Factors influencing the radiocarbon dating of human skeletal remains from the Dnieper River system: Archaeological and stable isotope evidence of diet from the Epipaleolithic to Eneolithic periods

Recent research has identified the existence of a freshwater reservoir effect influencing the radiocarbon dating of human skeletal remains from the Dnieper region of Ukraine (Lillie et al. 2009). The current study outlines the evidence for freshwater resource exploitation throughout the period ~10,200–3700 cal BC, and presents the available evidence for the existence of dietary offsets in the ¹⁴C dates obtained. We have obtained human skeletal material from 54 Epipaleolithic to Mesolithic period individuals and 267 Neolithic to Eneolithic individuals, from 13 cemeteries, since our research in Ukraine began in 1992. Here, we present the initial results of stable isotope analysis of Eneolithic individuals from the Igren VIII cemetery alongside the Epipaleolithic to Eneolithic samples that have previously been analyzed. When contrasted against the evidence from the prehistoric fauna and fish remains studied, and modern fish species from the Dnieper region, we continue to see variability in diets at the population level, both internally and across cemeteries. We also observed temporal variability in human diets across these chronological periods. The fish samples (both archaeological and modern) show a wide range of isotope ratios for both δ¹³C and δ¹⁵N, which could prove significant when interpreting the dietary sources being exploited. This information directly informs the ¹⁴C dating program as an inherent degree of complexity is introduced into the dating of individuals whose diets combine freshwater and terrestrial sources in differing quantities and at differing temporal and/or spatial scales (e.g. Bronk Ramsey et al. 2014

Mitochondrial DNA analysis of eneolithic trypillians from Ukraine reveals neolithic farming genetic roots

The agricultural revolution in Eastern Europe began in the Eneolithic with the Cucuteni-Trypillia culture complex. In Ukraine, the Trypillian culture (TC) existed for over two millennia (ca. 5,400–2,700 BCE) and left a wealth of artifacts. Yet, their burial rituals remain a mystery and to date almost nothing is known about the genetic composition of the TC population. One of the very few TC sites where human remains can be found is a cave called Verteba in western Ukraine. This report presents four partial and four complete mitochondrial genomes from nine TC individuals uncovered in the cave. The results of this analysis, combined with the data from previous reports, indicate that the Trypillian population at Verteba carried, for the most part, a typical Neolithic farmer package of mitochondrial DNA (mtDNA) lineages traced to Anatolian farmers and Neolithic farming groups of central Europe. At the same time, the find of two specimens belonging to haplogroup U8b1 at Verteba can be viewed as a connection of TC with the Upper Paleolithic European populations. At the level of mtDNA haplogroup frequencies, the TC population from Verteba demonstrates a close genetic relationship with population groups of the Funnel Beaker/ Trichterbecker cultural complex from central and northern Europe (ca. 3,950–2,500 BCE)

Prva analiza izotopov in novi radiokarbonski datumi poljedelcev kulture Tripolje iz jame Verteba, Bilche Zolote, Ukrajina

This paper presents an analysis of human and animal remains from Verteba cave, near Bilche Zolote, western Ukraine. This study was prompted by a paucity of direct dates on this material and the need to contextualise these remains in relation both to the transition from hunting and gathering to farming in Ukraine, and their specific place within the Cucuteni-Trypillia culture sequence. The new absolute dating places the remains studied here in Trypillia stages BII/CI at c. 3900–3500 cal BC, with one individual now redated to the Early Scythian period. As such, these finds are even more exceptional than previously assumed, being some of the earliest discovered for this culture. The isotope analyses indicate that these individuals are local to the region, with the dietary stable isotopes indicating a C3 terrestrial diet for the Trypillia-period humans analysed. The Scythian period individual has δ13C ratios indicative of either c. 50% marine, or alternatively C4 plant inputs into the diet, despite δ18O and 87Sr/86Sr ratios that are comparable to the other individuals studied.V članku predstavljamo analizo človeških in živalskih ostankov iz jame Verteba blizu kra­ja Bilche Zolote v zahodni Ukrajini. Študija je nastala zaradi pomanjkljivega števila datumov iz teh ostankov in iz potrebe po kontekstualiziranju najdb v odnosu tako do prehoda iz lovsko-nabiral­niške družbe v poljedelsko v Ukrajini kot tudi glede na poseben prostor, ki ga imajo te najdbe znotraj kulturne sekvence Cucuteni-Tripolje. Novi absolutni datumi postavljajo ostanke faze BII/CI kulture Tripolje v čas ok. 3900–3500 pr. n. št., medtem ko je eden od pokopov zdaj ponovno datiran v čas zgod­­njega skitskega obdobja, kar pomeni, da gre za ene najstarejših najdb te kulture. Analiza izoto­pov kaže, da so bili pokojni lokalni prebi­val­ci regije, analiza stabilnih izotopov pa kaže na C3 ko­pen­sko prehrano ljudi v času kulture Tri­po­lje. Pokop iz skitskega obdobja kaže glede na δ13C vredno­sti na ok. 50 % morske prehrane, oziroma na C4 rastline, vključene v prehrano, kljub temu da so deleži izotopov δ18O in 87Sr/86Sr primerljivi s preo­sta­limi vzorci ljudi na najdišču

