The Vehicle Routing Problem with Divisible Deliveries and Pickups

The vehicle routing problem with divisible deliveries and pickups is a new and interesting model within reverse logistics. Each customer may have a pickup and delivery demand that have to be served with capacitated vehicles. The pickup and the delivery quantities may be served, if beneficial, in two separate visits. The model is placed in the context of other delivery and pickup problems and formulated as a mixed-integer linear programming problem. In this paper, we study the savings that can be achieved by allowing the pickup and delivery quantities to be served separately with respect to the case where the quantities have to be served simultaneously. Both exact and heuristic results are analysed in depth for a better understanding of the problem structure and an average estimation of the savings due to the possibility of serving pickup and delivery quantities separately

Structure and electronic properties of the quasi-one-dimensional Ba₂Co₁₋ₓZnₓS₃ series

This work focuses on the structure and physical properties of the solid solution Ba₂Co₁₋ₓZnₓS₃ (0 ≤ x ≤ 1), a family of quasi-one-dimensional sulfides with end members Ba₂CoS₃ and Ba₂ZnS₃. The structure of selected compounds with increasing Zn²⁺ content has been analysed using, neutron diffraction, TEM and EXAFS and the physical properties via magnetic susceptibility and resistivity measurements. The progressive substitution of the non-magnetic Zn²⁺ cation for Co²⁺ rapidly destroys the antiferromagnetic transition present at 46 K in the quasi one-dimensional Ba₂CoS₃, leading to paramagnetic behaviour down to the lowest investigated temperature (5K) for compounds with x > 0.25. For compounds with x ≥ 0.4, a pure CW regime is recovered around 300 K, yielding effective moments consistent with the g factor of the tetrahedrally coordinated Co²⁺ previously determined for Ba₂CoS₃. The Zn²⁺/Co²⁺ substitution also removes the metallic-like behaviour of Ba₂CoS₃ causing an increase in the value of the resistivity with all the Ba₂Co₁₋ₓZnₓS₃ compounds showing semiconducting behaviour. The negative magnetoresistance of Ba₂CoS₃ is improved by the Zn²⁺/Co²⁺ substitution, with values of – 6% for Ba₂Co₀.₇₅Zn₀.₂₅S₃, – 9% for Ba₂Co₀.₅Zn₀.₅S₃ and – 8% for Ba₂Co₀.₂₅Zn₀.₇₅S₃. However, there does not seem to be a correlation between the values of the resistivity and the magnetoresistance and the content of Zn²⁺, leading to the hypothesis that transport properties may be linked more closely to extrinsic properties

Analysis of the air pollution climate at a background site in the Po valley

The Po valley in northern Italy is renowned for its high air pollutant concentrations. Measurements of air pollutants from a background site in Modena, a town of 200 thousand inhabitants within the Po valley, are analysed. These comprise hourly data for CO, NO, NO2, NOx, and O3, and daily gravimetric equivalent data for PM10 from 1998\u20132010. The data are analysed in terms of long-term trends, annual, weekly and diurnal cycles, and auto-correlation and cross-correlation functions. CO, NO and NO2 exhibit a strongly traffic-related pattern, with daily peaks at morning and evening rush hour and lower concentrations over the weekend. Ozone shows an annual cycle with a peak in July due to local production; notwithstanding the diurnal cycle dominated by titration by nitrogen oxide, the decreasing long term trend in NO concentration did not affect the long term trend in O3, whose mean concentration remained steady over the sampling period. PM10 shows a strong seasonality with higher concentration in winter and lower concentration in summer and spring. Both PM10 and ozone show a marked weekly cycle in summer and winter respectively. Regressions of PM10 upon NOx show a consistently greater intercept in winter, representing higher secondary PM10 in the cooler months of the year. There is a seasonal pattern in primary PM10 to NOx ratios, with lower values in winter and higher values in summer, but the reasons are unclear

Remarks on some tardigrades of the African fauna with the description of three new species of Macrobiotus Schultze 1834

