Evaluation of cystatin C for the detection of chronic kidney disease in cats

BackgroundSerum cystatin C (sCysC) and urinary cystatin C (uCysC) are potential biomarkers for early detection of chronic kidney disease (CKD) in cats. An in-depth clinical validation is required. ObjectivesTo evaluate CysC as a marker for CKD in cats and to compare assay performance of the turbidimetric assay (PETIA) with the previously validated nephelometric assay (PENIA). AnimalsNinety cats were included: 49 CKD and 41 healthy cats. MethodsSerum CysC and uCysC concentrations were prospectively evaluated in cats with CKD and healthy cats. Based on plasma exo-iohexol clearance test (PexICT), sCysC was evaluated to distinguish normal, borderline, and low GFR. Sensitivity and specificity to detect PexICT<1.7mL/min/kg were calculated. Serum CysC results of PENIA and PETIA were correlated with GFR. Statistical analysis was performed using general linear modeling. ResultsCats with CKD had significantly higher meanSD sCysC (1.4 +/- 0.5mg/L) (P<.001) and uCysC/urinary creatinine (uCr) (291 +/- 411mg/mol) (P<.001) compared to healthy cats (sCysC 1.0 +/- 0.3 and uCysC/uCr 0.32 +/- 0.97). UCysC was detected in 35/49 CKD cats. R-2 values between GFR and sCysC or sCr were 0.39 and 0.71, respectively (sCysC or sCr=+GFR+epsilon). Sensitivity and specificity were 22 and 100% for sCysC and 83 and 93% for sCr. Serum CysC could not distinguish healthy from CKD cats, nor normal from borderline or low GFR, in contrast with sCr. ConclusionSerum CysC is not a reliable marker of reduced GFR in cats and uCysC could not be detected in all CKD cats

Hypergraphic LP Relaxations for Steiner Trees

We investigate hypergraphic LP relaxations for the Steiner tree problem, primarily the partition LP relaxation introduced by Koenemann et al. [Math. Programming, 2009]. Specifically, we are interested in proving upper bounds on the integrality gap of this LP, and studying its relation to other linear relaxations. Our results are the following. Structural results: We extend the technique of uncrossing, usually applied to families of sets, to families of partitions. As a consequence we show that any basic feasible solution to the partition LP formulation has sparse support. Although the number of variables could be exponential, the number of positive variables is at most the number of terminals. Relations with other relaxations: We show the equivalence of the partition LP relaxation with other known hypergraphic relaxations. We also show that these hypergraphic relaxations are equivalent to the well studied bidirected cut relaxation, if the instance is quasibipartite. Integrality gap upper bounds: We show an upper bound of sqrt(3) ~ 1.729 on the integrality gap of these hypergraph relaxations in general graphs. In the special case of uniformly quasibipartite instances, we show an improved upper bound of 73/60 ~ 1.216. By our equivalence theorem, the latter result implies an improved upper bound for the bidirected cut relaxation as well.Comment: Revised full version; a shorter version will appear at IPCO 2010

Temporal Variability of Diapycnal Mixing in Shag Rocks Passage

Diapycnal mixing rates in the oceans have been shown to have a great deal of spatial variability, but the temporal variability has been little studied. Here we present results from a method developed to calculate diapycnal diffusivity from moored Acoustic Doppler Current Profiler (ADCP) velocity shear profiles. An 18-month time series of diffusivity is presented from data taken by a LongRanger ADCP moored at 2400 m depth, 600 m above the sea floor, in Shag Rocks Passage, a deep passage in the North Scotia Ridge (Southern Ocean). The Polar Front is constrained to pass through this passage, and the strong currents and complex topography are expected to result in enhanced mixing. The spatial distribution of diffusivity in Shag Rocks Passage deduced from lowered ADCP shear is consistent with published values for similar regions, with diffusivity possibly as large as 90 × 10-4 m2 s-1 near the sea floor, decreasing to the expected background level of ~ 0.1 × 10-4 m2 s-1 in areas away from topography. The moored ADCP profiles spanned a depth range of 2400 to 1800 m; thus the moored time series was obtained from a region of moderately enhanced diffusivity. The diffusivity time series has a median of 3.3 × 10-4 m2 s-1 and a range of 0.5 × 10-4 m2 s-1 to 57 × 10-4 m2 s-1. There is no significant signal at annual or semiannual periods, but there is evidence of signals at periods of approximately fourteen days (likely due to the spring-neaps tidal cycle), and at periods of 3.8 and 2.6 days most likely due to topographically-trapped waves propagating around the local seamount. Using the observed stratification and an axisymmetric seamount, of similar dimensions to the one west of the mooring, in a model of baroclinic topographically-trapped waves, produces periods of 3.8 and 2.6 days, in agreement with the signals observed. The diffusivity is anti-correlated with the rotary coefficient (indicating that stronger mixing occurs during times of upward energy propagation), which suggests that mixing occurs due to the breaking of internal waves generated at topography

Design of a Low-Energy FARAD Thruster

The design of an electrodeless thruster that relies on a pulsed, rf-assisted discharge and electromagnetic acceleration using an inductive coil is presented. The thruster design is optimized using known performance,scaling parameters, and experimentally-determined design rules, with design targets for discharge energy, plasma exhaust velocity; and thrust efficiency of 100 J/pulse, 25 km/s, and 50%, respectively. Propellant is injected using a high-speed gas valve and preionized by a pulsed-RF signal supplied by a vector inversion generator, allowing for current sheet formation at lower discharge voltages and energies relative to pulsed inductive accelerators that do not employ preionization. The acceleration coil is designed to possess an inductance of at least 700 nH while the target stray (non-coil) inductance in the circuit is 70 nH. A Bernardes and Merryman pulsed power train or a pulse compression power train provide current to the acceleration coil and solid-state components are used to switch both powertrains

