Passifloraceae na Serra Negra, Minas Gerais, Brasil

In this study are presented the species of Passifloraceae from Serra Negra, a part of Serra da Mantiqueira located in South Zona da Mata of Minas Gerais, in the coordinates 21º58'11"S and 43º53'21"W. The vegetation is represented by a mosaic of "campos rupestres" and semideciduous forests and rainforests. The family is represented by 12 species that belong to the genus Passiflora: P. alata Curtis, P. amethystina J.C.Mikan, P. campanulata Mast., P. capsularis L., P. edulis Sims, P. haematostigma Mart. ex Mast., P. marginata Mast., P. mediterranea Vell., P. porophylla Vell., P. sidifolia M.Roem., P. speciosa Gardner and a species not identified, probably a new taxon. To recognize the species, identification key to species, descriptions, illustrations, geographical distribution and taxonomical comments are provided.Neste trabalho são apresentadas as espécies de Passifloraceae da Serra Negra, parte da Serra da Mantiqueira localizada no sul da Zona da Mata de Minas Gerais, nas coordenadas 21º58'11"S e 43º53'21"W. A vegetação é representada por um mosaico de campos rupestres e florestas semidecíduas e ombrófilas. A família está representada por 12 espécies pertencentes ao gênero Passiflora: P. alata Curtis, P. amethystina J.C.Mikan, P. campanulata Mast., P. capsularis L., P. edulis Sims, P. haematostigma Mart. ex Mast., P. marginata Mast., P. mediterranea Vell., P. porophylla Vell., P. sidifolia M.Roem., P. speciosa Gardner e uma espécie ainda não identificada, possivelmente tratando-se de um novo táxon. Para reconhecimento das espécies são apresentadas chave de identificação, descrições, ilustrações, distribuição geográfica e comentários taxonômicos das espécies

Comparison between static and semidynamic models for microcosm biofilm formation on dentin

Microcosm biofilm has been applied to induce carious lesions in dentin. However, no study has been done to compare the impact of the type of model for providing nutrients to microcosm biofilm formation on dentin. Objective: This study compared the performance of two kinds of models (static and semi-dynamic) on the biofilm formation and the development of dentin carious lesions. Material and Methods: In both models, biofilm was produced using inoculum from pooled human saliva mixed with McBain saliva for the first 8 h (5% CO2 and 370C). Afterwards, for the static model, the samples were placed in 24-wells microplate containing McBain saliva with 0.2% sucrose, which was replaced at 24 h. In the semi-dynamic model, the samples were submitted to artificial mouth system with continuous flow of McBain saliva with 0.2% sucrose (0.15 ml/min, 370C) for 10 h a day (for the other 14 h, no flow was applied, similarly to the static model). After 5 days, biofilm viability was measured by fluorescence and dentin demineralization by transverse microradiography. Results: Biofilm viability was significantly lower for the static compared with semi-dynamic model, while dentin demineralization was significantly higher for the first one (p<0.05). The static model was able to produce a higher number of typical subsurface lesions compared with the semi-dynamic model (p<0.05). Conclusions: The type of model (static and semi-dynamic) applied in the microcosm biofilm may have influence on it's viability and the severity/profile of dentin carious lesions

Educação médica online durante a Pandemia de COVID-19: relatos de experiência após mentoria/ Online medical education during the COVID-19 Pandemic: experience reports after mentorship