Ten species of tardigrades are recorded. Three of them, Macrobiotus radiatus, Macrobiotus vanescens, and Macrobiotus iharosi are new for science; two species, Macrobiotus sapiens Binda & Pilato 1984 and Isohypsibius kristenseni Pilato et al. 1989, are new for the African fauna. Macrobiotus radiatus n. sp. differs from the other species of the harmsioorthi group in the characters of the eggs and in other characters regarding various structures (buccal armature, or placoids length, or the insertion point of the stylet supports, or the claws length). Macrobiotus vanescens n. sp. is similar to Macrobiotus richtersi Murray 1911 and to Macrobiotus peteri Pilato et al. 1989 but differs from them in some characters regarding the bucco-pharyngeal apparatus, the claws and the eggs. Macrobiotus iharosi n. sp. differs from Macrobiotus echinogenitus Richters 1904 in the characters of the eggs, and from Macrobiotus sapiens Binda & Pilato 1984 in some characters of the bucco-pharyngeal apparatus and of the claws

Multiphoton Absorption of Myoglobin–Nitric Oxide Complex: Relaxation by D-NEMD of a Stationary State

ABSTRACT: The photodissociation and geminate recombination of nitric oxide in myoglobin, under continuous illumination, is modeled computationally. The relaxation of the photon energy into the protein matrix is also considered in a single simulation scheme that mimics a complete experimental setup. The dynamic approach to non-equilibrium molecular dynamics is used, starting from a steady state, to compute its relaxation to equilibrium. Simulations are conducted for the native form of sperm whale myoglobin and for two other mutants, V68W and L29F, illustrating a fair diversity of spatial and temporal geminate recombination processes. Energy flow to the heme and immediate protein environment provide hints to allostery. In particular, a pathway of energy flow between the heme and the FG loop is illustrated. Although the simulations were conducted for myoglobin only, the thermal fluctuations of the FG corner are in agreement with the large structural shifts of FG during the allosteric transition of tetrameric hemoglobin

Detection of Ligand-induced Conformational Changes in the Activation Loop of Aurora-A Kinase by PELDOR Spectroscopy.

The structure of protein kinases has been extensively studied by protein crystallography. Conformational movement of the kinase activation loop is thought to be crucial for regulation of activity; however, in many cases the position of the activation loop in solution is unknown. Protein kinases are an important class of therapeutic target and kinase inhibitors are classified by their effect on the activation loop. Here, we report the use of pulsed electron double resonance (PELDOR) and site-directed spin labeling to monitor conformational changes through the insertion of MTSL [S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1 H-pyrrol-3-yl)methyl methanesulfonothioate] on the dynamic activation loop and a stable site on the outer surface of the enzyme. The action of different ligands such as microtubule-associated protein (TPX2) and inhibitors could be discriminated as well as their ability to lock the activation loop in a fixed conformation. This study provides evidence for structural adaptations that could be used for drug design and a methodological approach that has potential to characterize inhibitors in development

Growth performance and oxidative status in piglets supplemented with verbascoside and teupolioside

Two hundred forty piglets, half female and half barrows, 8.1 ± 1.40 kg LW, were divided into 6 experimental groups and fed ad libitum with a diet supplemented with the following levels of antioxidants: 0 (CON + = positive control added with 100 mg lincomicine/kg), 5 (LT = low teupolioside or LV = low verbascoside), 10 (HT = high teupolioside; HV = high verbascoside; LT+LV) mg/kg of diet for 56 days. Body weight and feed intake were recorded on d0, 14 and 56 of the trial. Ten piglets from each group were selected and blood collected by anterior vena cava puncture at 0, 14 and 56 d for reactive oxygen metabolite (ROMs) determination. HV showed final weight higher than the other groups (P<0.05), and oxidative stability was improved by both integrations of verbascoside. These results support the view that Verbascoside influences the growth performances and oxidative status of piglets