1,173 research outputs found
Las fronteras de la publicidad televisada en la era de conexión
The reflection considers the boundaries between the Internet and televised advertising and has as a theoretical and methodological approach the Semiotics of Culture, with the discussion based on the concept of boundary proposed by Lotman (1999) and the incursions of the authors Jenkins et al. . (2014) and Manovich (2005) on the era of the connection of culture, as well as the notes of Machado A. (2011) on the audiovisual. We care about the dialogue and the intersections between the different sign systems, because we believe that just as the Internet transforms the way advertising is produced, at the same time the internet also overlaps with it.la reflexión piensa las fronteras entre la internet y la publicidad televisada y tiene como enfoque teórico y metodológico la Semiótica de la Cultura, con la discusión basada en el concepto de frontera propuesto por Lotman (1999) y las incursiones de los autores Jenkins et al. (2014) y Manovich (2005) sobre la era de la conexión de la cultura, así como los apuntes de Machado A. (2011) sobre el audiovisual. Nos importa el diálogo y las intersecciones entre los diferentes sistemas de signos, pues creemos que así como la internet transforma la manera de producir la publicidad, al mismo tiempo se sobrepone a ella.la reflexión piensa las fronteras entre la internet y la publicidad televisada y tiene como enfoque teórico y metodológico la Semiótica de la Cultura, con la discusión basada en el concepto de frontera propuesto por Lotman (1999) y las incursiones de los autores Jenkins et al. (2014) y Manovich (2005) sobre la era de la conexión de la cultura, así como los apuntes de Machado A. (2011) sobre el audiovisual. Nos importa el diálogo y las intersecciones entre los diferentes sistemas de signos, pues creemos que así como la internet transforma la manera de producir la publicidad, al mismo tiempo se sobrepone a ella
Quantificação dos furanoeliangolidos por HPLC e CG
Neste trabalho são descritos o desenvolvimento e comparação de dois métodos analíticos (CLAE e CG) para quantificação dos furanoeliangolidos mais comuns em Lychnophora.Ambos os métodos são sensíveis e adequados para a quantificação desses metabólitos.The development and comparison of two analytical methods (HPLC and GC) for the quantification of the most common furanoheliangolides from Lychnophora is reported in this paper. Both methods are sensitive and suitable for quantification of these metabolites
Exploring Cheminformatic Toolsets for Predicting the Dermal Toxicity of Furanocoumarins
Linear furanocoumarins are skin sensitizers and anticancer agents whose appeal in skincare therapeutics is widely exploited. Owing to the need to predict the biological activities of medicines, this work aimed the investigate the predicted dermal toxicity of linear furanocoumarins through chemoinformatic approaches. Therefore, eight major linear furanocoumarins of interest in medicine were selected, and their pharmacophores / toxicophores were modelled and inputted in several databases and cheminformatic toolsets previously described in the literature. Moreover, Principal Components Analysis was performed to allow multivariable comparisons. Results showcased that the first two PCs accounted for 95.48% of all variance in the model, and molecular weight and polar surface showcased a positive correlation to Log P and Log Kp, which may be involved in skin penetration. Moreover, the pharmacophore modelling evidenced superimposition between linear furanocoumarins, ethidium bromide and acridine orange, thereby suggesting that these compounds share similar biological effects, supported by their acknowledged DNA intercalating activities. Therefore, this work showcased the application of various cheminformatic tools to screen the dermal toxicity of chemicals
ANÁLISE FITOQUÍMICA E DOSEAMENTO DE METABÓLITOS SECUNDÁRIOS DAS FOLHAS DE Miconia albicans (Sw) TRIANA COLETADAS DE DUAS REGIÕES DO ESTADO DE GOIÁS
Introdução: Uma espécie medicinal que está sendo utilizada pela população e que possui poucos estudos científicos na literatura relacionados à análise fitoquímica, bem como variação quantitativa de metabólitos frente a nutrientes disponíveis no solo é a Miconia albicans (Sw) Triana, conhecida popularmente como canela de velho. Objetivo: Determinar o perfil fitoquímico e quantificar os principais metabólitos secundários das folhas de M. albicans coletadas em duas regiões distintas. Metodologia: As folhas de M. albicans foram coletadas no município de Niquelândia-GO e Nova Glória-GO. Posteriormente, as folhas foram secas e trituradas, e realizadas as seguintes análises: prospecção fitoquímica, quantificação de compostos fenólicos, taninos e flavonoides e composição (macro e micronutrientes) do solo entre as duas regiões. Resultados e discussão: As duas amostras, por meio dos ensaios de fitoquímica, apresentaram positivas para flavonoides, saponinas, cumarinas e taninos. Quanto a quantificação de metabólitos, a amostra coletada na região de Niquelândia-GO apresentou maior percentual significativo de compostos fenólicos (29,20 %) e taninos (24,52 %) comparada com a amostra coletada no município de Nova Glória-GO, provavelmente devido à baixa quantidade de minerais e elevada toxidez por alumínio do solo.Conclusão: As folhas de M. albicans coletadas nas duas regiões distintas possuem flavonoides, saponinas, cumarinas e taninos como metabólitos secundários, os quais são responsáveis pelos efeitos antioxidantes, antiinflamatórios, analgésicos e antimicrobianos citados pela população. E a composição do solo, onde o vegetal foi desenvolvido, interfere na produção de metabólitos secundários.Nutriente
