1,078 research outputs found
Tracking autophagy during proliferation and differentiation of trypanosoma brucei
Autophagy is a lysosome-dependent degradation mechanism that sequesters target cargo into autophagosomal vesicles. The Trypanosoma brucei genome contains apparent orthologues of several autophagy-related proteins including an ATG8 family. These ubiquitin-like proteins are required for autophagosome membrane formation, but our studies show that ATG8.3 is atypical. To investigate the function of other ATG proteins, RNAi compatible T. brucei were modified to function as autophagy reporter lines by expressing only either YFP-ATG8.1 or YFP-ATG8.2. In the insect procyclic lifecycle stage, independent RNAi down-regulation of ATG3 or ATG7 generated autophagy-defective mutants and confirmed a pro-survival role for autophagy in the procyclic form nutrient starvation response. Similarly, RNAi depletion of ATG5 or ATG7 in the bloodstream form disrupted autophagy, but did not impede proliferation. Further characterisation showed bloodstream form autophagy mutants retain the capacity to undergo the complex cellular remodelling that occurs during differentiation to the procyclic form and are equally susceptible to dihydroxyacetone-induced cell death as wild type parasites, not supporting a role for autophagy in this cell death mechanism. The RNAi reporter system developed, which also identified TOR1 as a negative regulator controlling YFP-ATG8.2 but not YFP-ATG8.1 autophagosome formation, will enable further targeted analysis of the mechanisms and function of autophagy in the medically relevant bloodstream form of T. brucei
ALMA CO J=6-5 observations of IRAS16293-2422: Shocks and entrainment
Observations of higher-excited transitions of abundant molecules such as CO
are important for determining where energy in the form of shocks is fed back
into the parental envelope of forming stars. The nearby prototypical and
protobinary low-mass hot core, IRAS16293-2422 (I16293) is ideal for such a
study. The source was targeted with ALMA for science verification purposes in
band 9, which includes CO J=6-5 (E_up/k_B ~ 116 K), at an unprecedented spatial
resolution (~0.2", 25 AU). I16293 itself is composed of two sources, A and B,
with a projected distance of 5". CO J=6-5 emission is detected throughout the
region, particularly in small, arcsecond-sized hotspots, where the outflow
interacts with the envelope. The observations only recover a fraction of the
emission in the line wings when compared to data from single-dish telescopes,
with a higher fraction of emission recovered at higher velocities. The very
high angular resolution of these new data reveal that a bow shock from source A
coincides, in the plane of the sky, with the position of source B. Source B, on
the other hand, does not show current outflow activity. In this region, outflow
entrainment takes place over large spatial scales, >~ 100 AU, and in small
discrete knots. This unique dataset shows that the combination of a
high-temperature tracer (e.g., CO J=6-5) and very high angular resolution
observations is crucial for interpreting the structure of the warm inner
environment of low-mass protostars.Comment: Accepted for publication in A&A Letter
The RMS Survey: Resolving kinematic distance ambiguities towards a sample of compact HII regions using HI absorption
We present high-resolution HI data obtained using the Australia Telescope
Compact Array to resolve the near/far distance ambiguities towards a sample of
compact HII regions from the Red MSX Source (RMS) survey. The high resolution
data are complemented with lower resolution archival HI data extracted from the
Southern and VLA Galactic Plane surveys. We resolve the distance ambiguity for
nearly all of the 105 sources where the continuum was strong enough to allow
analysis of the HI absorption line structure. This represents another step in
the determination of distances to the total RMS sample, which with over 1,000
massive young stellar objects and compact HII regions, is the largest and most
complete sample of its kind. The full sample will allow the distribution of
massive star formation in the Galaxy to be examined.Comment: Accepted by MNRAS. This paper consists of 15 pages and contains 10
figures and 5 table
The RMS Survey: Far-Infrared Photometry of Young Massive Stars
Context: The Red MSX Source (RMS) survey is a multi-wavelength campaign of
follow-up observations of a colour-selected sample of candidate massive young
stellar objects (MYSOs) in the galactic plane. This survey is returning the
largest well-selected sample of MYSOs to date, while identifying other dust
contaminant sources with similar mid-infrared colours including a large number
of new ultra-compact (UC)HII regions. Aims:To measure the far-infrared (IR)
flux, which lies near the peak of the spectral energy distribution (SED) of
MYSOs and UCHII regions, so that, together with distance information, the
luminosity of these sources can be obtained. Methods:Less than 50% of RMS
sources are associated with IRAS point sources with detections at 60 micron and
100 micron, though the vast majority are visible in Spitzer MIPSGAL or IRAS
Galaxy Atlas (IGA) images. However, standard aperture photometry is not
appropriate for these data due to crowding of sources and strong spatially
variable far-IR background emission in the galactic plane. A new technique
using a 2-dimensional fit to the background in an annulus around each source is
therefore used to obtain far-IR photometry for young RMS sources.
