Machine learning: Lighting up protein design

Using a neural network to predict how green fluorescent proteins respond to genetic mutations illuminates properties that could help design new proteins

Coarse-graining schemes and a posteriori error estimates for stochastic lattice systems

The primary objective of this work is to develop coarse-graining schemes for stochastic many-body microscopic models and quantify their effectiveness in terms of a priori and a posteriori error analysis. In this paper we focus on stochastic lattice systems of interacting particles at equilibrium. %such as Ising-type models. The proposed algorithms are derived from an initial coarse-grained approximation that is directly computable by Monte Carlo simulations, and the corresponding numerical error is calculated using the specific relative entropy between the exact and approximate coarse-grained equilibrium measures. Subsequently we carry out a cluster expansion around this first-and often inadequate-approximation and obtain more accurate coarse-graining schemes. The cluster expansions yield also sharp a posteriori error estimates for the coarse-grained approximations that can be used for the construction of adaptive coarse-graining methods. We present a number of numerical examples that demonstrate that the coarse-graining schemes developed here allow for accurate predictions of critical behavior and hysteresis in systems with intermediate and long-range interactions. We also present examples where they substantially improve predictions of earlier coarse-graining schemes for short-range interactions.Comment: 37 pages, 8 figure

Nonequilibrium Polaritonics - Nonlinear Effects and Optical Switching

We report a theoretical non-equilibrium description of polaritonics and we propose ultrafast all- optical switching due to highly nonlinear polaritonics. The electronic band structure within gold (Au) nano grains is modified by external laser light. The Au grains are coupled to a single mode photonic waveguide and we derive a strong transmission reduction of switching originating from the establishd quantum interference with a finite lifetime.Comment: 8 pages 8 figure

Generation of stable, non-aggregating Saccharomyces cerevisiae wild isolates

Cellular aggregates observed during growth of Saccharomyces cerevisiae strains derived from various natural environments makes most laboratory techniques optimized for non-aggregating laboratory strains inappropriate. We describe a method to reduce the size and percentage of the aggregates. This is achieved by replacing the native allele of the AMN1 gene with an allele found in the W303 laboratory strain. The reduction in aggregates is consistent across various environments and generations, with no change in maximum population density or strain viability, and only minor changes in maximum growth rate and colony morphology

The Performance of Real Estate Activities

Diplomová práce se zabývá problémem silných a slabých stránek realitního trhnu na území České republiky. Vymezuje základní pojmy a definice spojené s realitní činností. Analyzuje chyby realitních společností. Poskytuje prady pro předcházení chyb. Zveřejňuje statistické informace o realitním trhu. Získané data jsou statisticky vyhodnoceny a zpracovány do grafické podoby. Tyto získané údaje jsou určené pro praktické využití.This the sisdeals with the problem of the strengths and weaknesses of real estate in area the Czech Republic. It defines the basic terms and definitions as sociated with real estate activities. It analyzes the mistakes of real estate companies. Provides tips for preventing errors. Publishes statistical information about the property market. The data are statistically evaluated and incorporated into the graphics. These obtained datas are intended for practical use for the valuation of yield method.

A Shack-Hartmann sensor for single-shot multi-contrast imaging with hard X-rays

An array of compound refractive X-ray lenses (CRL) with 20x20 lenslets, a focal distance of 20 cm and a visibility of 0.93 is presented. It can be used as a Shack-Hartmann sensor for hard X-rays (SHARX) for wavefront sensing and permits for true single-shot multi-contrast imaging the dynamics of materials with a spatial resolution in the micrometer range, sensitivity on nanosized structures and temporal resolution on the microsecond scale. The object's absorption and its induced wavefront shift can be assessed simultaneously together with information from diffraction channels. This enables the imaging of hierarchical materials. In contrast to the established Hartmann sensors the SHARX has an increased flux efficiency through focusing of the beam rather than blocking parts of it. We investigated the spatiotemporal behavior of a cavitation bubble induced by laser pulses. Furthermore, we validated the SHARX by measuring refraction angles of a single diamond CRL, where we obtained an angular resolution better than 4 microrad