Laboratorial evaluation of hypertonic and isotonic saline solution and furosemide use for the ammonia poisoning treatment in cattle
Para testar a eficiência de vários tratamentos de intoxicação por amônia em bovinos, foram utilizados 25 garrotes que receberam cloreto de amônio por infusão intravenosa (iv) até o surgimento de quadro convulsivo. Em seguida, os animais foram alocados em um dos cinco grupos experimentais e tratados da seguinte forma: 1) controle: infusão (iv) de 300mL de solução salina isotônica (SSI), no decorrer de 4h; 2) infusão (iv) de 30mL kg-1 PV de SSI no decorrer de 4h e administração de 4L de água intraruminal por meio de sonda esofágica (ASE); 3) mesmo tratamento do grupo 2 e dose única (iv) de furosemida (2mg kg-1 PV) (F); 4) injeção (iv) de 5mL kg-1 PV de solução salina hipertônica (SSH) 7,2% nos primeiros 30min, seguida de 20mL kg-1 PV de SSI e 4L de ASE; 5) mesmo tratamento do grupo 4 e dose única de F. No decorrer de 4h após a convulsão, foram determinados os teores plasmáticos de amônia e glicose, ureia, creatinina, potássio e sódio séricos, volume e gravidade específica da urina, e excreção urinária de amônio e ureia. No momento da convulsão, os teores de amônia plasmáticos foram muito altos e idênticos em todos os tratamentos, mas no 120°min, nos grupos tratados com associação de SSH+SSI+ASE (grupos 4 e 5), houve diminuição desse metabólito. O uso de furosemida (grupos 3 e 5) não aumentou a excreção total de urina. A terapia com associação de SSH+SSI+ASE aumentou ainda o volume urinário e a excreção percentual urinária de ureia e amônia durante o período crítico da 1a hora de tratamento, mas o uso de SSI+ASE (grupos 2 e 3) teve resultados intermediários. A eficiência do tratamento com SSH+SSI+ASE ou SSI+ASE foi superior ao grupo controle. Embora com efeito menor que o observado com SSH+SSI+ASE, a SSI+ASE promoveu melhora no quadro clínico geral e, ao término do experimento, promoveu também uma adequada desintoxicação da amônia.To test the efficiency of some treatments for ammonia poisoning in cattle, 25 steers were used. Ammonium chloride solution was infused intravenously (iv) in each steer until the onset of convulsive episode. Thereafter, the animals were distributed in one of the five different groups, as follows: 1) control: infusion (iv) of 300mL isotonic saline solution (ISS) throughout the following 4h; 2) infusion (iv) of ISS 30mL kg-1 BW throughout the following 4h and administration of 4L water (W) through stomach tube; 3) the same as group 2, plus a single dose (iv) 2g furosemide kg-1 BW (F); 4) injection of 5mL kg-1 BW hypertonic saline solution (HSS) (7.2%) (iv) throughout the first 30min, followed by 20mL kg-1 BW of ISS and 4L W.; 5) the same as group 4 and a single dose of F. For the next 4h after the convulsion, plasma concentration of ammonia and glucose, serum urea, creatinine, sodium and potassium, urine total volume and specific gravity, and urinary excretion of ammonium and urea were followed. At the convulsion, the ammonia blood levels were high and similar among the groups, but at the 120th min the animals treated with HSS+ISS+W (groups 4 and 5) had lower values than the control. Furosemide (groups 3 and 5) did not prevent an increase in the total excretion of urine. The therapy with HSS+ISS+W still increased the urinary volume and the total urinary excretion of urea and ammonium during the critical 1st h of treatment, while the use of ISS+W had intermediate results. The efficiency of the treatment with HSS+ISS+W or ISS+W was better than the control group. Although with lower efficiency as seen in the treatment with HSS+ISS+W, the ISS+W caused an improvement in the clinical picture and at the end of the experiment generated an adequate ammonia detoxificatio
Contributions to nonlinear elliptic equations and systems: a tribute to Djairo Guedes de Figueiredo on the occasion of his 80th birthday
