Sensor Systems for Impaired Healing Markers, Concepts and Applications for Objective Wound Assessment

In the pathological healing of chronic wounds the ordered sequence of tissue restoration is disturbed. As a consequence, chronic wounds fail to heal within months and pose a major impact on the patient by pain, odor, leakage, and the risk of infection and the health care system by its constant treatment. Introducing objective wound assessment by sensor technology into clinical routine would help to guide treatment procedures and improve the healing outcome. The aim of this research study was the development of simple and effective concepts to evaluate the wound status at the point of care. Requirements for point of care testing include a fast and flexible device use by untrained personal, reduced time and costs, as well as reliable and easy to interpret results. The complex nature of wounds can be divided into the different regimes of physical appearance, biochemical status and microbiological environment, which influence each other. Each regime is tackled separately in this work by identifying possible parameters for wound analysis from the literature and the design of sensor concepts to quantify these candidates. A miniaturized, wearable sensor system for the integration in a wound dressing was developed to collect healing relevant, physiological data. The sensor measures optical reflectance, heart rate, arterial oxigenation, surface pH, moisture and temperature. The function of the sensor system was verified in a porcine wound model. A combination of surface pH, reflected infrared, and red light showed to be the most significant parameter, associated with the healing progress. For the measurement of biochemical parameters a microfluidic platform for the preparation of biosensing hydrogels by in situ polymerization was designed. Introducing functional structures for gel patterning in the chip fabrication allows for rapid assay customization. Simple handling and functionality were illustrated by assays for matrix metalloproteinase, an important factor in chronic wound healing. In addition, the demonstrated assays for total protein concentration and cell counts indicate the possibilities for a wide range of fast and simple diagnostics. The last part of the thesis discusses microfluidic technologies for rapid analysis of bacteria. Preconcentration of bacteria by on-chip electrophoresis and detection by a simple optical setup are presented. Furthermore, devices for rapid and parallel growth-based bacterial identification and antibiotic testing in microfluidic cultures were designed. The presented results and demonstrated tools show that medical analysis can be improved by sensor technologies that are simple to operate and yield fast results at the point of care

Creating ‘Heimat’ in the City: ‘Volkskultur’ in the Viennese Interwar Period

The article focuses on the signi cance of Vienna, Austria’s capital and only major city, for the creation and development of ‘Volkskultur’ (folk culture) during the interwar period. It examines ‘Volkskultur’ as a modern concept to cope with times of crisis. The contribution locates this culture in urban processes, dynamics and discourses on ‘Heimat’. It presents ‘Volkskultur’ as an influential large-scale urban popular culture and Vienna as the central and biggest arena of folk cultural formats and practices in Austria.The article focuses on the signi cance of Vienna, Austria’s capital and only major city, for the creation and development of ‘Volkskultur’ (folk culture) during the interwar period. It examines ‘Volkskultur’ as a modern concept to cope with times of crisis. The contribution locates this culture in urban processes, dynamics and discourses on ‘Heimat’. It presents ‘Volkskultur’ as an influential large-scale urban popular culture and Vienna as the central and biggest arena of folk cultural formats and practices in Austria

Hubert Puchberger: Nijema snaga znaka

Prijevod teksta o značenju i važnosti riječi u liturgiji nakon II. vatikanskog sabora

The noise of many needles: Jerky domain wall propagation in PbZrO3 and LaAlO3

Measurements of the sample length of PbZrO3 and LaAlO3 under slowly increasing force (3-30 mN/min) yield a superposition of a continuous decrease interrupted by discontinuous drops. This strain intermittency is induced by the jerky movement of ferroelastic domain walls through avalanches near the depinning threshold. At temperatures close to the domain freezing regime, the distributions of the calculated squared drop velocity maxima N(υm2) follow a power law behaviour with exponents ε=1.6±0.2. This is in good agreement with the energy exponent ε=1.8±0.2 recently found for the movement of a single needle tip in LaAlO3 [R. J. Harrison and E. K. H. Salje, Appl. Phys. Lett. 97, 021907 (2010)]. With increasing temperature, N(υm2) changes from a power law at low temperatures to an exponential law at elevated temperatures, indicating that thermal fluctuations increasingly enable domain wall segments to unpin even when the driving force is smaller than the corresponding barrier

Cell Separation in a Continuous Flow by Traveling Wave Dielectrophoresis

In this contribution we present a microfluidic chip for the continuous and label-free separation of cells. Strip electrodes produce a traveling electric field perpendicular to the pressure driven flow. Viable cells are deflected parallel to the field by traveling wave dielectrophoresis (twDEP) according to their volume and dielectric properties. With the present device we have successfully separated viable Saccharomyces cerevisiae and Jurkat T-cells from debris, non-viable cells and Lactobacillus casei

Технологическое обеспечение радиохирургии метастазов в головном мозге

Расчёт поглощенных доз с помощью системы планирования для реализации стереотаксической лучевой терапии на базе линейного ускорителя, сравнение лечебных планов с разными режимами доставки дозыCalculation of the absorbed dose using the planning system for the implementation linear accelerator-based stereotactic radiation therapy, comparison of medical plans with different modes of delivery of the dos

Access to Organometallic Arylcobaltcorrins through Radical Synthesis: 4‐Ethylphenylcobalamin, a Potential “Antivitamin B 12 ”

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96761/1/anie_201209651_sm_miscellaneous_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/96761/2/2606_ftp.pd

Zugang zu metallorganischen Arylcobaltcorrinen durch radikalische Synthese: 4‐Ethylphenylcobalamin, ein potenzielles “Antivitamin B 12 ”

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/96656/1/2668_ftp.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/96656/2/ange_201209651_sm_miscellaneous_information.pd

Organophosphonate bridged anatase mesocrystals: low temperature crystallization, thermal growth and hydrogen photo-evolution

The sol-gel co-condensation of organo-phosphonates to titanium alkoxides enables access to novel organic-inorganic hybrids based on phosphonate-bridged titanium dioxide. In this contribution, we bring new perspectives to the long established sol-gel mineralization of titanium alkoxide species, by harnessing the virtues of the well-designed phosphonate-terminated phosphorus dendrimers as reactive amphiphilic nanoreactor, confined medium and cross-linked template to generate discrete crystalline anatase nanoparticles at low temperature (T = 60 degrees C). An accurate investigation on several parameters (dendrimer generation, dendrimer-to-titanium alkoxide ratio, precursor reactivity, temperature, solvent nature, salt effect) allows a correlation between the network condensation, the opening porous framework and the crystalline phase formation. The evolution of the dendrimer skeleton upon heat treatment has been deeply monitored by means of P-31 NMR, XPS and Raman spectroscopy. Increasing the heteroatom content within a titania network provides the driving force for enhancing their photocatalytic water splitting ability for hydrogen production.Brahmi, Y.; Katir, N.; Macia Agullo, JA.; Primo Arnau, AM.; Bousmina, M.; Majoral, J.; García Gómez, H.... (2015). Organophosphonate bridged anatase mesocrystals: low temperature crystallization, thermal growth and hydrogen photo-evolution. Dalton Transactions. 44(35):15544-15556. doi:10.1039/c5dt02367jS1554415556443