Development and characterization of new microsatellite markers for grape

Thirty five new grape microsatellite markers were developed under an international consortium involving AGROGENE. These loci were amplified in 41 Spanish cultivars of V. vinifera. Eleven of the markers were polymorphic and informative in V. vinifera. Twelve were monomorphic and of the remaining markers one was polymorphic but less useful because individuals amplified more than two bands and the rest had amplification problems. The number of alleles detected for the 11 informative markers ranged from 4 to 12, with heterozygosity values ranging from 0.6 to 0.8. Primer sequences are reported for these markers.

Molecular characterization of table grape varieties preserved in the Rancho de la Merced Grapevine Germplasm Bank (Spain)

A collection of 317 table-grape accessions preserved in the Vitis Germplasm Bank at the Rancho de la Merced (IFAPA, Jerez de la Frontera, Spain) were genotyped at 20 microsatellite loci. A total of 264 different genotypes were obtained. Among them, 66 are presented for the first time. Twenty-one accession names are considered new synonyms, fourteen homonyms and 48 can be considered as "denomination mistakes" in the Rancho de la Merced collection

On the absence of acylated anthocyanins in some wild grapevine accessions

Our current research is focused on the anthocyanin composition of female grape accessions, mostly Spanish, preserved at El Encín Germplasm Bank. In 2008, berries of 126 accessions were taken at maturity. After the extraction from grape skins, total anthocyans were determined by spectrophotometry, and the anthocyanin fingerprint of grapes by HPLC, considering the relative amount of 15 anthocyanins. Among those 126 accessions, 23 genotypes (18.25 %) did not present acylated anthocyanins. Thus, those 23 genotypes presented a characteristic anthocyanin fingerprint, similar at a certain extent to that presented by some Rhine basin and Italian grape cultivars, e.g., 'Pinot Noir' and 'Gaglioppo'. Nevertheless, the absence of acylated anthocyanins has not been described in any Spanish grape cultivar. The examination of the anthocyanin fingerprint of wild grapes without acylated anthocyanins reveals that the regulation of the anthocyanin biosynthesis may differ in various wild grape accessions.

Correlación clínica e inmunohistopatológica de la nefropatía lúpica en un centro de referencia del Caribe colombiano durante los años 2012 a 2013

Objectives: To determine the behavior of lupus nephropathy (LN) in a population group of a reference center in the Colombian Caribbean during 2012 and 2013, analyzing the most prevalent clinical and laboratory findings and its relation to the immunohistotopathological findings, define the class of Lupus nephritis with greater prevalence and the clinical or paraclinic data more correlated with this one. Methods: Descriptive, retrospective, cross-sectional study conducted from January 1st of 2012 to December 31st 2013, which included 53 patients with systemic lupus erythematosus according to diagnostic critearia of the Colegio Americano de Reumatologia, with manifestations of renal impairment. Results: In our study population in the Colombian Caribbean, LN predominates more in women and class IV represents 66.03% of the total cases analyzed, in which there was more hematuria present, hypertension and proteinuria with nephrotic pattern, as well as higher levels of serum creatinine. Despite the lack of data, none of the clinical or paraclinical variables was specific for a certain class of LN and in relation to the immunostaining, lambda chains were positive in 100% of the cases and only C4 and fibrinogen were specific to LN class IV. Conclusions: Class IV LN is the most prevalent and with a higher percentage of predominance of the variables hematuria, proteinuria, hypertension, elevated serum creatinine and bun levels. At the same time they are not specific for some class of LN in our population.Objetivos: Determinar el comportamiento de la nefropatía lúpica (NL) en un grupo poblacional de un centro de referencia del Caribe colombiano, durante los años 2012 y 2013, analizando los hallazgos clínicos  de laboratorio más prevalentes y su relación con los hallazgos inmunohistopatológicos, definir la clase de NL con mayor prevalencia y el dato clínico o paraclínico más correlacionado con esta.Métodos: Estudio descriptivo, retrospectivo, transversal, realizado desde el 1 de enero de 2012 al 31 de diciembre de 2013, que incluyó a 53 pacientes con lupus eritematoso sistémico (LES), según los criterios diagnósticos del Colegio Americano de Reumatología, con manifestaciones de compromiso renal.Resultados: En nuestra población de estudio del Caribe colombiano, la NL predomina más en mujeres y la clase IV representa el 66,03% del total de casos analizados, en los cuales hubo más presentación de hematuria, hipertensión y proteinuria con patrón nefrótico, al igual que mayores niveles de creatinina sérica. A pesar de la falta de datos, ninguna de las variables clínicas o paraclínicas fue específica para cierta clase de NL y con relación a la inmunomarcación, las cadenas lambda se presentaron positivas en el 100% de los casos y tan solo el C4 y el fibrinógeno fueron específicos de la NL clase IV.Conclusiones: La NL clase IV es la más prevalente y con mayor porcentaje de predominio de las variables como hematuria, proteinuria, hipertensión, niveles de creatinina y BUN séricos elevados y, a su vez, no son específicos para cierta clase de NL en nuestra población