O distanciamento e isolamento social decorrente da pandemia causada pela doença coronavírus-2019 (COVID-19), trouxe grandes mudanças nas vidas dos estudantes de medicina do Brasil, incluindo a migração para o ensino à distância. Nesse contexto, durante o primeiro semestre do ano de 2020, cinco acadêmicos da Universidade Federal de Roraima (UFRR) foram submetidos à uma mentoria por vídeo conferência, dirigida por um médico e livre docente da Faculdade de Medicina da Universidade de São Paulo (FMUSP), em que foram explorados assuntos inerentes à formação médica, como gestão de tempo, organização dos estudos, iniciação científica, empatia, resiliência, educação financeira, carreira profissional e especialidades médicas. Este estudo foi realizado utilizando os relatos de experiências que foram produzidos pelos acadêmicos de medicina da UFRR após a mentoria por vídeo conferência. A maioria dos participantes relatou sentir que a pandemia de 2020 trouxe desafios de adaptação, diante da substituição do estudo presencial para a forma online, além de relatarem impactos positivos em relação às orientações abordadas pela mentoria. Na análise dos textos livres e subjetivos, foi possível observar um alto nível de satisfação dos alunos em relação à mudança para a aprendizagem online, com destaque às favoráveis de acessibilidade, a forma leve e descontraída das reuniões e a grande capacidade de gerar autorreflexão. O ensino médico à distância não substitui o presencial, todavia, como uma forma de auxílio e complementação, pode ser de grande ajuda na busca do conhecimento e aprimoramento na formação acadêmica

Investigando a relação formação médica e atividade física: significados, procedimentos e resultados

For many years, it has been issued classes of Physical Education to medical course as a regular activity. Considering the interest raised, the present communication introduces and discusses some aspects of this project. It is a didatic–methodologic process that encompasses sharing pratices and planned reflexions, based on i) pedagogical principles; ii) identifications of significant subjects to the students and iii) dialogue /rebuilding of these two conjuncts. Proceedings, results and meanings are presented and discussed with some detail.Por proporcionar a confluência de interesses comuns, a disciplina Educação Física Curricular ministrada para calouros do curso de Medicina, se identifica como projeto de ensino/pesquisa, interessante para ser conhecido e apreciado. Trata-se de processo didático-metodológico em que se aliam práticas e reflexões participativamente planejadas, baseadas em princípios pedagógicos problematizadores para a identificação de temáticas significativas aos alunos e na interlocução/ reconstrução destes dois conjuntos. Na presente comunicação, relatamse e discutem-se respectivos resultados e significados

Investigando a relação formação médica e atividade física: significados, procedimentos e resultados

For many years, it has been issued classes of Physical Education to medical course as a regular activity. Considering the interest raised, the present communication introduces and discusses some aspects of this project. It is a didatic–methodologic process that encompasses sharing pratices and planned reflexions, based on i) pedagogical principles; ii) identifications of significant subjects to the students and iii) dialogue /rebuilding of these two conjuncts. Proceedings, results and meanings are presented and discussed with some detail.Por proporcionar a confluência de interesses comuns, a disciplina Educação Física Curricular ministrada para calouros do curso de Medicina, se identifica como projeto de ensino/pesquisa, interessante para ser conhecido e apreciado. Trata-se de processo didático-metodológico em que se aliam práticas e reflexões participativamente planejadas, baseadas em princípios pedagógicos problematizadores para a identificação de temáticas significativas aos alunos e na interlocução/ reconstrução destes dois conjuntos. Na presente comunicação, relatamse e discutem-se respectivos resultados e significados

Effect of hydroalcoholic extract of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves on the viability and activity of microcosm biofilm and on enamel demineralization

Objectives: The aim of this study was to assess the effect of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves hydroalcoholic extracts on viability and metabolism of a microcosm biofilm and on enamel demineralization prevention. Methodology: Microcosm biofilm was produced on bovine enamel using inoculum from pooled human saliva mixed with McBain saliva, under 0.2% sucrose exposure, for 14 days. The biofilm was daily-treated with the extracts for 1 min. At the end, it was analyzed with respect to viability by fluorescence, CFU counting and extracellular polysaccharides (phenol-sulphuric acid colorimetric assay) and lactic acid (enzymatic assay) production. The demineralization was measured by TMR. The data were compared using ANOVA or Kruskal-Wallis (p<0.05). Results: M. urundeuva All. at 100, 10 and 0.1 μg/mL and Q. grandiflora Mart. at 100 and 0.1 μg/mL reduced biofilm viability similarly to positive control (chlorhexidine) and significantly more than the negative-vehicle control (35% ethanol). M. urundeuva at 1000, 100 and 0.1 μg/mL were able to reduce both lactobacilli and mutans streptococci CFU counting, while Q. grandiflora (1000 and 1.0 μg/mL) significantly reduced mutans streptococci CFU counting. On the other hand, the natural extracts were unable to significantly reduce extracellular polysaccharides and lactic acid productions neither the development of enamel carious lesions.  Conclusions: The extracts showed antimicrobial properties on microcosm biofilm, however, they had no effect on biofilm metabolism and caries protection