Results:Far-IR fluxes are obtained for a total of 1113 RMS candidates
identified as young sources. Of these 734 have flux measurements using IGA 60
micron and 100 micron images and 724 using MIPSGAL 70 micron images, with 345
having measurements in both data sets.Comment: 10 pages, 10 figures, 2 Tables, accepted to A&A. A full version of
table 1 is available from the lead author or at the CDS upon publicatio
Dose-Dependent Response to Cyclodextrin Infusion in a Rat Model of Verapamil Toxicity
Introduction: Sulfobutylether-b-cyclodextrin (SBE-CD) is a pharmaceutical excipient known to bind verapamil. Following intravenous administration, clearance of SBE-CD approximates glomerular filtration rate. We hypothesized that infusion of SBE-CD would increase time to asystole in a rat model of verapamil toxicity in a dose-dependent manner. The objective was to demonstrate the effect of a range of SBE-CD concentrations in a rat model of verapamil toxicity. Methods: Twenty-five Wistar rats were allocated to control or 1 of 4 intervention groups. All received ketamine and diazepam anesthesia followed by verapamil infusion 32 mg/kg/h. The verapamil infusion for the intervention groups was premixed with SBE-CD in a 1:1, 1:2, 1:4, or 1:8 molar ratio (verapamil to SBE-CD). The control group infusion did not contain SBE-CD. Additional saline or water was added to the infusion so that the total volume infused was the same across groups, and the osmolality was maintained as close to physiologic as possible. Heart rate, respiratory rate, and temperature were monitored. The primary endpoint was time to asystole.Results: Verapamil coinfused with SBE-CD in a molar ratio of 1:4 resulted in prolonged time to asystole compared to control (21.2 minutes vs 17.6 minutes, P , 0.05). There were no differences in time to asystole between control and any other intervention group. There was no significant difference in time to apnea between control and any intervention group. We assessed the effect of a range of SBE-CD concentrations and identified 1 concentration that prolonged time to asystole. Mechanismsthat may explain this effect include optimal volume expansion with a hyperosmolar cyclodextrin containing solution, complexation of verapamil within the hydrophobic cyclodextrin pore, and/or complexation within micelle-like aggregates of cyclodextrin. However, mechanistic explanations for the observed findings are speculative at this point. Conclusion: The 1:4 verapamil to SBE-CD concentration was modestly effective with SBE-CD concentrations above and below this range demonstrating nonstatistically significant improvements in time to asystole. [West J Emerg Med. 2012;13(1):63–67.
