Análisis del uso de los osteodilatadores para la creación del lecho implantológico: aportaciones técnicas y revisión de la literatura

La atrofia ósea severa del maxilar superior compromete e incluso, en ocasiones, impide la colocación de implantes. Con la expansión ósea mediante osteodilatadores se evita usar instrumentos rotatorios, no se produce calor ni se elimina hueso y se simplifica el tratamiento quirúrgico permitiendo la colocación de fijaciones sin recurrir a tratamientos más complejos. Presentamos una revisión de la técnica de expansión maxilar mediante osteodilatadores, describiendo las principales indicaciones, ventajas e inconvenientes de este procediminiento quirúrgico.Sometimes, the severe superior maxillary atrophy compromises and even impide oral implants treatment. The use of rotatory instruments (drills) produce heat and lost of bone, but the osteotomes in implantology avoid this and simplifies the surgical treatment allowing implant installation with an easier technique. We present an expansive maxillary technique review, describing the main indications, advantages and disadvantages of this surgical procedure

Analysis of the use of expansion osteotomes for the creation of implant beds : Technical contributions and review of the literature

La atrofia ósea severa del maxilar superior compromete e incluso, en ocasiones, impide la colocación de implantes. Con la expansión ósea mediante osteodilatadores se evita usar instrumentos rotatorios, no se produce calor ni se elimina hueso y se simplifica el tratamiento quirúrgico permitiendo la colocación de fijaciones sin recurrir a tratamientos más complejos. Presentamos una revisión de la técnica de expansión maxilar mediante osteodilatadores, describiendo las principales indicaciones, ventajas e inconvenientes de este procediminiento quirúrgico

Murcy, una aplicación web para crear y responder preguntas de forma lúdica

El objetivo de este proyecto es la evolución y expansión de Murcy, una prueba de concepto que permite crear y jugar quizzes. Se desea que Murcy se convierta en un producto mínimo viable para desarrollar pruebas con usuarios reales. Para llevar a cabo esta evolución, se ha realizado un análisis de las alternativas existentes en el mercado para poder extraer nuevas funcionalidades para Murcy, los riesgos existentes y la mejor metodología de desarrollo para Murcy. Y gracias a este análisis se ha podido terminar el proyecto con resultados satisfactorios.Gracias a todo el proceso realizado se ha podido completar la evolución de Murcy a un producto mínimo viable con que realizar pruebas con usuarios reales.<br /

Fast determination of 40 drugs in water using large volume direct injection liquid chromatography–tandem mass spectrometry

This work describes a rapid analytical method based on direct sample injection of water samples for the simultaneous identification/quantification of 40 emerging compounds, including pharmaceuticals and drugs of abuse. The water samples were analyzed by ultra-high-performance liquid chromatography coupled to hybrid triple quadrupole mass spectrometer (UHPLC–MS/MS QqQ). Taking profit of the increasing sensitivity of nowadays tandem mass spectrometers, direct sample injection of large volumes has been an attractive alternative to pre-concentration steps. In this work, the developed methodology has been validated at three concentration levels (10, 100 and 1000 ng/L) in 10 different water samples of different types (5 effluent wastewater and 5 surface water samples). The majority of compounds could be satisfactory validated at these concentrations, showing good recoveries and precision. With only few exceptions, the limits of quantification (LOQs), estimated from the sample chromatogram at lowest spiked level tested, were below 3 ng/L. The method was applied to the analysis of 10 effluent wastewater and 10 surface water samples. Venlafaxine was the compound most frequently detected (80%) in surface water, followed by acetaminophen (70%). Regarding effluent wastewater, valsartan and 4-acetyl aminoantipyrine were detected in 9 out of 10 samples analyzed. These two compounds together with 4-formyl aminoantipyrine and naproxen showed the highest concentrations (>2000 ng/L). In these cases, a dilution step was required for a correct quantification. As an additional evaluation of the method performance, the same water samples were analyzed in another laboratory by a second analytical methodology, based on on-line solid-phase-extraction coupled to LC–MS/MS (QqQ).The authors are very grateful to Serveis Centrals d׳Instrumentació Científica (SCIC) of University Jaume I for using the Xevo TQS mass spectrometer. The financial support of the Centro de Desarrollo Tecnológico Industrial (CDTIIDI-20110823/EXP00044257), of the Spanish Ministry of Education and Science (Ref CTQ2012-36189) and of Generalitat Valenciana (research group of excellence PROMETEO/2009/054; Collaborative Research on Environment and Food Safety, ISIC/2012/016) is acknowledged