This volume of contributions pays tribute to the life and work of Djairo Guedes de Figueiredo on the occasion of his 80th birthday. The articles it contains were born out of the ICMC Summer Meeting on Differential Equations – 2014 Chapter, also dedicated to de Figueiredo and held at the Universidade de São Paulo at São Carlos, Brazil from February 3-7, 2014. The contributing authors represent a group of international experts in the field and discuss recent trends and new directions in nonlinear elliptic partial differential equations and systems. Djairo Guedes de Figueiredo has had a very active scientific career, publishing 29 monographs and over one hundred research articles. His influence on Brazilian mathematics has made him one of the pillars of the subject in that country. He had a major impact on the development of analysis, especially in its application to nonlinear elliptic partial differential equations and systems throughout the entire world. The articles collected here pay tribute to him and his legacy, and are intended for graduate students and researchers in mathematics and related areas who are interested in nonlinear elliptic partial differential equations and systems
EXPERIÊNCIA E PARAMETRIZAÇÃO NO PROCESSO DE PROJETO DE APLICAÇÕES DIGITAIS INTERATIVAS
Designers’ experiences are reflected on prototypes developed by them, which are of paramount importance to the formation of concepts, especially in the design and design of computer systems phases. Therefore, this article demonstrates how prototyping can benefit from the parameter in the design of these systems. To make it happen, the design process, exposed in this article, is established methodologically by the understanding of concepts that spread through the experiential and interactive contexts (immersion), in the structuring of ideas (ideation) that delineate one solution liable to implementation (prototype) and its subsequent validation. As a result, a computational solution that consists of an embedded system is proposed, validated by functional tests that show that parametric modeling hold up the establishment of relations and correlations between the components used in prototyping, and demonstrate that the parameter assists both the creation and the redesign of interactive computer systems.Experiências de projetistas se retratam nos protótipos por eles desenvolvidos, os quais são de suma importância para a formação de conceitos, principalmente na fase de projeto e de concepção de sistemas computacionais. Diante disso, o presente artigo tem como objetivo demonstrar como a prototipação pode se beneficiar da parametrização na concepção destes sistemas. Para isso, o processo projetual, exposto neste artigo, é estabelecido metodologicamente pelo entendimento de conceitos que permeiam os contextos experienciais e interacionais (imersão); na estruturação de ideias (ideação) que delineiam uma solução passível de implementação (prototipação) e na sua posterior validação. Como resultado, é proposta uma solução computacional constituída por um sistema embarcado e validada por testes funcionais que retratam que a modelagem paramétrica favorece o estabelecimento de relações e correlações entre os componentes utilizados na prototipação, e demonstram que a parametrização auxilia tanto na criação quanto no redesenho de sistemas computacionais interativos
The sub-supersolution method for Kirchhoff systems: applications
In this paper we prove that the sub-supersolution method works for general
Kirchhoff systems. We apply the cited method to prove the existence of positive solutions for some specific models
Antileishmanial activity of extracts from Libidibia ferrea : development of in vitro and in vivo tests
Treatment of cutaneous leishmaniasis (CL) is difficult due to the scarce number of drugs able to eliminate completely the intracellular form of the parasite. In the present study, the aim was to evaluate: i) phytochemical properties of extracts from Libidibia ferrea; ii) antileishmanial activity of extracts from L. ferrea against promastigotes and amastigotes of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis; iii) the effects of topical treatment using hydrogel containing active extract of L. ferrea on golden hamsters infected with L. (L.) amazonensis. Extracts from leaves, branches and fruits of L. ferrea were obtained with hexane and methanol and were tested by in vitro assays in promastigotes and murine macrophages J774 experimentally infected with amastigotes of Leishmania. Groups of hamsters with CL received topical treatment with a formulation of extract (10%) hydrogels, 50 mg. day(-1) for 40 days. In vitro activity of FrMeOH (methanolic extract from fruits without seeds) resulted in significant reduction of viable promastigotes of L. (L.) amazonensis (IC50 of 15.4 mu g. mL(-1)) and demonstrated inhibition potential of amastigote forms of L. (L.) amazonensis and L. (V.) guyanensis and low cytotoxicity in macrophages. The overall data of topical treatment with extract hydrogels (GelFrMeOH) showed that lesion sizes were significantly reduced (42.78%), with low parasite burden by RT-qPCR and culture analysis by microscopy examination, and with histopathological findings such as lower inflammatory cell infiltration 40 days after treatment. Chemical analysis demonstrated FrMeOH contains high levels of phenolic compounds. The results indicate a possible alternative therapy for CL using phytotherapics. KEYWORDS: cutaneous leishmaniasis, natural products, topical formulations, experimental treatmentPeer reviewe
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