The interplay between HPV, other sexually transmissible infections and genital microbiome on cervical microenvironment (MicroCervixHPV study)

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The writing of this manuscript received the support of Fundação para a Ciência e a Tecnologia, Grant number UIDB/04295/2020, and doctoral scholarships (SFRH/BD/132900/2017). The authors acknowledge financial support from the Instituto de Investigação Científica Bento da Rocha Cabral, Clinical Analysis Laboratory Joaquim Chaves Saúde, Instituto de Saúde Ambiental, Faculdade de Medicina da Universidade de Lisboa and Escola Superior de Tecnologia de Saúde de Lisboa, Instituto Politécnico de Lisboa.Background: The importance of Cervicovaginal Microbiota in protecting against infections (such as HPV) is already well established, namely through Lactobacillus spp., as well as the mechanism through which HPV leads to Cervical Neoplasia. However, it is not possible to classify HPV as a complete carcinogen. Thus, the importance of exploring Cervicovaginal dysbiosis to decipher this interaction with HPV takes on greater relevance. The main objectives of this study were: 1) Comparison of the MCV composition of women with or without HPV and women with ASCUS or LSIL; 2) Characterization of cytokines present in the vaginal microenvironment; 3) Evaluation of the blood count ratios as prognostic systemic inflammatory biomarkers; 4) Correlation between MCV, HPV serotypes, and cytokines. Methods: This was a retrospective, observational, multicenter, cross-sectional study. CVM analysis was performed by isolation RNA and sequencing on a NGS platform. Cytokine concentrations of CVM were obtained through the Multiplex platform. Statistical analysis was performed in SPSS v 26.0. An α of 0.05 was considered statistically significant. Results: Highlighting the core of the study, CVM types of CST I and CST IV were found to influence the emergence of cervical lesions. The neutrophil-to-lymphocyte ratio was found to impact the prognosis of ASCUS. Within CVM, Lactobacillus prevents the growth of other CST IV species, while the latter express symbiotic relationships with each other and show affinity for specific HPV serotypes. At last, RANTES chemokine is significantly elevated in cervicovaginal infections. Conclusion: The importance of using vaginal cytokine profiles and CVM is highlighted in the hypothesis of prevention of Cervical Neoplasia development, as well as in its use as a prognostic biomarker. Taken together, these insights are one step closer to personalized medicine.info:eu-repo/semantics/publishedVersio

Protein hydrolysates from salmon heads and cape hake by-products: comparing enzymatic method with subcritical water extraction on bioactivity properties

Fish by-products can be converted into high-value-added products like fish protein hydrolysates (FPHs), which have high nutritional value and are rich in bioactive peptides with health benefits. This study aims to characterise FPHs derived from salmon heads (HPSs) and Cape hake trimmings (HPHs) using Alcalase for enzymatic hydrolysis and Subcritical Water Hydrolysis (SWH) as an alternative method. All hydrolysates demonstrated high protein content (70.4–88.7%), with the degree of hydrolysis (DH) ranging from 10.7 to 36.4%. The peptide profile of FPHs indicated the breakdown of proteins into small peptides. HPSs showed higher levels of glycine and proline, while HPHs had higher concentrations of glutamic acid, leucine, threonine, and phenylalanine. Similar elemental profiles were observed in both HPHs and HPSs, and the levels of Cd, Pb, and Hg were well below the legislated limits. Hydrolysates do not have a negative effect on cell metabolism and contribute to cell growth. HPSs and HPHs exhibited high 2,2′–azino-bis(3 ethylbenzthiazoline-6)-sulfonic acid (ABTS) radical scavenging activity, Cu2+ and Fe2+ chelating activities, and angiotensin-converting enzyme (ACE) inhibitory activity, with HPHs generally displaying higher activities. The α-amylase inhibition of both FPHs was relatively low. These results indicate that HPHs are a promising natural source of nutritional compounds and bioactive peptides, making them potential candidates for use as an ingredient in new food products or nutraceuticals. SWH at 250 °C is a viable alternative to enzymatic methods for producing FPHs from salmon heads with high antioxidant and chelating properties.info:eu-repo/semantics/publishedVersio

