Evolution of the upper proterozoic continental deposits from «La Serena», (Southern Central Iberian Zone) Badajoz, Spain

[Resumen] Se describen por primera vez afloramientos, recientemente descubiertos, del Alcudiense Superior, en «La Serena», Badajoz. Se demuestra el carácter continental de la parte basal de varias secuencias, y se esboza un esquema paleogeográfico, que implica una línea de,costa hacia el Oeste, y la diferenciación de tres ciclos sedimentarios.[Abstract] Sorne recently discovered Upper Alcudian outcrops, from «La Serena» region in Badajoz province, are described in this paper for the first time. The basal pan of most of the studied logs shows continental characteristics here depicted; a paleogeographical scheme is also submitted. This latter implies moving the suggested coast line westward for the first cycle. Three local sedimentary cycles are here proposed and correlated with those from well known areas

Diagnóstico ecocardiográfico en equinos fina sangre de carrera

Echocardiographic examination is a fundamental part of cardiovascular exam if they want to evaluate the real connotation in the presentation of heart murmurs and / or arrhythmias and also where you want to evaluate the performance or the state in which it is related to process of adaptation of cardiovascular system to exercise. The horses are used right and left parasternal windows in which the transducer is moved between the 3rd and 5th intercostal space. In the right parasternal window is where you get more information and it can cut the heart in short axis and long-axis. In the long axis obtained from the right parasternal get the picture of four chambers (biventricular inflow tract), 5 chamber and left outflow tract. In the image of 4 cameras are 2 atrioventricular valves, that is to say, the tricuspid and mitral valves. In the image of the outflow tract of the left ventricle, we'll see right ventricle, right atrium, interventricular septum, left ventricle, sigmoid aortic valve and the aorta. In the image of 5 cameras the right ventricle, right atrium, left atrium, left ventricle and the aorta can be observed. Sometimes a picture is a round hypoechoic near the aorta corresponding to the pulmonary artery. Adaptive changes induced by training translate into changes in the heart that is evident on echocardiography. These changes are caused by increased volume at the end of diastole and increased cardiac output. The changes that can be evidenced by ultrasound are: Increase of cardiac mass, suggesting cardiac hypertrophy, mainly the left ventricle in trained horses. It is also suggested increasing the size of the cavity of the left ventricle and left atrium without changes in ventricular wall thickness, which may reflect the different types of training.    El examen ecocardiográfico es una parte fundamental del examen cardiovascular si se quiere evaluar la real connotación que pudiesen tener murmullos cardiacos y/o arritmias como también en aquellos casos que se desea evaluar el rendimiento deportivo ó el estado en el cual se encuentra éste en relación al proceso de adaptación del sistema cardiovascular al ejercicio. En el equino se usan las ventanas paraesternales derecha e izquierda, en las cuales se mueve el transductor entre el 3º y 5º espacio intercostal. Por la ventana paraesternal derecha es por donde se obtiene mayor informacion y en ella se puede realizar el corte del corazón en el eje corto y también en el eje largo. En el eje largo obtenido desde la ventana paraesternal derecha se obtiene la imagen de cuatro cámaras (tracto de entrada biventricular), 5 cámaras y tracto de salida izquierdo. En la imagen de 4 cámaras se ven 2 válvulas atrioventriculares, es decir, la tricúspide y la mitral. En la imagen del tracto de salida del ventrículo izquierdo, vamos a ver ventrículo derecho, atrio derecha, tabique interventricular, ventrículo izquierdo, válvula sigmoidea aórtica y la aorta. En la imagen de 5 cámaras se aprecia ventrículo derecho, atrio derecho, atrio izquierdo, ventrículo izquierdo y también se puede observar la aorta. Hay veces que se ve una imagen hipoecogénica de forma redondeada cercana a la aorta que corresponde a la arteria pulmonar. Los cambios adaptativos inducidos por el entrenamiento se traducen e cambios en el corazón que se evidencian en la ecocardiografía. Estos cambios son producidos por aumento del volumen al final de la diástole y aumento del debito cardiaco. Los cambios que se pueden evidenciar mediante ecografía son: Aumento de la masa cardiaca, sugiriendo hipertrofia cardiaca, principalmente del ventrículo izquierdo, en caballos entrenados. También se ha sugerido el aumento del tamaño de la cavidad del ventrículo izquierdo y del atrio izquierdo pero sin cambios en el grosor de la pared ventricular, lo que podría reflejar los diferentes tipos de entrenamiento.  

