The Virtual Museum of Zoology of the University of Granada and the acquisition of practical knowledge by students

Durante los cursos académicos 2008-2012, en el Departamento de Zoología de la Universidad de Granada (UGR), se implantó una nueva herramienta didáctica: se llevó a cabo la creación de un Museo Virtual de Zoología, que consistió en la digitalización del material para prácticas ya existente en el citado Departamento, perteneciente a una colección histórica de animales naturalizados y conservados. El material digitalizado incluyó tanto imágenes de animales de la colección, como al natural, así como información básica de los mismos (p. ej.: encuadre taxonómico, hábitats y modos de vida). Esta información se puso a disposición de los alumnos de las asignaturas de Zoología, tanto de Ciencias Ambientales como de Biología, a través de una página Web gratuita. Este museo complementa la colección de animales existente previamente en el Departamento, y que también está a disposición de los alumnos para su consulta. Para conocer la efectividad de esta nueva herramienta, hemos encuestado al profesorado de varias universidades españolas y al alumnado de la Universidad de Granada sobre el grado de utilización e idoneidad del museo, y comparado los porcentajes de alumnos presentados al examen de prácticas y resultados de las notas entre el último curso sin museo (2008-2009) y el primero que lo tuvo a su disposición (2011-2012). Presentamos los resultados en este estudio. El Museo Virtual de Zoología es gratuito y está disposición de cualquier Centro/personal docente que quiera hacer uso del mismo, además del público en general. Ponemos de manifiesto la importancia de la creación de herramientas virtuales de este tipo para la mejora de la docencia práctica en las asignaturas sobre Historia Natural de los seres vivos.The Department of Zoology of the University of Granada (UGR) introduced a new educational tool between 2008-2012: the Virtual Museum of Zoology. This tool was created mainly with the digitalization of the historical collection of naturalized and preserved animals of the department. The tool also included images of animals in their natural habitats and basic information (e.g. taxonomical classification, habitat and feeding habits). This information was included in a free webpage for its use by the students of Zoology of Environmental Sciences and Biology Degrees. To evaluate the effectiveness of this new tool, we poll for the opinion of Zoology teachers of Spanish universities and for the level of utilization and suitability of the museum by Zoology students of the University of Granada. In addition, we compared the percentage of students presented to the tests and their results between the previous and subsequent year to the introduction of this tool. We present these results in this study. The Virtual Museum of Zoology is free to use by any student, teacher or institution. We highlight the importance of these virtual tools for the improvement of Natural History practical teaching

Evaluación de la calidad ambiental de ecosistemas acuáticos en el entorno del Parque Nacional de Doñana

Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 201

The role of selenium in shaping mice brain metabolome and selenoproteome through the gut-brain axis by combining metabolomics, metallomics, gene expression, and amplicon sequencing

Selenium (Se) is a trace element crucial for human health. Recently, the impact of Se supplementation on gut microbiota has been pointed out as well as its influence on the expression of certain selenoproteins and gut metabolites. This study aims to elucidate the link between Se supplementation, brain selenoproteins and brain metabolome as well as the possible connection with the gut-brain axis. To this end, an in vivo study with 40 BALB/c mice was carried out. The study included conventional ( n = 20) and mice model with microbiota depleted by antibiotics ( n = 20) under a regular or Se supplemented diet. Brain selenoproteome was determined by a transcriptomic/gene expression profile, while brain metabolome and gut microbiota profiles were accomplished by untargeted metabolomics and amplicon sequencing, respectively. The total content of Se in brain was also determined. The selenoproteins genes Dio and Gpx isoenzymes, SelenoH, SelenoI, SelenoT, SelenoV, and SelenoW and 31 metabolites were significantly altered in the brain after Se supplementation in conventional mice, while 11 selenoproteins and 26 metabolites were altered in microbiota depleted mice. The main altered brain metabolites were related to glyoxylate and dicarboxylate metabolism, amino acid metabolism, and gut microbiota that have been previously related with the gut-brain axis ( e.g., members of Lachnospiraceae and Ruminococcaceae families ). Moreover, specific associations were determined between brain selenoproteome and metabolome, which correlated with the same bacteria, suggesting an intertwined mechanism. Our results demonstrated the effect of Se on brain metabolome through specific selenoproteins gene expression and gut microbiota.This work was supported by the projects: PG2018-096608-B- C21 and PID2021-123073NB-C21 from the Spanish Ministry of Science and Innovation (MICIN) . Generación del Conocimiento . MCIN/ AEI /10.13039/50110 0 011033/ FEDER “Una manera de hacer Europa”, UHU-1256905 and UHU-202009 from the FEDER Andalusian Operative Program 2014-2020 (Ministry of Economy, Knowledge, Business and Universities, Regional Government of Andalusia, Spain). S.R.A. thanks the Spanish Ministry of Science and Innovation for a PhD scholarship ( BES-2016-076364 ). The authors are grateful to FEDER (European Community) for financial support, Grant UNHU13-1E-1611 . The authors would like to acknowledge the support from The Ramón Areces Foundation (ref. CIVP19A5918 ). Funding for open access charge: Universidad de Huelva / CBUA

Omic approaches in environmental risk assessment

Resumen de la comunicación presentada al XX Congreso de la Sociedad Española de Mutagénesis Ambiental – Córdoba 201