Design of a Trans-Horizon radio link for ultra high and super high frequencies

Cost considerations and availability of frequencies condition the feasibility of links at distances in which there is no line of sight between antennas. These trans-horizon links require precise design procedures to determine whether the intensity of the signal and its noise ratio allow reception with reasonable quality. The present work describes a procedure for radio electric link calculation to transmit voice and data signals through bandwidth at ultra high and super high frequencies.Presentado en el V Workshop Arquitectura, Redes y Sistemas Operativos (WARSO)Red de Universidades con Carreras en Informática (RedUNCI

Conservation of the Spanish Alano dog breed: phaneroptical characterisation

A preliminary phaneroptical characterisation of the Spanish Alano Dog Breed is done studying, on a random sample formed by 135 specimens, six external variables: coat colour and distribution; hair type; mucosae, and iris colour; type of bite; and absence of premolars. We have concluded that this breed is characterised by the presentation of yellow and brindled coats, with other coat colours as residuals, standing out the combined black and brindled coat, not present in other related breeds, what could be considered as a breed marker character. The breed is also characterised by short hair, black pigmented mucosae , brown iris, with general presentation of a mask. The dentition characteristics are the presence of a prognatic bite (slight) and absence of premolars, both characters related to the origin of the breed.Se realiza una caracterización faneróptica preliminar sobre una muestra aleatoria de 135 ejemplares de raza Alano Español. Se consideran las variables: color y distribución de la capa, tipo de pelo, coloración de las mucosas e iris, tipo de mordida y ausencia de premolares. Se concluye que el Alano Español presenta como capas predominantes la barcina y la leonada, siendo minoritarias la capa negro y bardino, ausente en la mayoría de razas consultadas, y que podría constituirse como un carácter marcador de raza. Asimismo la raza se caracteriza por presentar pelo liso, mucosas pigmentadas en negro, iris de color castaño, con presencia de máscara generalmente. La raza mayoritariamente presenta una mordida prognática (tijera invertida), con ausencia de premolares, caracteres ambos acordes con el origen de la raza

Temperature dependence of the magnetic properties in LaMnO_(3+δ)

Data are presented on the thermal dependence of the hysteretic properties of cationic vacancies including manganite samples of composition LaMnO_(3+δ)(δ=0.05 and 0.12). Our results evidence the presence in both samples of two magnetic phases having ferro- and antiferromagnetic orders, respectively. The temperature dependence of the coercivity and relaxational properties of the samples is closely linked to the connectivity of the magnetic moment bearing Mn^(3+)-Mn^(4+) ferromagnetic clusters that demagnetize independently in the case of the δ=0.05 sample and collectively in that of the δ=0.12 one, as evidenced from the activation volume results (delta=0.05) which yielded a size of the same order magnitude as that obtained in previous works for the Mn^(3+)-Mn^(4+) ferromagnetic cluster size

