Determination of tomato quality with hyperspectral imaging

Received: February 1st, 2023 ; Accepted: July 14th, 2023 ; Published: October 13th, 2023 ; Correspondence: [email protected] (Solanum lycopersicum L.) are a widely used vegetable in the human diet throughout the year, both fresh and in various processed products. Tomatoes contain compounds important to human health and are an important source of vitamins, antioxidants, and mineral elements. Performing biochemical analyses is an expensive, environmentally unfriendly and time-consuming process; therefore, a way to determine the biochemical composition of tomatoes using non-destructive methods is being sought. The study includes 45 varieties of tomatoes with different colors - red, pink, orange, brown, yellow, and bicolor tomato fruits. The content of dry matter, soluble dry matter, titratable acidity, lycopene, β-carotene, total phenol, and flavonoids was determined by standard biochemical procedure. Reflectance spectrums of tomato fruits were obtained with Remote Sensing Portable Spectroradiometer RS-3500 (Ltd. Spectral Evolution, Haverhill, MA, USA) at the wavelength 350–2,500 nm with a 1 nm interval. In order to determine the content of various biochemical parameters in tomatoes, the vegetation indices found in the literature were used, and new ones were developed. The research demonstrated that the developed vegetative indices allow to detect lycopene and β-carotene content non-destructively. For the determination of the dry matter, soluble solids and phenolic content, indices designed for detecting water content can be used, but their correlation coefficients with chemical methods are moderately high - 0.65, 0.56 and 0.57, respectively. It was found that the best correlation between biochemically detected parameters and vegetation indices is for lycopene > β-carotene > dry matter> total phenols = titratable acidity ≥ soluble solids > taste index > flavonoids

Antioxidative Activity of Ferrocenes Bearing 2,6-Di-Tert-Butylphenol Moieties

The antioxidative activity of ferrocenes bearing either 2,6-di-tert-butylphenol or phenyl groups has been compared using DPPH (1,1-diphenyl-2-picrylhydrazyl) test and in the study of the in vitro impact on lipid peroxidation in rat brain homogenate and on some characteristics of rat liver mitochondria. The results of DPPH test at 20°C show that the activity depends strongly upon the presence of phenolic group but is improved by the influence of ferrocenyl fragment. The activity of N-(3,5-di-tert-butyl-4-hydroxyphenyl)iminomethylferrocene (1), for instance, was 88.4%, which was higher than the activity of a known antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) (48.5%), whereas the activity of N-phenyl-iminomethylferrocene 2 was almost negligible −2.9%. The data obtained demonstrate that the compounds with 2,6-di-tert-butylphenol moiety are significantly more active than the corresponding phenyl analogues in the in vitro study of lipid peroxidation in rat brain homogenate. Ferrocene 1 performs a promising behavior as an antioxidant and inhibits the calcium-dependent swelling of mitochondria. These results allow us to propose the potential cytoprotective (neuroprotective) effect of ditopic compounds containing antioxidant 2,6-di-tert-butylphenol group and redox active ferrocene fragment