The echocardiography diagnosis of cor pulmonale in a horse

The authors describe the two-dimensional and Doppler signs that characterize the pulmonary hypertension caused by chronic obstructive pulmonary disease (COPD) in a horse

Hidrogeología del Karst de Pico Frentes (Cordillera Ibérica, España)

The karst system of Pico Frentes has developed within an Upper Cretaceous calcareous series whose well-defined folded geometry determines that its aquifer reserves are held mainly in three hydraulically-connected synclines, with a groundwater capacity of between 5 and 7 hm3 . The recharge to this unconfined peneplain aquifer is autogenous and diffuse. On a large scale, groundwater flow is directed by the base of the synclines, while on a small scale, it flows along groundwater conduits towards the Fuentetoba Spring (210 l/s) and source of the River Mazos (50 l/s), following a highly variable flow regime of low inertia, with other smaller discharges emanating during periods of high water. Analysis of hydrographs of these springs indicates a very variable rate system and little power regulating natural, characteristic of a typical karstic aquifer, with great capacity for renewal and low residence time. Using hydrogram simulations of these upwellings using a mathematical rainfall-runoff model, a detailed quantification of the average water balance was made for a twenty-year time series. This water balance consists of 16,86 hm3 rainfall (100%); natural recharge, 8,35 hm3 (49,53%); EVT 8,50 hm3 (50,41%); pumped groundwater abstractions, 0,01hm3 (0,06%); surface runoff, 0 hm3 , groundwater transfers to other aquifer, 0 hm3 .El sistema kárstico de Pico Frentes se ha desarrollado a favor de un conjunto calcáreo del Cretácico Superior cuya geometría plegada muy bien definida ha condicionado que los acuíferos se sitúen principalmente en tres sinclinales hidráulicamente conectados, con una capacidad de reservas subterráneas de entre 5 y 7 hm3 . La recarga en este acuífero libre y en penillanura es autógena y difusa. El flujo subterráneo va dirigido a gran escala por el fondo de los sinclinales y a pequeña escala mediante corrientes subterráneas hacia los manantiales de Fuentetoba (210 l/s) y nacimiento del rio Mazos (50 l/s), surgiendo en aguas altas otras descargas menores. El análisis de los hidrogramas de estos manantiales indica un sistema de régimen muy variable y poco poder de regulación natural, característica de un acuífero típicamente kárstico, con gran capacidad de renovación y poco tiempo de residencia. Gracias a la simulación de los hidrogramas de estas surgencias mediante un modelo matemático de precipitación –escorrentía, se ha cuantificado de manera detallada el balance hidráulico medio para una serie de 20 años: aportación pluviométrica 16,86 hm3 (100%), recarga natural 8,35 hm3 (49,53%), EVT 8,50 hm3 (50,41%), bombeo de agua subterránea 0,01hm3 (0,06%), escorrentía superficial 0 hm3 , transferencias subterráneas a otros acuíferos 0 hm3

Singularity resolution depends on the clock

We study the quantum cosmology of a flat Friedmann–Lemaître–Robertson–Walker Universe filled with a (free) massless scalar field and a perfect fluid that represents radiation or a cosmological constant whose value is not fixed by the action, as in unimodular gravity. We study two versions of the quantum theory: the first is based on a time coordinate conjugate to the radiation/dark energy matter component, i.e., conformal time (for radiation) or unimodular time. As shown by Gryb and Thébault, this quantum theory achieves a type of singularity resolution; we illustrate this and other properties of this theory. The theory is then contrasted with a second type of quantisation in which the logarithm of the scale factor serves as time, which has been studied in the context of the 'perfect bounce' for quantum cosmology. Unlike the first quantum theory, the second one contains semiclassical states that follow classical trajectories and evolve into the singularity without obstruction, thus showing no singularity resolution. We discuss how a complex scale factor best describes the semiclassical dynamics. This cosmological model serves as an illustration of the problem of time in quantum cosmology

Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures. © 2016, Centers for Disease Control and Prevention (CDC). All rights reserved

The Promoter of the Cereal VERNALIZATION1 Gene Is Sufficient for Transcriptional Induction by Prolonged Cold