Análise de achados histeroscópicos em mulheres com espessamento endometrial à ultrassonografia transvaginal em um hospital público em Minas Gerais: um estudo observacional / Analysis of hysteroscopic findings in women with endometrial thickness seen in transvaginal ultrasonography seen in a public hospital in Minas Gerais: an observational study

A ultrassonografia transvaginal (USTV) é um exame muito utilizado na propedêutica ginecológica. Um achado de espessamento endometrial à USTV, por exemplo, requer maior investigação, geralmente realizada pela histeroscopia diagnóstica, o método mais apropriado para avaliar a cavidade uterina. Estabelecer o perfil epidemiológico da mulher com achados de espessamento endometrial à USTV e investigar a correlação entre os achados desse exame e os provenientes da histeroscopia. Estudo transversal e prospectivo com delineamento observacional, baseado na análise histeroscópica de 79 pacientes cuja USTV constata espessamento endometrial. Das pacientes que apresentavam espessamento endometrial, foi obtida uma média de idade de 55,9 ± 11,4 anos. Dentre essas, 52 (65,8%) estavam na pós-menopausa. 31 mulheres (39,24%) eram assintomáticas e 48 (60,75%) relataram queixas de sangramento uterino anormal e dor pélvica 31 (39,24%). À histeroscopia, apenas 18 pacientes (22,8%) apresentavam de fato espessamento endometrial, sendo que 46 (58,2%) apresentavam pólipo endometrial, 9 (11,4%) apresentavam mioma submucoso e 10 (12,7%) não possuíam alterações na cavidade uterina. Apenas 3 (3,8%) das pacientes apresentaram lesões sugestivas de neoplasia maligna. As pacientes abordadas no estudo eram mulheres pós-menopausa, multíparas, com IMC acima da faixa considerada adequada e alta prevalência de comorbidades, em especial a hipertensão arterial sistêmica. Além de espessamento endometrial à USTV, queixavam de sangramento uterino anormal e/ou dor pélvica. Devido a esses fatores, essas mulheres possuíam um maior risco para neoplasias endometriais, o que justifica o acompanhamento e investigação por meio da histeroscopia

Experimentos demonstrativos na forma de show: formas alternativas relacionadas ao ensino de química

Experiments in the form of show discloses the chemical informally from the theatrical language, arousing curiosity and stimulating students' interest in this area. Thus, the CIRQUIM, group of undergraduate students in chemistry at the Universidade Federal de Goiás – Campus Jataí, offers presentations in the form of theater by staging a classroom where there is change of attitude of the teacher, suggesting the realization of experiments at room class. The CIRQUIM has made over 40 presentations to elementary students and middle Goiás - GO, showing experiments with visual effects and aroused the interest of viewers.Experimentos demonstrativos na forma de show divulgam a química informalmente a partir da linguagem teatral, despertando a curiosidade e incentivando o interesse dos alunos por esta área. Dessa maneira, o CIRQUIM, grupo formado por alunos de licenciatura em Química da Universidade Federal de Goiás – Campus Jataí, propõe apresentações em forma de teatro, encenando uma aula em que há mudança de atitude da professora, sugerindo a realização de experimentos em sala de aula. O CIRQUIM já realizou mais de 40 apresentações para alunos do ensino fundamental e médio de Jataí – GO, mostrando experimentos com efeitos visuais e despertando o interesse dos espectadores