The genetic history of admixture across inner Eurasia

This is the author accepted manuscript. The final version is available from Nature Research via the DOI in this record.Data Availability. Genome-wide sequence data of two Botai individuals (BAM format) are available at the European Nucleotide Archive under the accession number PRJEB31152 (ERP113669). Eigenstrat format array genotype data of 763 present-day individuals and 1240K pulldown genotype data of two ancient Botai individuals are available at the Edmond data repository of the Max Planck Society (https://edmond.mpdl.mpg.de/imeji/collection/Aoh9c69DscnxSNjm?q=).The indigenous populations of inner Eurasia, a huge geographic region covering the central Eurasian steppe and the northern Eurasian taiga and tundra, harbor tremendous diversity in their genes, cultures and languages. In this study, we report novel genome-wide data for 763 individuals from Armenia, Georgia, Kazakhstan, Moldova, Mongolia, Russia, Tajikistan, Ukraine, and Uzbekistan. We furthermore report additional damage-reduced genome-wide data of two previously published individuals from the Eneolithic Botai culture in Kazakhstan (~5,400 BP). We find that present-day inner Eurasian populations are structured into three distinct admixture clines stretching between various western and eastern Eurasian ancestries, mirroring geography. The Botai and more recent ancient genomes from Siberia show a decrease in contribution from so-called “ancient North Eurasian” ancestry over time, detectable only in the northern-most “forest-tundra” cline. The intermediate “steppe-forest” cline descends from the Late Bronze Age steppe ancestries, while the “southern steppe” cline further to the South shows a strong West/South Asian influence. Ancient genomes suggest a northward spread of the southern steppe cline in Central Asia during the first millennium BC. Finally, the genetic structure of Caucasus populations highlights a role of the Caucasus Mountains as a barrier to gene flow and suggests a post-Neolithic gene flow into North Caucasus populations from the steppe.Max Planck SocietyEuropean Research Council (ERC)Russian Foundation for Basic Research (RFBR)Russian Scientific FundNational Science FoundationU.S. National Institutes of HealthAllen Discovery CenterUniversity of OstravaCzech Ministry of EducationXiamen UniversityFundamental Research Funds for the Central UniversitiesMES R

The formation of human populations in South and Central Asia

By sequencing 523 ancient humans, we show that the primary source of ancestry in modern South Asians is a prehistoric genetic gradient between people related to early hunter-gatherers of Iran and Southeast Asia. After the Indus Valley Civilization’s decline, its people mixed with individuals in the southeast to form one of the two main ancestral populations of South Asia, whose direct descendants live in southern India. Simultaneously, they mixed with descendants of Steppe pastoralists who, starting around 4000 years ago, spread via Central Asia to form the other main ancestral population. The Steppe ancestry in South Asia has the same profile as that in Bronze Age Eastern Europe, tracking a movement of people that affected both regions and that likely spread the distinctive features shared between Indo-Iranian and Balto-Slavic languages

Same data, different conclusions: Radical dispersion in empirical results when independent analysts operationalize and test the same hypothesis

In this crowdsourced initiative, independent analysts used the same dataset to test two hypotheses regarding the effects of scientists’ gender and professional status on verbosity during group meetings. Not only the analytic approach but also the operationalizations of key variables were left unconstrained and up to individual analysts. For instance, analysts could choose to operationalize status as job title, institutional ranking, citation counts, or some combination. To maximize transparency regarding the process by which analytic choices are made, the analysts used a platform we developed called DataExplained to justify both preferred and rejected analytic paths in real time. Analyses lacking sufficient detail, reproducible code, or with statistical errors were excluded, resulting in 29 analyses in the final sample. Researchers reported radically different analyses and dispersed empirical outcomes, in a number of cases obtaining significant effects in opposite directions for the same research question. A Boba multiverse analysis demonstrates that decisions about how to operationalize variables explain variability in outcomes above and beyond statistical choices (e.g., covariates). Subjective researcher decisions play a critical role in driving the reported empirical results, underscoring the need for open data, systematic robustness checks, and transparency regarding both analytic paths taken and not taken. Implications for organizations and leaders, whose decision making relies in part on scientific findings, consulting reports, and internal analyses by data scientists, are discussed

First results of ex situ conservation of endangered wild plants of Latvia in the National Botanic Garden

Abstract In 1981, the National Botanic Garden of Latvia began the ex situ conservation of rare and endangered native plants. About 70 species of endangered plants of Latvia are now successfully grown in vitro. The aim of this study was to determine suitable conditions for ex situ cultivation in the territory of the National Botanic Garden, where four artificial habitats (dune and meadow, deciduous tree forest, humid bank and ditch with spring water) were created or used for growth and survival experiments with individuals of 23 endangered wild plant species. Twenty of the species successfully adapted to the implemented growth conditions. Alyssum gmelini, Dianthus arenarius, Helianthemum nummularium showed good adaptation in dry and sunny conditions of artificial dune. Galium schultesii, Pulmonaria angustifolia, Scrophularia umbrosa showed good adaptation to fertile soil and shady conditions of deciduous forest. The short-lived species as Spergularia salina and Tripolium vulgare were identified as difficult species for cultivation