Cucumis metuliferus es resistente a poblaciones de Meloidogyne spp. incluso virulentas al gen MI

Se realizaron diversos ensayos para determinar la respuesta de C. metuliferus frente diversas poblaciones de Meloidogyne arenaria, M. incognita y M. javanica, algunas de las cuales habían sido ya caracterizadas como virulentas al gen de resistencia Mien tomatePostprint (published version

Cucumis metuliferus como potencial portainjerto de melón para el control de Meloidogine spp.

Se evaluó la eficacia de C. metuliferus sobre las densidades de población de M. incognita, la producción y calidad de melón, y la potencial selección de poblaciones virulentas. El melón cv. Paloma, sin injertar o injertado sobre C. metuliferus, se cultivó en primavera y verano en invernadero en rotación con tomate susceptible cv. Durinta, sin injertar o injertado en el portainjerto resistente Aligator, en suelo sin infestar e infestado con M. incognitaPostprint (published version

Arabidopsis_resurrection_13sep18

Flowering time data, microsatellite data and weather data from Iberian Arabidopsis thaliana accessions included in the resurrection experiments

Arabidopsis_data_RELICTS_8jul19

This file includes the following: (1) the predictors used in the species distribution model, (2) the paleobotanical data of three broad climatic periods (present, Holocene, Young Dryas - Allerod), and (3) the haplogroups of CRY2 and TSF genes obtained from publicly available whole-genome sequences

Review of the book The mystery of the Hanging Garden of Babylon. An elusive world wonder traced., Dalley, S.

Background: A collection of 175 melon (Cucumis melo L.) accessions (including wild relatives, feral types, landraces, breeding lines and commercial cultivars) from 50 countries was selected to study the phenotypic variability for ripening behavior and sugar accumulation. The variability of single nucleotide polymorphisms (SNPs) at 53 selected candidate genes involved in sugar accumulation and fruit ripening processes was studied, as well as their association with phenotypic variation of related traits.Results: The collection showed a strong genetic structure, defining seven groups plus a number of accessions that could not be associated to any of the groups (admixture), which fitted well with the botanical classification of melon varieties. The variability in candidate genes for ethylene, cell wall and sugar-related traits was high and similar to SNPs located in reference genes. Variability at ripening candidate genes had an important weight on the genetic stratification of melon germplasm, indicating that traditional farmers might have selected for ripening traits during cultivar diversification. A strong relationship was also found between the genetic structure and phenotypic diversity, which could hamper genetic association studies. Accessions belonging to the ameri group are the most appropriate for association analysis given the high phenotypic and molecular diversity within the group, and lack of genetic structure. The most remarkable association was found between sugar content and SNPs in LG III, where a hotspot of sugar content QTLs has previously been defined. By studying the differences in allelic variation of SNPs within horticultural groups with specific phenotypic features, we also detected differential variation in sugar-related candidates located in LGIX and LGX, and in ripening-related candidates located in LGII and X, all in regions with previously mapped QTLs for the corresponding traits.Conclusions: In the current study we have found an important variability at both the phenotypic and candidate gene levels for ripening behavior and sugar accumulation in melon fruit. By combination of differences in allelic diversity and association analysis, we have identified several candidate genes that may be involved in the melon phenotypic diversity.C.L. is recipient of a Marie Curie Career Integration Grant (CIG) and her visit to IBMCP-COMAV (Valencia) is supported by ESF Cost Action “FA1106 Quality Fruit” - Short-Term Scientific Mission (STSM). Genotyping was supported by SAFQIM project, AGL2012-40130-C02-02 from the Spanish Ministry of Economy and Competitivity (MINECO). Metabolite analysis at the MPIMP was supported by the German Federal Ministry of Education and Research (FP6 ERA-NET Plant Genomics project MELRIP; BMBF contract 0313987 to Mark Stitt)

Cucumis metuliferus es resistente a poblaciones de Meloidogyne spp. incluso virulentas al gen MI

Se realizaron diversos ensayos para determinar la respuesta de C. metuliferus frente diversas poblaciones de Meloidogyne arenaria, M. incognita y M. javanica, algunas de las cuales habían sido ya caracterizadas como virulentas al gen de resistencia Mien tomat

Pseudomonas aeruginosa antibiotic susceptibility profiles, genomic epidemiology and resistance mechanisms: a nation-wide five-year time lapse analysisResearch in context

Summary: Background: Pseudomonas aeruginosa healthcare-associated infections are one of the top antimicrobial resistance threats world-wide. In order to analyze the current trends, we performed a Spanish nation-wide high-resolution analysis of the susceptibility profiles, the genomic epidemiology and the resistome of P. aeruginosa over a five-year time lapse. Methods: A total of 3.180 nonduplicated P. aeruginosa clinical isolates from two Spanish nation-wide surveys performed in October 2017 and 2022 were analyzed. MICs of 13 antipseudomonals were determined by ISO-EUCAST. Multidrug resistance (MDR)/extensively drug resistance (XDR)/difficult to treat resistance (DTR)/pandrug resistance (PDR) profiles were defined following established criteria. All XDR/DTR isolates were subjected to whole genome sequencing (WGS). Findings: A decrease in resistance to all tested antibiotics, including older and newer antimicrobials, was observed in 2022 vs 2017. Likewise, a major reduction of XDR (15.2% vs 5.9%) and DTR (4.2 vs 2.1%) profiles was evidenced, and even more patent among ICU isolates [XDR (26.0% vs 6.0%) and DTR (8.9% vs 2.6%)] (p < 0.001). The prevalence of Extended-spectrum β-lactamase/carbapenemase production was slightly lower in 2022 (2.1%. vs 3.1%, p = 0.064). However, there was a significant increase in the proportion of carbapenemase production among carbapenem-resistant strains (29.4% vs 18.1%, p = 0.0246). While ST175 was still the most frequent clone among XDR, a slight reduction in its prevalence was noted (35.9% vs 45.5%, p = 0.106) as opposed to ST235 which increased significantly (24.3% vs 12.3%, p = 0.0062). Interpretation: While the generalized decrease in P. aeruginosa resistance, linked to a major reduction in the prevalence of XDR strains, is encouraging, the negative counterpart is the increase in the proportion of XDR strains producing carbapenemases, associated to the significant advance of the concerning world-wide disseminated hypervirulent high-risk clone ST235. Continued high-resolution surveillance, integrating phenotypic and genomic data, is necessary for understanding resistance trends and analyzing the impact of national plans on antimicrobial resistance. