From Class Assignment to Friendship: Enhancing the Intercultural Competence of Domestic and International Students through Experiential Learning

This study explored growth in the intercultural competence of domestic and international students who participated in an intercultural experiential learning initiative for academic credit. The initiative paired Canadian students in a second-year multiculturalism class at Wilfrid Laurier University with international students enrolled in the Laurier English and Academic Foundation (LEAF) program. Qualitative data derived from the oral and written reflections of three cohorts of students inform the study. The data were coded using pre-existing codes derived from learning objectives and reflection questions based on Deardorff’s (2006) Elements of Intercultural Competence Model. The findings suggest that while exposure to different cultural values and practices deepens domestic and international students’ knowledge and challenges their assumptions about each other, creating optimal conditions for meaningful intercultural contact between the students at a university may not adequately reflect everyday contact between them in complex real-life situations

Analysis of small RNA populationsgenerated in peanut leaves after exogenous application of dsRNA and dsDNA targeting aflatoxin synthesis genes

Previously, we have shown that RNA interference (RNAi) can prevent aflatoxin accumulation in transformed peanuts. To explore aflatoxin control by exogenous delivery of double-strand RNA (dsRNA) it is necessary to understand the generation of small RNA (sRNA) populations. We sequenced 12 duplicate sRNA libraries of in-vitro-grown peanut plants, 24 and 48 h after exogenous application of five gene fragments (RNAi-5x) related to aflatoxin biosynthesis in Aspergillus flavus. RNAi-5x was applied either as double-stranded RNA (dsRNA) or RNAi plasmid DNA (dsDNA). Small interfering RNAs (siRNAs) derived from RNAi-5x were significantly more abundant at 48 h than at 24 h, and the majority mapped to the fragment of aflatoxin efflux-pump gene. RNAi-5x-specific siRNAs were significantly, three to fivefold, more abundant in dsDNA than dsRNA treatments. Further examination of known micro RNAs related to disease-resistance, showed significant down-regulation of miR399 and up-regulation of miR482 in leaves treated with dsDNA compared to the control. These results show that sRNA sequencing is useful to compare exogenous RNAi delivery methods on peanut plants, and to analyze the efficacy of molecular constructs to generate siRNAs against specific gene targets. This work lays the foundation for non-transgenic delivery of RNAi in controlling aflatoxins in peanut

Assessing usability of a prototype soft exoskeleton by involving people with gait impairments

Background: Gait impairment is prevalent among many growing clinical populations e.g. people with stroke, incomplete spinal cord injury (iSCI), older adults etc. Such populations may benefit from assistive devices such as exoskeletons to improve their walking ability. XoSoft (www.xosoft.eu) is a soft exoskeleton that is being developed for people with mild to moderate gait impairments to support their mobility by providing physical actuation across joints of the lower extremities. During the design and development of a device like XoSoft, it is crucial that Primary Users (PUs, e.g. patients) are involved and provide insight into their experiences and expectations regarding device usability. However, it is still not standard practice to include PUs in rigorous testing of highly technical exoskeletons. The XoSoft consortium took an iterative design approach to the development of the XoSoft prototypes. Data from usability testing with PUs are informing next iterations of the XoSoft concepts. Purpose: The purpose of this study was to assess the PU experiences of the usability of a XoSoft prototype. This study should also highlight the importance of including PUs during the development of assistive devices. Methods: Eleven participants were recruited (mean age: 73 years, mean height: 166 cm, mean mass: 65 kg). There were three categories of PUs: frail (n=5), stroke (n=1), iSCI (n=5). Participants had no cognitive impairment (Mini Mental State Examination score > 24). The prototype consisted of a leggings-style garment with Velcro straps as anchor points for actuators across the relevant joints (hip, knee, ankle). Actuation and control was provided by a modular pneumatic/sensor controlled system, which was added to the garment in modular fashion based on the PU needs. After independent donning and doffing by the participant, the garment was donned and the Velcro straps placed and secured by a researcher to ensure proper placement. Participants then performed walking tasks with active actuation followed by completion of the System Usability Scale (SUS, Brooke 1996, maximum score = 100). Results: The scores for the SUS ranged from zero to 95 with a median rating of 52.5. The median rating corresponds to an “okay” score. According to the acceptability ranges by Bangor et al. (2008), 3 participants rated the prototype as acceptable, 3 as marginal, and 5 as not acceptable. Conclusions: The scores indicate the need for improvement in the design of future XoSoft prototypes. The large variability in SUS scores indicated that the same device may be rated considerably differently by different users. This highlights the importance of including a variety of potential users of assistive devices during development. Implications: Secondary Users (SUs) such as physical therapists are also involved in the development of XoSoft, providing insight into their own needs and the needs of their patients. The therapists also play a key role in motivating their patients to participate in research and development projects. To ensure that such assistive devices can be integrated into users’ lives and practices, it is essential to have both PUs and SUs actively involved in the technical development

Influence of providers and nurses on completion of non-targeted HIV screening in an urgent care setting

Introduction: Despite recommendations by the Centers for Disease Control (CDC) that all adults be offered non-targeted HIV screening in all care settings, screening in acute-care settings remains unacceptably low. We performed an observational study to evaluate an HIV screening pilot in an academic-community partnership health center urgent care clinic. Methods: We collected visit data via encounter forms and demographic and laboratory data from electronic medical records. A post-pilot survey of perceptions of HIV screening was administered to providers and nurses. Multivariable analysis was used to identify factors associated with completion of testing. Results: Visit provider and triage nurse were highly associated with both acceptance of screening and completion of testing, as were younger age, male gender, and race/ethnicity. 23.5% of patients completed tests, although 36.0% requested screening; time constraints as well as risk perceptions by both the provider and patient were cited as limiting completion of screening. Post-pilot surveys showed mixed support for ongoing HIV screening in this setting by providers and little support by nurses. Conclusions: Visit provider and triage nurse were strongly associated with acceptance of testing, which may reflect variable opinions of HIV screening in this setting by clinical staff. Among patients accepting screening, visit provider remained strongly associated with completion of testing. Despite longstanding recommendations for non-targeted HIV screening, further changes to improve the testing and results process, as well as provider education and buy-in, are needed to improve screening rates

Characterization of small RNA populations in non-transgenic andaflatoxin-reducing-transformed peanut

Aflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumula-tion of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNApopulations in a control line and in two transformed peanut lines that expressed an inverted repeattargeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B1 than the controls. The objective was to determine the putative involvement of the smallRNA populations in aflatoxin reduction. In total, 41 known microRNA (miRNA) families and many novel miRNAs were identified. Among those, 89 known and 10 novel miRNAs were differentially expressed in the transformed lines. We furthermore found two small interfering RNAs derived from the inverted repeat, and 39 sRNAs that mapped without mismatches to the genome of A. flavus and were present only in the transformed lines. This information will increase our understanding of the effectiveness of RNAi and enable the possible improvement of the RNAi technology for the control of aflatoxins

Basic functionality of a prototype wearable assistive soft exoskeleton for people with gait impairments : a case study

XoSoft is a soft modular wearable assistive exoskeleton for peo- ple with mild to moderate gait impairments. It is currently being developed by a European Consortium (www.xosoft.eu) and aims to provide tailored and active lower limb support during ambu- lation. During development, user-centered design principles were followed in parallel with the aim of providing functional support during gait. A prototype was developed and was tested for practi- cability, usability, comfort and assistive function (summarized as basic functionality) with a potential end user. The prototype con- sisted of a garment, electromagnetic clutch-controlled elastic bands supporting knee- and hip flexion and a backpack containing the sensor and actuator control of the system. The participant had ex- perienced a stroke and presented with unilateral impairment of the lower and upper extremities. In testing, he donned and doffed the prototype independently as far as possible, and performed walk- ing trials with the system in both active (powered on) and pas- sive (powered off) modes. Afterwards, the participant rated the perceived pressure and various elements of usability. Results high- lighted aspects of the system for improvement during future phases of XoSoft development, and also identified useful aspects of proto- type design to be maintained. The basic functionality of XoSoft could be assumed as satisfactory given that it was the first version of a working prototype. The study highlights the benefits of this participatory evaluation design approach in assistive soft robotics development

Exploring the dynamics of compliance with community penalties

In this paper, we examine how compliance with community penalties has been theorized hitherto and seek to develop a new dynamic model of compliance with community penalties. This new model is developed by exploring some of the interfaces between existing criminological and socio-legal work on compliance. The first part of the paper examines the possible definitions and dimensions of compliance with community supervision. Secondly, we examine existing work on explanations of compliance with community penalties, supplementing this by drawing on recent socio-legal scholarship on private individuals’ compliance with tax regimes. In the third part of the paper, we propose a dynamic model of compliance, based on the integration of these two related analyses. Finally, we consider some of the implications of our model for policy and practice concerning community penalties, suggesting the need to move beyond approaches which, we argue, suffer from compliance myopia; that is, a short-sighted and narrowly focused view of the issues

Combining energy efficiency measure approaches and occupancy patterns in building modelling in the UK residential context

The UK faces a significant retrofit challenge, especially with its housing stock of old, hard-to-treat solid walled dwellings. In this work, we investigate the delivery of heated thermal comfort with a lower energy demand through four types of energy efficiency interventions: passive system, conversion device, method of service control, and level of service demanded. These are compared for three distinct household occupancy patterns, corresponding to a working family, a working couple and a daytime-present couple. Energy efficiency measures are considered singly and in combination, to study whether multiple lower cost measures can achieve comparable savings to higher cost individual measures. Scenarios are simulated using engineering building modelling software TRNSYS with data taken from literature. Upgraded insulation of wall and roof resulted in highest savings in all occupancy scenarios, but comparable savings were calculated for reduced internal temperature and partial spatial heating in scenarios in which the house is not at maximum capacity. Zonal heating control is expected to achieve greatest savings for the working couple who had a flexible occupancy pattern. The results from this modelling work show the extent to which energy consumption depends on the appropriate matching between energy efficiency measures and occupant type

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy