The Effect of The Use of Project Based Learning Models and Flip Based E-Modules on Statistics Materials on Students’ Interest and Learning Outcomes of Class XI TKJ Students at SMK Ifadah

This research is motivated by the students' very low interest in learning, this makes students unmotivated in the learning process. Lack of motivation to actively participate in student learning has little effect on student learning outcomes. PjBL learning model and flip-based e-module media. This is an alternative that will be tested in this study. Researchers use PjBL learning models and flip-based e-module media for alternative learning strategies that can train students' cooperation so that students can increase their interest in learning. This study was to determine whether or not the use of PjBL learning models and flip-based e-module  media on the learning interest of class XI TKJ students in SMK. The type of research used in this research is descriptive quantitative research using One group pretest – posttest research design. The results of this study indicate that there is an effect of using the PjBL model and flip-based e-module media on statistical material on student interest and learning outcomes.Penelitian ini dilatarbelakangi oleh minat belajar peserta didik yang sangat rendah,hal ini membuat siswa tidak termotivasi dalam proses pembelajaran. Kurangnya motivasi untuk berpartisipasi aktif dalam belajar siswa berpengaruh kecil terhadap hasil belajar siswa. Model pembelajaran PjBL dan Media e-modul berbasis flip. Merupakan salah satu alternatif yang akan diuji cobakan pada penelitian ini. Peneliti menggunakan model pembelajaran PjBL dan media e-modul berbasis flip untuk alternatif  strategi pembelajaran yang dapat melatih kerjasama peserta didik sehingga peserta didik dapat meningkatkan minat belajar. penelitian ini adalah untuk mengetahui ada tidaknya pengaruh penggunaan model pembelajaran PjBL dan media e-modul berbasis flip terhadap minat belajar siswa kelas XI TKJ di SMK. Jenis penelitian yang digunakan dalam penelitian ini adalah jenis penelitian deskriptif kuantitatif dengan menggunakan rancangan penelitian One group pretest – posttest. Hasil penelitian ini menjukan bahwa ada pengaruh penggunaan model PjBL dan media e-modul berbasis flip pada materi statistika terhadap minat dan hasil belajar siswa

Genome-wide DNA methylation map of human neutrophils reveals widespread inter-individual epigenetic variation

The extent of variation in DNA methylation patterns in healthy individuals is not yet well documented. Identification of inter-individual epigenetic variation is important for understanding phenotypic variation and disease susceptibility. Using neutrophils from a cohort of healthy individuals, we generated base-resolution DNA methylation maps to document inter-individual epigenetic variation. We identified 12851 autosomal inter-individual variably methylated fragments (iVMFs). Gene promoters were the least variable, whereas gene body and upstream regions showed higher variation in DNA methylation. The iVMFs were relatively enriched in repetitive elements compared to non-iVMFs, and were associated with genome regulation and chromatin function elements. Further, variably methylated genes were disproportionately associated with regulation of transcription, responsive function and signal transduction pathways. Transcriptome analysis indicates that iVMF methylation at differentially expressed exons has a positive correlation and local effect on the inclusion of that exon in the mRNA transcript

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries.

BACKGROUND: As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. METHODS: We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. RESULTS: A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44 814 patients with a median hospital stay of 4 (range 2-7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. CONCLUSIONS: Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. STUDY REGISTRATION: ISRCTN5181700

Plasmonic nanosensors with inverse sensitivity by means of enzyme-guided crystal growth

Lowering the limit of detection is key to the design of sensors needed for food safety regulations1, 2, environmental policies3, 4, 5 and the diagnosis of severe diseases6, 7, 8, 9, 10. However, because conventional transducers generate a signal that is directly proportional to the concentration of the target molecule, ultralow concentrations of the molecule result in variations in the physical properties of the sensor that are tiny, and therefore difficult to detect with confidence. Here we present a signal-generation mechanism that redefines the limit of detection of nanoparticle sensors by inducing a signal that is larger when the target molecule is less concentrated. The key step to achieve this inverse sensitivity is to use an enzyme that controls the rate of nucleation of silver nanocrystals on plasmonic transducers. We demonstrate the outstanding sensitivity and robustness of this approach by detecting the cancer biomarker prostate-specific antigen down to 10−18 g ml−1 (4 × 10−20 M) in whole serum