Hydraulic hazard mapping in alpine dam break prone areas: the Cancano dam case

Dam-break hazard assessment is of great importance in the Italian Alps, where a large number of medium and large reservoirs are present in valleys that are characterized by widespread urbanized zones on alluvial fans and along valley floors. Accordingly, there is the need to identify specific operative approaches in order to quantify hydraulic hazard which in mountain regions inevitably differ from the ones typically used in flat flood-prone areas. These approaches take advantage of: 1) specific numerical algorithms to pre-process the massive topographic information generally needed to describe very irregular bathymetries; 2) an appropriate mathematical model coupled with a robust numerical method which can deal in an effective way with variable geometries like the ones typical of natural alpine rivers; 3) suitable criteria for the hydraulic hazard assessment; 4) representative test cases to verify the accuracy of the overall procedure. This contribution presents some preliminary results obtained in the development of this complex toolkit, showing its application to the test case of the Cancano dam-break, for which the results from a physical model are available. This case was studied in 1943 by De Marchi, who investigated the consequences of the potential collapse of the Cancano dam in Northern Italy as a possible war target during the World War II. Although dated, the resulting report (De Marchi, 1945) is very interesting, since it mixes in a synergistic way theoretical, experimental and numerical considerations. In particular, the laboratory data set concerning the dam-break wave propagation along the valley between the Cancano dam and the village of Cepina provides an useful benchmark for testing the predictive effectiveness of mathematical and numerical models in mountain applications. Here we suggest an overall approach based on the 1D shallow water equations that proved particularly effective for studying dam-break wave propagation in alpine valleys, although this kind of problems is naturally subject to "substantial uncertainties and unavoidable arbitrarinesses" (translation from De Marchi, 1945). The equations are solved by means of a shock-capturing finite volume method involving the Pavia Flux Predictor (PFP) scheme proposed by Braschi and Gallati (1992). The comparison between numerical results and experimental data confirms that the mathematical model adopted is capable of capturing the main engineering aspects of the phenomenon modeled by De Marchi

Study of tributary inflows in Lake Iseo with a rotating physical model

The influence of Coriolis force on the currents of large lakes is well acknowledged; very few contributions, however, investigate this aspect in medium-size lakes where its relevance could be questionable. In order to study the area of influence of the two major tributary rivers in Lake Iseo, a rotating vertically distorted physical model of the northern part of this lake was prepared and used, respecting both Froude and Rossby similarity. The model has a horizontal length scale factor of 8000 and a vertical scale factor of 500 and was used both in homogeneous and in thermally stratified conditions. We explored the pattern of water circulation in front of the entrance mouth for dif- ferent hydrologic scenarios at the beginning of spring and in summer. We neglected the influence of winds. The primary purposes of the model were twofold: i) to increase our level of knowledge of the hydrodynamics of Lake Iseo by verifying the occurrence of dynamical effects related to the Earth’s rotation on the plume of the two tributaries that enter the northern part of the lake and ii) to identify the areas of the lake that can be directly influenced by the tributaries’ waters, in order to provide guidance on water quality monitoring in zones of relevant environmental and touristic value. The results of the physical model confirm the relevant role played by the Coriolis force in the northern part of the lake. Under ordinary flow conditions, the model shows a systematic deflection of the inflowing waters towards the western shore of the lake. The inflow triggers a clockwise gyre within the Lovere bay, to the West of the inflow, and a slow counter-clockwise gyre, to the East of the inflow, that returns water towards the river mouth along the eastern shore. For discharges with higher return period, when only the contribution by Oglio River is relevant, the effect of the Earth’s rotation weakens in the entrance zone and the plume has a more rectilinear pattern, whilst in the far field the current driven by the inflows keeps moving along the western shore. On the basis of these results one could expect that the north-western part of the lake between Castro and Lovere, although not aligned with the tributaries’ axes, is more sensitive to accumulation effects related to river-borne pollution. The results obtained with the physical model are critically compared with data obtained from different sources: the trajectory of a lagrangian drogue; a map of reflectivity data from the lake floor; a map of water turbidity at the intrusion depth. The findings are also confirmed by the results of a 3D numerical model of the lake

Nutrient delivery efficiency of a combined sewer along a lake challenged by incipient eutrophication

Although sewage diversion outside of a lake's watershed is now ordinary practice in the restoration of eutrophic lakes, often the observed recovery is slower than expected and the internal load from the lake anoxic sediments is identified as a possible reason. However, in the case of combined sewer, the quantification of the residual nutrient load discharged from sewer spillways must also be questioned. In this paper, the diversion efficiency of the sewer system along the east coast of Lake Iseo, a prealpine Italian lake where eutrophication effects are still severe, is investigated. To this purpose, a representative part of the sewer system was modelled by PCSWMM and calibrated by using an extensive series of discharge measurements. Water quality monitoring during wet weather periods reveals that the first flush is common in tributary sewers, whereas it is absent along the main collector. Moreover, flow discharges are strongly affected by infiltration waters, which are controlled by the lake water level. The calibrated model, including infiltration modeling, was used to assess the annual overflow volumes and the nutrient load through a continuous 10-year simulation. Simulations were conducted both with regard to the current conditions and to a climate change scenario. Results show that the discharged residual load is at least 7 times larger than the design value, with the water infiltration contributing to 17% to the overflow volume and that non-structural practices could considerably reduce the overall impact of the sewer. This research thus provides important insight into the potential impact of combined sewer overflows on lacustrine environments and addresses effective mitigation measures in similar contexts

Esthesioneuroblastoma is not a member of the primitive peripheral neuroectodermal tumour-Ewing’s group