Fine-Tuning Tomato Agronomic Properties by Computational Genome Redesign

Considering cells as biofactories, we aimed to optimize its internal processes by using the same engineering principles that large industries are implementing nowadays: lean manufacturing. We have applied reverse engineering computational methods to transcriptomic, metabolomic and phenomic data obtained from a collection of tomato recombinant inbreed lines to formulate a kinetic and constraint-based model that efficiently describes the cellular metabolism from expression of a minimal core of genes. Based on predicted metabolic profiles, a close association with agronomic and organoleptic properties of the ripe fruit was revealed with high statistical confidence. Inspired in a synthetic biology approach, the model was used for exploring the landscape of all possible local transcriptional changes with the aim of engineering tomato fruits with fine-tuned biotechnological properties. The method was validated by the ability of the proposed genomes, engineered for modified desired agronomic traits, to recapitulate experimental correlations between associated metabolites

European traditional tomatoes galore: a result of farmers' selection of a few diversity-rich loci

A comprehensive collection of 1254 tomato accessions, corresponding to European traditional and modern varieties, early domesticated varieties, and wild relatives, was analyzed by genotyping by sequencing. A continuous genetic gradient between the traditional and modern varieties was observed. European traditional tomatoes displayed very low genetic diversity, with only 298 polymorphic loci (95% threshold) out of 64 943 total variants. European traditional tomatoes could be classified into several genetic groups. Two main clusters consisting of Spanish and Italian accessions showed higher genetic diversity than the remaining varieties, suggesting that these regions might be independent secondary centers of diversity with a different history. Other varieties seem to be the result of a more recent complex pattern of migrations and hybridizations among the European regions. Several polymorphic loci were associated in a genome-wide association study with fruit morphological traits in the European traditional collection. The corresponding alleles were found to contribute to the distinctive phenotypic characteristic of the genetic varietal groups. The few highly polymorphic loci associated with morphological traits in an otherwise a low-diversity population suggests a history of balancing selection, in which tomato farmers likely maintained the morphological variation by inadvertently applying a high selective pressure within different varietal types

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Carotenoids are C40 isoprenoids with well-established roles in photosynthesis, pollination, photoprotection, and hormone biosynthesis. The enzymatic or ROS-induced cleavage of carotenoids generates a group of compounds named apocarotenoids, with an increasing interest by virtue of their metabolic, physiological, and ecological activities. Both classes are used industrially in a variety of fields as colorants, supplements, and bio-actives. Crocins and picrocrocin, two saffron apocarotenoids, are examples of high-value pigments utilized in the food, feed, and pharmaceutical industries. In this study, a unique construct was achieved, namely O6, which contains CsCCD2L, UGT74AD1, and UGT709G1 genes responsible for the biosynthesis of saffron apocarotenoids driven by a patatin promoter for the generation of potato tubers producing crocins and picrocrocin. Different tuber potatoes accumulated crocins and picrocrocin ranging from 19.41-360 to 105-800 mu g/g DW, respectively, with crocetin, crocin 1 [(crocetin-(beta-D-glucosyl)-ester)] and crocin 2 [(crocetin)-(beta-D-glucosyl)-(beta-D-glucosyl)-ester)] being the main compounds detected. The pattern of carotenoids and apocarotenoids were distinct between wild type and transgenic tubers and were related to changes in the expression of the pathway genes, especially from PSY2, CCD1, and CCD4. In addition, the engineered tubers showed higher antioxidant capacity, up to almost 4-fold more than the wild type, which is a promising sign for the potential health advantages of these lines. In order to better investigate these aspects, different cooking methods were applied, and each process displayed a significant impact on the retention of apocarotenoids. More in detail, the in vitro bioaccessibility of these metabolites was found to be higher in boiled potatoes (97.23%) compared to raw, baked, and fried ones (80.97, 78.96, and 76.18%, respectively). Overall, this work shows that potatoes can be engineered to accumulate saffron apocarotenoids that, when consumed, can potentially offer better health benefits. Moreover, the high bioaccessibility of these compounds revealed that potato is an excellent way to deliver crocins and picrocrocin, while also helping to improve its nutritional value