Cardiovascular-renal axis disorders in the domestic dog and cat: a veterinary consensus statement

OBJECTIVES There is a growing understanding of the complexity of interplay between renal and cardiovascular systems in both health and disease. The medical profession has adopted the term "cardiorenal syndrome" (CRS) to describe the pathophysiological relationship between the kidney and heart in disease. CRS has yet to be formally defined and described by the veterinary profession and its existence and importance in dogs and cats warrant investigation. The CRS Consensus Group, comprising nine veterinary cardiologists and seven nephrologists from Europe and North America, sought to achieve consensus around the definition, pathophysiology, diagnosis and management of dogs and cats with "cardiovascular-renal disorders" (CvRD). To this end, the Delphi formal methodology for defining/building consensus and defining guidelines was utilised. METHODS Following a literature review, 13 candidate statements regarding CvRD in dogs and cats were tested for consensus, using a modified Delphi method. As a new area of interest, well-designed studies, specific to CRS/CvRD, are lacking, particularly in dogs and cats. Hence, while scientific justification of all the recommendations was sought and used when available, recommendations were largely reliant on theory, expert opinion, small clinical studies and extrapolation from data derived from other species. RESULTS Of the 13 statements, 11 achieved consensus and 2 did not. The modified Delphi approach worked well to achieve consensus in an objective manner and to develop initial guidelines for CvRD. DISCUSSION The resultant manuscript describes consensus statements for the definition, classification, diagnosis and management strategies for veterinary patients with CvRD, with an emphasis on the pathological interplay between the two organ systems. By formulating consensus statements regarding CvRD in veterinary medicine, the authors hope to stimulate interest in and advancement of the understanding and management of CvRD in dogs and cats. The use of a formalised method for consensus and guideline development should be considered for other topics in veterinary medicine

Testing of Liquid Metal Components for Nuclear Surface Power Systems

The capability to perform testing at both the module/component level and in near prototypic reactor configurations using a non-nuclear test methodology allowed for evaluation of two components critical to the development of a potential nuclear fission power system for the lunar surface. A pair of 1 kW Stirling power convertors, similar to the type that would be used in a reactor system to convert heat to electricity, were integrated into a reactor simulator system to determine their performance using pumped NaK as the hot side working fluid. The performance in the pumped-NaK system met or exceed the baseline performance measurements where the converters were electrically heated. At the maximum hot-side temperature of 550 C the maximum output power was 2375 watts. A specially-designed test apparatus was fabricated and used to quantify the performance of an annular linear induction pump that is similar to the type that could be used to circulate liquid metal through the core of a space reactor system. The errors on the measurements were generally much smaller than the magnitude of the measurements, permitting accurate performance evaluation over a wide range of operating conditions. The pump produced flow rates spanning roughly 0.16 to 5.7 l/s (2.5 to 90 GPM), and delta p levels from less than 1 kPa to 90 kPa (greater than 0.145 psi to roughly 13 psi). At the nominal FSP system operating temperature of 525 C the maximum efficiency was just over 4%

Global biogeography of chemosynthetic symbionts reveals both localized and globally distributed

In the ocean, most hosts acquire their symbionts from the environment. Due to the immense spatial scales involved, our understanding of the biogeography of hosts and symbionts in marine systems is patchy, although this knowledge is essential for understanding fundamental aspects of symbiosis such as host-symbiont specificity and evolution. Lucinidae is the most species-rich and widely distributed family of marine bivalves hosting autotrophic bacterial endosymbionts. Previous molecular surveys identified location-specific symbiont types that "promiscuously" form associations with multiple divergent cooccurring host species. This flexibility of host-microbe pairings is thought to underpin their global success, as it allows hosts to form associations with locally adapted symbionts. We used metagenomics to investigate the biodiversity, functional variability, and genetic exchange among the endosymbionts of 12 lucinid host species from across the globe. We report a cosmopolitan symbiont species, Candidatus Thiodiazotropha taylori, associated with multiple lucinid host species. Ca. T. taylori has achieved more success at dispersal and establishing symbioses with lucinids than any other symbiont described thus far. This discovery challenges our understanding of symbiont dispersal and location-specific colonization and suggests both symbiont and host flexibility underpin the ecological and evolutionary success of the lucinid symbiosis

Observed eddy-internal wave interactions in the Southern Ocean

The physical mechanisms that remove energy from the Southern Ocean’s vigorous mesoscale eddy field are not well understood. One proposed mechanism is direct energy transfer to the internal wave field in the ocean interior, via eddy-induced straining and shearing of preexisting internal waves. The magnitude, vertical structure, and temporal variability of the rate of energy transfer between eddies and internal waves is quantified from a 14-month deployment of a mooring cluster in the Scotia Sea. Velocity and buoyancy observations are decomposed into wave and eddy components, and the energy transfer is estimated using the Reynolds-averaged energy equation. We find that eddies gain energy from the internal wave field at a rate of −2.2 ± 0.6 mW m−2, integrated from the bottom to 566 m below the surface. This result can be decomposed into a positive (eddy to wave) component, equal to 0.2 ± 0.1 mW m−2, driven by horizontal straining of internal waves, and a negative (wave to eddy) component, equal to −2.5 ± 0.6 mW m−2, driven by vertical shearing of the wave spectrum. Temporal variability of the transfer rate is much greater than the mean value. Close to topography, large energy transfers are associated with low-frequency buoyancy fluxes, the underpinning physics of which do not conform to linear wave dynamics and are thereby in need of further research. Our work suggests that eddy–internal wave interactions may play a significant role in the energy balance of the Southern Ocean mesoscale eddy and internal wave fields