Haalbaarheid van LED-tussenbelichting bij roos: praktijkonderzoek op Marjoland

Doelstelling van dit onderzoek is een mechanische aanpk van tussenbelichting bij roos, waarbij onderscheid werd gemaakt tussen groeilicht-, stuurlicht- en temperastuureffecten binnen een gewas

Enterococcus cecorum septicemia in a malnourished adult patient

Enterococcus cecorum, a species typically isolated from chicken, pigs, calves, horses, ducks, cats, dogs, and canaries, was isolated from the blood of a patient with severe septicemia. The isolate was identified by conventional biochemical tests. Identity asEnterococcus cecorum was confirmed by SDS-PAGE analysis of whole cell protein. This is the first report of the isolation ofEnterococcus cecorum in a clinical settin

PERFORMANCE OF TIMBER BOARD MODELS FOR PREDICTION OF LOCAL BENDING STIFFNESS AND STRENGTH – WITH APPLICATION ON DOUGLAS FIR SAWN TIMBER

Efficient utilization of structural timber requires accurate methods for machine strength grading. One of the most accurate methods presented this far is based on data of local fiber orientation on board surfaces, obtained from laser scanning. In this paper, two potential improvements of this method are examined. The first one consists of replacing a model based on simple integration over cross sections of boards for calculation of local bending stiffness by a 3D solid finite element (FE) model from which local bending stiffness is derived. The second improvement concerns replacement of a simple model for the fiber orientation in the interior of board by a more advanced one taking location of pith and growth direction of knots into account. Application of the alternative models on a sample of more than 200 Douglas fir boards, size 40 mm X 100 mm X 3000 mm, cut from large logs, show that each of the evaluated model improvements contributes to improved grading accuracy. When local bending stiffness is calculated utilizing the herein suggested FE model in combination with the improved model of fiber orientation in the interior of boards, a coefficient of determination to bending strength as high as 0.76 is obtained. For comparison, a coefficient of determination of 0.71 is obtain using the simpler original models

Análise da diversidade nucleotídica intra e interespecífica de Coffea spp.

Os bancos de dados de ESTs do gênero #Coffea# têm auxiliado os estudos de identificação de marcadores moleculares. Entre estes trabalhos, foi desenvolvido um #pipeline# para busca de polimorfismos que resultou na identificação de 23.062 SNPs e INDELS presentes em dois genótipos de #C. arabica# e quatro de #C canephora#. Devido a baixa representatividade genotípica dos dados de ESTs, esse trabalho buscou validar os polimorfismos detectados pelo #pipeline# e avaliar a frequência dos mesmos em um painel maior de genótipos, representativo da diversidade de #C. arabica# (12 genótipos) e #C. canephora# (oito genótipos). Também foram avaliados os polimorfismos entre estas duas espécies com #C. eugenioides#, #C. racemosa# e #Psilanthus bengalensis#. Para a análise foram selecionados oito genes envolvidos na biossíntese de diterpenos e açúcares, importantes compostos relacionados à qualidade da bebida, bem como um gene mitocondrial e um cloroplastídico, que permitem também a inferência sobre a história evolutiva dos genes analisados. Fragmentos dos respectivos genes foram amplificados por PCR e os produtos foram sequenciados. O alinhamento das sequências e a detecção manual dos polimorfismos foram realizados pelo programa #Codon Code Aligner#. Aproximadamente 7,6 kb foram explorados para identificação de 465 polimorfismos incluindo 416 SNPs, 18 INDELs e 31 SSR. Uma frequência de 6,1 SNP foi observada a cada 100 pb. Quando todos os polimorfismos foram considerados, verificou-se que 110 correspondem a diferenças entre #Psilanthus# e #Coffea# e 360 correspondem a diferenças entre as espécies de Coffea; destes 266 são intra ou inter específicos para #C. canephora# e #C. eugenioides#, espécies ancestrais de C. arabica. Em C. arabica foram encontrados 134 SNPs, sendo que aproximadamente 87% destes correspondem a polimorfismos entre os parentais da espécie e apenas 13% correspondem a diferenças intraespecíficas, sendo que maioria dos polimorfismos intraespecíficos verificados não é fixada na população. A partir dos resultados inferiu-se que a análise realizada pelo #pipeline# foi relevante para identificar alguns polimorfismos que diferenciam os genomas dos ancestrais de #C. arabica#, mas apresenta limitações na detecção de SNPs intraespecíficos importantes; por outro lado, o painel diverso de genótipos, permitiu a detecção de vários polimorfismos ainda não identificados, importantes para o mapeamento genético, análises de diversidade e de evolução molecular ao nível intraespecífico. Os dados obtidos poderão ser utilizados tanto nos programas de mapeamento genético de #C. arabica# e #C. canephora#, como para aplicações práticas de genotipagem ou no melhoramento através de seleção assistida por marcadores (SAM), além de serem úteis para estudos evolutivos e possibilitarem a escolha de genes candidatos para estudos de associação e de expressão diferencial de haplótipos relacionada à plasticidade fenotípica. Apoio Financeiro: Consórcio Pesquisa Café, CNPq. (Texte intégral

Characterization and expression of two cDNA encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase isoforms in coffee (Coffea arabica L.).