The VERNALIZATION1 (VRN1) gene of temperate cereals is transcriptionally activated by prolonged cold during winter (vernalization) to promote flowering. To investigate the mechanisms controlling induction of VRN1 by prolonged cold, different regions of the VRN1 gene were fused to the GREEN FLUORESCENT PROTEIN (GFP) reporter and expression of the resulting gene constructs was assayed in transgenic barley (Hordeum vulgare). A 2 kb segment of the promoter of VRN1 was sufficient for GFP expression in the leaves and shoot apex of transgenic barley plants. Fluorescence increased at the shoot apex prior to inflorescence initiation and was subsequently maintained in the developing inflorescence. The promoter was also sufficient for low-temperature induction of GFP expression. A naturally occurring insertion in the proximal promoter, which is associated with elevated VRN1 expression and early flowering in some spring wheats, did not abolish induction of VRN1 transcription by prolonged cold, however. A translational fusion of the promoter and transcribed regions of VRN1 to GFP, VRN1::GFP, was localised to nuclei of cells at the shoot apex of transgenic barley plants. The distribution of VRN1::GFP at the shoot apex was similar to the expression pattern of the VRN1 promoter-GFP reporter gene. Fluorescence from the VRN1::GFP fusion protein increased in the developing leaves after prolonged cold treatment. These observations suggest that the promoter of VRN1 is targeted by mechanisms that trigger vernalization-induced flowering in economically important temperate cereal crops

Genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat (Triticum aestivum L.)

<p>Abstract</p> <p>Background</p> <p>To identify the genes involved in the development of low temperature (LT) tolerance in hexaploid wheat, we examined the global changes in expression in response to cold of the 55,052 potentially unique genes represented in the Affymetrix Wheat Genome microarray. We compared the expression of genes in winter-habit (winter Norstar and winter Manitou) and spring-habit (spring Manitou and spring Norstar)) cultivars, wherein the locus for the vernalization gene <it>Vrn-A1 </it>was swapped between the parental winter Norstar and spring Manitou in the derived near-isogenic lines winter Manitou and spring Norstar. Global expression of genes in the crowns of 3-leaf stage plants cold-acclimated at 6°C for 0, 2, 14, 21, 38, 42, 56 and 70 days was examined.</p> <p>Results</p> <p>Analysis of variance of gene expression separated the samples by genetic background and by the developmental stage before or after vernalization saturation was reached. Using gene-specific ANOVA we identified 12,901 genes (at <it>p </it>< 0.001) that change in expression with respect to both genotype and the duration of cold-treatment. We examined in more detail a subset of these genes (2,771) where expression was highly influenced by the interaction between these two main factors. Functional assignments using GO annotations showed that genes involved in transport, oxidation-reduction, and stress response were highly represented. Clustering based on the pattern of transcript accumulation identified genes that were up or down-regulated by cold-treatment. Our data indicate that the cold-sensitive lines can up-regulate known cold-responsive genes comparable to that of cold-hardy lines. The levels of expression of these genes were highly influenced by the initial rate and the duration of the gene's response to cold. We show that the <it>Vrn-A1 </it>locus controls the duration of gene expression but not its initial rate of response to cold treatment. Furthermore, we provide evidence that <it>Ta.Vrn-A1 </it>and <it>Ta.Vrt1 </it>originally hypothesized to encode for the same gene showed different patterns of expression and therefore are distinct.</p> <p>Conclusion</p> <p>This study provides novel insight into the underlying mechanisms that regulate the expression of cold-responsive genes in wheat. The results support the developmental model of LT tolerance gene regulation and demonstrate the complex genotype by environment interactions that determine LT adaptation in winter annual cereals.</p

Genetic basis of the very short life cycle of ‘Apogee’ wheat

Background: ‘Apogee’ has a very short life cycle among wheat cultivars (flowering 25 days after planting under a long day and without vernalization), and it is a unique genetic material that can be used to accelerate cycling breeding lines. However, little is known about the genetic basis of the super-short life of Apogee wheat. Results: In this study, Apogee was crossed with a strong winter wheat cultivar ‘Overland’, and 858 F2 plants were generated and tested in a greenhouse under constant warm temperature and long days. Apogee wheat was found to have the early alleles for four flowering time genes, which were ranked in the order of vrn-A1 \u3e VRN-B1 \u3e vrn- D3 \u3e PPD-D1 according to their effect intensity. All these Apogee alleles for early flowering showed complete or partial dominance effects in the F2 population. Surprisingly, Apogee was found to have the same alleles at vrn-A1a and vrn-D3a for early flowering as observed in winter wheat cultivar ‘Jagger.’ It was also found that the vrn-A1a gene was epistatic to VRN-B1 and vrn-D3. The dominant vrn-D3a alone was not sufficient to cause the transition from vegetative to reproductive development in winter plants without vernalization but was able to accelerate flowering in those plants that carry the vrn-A1a or Vrn-B1 alleles. The genetic effects of the vernalization and photoperiod genes were validated in Apogee x Overland F3 populations. Conclusion: VRN-A1, VRN-B1, VRN-D3, and PPD-D1 are the major genes that enabled Apogee to produce the very short life cycle. This study greatly advanced the molecular understanding of the multiple flowering genes under different genetic backgrounds and provided useful molecular tools that can be used to accelerate winter wheat breeding schemes