Genetic continuity, isolation, and gene flow in Stone Age Central and Eastern Europe

Abstract The genomic landscape of Stone Age Europe was shaped by multiple migratory waves and population replacements, but different regions do not all show the same patterns. To refine our understanding of the population dynamics before and after the dawn of the Neolithic, we generated and analyzed genomic sequence data from human remains of 56 individuals from the Mesolithic, Neolithic and Eneolithic across Central and Eastern Europe. We found that Mesolithic European populations formed a geographically widespread isolation-by-distance zone ranging from Central Europe to Siberia, which was already established 10 000 years ago. We also found contrasting patterns of population continuity during the Neolithic transition: people around the lower Dnipro Valley region, Ukraine, showed continuity over 4 000 years, from the Mesolithic to the end of Neolithic, in contrast to almost all other parts of Europe where population turnover drove this cultural change, including vast areas of Central Europe and around the Danube River

Population genomics of the Viking world.

The maritime expansion of Scandinavian populations during the Viking Age (about AD 750-1050) was a far-flung transformation in world history1,2. Here we sequenced the genomes of 442 humans from archaeological sites across Europe and Greenland (to a median depth of about 1×) to understand the global influence of this expansion. We find the Viking period involved gene flow into Scandinavia from the south and east. We observe genetic structure within Scandinavia, with diversity hotspots in the south and restricted gene flow within Scandinavia. We find evidence for a major influx of Danish ancestry into England; a Swedish influx into the Baltic; and Norwegian influx into Ireland, Iceland and Greenland. Additionally, we see substantial ancestry from elsewhere in Europe entering Scandinavia during the Viking Age. Our ancient DNA analysis also revealed that a Viking expedition included close family members. By comparing with modern populations, we find that pigmentation-associated loci have undergone strong population differentiation during the past millennium, and trace positively selected loci-including the lactase-persistence allele of LCT and alleles of ANKA that are associated with the immune response-in detail. We conclude that the Viking diaspora was characterized by substantial transregional engagement: distinct populations influenced the genomic makeup of different regions of Europe, and Scandinavia experienced increased contact with the rest of the continent

SOUTH-EASTERN INFLUENCES ON THE FORMATION OF THE MESOLITHIC TO EARLY ENEOLITHIC POPULATIONS OF THE NORTH PONTIC REGION: THE EVIDENCE FROM ANTHROPOLOGY

This volume contains the majority of the papers presented during a conference that took place on 16th-21st May, 1997 in Łódź, Poland. The conference was organized by the Institute of Archaeology, University of Łódź and Département d'anthropologie, Université de Montreal (Canada). The conference was funded by the University of Łódź and by IREX (International Research & Exchanges Board), which also supported this publication. The publication was partly founded by the University of Łódź and by the Foundation of Adam Mickiewicz University, too. The major questions of the conference were, 1) what is the current evidence for eastern or southern influences in the development of eastern European Mesolithic and Neolithic populations, and 2) to what extent are current political trends, especially the reassertion or, in some cases, the creation of ethnic and national identities, influencing our interpretations of the prehistoric data. The idea for such a conference came into being through the co-organizers' long-term studies of the development of those prehistoric human populations which inhabited the vast region stretching north and east from the Oder river and Carpathian Mountains to the foothills of the Urals. In a tradition established in modern times by Gordon Childe, virtually all of the transformations of Eastern Europe's Neolithic Age human landscape have been assumed to be responses to prior developments in the Balkan peninsula and Danube basin. We think that a body of new evidence requires a renewed analysis of the distributions of cultural products, peoples, and ideas across Eastern Europe during the Mesolithic through the Early Metal Age within a much wider geographic context than previously has been the case. This includes giving adequate attention to the far-ranging interactions of communities between the Pontic and Baltic area with those located in both the Caucasus and the Aralo-Caspian regions. We hope that this volume will contribute to such a redirection of future analyses