In higher plants there are two independent pathways for isoprenoid biosynthesis, located in the cytosol (mevalonic acid or MVA pathway) or in the plastids (methylerythritol phosphate ? MEP pathway). The 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) is the first committed step in the MVA pathway. Using the information available from the Brazilian Coffee Genome Project, we found 13 ESTs that originated two isoforms, CaHMGR1 and CaHMGR2, for the enzyme HMGR of Coffea arabica. A complementary DNA encoding the isoform CaHMGR1 was cloned, and its complete nucleotide sequence determined. The full-length cDNA of CaHMGR1 was 2242 bp containing a 1812-bp ORF encoding 604 amino acids. Bioinformatic analyses revealed that the deduced CaHMGR1 had extensive homology with other plant HMGRs and contained two transmembrane domains and two putative HMGR binding sites and two NADP(H)-binding sites. Under normal growth conditions, transcripts of isoform CaHMRG1 were detected in fruit tissues (pulp, perisperm and endosperm) only at the initial stages of development, flower buds and leaves. CaHMRG2 was expressed in all tissues and during all fruit development stages examined. These results suggest a constitutive expression of isoform CaHMGR2, while the isoform CaHMGR1 shows temporal and tissue-specific transcriptional activation

Systematically Developing a Web-Based Tailored Intervention Promoting HPV-Vaccination Acceptability Among Mothers of Invited Girls Using Intervention Mapping.

Background: Currently, the eHealth field calls for detailed descriptions of theory-based interventions in order to support improved design of such interventions. This article aims to provide a systematic description of the design rationale behind an interactive web-based tailored intervention promoting HPV-vaccination acceptability. Methods: The 6-step Intervention Mapping (IM) protocol was used to describe the design rationale. After the needs assessment in Step 1, intervention objectives were formulated in Step 2. In Step 3, we translated theoretical methods into practical applications, which were integrated into a coherent intervention in Step 4. In Step 5, we anticipated future implementation and adoption, and finally, an evaluation plan was generated in Step 6. Results: Walking through the various steps of IM resulted in a detailed description of the intervention. The needs assessment indicated HPV-vaccination uptake remaining lower than expected. Mothers play the most important role in decision-making about their daughter's immunization. However, they generally feel ambivalent after they made their decisions, and their decisions are based on rather unstable grounds. Therefore, intervention objectives were to improve HPV-vaccination uptake and informed decision-making, and to decrease decisional conflict among mothers of invited girls. Computer-tailoring was chosen as the main method; virtual assistants were chosen as a practical application to deliver interactive tailored feedback. To maximize compatibility with the needs of the target group, a user-centered design strategy by means of focus groups and online experiments was applied. In these, prototypes were tested and sequentially refined. Finally, efficacy, effectiveness, and acceptability of the intervention were tested in a randomized controlled trial. Results showed a significant positive effect of the intervention on informed decision-making, decisional conflict, and nearly all determinants of HPV-vaccination uptake (P < 0.001). Mothers evaluated the intervention as highly positive. Discussion: Using IM led to an innovative effective intervention for promoting HPV-vaccination acceptability. The intervention maps will aid in interpreting the results of our evaluation studies. Moreover, it will ease the comparison of design rationales across interventions, and may provide leads for the development of other eHealth interventions. This paper adds to the plea for systematic reporting of design rationales constituting the process of developing interventions

A Quantitative Trait Locus Analysis of Natural Genetic Variation for \u3ci\u3eDrosophila melanogaster\u3c/i\u3e Oxidative Stress Survival

Little is known about natural genetic variation for survival under oxidative stress conditions or whether genetic variation for oxidative stress survival is associated with that for life-history traits. We have investigated survival in a high-oxygen environment at 2 adult densities using a set of re-combinant inbred lines (RILs) isolated from a natural population of Drosophila melanogaster. Female and male oxidative stress survival was highly correlated. Quantitative trait loci (QTLs) for oxidative stress survival were identified on both autosomes. These QTLs were sometimes sex or density spe-cific but were most often not. QTLs were identified that colocalize to the same region of the genome as longevity in other studies using the same set of RILs. We also determined early-age egg produc-tion and found QTLs for this trait, but there was no support for an association between oxidative stress survival and egg production

A Quantitative Trait Locus Analysis of Natural Genetic Variation for \u3ci\u3eDrosophila melanogaster\u3c/i\u3e Oxidative Stress Survival

Little is known about natural genetic variation for survival under oxidative stress conditions or whether genetic variation for oxidative stress survival is associated with that for life-history traits. We have investigated survival in a high-oxygen environment at 2 adult densities using a set of re-combinant inbred lines (RILs) isolated from a natural population of Drosophila melanogaster. Female and male oxidative stress survival was highly correlated. Quantitative trait loci (QTLs) for oxidative stress survival were identified on both autosomes. These QTLs were sometimes sex or density spe-cific but were most often not. QTLs were identified that colocalize to the same region of the genome as longevity in other studies using the same set of RILs. We also determined early-age egg produc-tion and found QTLs for this trait, but there was no support for an association between oxidative stress survival and egg production