The treatment with the probiotic Shewanella putrefaciens Pdp11 of sepecimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease

Aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between stress and higher susceptibility to diseases has been concluded. Several studies have demonstrated increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not been elucidated. Shewanella putrefaciens Pdp11 is a strain isolated from healthy gilthead seabream (Sparus aurata L.) and it is considered as probiotics. The aim of this study was to evaluate the effect of the dietary administration of this probiotics on the stress tolerance of Solea senegalensis specimens farmed under high stocking density (PHD) compared to a group fed a commercial diet and farmed under the same conditions (CHD). In addition, during the experiment, a natural infectious outbreak due to Vibrio species affected fish farmed under crowding conditions. Changes in the microbiota and histology of intestine and in the transcription of immune response genes were evaluated at 19 and 30 days of the experiment. Mortality was observed after 9 days of the beginning of the experiment in CHD and PHD groups, it being higher in the CHD group. Fish farmed under crowding stress showed reduced expression of genes at 19 day probiotic feeding. On the contrary, a significant increase in immune related gene expression was detected in CHD fish at 30 day, whereas the gene expression in fish from PHD group was very similar to that showed in specimens fed and farmed with the conventional conditions. In addition, the dietary administration of S. putrefaciens Pdp11 produced an important modulation of the intestinal microbiota, which was significantly correlated with the high number of goblet cells detected in fish fed the probiotic diet.Postprin

Red LED Light Acts on the Mitochondrial Electron Chain of Mammalian Sperm via Light-Time Exposure-Dependent Mechanisms

This work analyzes the effects of red LED light on mammalian sperm mitochondrial function, using the pig as an animal model. Liquid-stored pig semen was stimulated with red-light for 1, 5 and 10 min in the presence or absence of oligomycin A, a specific inhibitor of mitochondrial ATP synthase, or carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), a specific disruptor of mitochondrial electron chain. Whereas exposure for 1 and 5 min significantly (p < 0.05) decreased total motility and intracellular ATP levels, irradiation for 10 min induced the opposite effect. Oligomycin A abolished the light-effects on intracellular ATP levels, O2 consumption and mitochondrial membrane potential, whereas compared to non-irradiated samples, FCCP significantly (p < 0.05) increased O2 consumption when sperm were irradiated for 1 min. Both oligomycin A and FCCP significantly (p < 0.05) decreased total motility. Red-light increased cytochrome c oxidase activity with a maximal effect after 5 min of irradiation, which was abolished by both oligomycin A and FCCP. In conclusion, red-light modulates sperm mitochondrial function via electron chain activity in an exposition, time-dependent manner

LPS modulates the expression of iron-related immune genes in two Antarctic notothenoids

The non-specific immunity can induce iron deprivation as a defense mechanism against potential bacterial pathogens, but little information is available as to its role in Antarctic fish. In this study the response of iron metabolism related genes was evaluated in liver and head kidney of the Antarctic notothenoids Notothenia coriiceps and Notothenia rossii 7 days after lipopolysaccharide (LPS) injection. Average plasma Fe2+ concentration was unaffected by treatment in any of the species. The gene expression response to LPS varied between tissues and species, being stronger in N. coriiceps and more prominent in the head kidney than liver. The reaction to LPS was marked by increased individual variability in most genes analyzed, even when the change in expression was not statistically significant, suggesting different individual sensitivity and coping responses in these wild fish. We found that iron related genes had an attenuated and homogenous response to LPS but there was no detectable relationship between plasma Fe2+ and gene expression. However, overall in both tissues and species LPS exposure set a multilevel response that concur to promote intracellular accumulation of iron, an indication that Antarctic Notothenoids use innate nutritional immunity as a resistance mechanism against pathogens.FCT-NSFC/0002/2016; CCMAR/Multi/04326/2013; PTDC/BIAANM/3484/2014info:eu-repo/semantics/publishedVersio

Anexo 2 - Ficha de Evaluación - Prácticum I y II - Grado en Trabajo Social

Innovación EducativaEste documento contiene el Anexo 2 de la memoria del Proyecto de Innovación Docente "Diseño y elaboración de rúbricas de evaluación de competencias del Trabajo Fin de Grado y del Prácticum en el Grado de Trabajo Social. Específicamente, este anexo contiene la rúbrica realizada para la evaluación del Prácticum I y Prácticum II del Grado en Trabajo Social de la Universidad de Valladolid.Departamento de Sociología y Trabajo SocialEste trabajo se ha llevado a cabo en el marco de la Convocatoria de Proyectos de Innovación Docente 2015-2016 - Universidad de Valladoli

Anexo 1 - Ficha de Evaluación - Trabajo Fin de Grado - Grado en Trabajo Social

Este documento contiene el Anexo 1 de la memoria del Proyecto de Innovación Docente "Diseño y elaboración de rúbricas de evaluación de competencias del Trabajo Fin de Grado y del Prácticum en el Grado de Trabajo Social". Específicamente, este anexo contiene la rúbrica realizada para la evaluación del Trabajo Fin de Grado del Grado en Trabajo Social.Departamento de Sociología y Trabajo SocialEste trabajo se ha llevado a cabo en el marco de la Convocatoria de Proyectos de Innovación Docente 2015-2016 - Universidad de Valladoli