Radiocarbon dating inconsistencies of a late Mesolithic burial site at Gard, Ukraine

A multi-layer site Gard in southern Ukraine presents a unique record of prehistoric occupation of southeast Europe from the Mesolithic to modern times. An early Bronze Age (EBA) cemetery and a tumulus group, as well as a late Mesolithic burial of extended (Mariupol-type, M-t) interments was uncovered at the site in 2013. Cultural layer between the M-t interments and EBA cemetery has been previously dated by C14 analysis to the Neolithic (Bug-Dnister and Trypillia cultural complexes). Three interments from the M-t cemetery and two from the EBA cemetery have recently been subjected to C14 analysis. The EBA dates fell within the expected range. The M-t burials, on the other hand, deviated from the predicted based on archaeology findings by two millennia. Gard is situated on a riverbank, thus the artifacts found at the site have been most likely subjected to repeated floodings. Furthermore, it is likely that the diet of the inhabitants likely included foodstuffs derived from the river. Thus, the reservoir effect on C14 dates has been expected. However, the reservoir effect would age the specimens, not make them younger. Potential factors that influence the C14 dating at the site remain unclear

Continuation of fishing subsistence in the Ukrainian Neolithic : diet isotope studies at Yasinovatka, Dnieper Rapids

Yasinovatka is one of around 30 number of prehistoric cemetery sites of hunter-fisher-foragers located along the Dnieper River in southern Ukraine. Dating to c. 5540 - 4930 cal BC, the skeletal remains at Yasinovatka suggest that around sixty-eight individuals were interred at the cemetery, during three broad phases of interment: A-type burials (c. 5540-4930 cal BC), Ƃ1 pit burials (c. 5550-4750 cal BC), and Ƃ2 pit burials (c. 4980-4460 cal BC). The burials are characterized, in part, by the inclusion of a number of Mariupol-type plates of boar tusk, in addition to deer tooth pendants, Unio shells, knife-like flint blades, Cyprinidae teeth, sherds of Neolithic pottery, and significant deposits of ochre in the later burial pits. Here we analyse δ13C and δ15N values for 50 human bone collagen samples from the site.  The majority of the isotope results show a hunter-fisher-forager population reliant predominantly on freshwater aquatic proteins, which is in keeping with previous dietary isotope studies in the area. Two individuals however have δ15N values that are clearly depleted when compared to the main population; these reflect dietary protein intakes based on plant and animal terrestrial resources rather than the predominant focus on aquatic resources. Notably, the δ13C values of these anomalous individuals are not enriched compared to the fauna samples analysed from the region; this supports the possibility that they were incomers to the area, potentially from a nearby agrarian population

Stable isotope analysis of prehistoric populations from the cemeteries of the Middle and Lower Dnieper Basin, Ukraine

This paper presents the results of new stable isotopes (carbon and nitrogen) analysis of human, faunal and fish remains from thirteen cemeteries from the Middle and Lower Dnieper Basin, Ukraine. The results are integrated with earlier analyses, undertaken solely on human samples, in order to provide a comprehensive overview of subsistence across the Upper Palaeolithic through to Eneolithic periods in this region. This is the first time that a combined sample of human, faunal and fish remains has been studied in order to interpret subsistence strategies across these periods in Ukraine. The total dataset comprises 113 samples of human, faunal and fish remains, 59 of which include new analyses of faunal and fish remains that have not previously been made available for study (Table 1). The analysis of the faunal and fish remains allows for a consideration of trophic levels which indicates that the consumption of freshwater fish occurs from the Epipalaeolithic period onwards. Whilst the majority of the cemeteries and the individuals therein cluster in relatively tight groups, there is a significant offset between the human and faunal samples due to the consumption of freshwater resources. The fish samples analysed in the study appear to exhibit a relatively random distribution when compared to the other samples analysed. In general, freshwater resources remain significant through until the end of the Neolithic period and into the Eneolithic period at the sites investigated