Esthesioneuroblastoma (ENB) is a rare, site-specific, locally aggressive neuronal malignancy so far thought to belong to primitive peripheral neuroectodermal tumour-Ewing's tumour (pPNETs-ETs). Its anatomical location, in addition to morphologic, immunophenotypic and ultrastructural features, suggests its origin in the neuronal or neuroendocrine cells of the olfactory epithelium. However, the cytogenetic and molecular data currently available appear controversial on the presence of the typical translocation t(11;22)(q24;q12) and of trisomy 8, chromosomal changes that characterize the tumours belonging to the pPNETs-ETs. Herein we have analysed five ENB tumour specimens for trisomy 8 by fluorescence in situ hybridization (FISH), for the presence of EWS gene rearrangements by FISH, reverse transcription polymerase chain reaction and Southern blot analyses, as well as for the expression of the Ewing sarcoma-associated MIC2 antigen by immunohistochemistry. Neither EWS/FLI-I, EWS/ERG and EWS/FEV fusion genes nor MIC2 expression were found in any tumour, whereas trisomy 8 was found in one case only. Moreover, DNA from three cases analysed by Southern blot did not show EWS gene rearrangements. Our results support the evidence that ENB is not a member of the pPNETs-ETs. © 1999 Cancer Research Campaig

Following basal stem rot in young oil palm plantings

The PCR primer GanET has previously been shown to be suitable for the specific amplification of DNA from Ganoderma boninense. A DNA extraction and PCR method has been developed that allows for the amplification of the G. boninense DNA from environmental samples of oil palm tissue. The GanET primer reaction was used in conjunction with a palm-sampling programme to investigate the possible infection of young palms through cut frond base surfaces. Ganoderma DNA was detected in frond base material at a greater frequency than would be expected by comparison with current infection levels. Comparisons are made between the height of the frond base infected, the number of frond bases infected, and subsequent development of basal stem rot. The preliminary results suggest that the development of basal stem rot may be more likely to occur when young lower frond bases are infected

The preparation of the Shutdown Dose Rate experiment for the next JET Deuterium-Tritium campaign

The assessment of the Shutdown Dose Rate (SDR) due to neutron activation is a major safety issue for fusion devices and in the last decade several benchmark experiments have been conducted at JET during Deuterium-Deuterium experiments for the validation of the numerical tools used in ITER nuclear analyses. The future Deuterium-Tritium campaign at JET (DTE2) will provide a unique opportunity to validate the codes under ITER-relevant conditions through the comparison between numerical predictions and measured quantities (C/E). For this purpose, a novel SDR experiment, described in the present work, is in preparation in the frame of the WPJET3-NEXP subproject within EUROfusion Consortium. The experimental setup has been accurately designed to reduce measurement uncertainties; spherical air-vented ionization chambers (ICs) will be used for on-line ex-vessel decay gamma dose measurements during JET shutdown following DT operations and activation foils have been selected for measuring the neutron fluence near ICs during operations. Active dosimeters (based on ICs) have been calibrated over a broad energy range (from about 30 keV to 1.3 MeV) with X and gamma reference beam qualities. Neutron irradiation tests confirmed the capability of active dosimeters of performing on-line decay gamma dose rate measurements, to follow gamma dose decay at the end of neutron irradiation as well as insignificant activation of the ICs

A Panel of Eight miRNAs Is Deregulated in HTLV-2 Infected PBMCs and BJABGu Cell Line

Despite human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 being retroviruses closely related at a genomic level, HTLV-2 differs from HTLV-1 in terms of pathogenicity in both single infection and coinfection contexts. Moreover, the HTLV-2 association with clinical outcomes is still debated and several mechanisms underlying HTLV-2 infection remain unexplored as well. Cellular miRNAs are key factors in the post-transcriptional regulation of gene expression and they are known to be potential targets for several pathogens to control the host microenvironment and, in particular, escape immune responses. Here, we identified a HTLV-2-related signature of eight miRNAs (miR-125a-3p, miR-381-3p, miR-502-5p, miR-708-5p, miR-548d-5p, miR-548c-5p, miR-1-3p, and miR-511-5p) in both HTLV-2 infected PBMC and BJABGu cell lines. Altered miRNA expression patterns were correlated with the impairment of Th cell differentiation and signaling pathways driven by cytokines and transcriptional factors such as the Runt-related transcription factor (RUNX) family members. Specifically, we demonstrated that the RUNX2 protein was significantly more expressed in the presence of Tax-2 compared with Tax-1 in an in vitro cell model. To the best of our knowledge, these data represent the first contribution to elucidating the HTLV-2 mediated alteration of host cell miRNA profiles that may impact on HTLV-2 replication and persistent infection

Identification of a novel spliced variant of the SYT gene expressed in normal tissues and in synovial sarcoma

Synovial sarcoma (SS) is cytogenetically characterized by the translocation t(X;18)(p11.2-q11.2) generating a fusion between the SYT gene on chromosome 18 and one member of the SSX family gene (SSX1; SSX2; SSX4) on chromosome X. Here, we report for the first time that 2 forms of SYT mRNA are present in both normal tissues and SSs. By amplifying the full-length SYT cDNA of two SSs, we detected 2 bands, here designated N-SYT and I-SYT. The latter, previously undescribed, contains an in-frame insertion of 93 bp. Its sequencing revealed a 100% homology with the mouse SYT gene. These two SYT forms were present, although in different amounts, in all human normal tissues examined, including kidney, stomach, lung, colon, liver and synovia. Coexistence of N-SYT and I-SYT (both fused with SSX) was detected in a series of 59 SSs (35 monophasic and 24 biphasic) and in a SS cell line. A preliminary analysis of the differential expression levels of N-SYT and I-SYT in SSs revealed that the latter was consistently overexpressed, suggesting a role in SS pathogenesis. © 2001 Cancer Research Campaign http://www.bjcancer.co