Long-Term Adherence to IFN Beta-1a Treatment when Using RebiSmart® Device in Patients with Relapsing-Remitting Multiple Sclerosis

The effectiveness of disease-modifying drugs in the treatment of multiple sclerosis is associated with adherence. RebiSmart (R) electronic device provides useful information about adherence to the treatment with subcutaneous (sc) interferon (IFN) beta-1 alpha (Rebif (R)). The aim of the study was to determine long-term adherence to this treatment in patients with relapsing-remitting multiple sclerosis (RRMS). This retrospective multicentre observational study analysed 258 patients with RRMS who were receiving sc IFN beta-1 alpha (Rebif (R)) treatment by using RebiSmart (R) until replacement (36 months maximum lifetime) or treatment discontinuation. Adherence was calculated with data (injection dosage, time, and date) automatically recorded by RebiSmart (R). Patients in the study had a mean age of 41 years with a female proportion of 68%. Mean EDSS score at start of treatment was 1.8 (95% CI, 1.6-1.9). Overall adherence was 92.6%(95% CI, 90.6-94.5%). A total of 30.2% of patients achieved an adherence rate of 100%, 80.6% at least 90%, and only 13.2% of patients showed a suboptimal adherence (<80%). A total of 59.9% of subjects were relapse-free after treatment initiation. Among 106 subjects (41.1%) who experienced, on average, 1.4 relapses, the majority were mild (40.6%) or moderate (47.2%). Having experienced relapses from the beginning of the treatment was the only variable significantly related to achieving an adherence of at least 80% (OR = 3.06, 1.28-7.31). Results of this study indicate that sc IFN beta-1 alpha administration facilitated by RebiSmart (R) could lead to high rates of adherence to a prescribed dose regimen over 36 months

Application of molecular topology to the prediction of inhibition of Trypanosoma cruzi Hexokinase by bisphosphonates

Se ha desarrollado un modelo topológico-matemático para la búsqueda de nuevos derivados bisfosfonatos activos frente a la hexokinasa de Trypanosoma cruzi. Utilizando el análisis lineal discriminante se ha seleccionado una función con cuatro variables capaz de predecir adecuadamente la CI50 para cada compuesto de las series de entrenamiento y test. El modelo propuesto se ha aplicado a una librería molecular y se han propuesto nuevas estructuras potencialmente activas frente a T. cruzi.A topological-mathematical model has been arranged to search for new derivatives of bisphosphonate compounds acting as inhibitors against Trypanosoma cruzi hexokinase. By using linear discriminant analysis, a four-variable function was achieved allowing an accurate prediction of the IC50 for each compound of the training and test series. After carrying out a virtual screening based upon such a model, new structures potentially actives against T. cruzi are proposed.Este trabajo ha sido posible gracias al Fondo de Investigación Sanitaria, Ministerio de Sanidad, España (proyecto SAF2005-PIO52128) y al Máster Internacional en Enfermedades Parasitarias Tropicales, Universidad de Valencia

Aplicación de la topología molecular en la predicción de la inhibición de Trypanosoma cruzi Hexokinasa y un grupo de derivados bifosfonatos

A topological-mathematical model has been arranged to search for new derivatives of bisphosphonate compounds actingas inhibitors against Trypanosoma cruzi hexokinase. By using linear discriminant analysis, a four-variable function wasachieved allowing an accurate prediction of the IC50 for each compound of the training and test series. After carryingout a virtual screening based upon such a model, new structures potentially actives against T. cruzi are proposedSe ha desarrollado un modelo topológico-matemático para la búsqueda de nuevos derivados bisfosfonatos activosfrente a la hexokinasa de Trypanosoma cruzi. Utilizando el análisis lineal discriminante se ha seleccionado una funcióncon cuatro variables capaz de predecir adecuadamente la CI50 para cada compuesto de las series de entrenamientoy test. El modelo propuesto se ha aplicado a una librería molecular y se han propuesto nuevas estructuraspotencialmente activas frente a T. cruzi