matter (DM), C, and N. To evaluate BD and remaining C and N, RCC biomass was put into nylon mesh bags, placed on the soil surface, and collected multiple times over 105 d. Treatments included rye cover crop following soybean (RCC-FS) and corn (RCC-FC), and prior-year N applied to corn. Overall, the RCC BP and N was low due to low soil profile NO3–N. Across sites and years, the greatest BP was with RCC-FC that received 225 kg N ha–1 (1280 kg DM ha–1), with similar N uptake as with RCC-FS (27 kg N ha–1). The RCC biomass and N remaining decreased over time following an exponential decay. An average 62% biomass with RCC-FS and RCC-FC degraded after 105 d; however, N recycled was greater with RCC-FS than RCC-FC [22 (80%) vs. 14 (64%) kg N ha–1, respectively], and was influenced by the RCC C/N ratio. The RCC did not recycle an agronomically meaningful amount of N, which limited N that could potentially be supplied to corn. Rye cover crops can conserve soil N, and with improved management and growth, recycling of crop-available N should increase
Disruptive variants of CSDE1 associate with autism and interfere with neuronal development and synaptic transmission
Nutrição e produtividade da cultura do milho em sistemas de culturas e fontes de adubação
Clinical and molecular features of 66 patients with musculocontractural Ehlers-Danlos syndrome caused by pathogenic variants in CHST14 (mcEDS-CHST14)
Background Musculocontractural Ehlers-Danlos syndrome is caused by biallelic loss-of-function variants in CHST14 (mcEDS-CHST14) or DSE (mcEDS-DSE). Although 48 patients in 33 families with mcEDS-CHST14 have been reported, the spectrum of pathogenic variants, accurate prevalence of various manifestations and detailed natural history have not been systematically investigated. Methods We collected detailed and comprehensive clinical and molecular information regarding previously reported and newly identified patients with mcEDS-CHST14 through international collaborations. Results Sixty-six patients in 48 families (33 males/females; 0-59 years), including 18 newly reported patients, were evaluated. Japanese was the predominant ethnicity (27 families), associated with three recurrent variants. No apparent genotype-phenotype correlation was noted. Specific craniofacial (large fontanelle with delayed closure, downslanting palpebral fissures and hypertelorism), skeletal (characteristic finger morphologies, joint hypermobility, multiple congenital contractures, progressive talipes deformities and recurrent joint dislocation), cutaneous (hyperextensibility, fine/acrogeria-like/wrinkling palmar creases and bruisability) and ocular (refractive errors) features were observed in most patients (>90%). Large subcutaneous haematomas, constipation, cryptorchidism, hypotonia and motor developmental delay were also common (>80%). Median ages at the initial episode of dislocation or large subcutaneous haematoma were both 6 years. Nine patients died; their median age was 12 years. Several features, including joint and skin characteristics (hypermobility/extensibility and fragility), were significantly more frequent in patients with mcEDS-CHST14 than in eight reported patients with mcEDS-DSE. Conclusion This first international collaborative study of mcEDS-CHST14 demonstrated that the subtype represents a multisystem disorder with unique set of clinical phenotypes consisting of multiple malformations and progressive fragility-related manifestations; these require lifelong, multidisciplinary healthcare approaches.Genetics of disease, diagnosis and treatmen
Corn Nitrogen Fertilization Requirement and Corn-Soybean Productivity with a Rye Cover Crop
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