VLTI/MIDI 10 micron interferometry of the forming massive star W33A
We report on resolved interferometric observations with VLTI/MIDI of the
massive young stellar object (MYSO) W33A. The MIDI observations deliver
spectrally dispersed visibilities with values between 0.03 and 0.06, for a
baseline of 45m over the wavelength range 8-13 micron. The visibilities
indicate that W33A has a FWHM size of approximately 120AU (0.030'') at 8 micron
which increases to 240AU at 13 micron, scales previously unexplored among
MYSOs. This observed trend is consistent with the temperature falling off with
distance. 1D dust radiative transfer models are simultaneously fit to the
visibility spectrum, the strong silicate feature and the shape of the mid
infrared spectral energy distribution (SED). For any powerlaw density
distribution, we find that the sizes (as implied by the visibilities) and the
stellar luminosity are incompatible. A reduction to a third of W33A's
previously adopted luminosity is required to match the visibilities; such a
reduction is consistent with new high resolution 70 micron data from Spitzer's
MIPSGAL survey. We obtain best fits for models with shallow dust density
distributions of r^(-0.5) and r^(-1.0) and for increased optical depth in the
silicate feature produced by decreasing the ISM ratio of graphite to silicates
and using optical grain properties by Ossenkopf et al. (1992).Comment: 4 pages, 4 figures. Accepted for ApJ letter
The RMS Survey: The Bolometric Fluxes and Luminosity Distributions of Young Massive Stars
Context: The Red MSX Source (RMS) survey is returning a large sample of
massive young stellar objects (MYSOs) and ultra-compact (UC) \HII{} regions
using follow-up observations of colour-selected candidates from the MSX point
source catalogue. Aims: To obtain the bolometric fluxes and, using kinematic
distance information, the luminosities for young RMS sources with far-infrared
fluxes. Methods: We use a model spectral energy distribution (SED) fitter to
obtain the bolometric flux for our sources, given flux data from our work and
the literature. The inputs to the model fitter were optimised by a series of
investigations designed to reveal the effect varying these inputs had on the
resulting bolometric flux. Kinematic distances derived from molecular line
observations were then used to calculate the luminosity of each source.
Results: Bolometric fluxes are obtained for 1173 young RMS sources, of which
1069 have uniquely constrained kinematic distances and good SED fits. A
comparison of the bolometric fluxes obtained using SED fitting with trapezium
rule integration and two component greybody fits was also undertaken, and
showed that both produce considerable scatter compared to the method used here.
Conclusions: The bolometric flux results allowed us to obtain the luminosity
distributions of YSOs and UC\HII{} regions in the RMS sample, which we find to
be different. We also find that there are few MYSOs with L
10\lsol{}, despite finding many MYSOs with 10\lsol{} L
10\lsol{}.Comment: 12 pages, 12 figures, 3 tables, accepted to A&A. The full versions of
tables 1 and 2 will be available via the CDS upon publicatio
The RMS Survey: 6 cm continuum VLA observations towards candidate massive YSOs in the northern hemisphere
(Abridged) Context: The Red MSX Source (RMS) survey is an ongoing
multi-wavelength observational programme designed to return a large,
well-selected sample of massive young stellar objects (MYSOs). We have
identified 2000 MYSO candidates located throughout the Galaxy by
comparing the colours of MSX and 2MASS point sources to those of known MYSOs.
Aims: To identify the populations of UCHII regions and PNe within the sample
and examine their Galactic distribution. Method: We have conducted high
resolution radio continuum observations at 6 cm towards 659 MYSO candidates in
the northern hemisphere (10\degr< l < 250\degr) using the VLA. In addition to
these targeted observations we present archival data towards a further 315 RMS
sources extracted from a previous VLA survey of the inner Galaxy. Results: We
find radio emission towards 272 (27% of the observed sample). Using
results from other parts of our multi-wavelength survey we separate these
RMS-radio associations into two distinct types of objects, classifying 51 as
PNe and a further 208 as either compact or UC HII regions. Using this well
selected sample of HII regions we estimate their Galactic scale height to be
0.6\degr. Conclusions: Using radio continuum and archival data we have
identified 79 PNe and 391 HII regions within the northern RMS catalogue. We
estimate the total fraction of contamination by PNe in the RMS sample is of
order 10%. The sample of HII regions is probably the best representation to
date of the Galactic population of HII regions as a whole.Comment: Accepted for publication in Astronomy and Astrophysics. 15 pages, 9
figures and 5 tables. Full versions of Tables 3, 4 and 5 and Figs. 2, 4 and 7
will only be available via CDS or the RMS website at
http:/www.ast.leeds.ac.uk/cgi-bin/RMS/RMS_VLA_IMAGES.cg
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