Fortification and bioaccessibility of saffron apocarotenoids in potato tubers

Carotenoids are C40 isoprenoids with well-established roles in photosynthesis, pollination, photoprotection, and hormone biosynthesis. The enzymatic or ROS-induced cleavage of carotenoids generates a group of compounds named apocarotenoids, with an increasing interest by virtue of their metabolic, physiological, and ecological activities. Both classes are used industrially in a variety of fields as colorants, supplements, and bio-actives. Crocins and picrocrocin, two saffron apocarotenoids, are examples of high-value pigments utilized in the food, feed, and pharmaceutical industries. In this study, a unique construct was achieved, namely O6, which contains CsCCD2L, UGT74AD1, and UGT709G1 genes responsible for the biosynthesis of saffron apocarotenoids driven by a patatin promoter for the generation of potato tubers producing crocins and picrocrocin. Different tuber potatoes accumulated crocins and picrocrocin ranging from 19.41–360 to 105–800 μg/g DW, respectively, with crocetin, crocin 1 [(crocetin-(β-D-glucosyl)-ester)] and crocin 2 [(crocetin)-(β-D-glucosyl)-(β-D-glucosyl)-ester)] being the main compounds detected. The pattern of carotenoids and apocarotenoids were distinct between wild type and transgenic tubers and were related to changes in the expression of the pathway genes, especially from PSY2, CCD1, and CCD4. In addition, the engineered tubers showed higher antioxidant capacity, up to almost 4-fold more than the wild type, which is a promising sign for the potential health advantages of these lines. In order to better investigate these aspects, different cooking methods were applied, and each process displayed a significant impact on the retention of apocarotenoids. More in detail, the in vitro bioaccessibility of these metabolites was found to be higher in boiled potatoes (97.23%) compared to raw, baked, and fried ones (80.97, 78.96, and 76.18%, respectively). Overall, this work shows that potatoes can be engineered to accumulate saffron apocarotenoids that, when consumed, can potentially offer better health benefits. Moreover, the high bioaccessibility of these compounds revealed that potato is an excellent way to deliver crocins and picrocrocin, while also helping to improve its nutritional value

European traditional tomatoes galore: a result of farmers' selection of a few diversity-rich loci

[EN] The high phenotypic diversity observed among European traditional tomato varieties was created by traditional farmer-driven selection by inadvertently combining a very few polymorphic loci subjected to balancing selection. A comprehensive collection of 1254 tomato accessions, corresponding to European traditional and modern varieties, early domesticated varieties, and wild relatives, was analyzed by genotyping by sequencing. A continuous genetic gradient between the traditional and modern varieties was observed. European traditional tomatoes displayed very low genetic diversity, with only 298 polymorphic loci (95% threshold) out of 64 943 total variants. European traditional tomatoes could be classified into several genetic groups. Two main clusters consisting of Spanish and Italian accessions showed higher genetic diversity than the remaining varieties, suggesting that these regions might be independent secondary centers of diversity with a different history. Other varieties seem to be the result of a more recent complex pattern of migrations and hybridizations among the European regions. Several polymorphic loci were associated in a genome-wide association study with fruit morphological traits in the European traditional collection. The corresponding alleles were found to contribute to the distinctive phenotypic characteristic of the genetic varietal groups. The few highly polymorphic loci associated with morphological traits in an otherwise a low-diversity population suggests a history of balancing selection, in which tomato farmers likely maintained the morphological variation by inadvertently applying a high selective pressure within different varietal types.This work was supported by the European Commission H2020 research and innovation program through TRADITOM grant agreement no. 634561, G2P-SOL, grant agreement no. 677379, and HARNESSTOM grant agreement no. 101000716. MP is grateful to the Spanish Ministerio de Ciencia e Innovacion for a postdoctoral grant (IJC2019-039091-I/AEI/10.13039/501100011033).Blanca Postigo, JM.; Pons Puig, C.; Montero-Pau, J.; Sánchez-Matarredona, D.; Ziarsolo, P.; Fontanet, L.; Fisher, J.... (2022). European traditional tomatoes galore: a result of farmers' selection of a few diversity-rich loci. Journal of Experimental Botany. 73(11):3431-3445. https://doi.org/10.1093/jxb/erac07234313445731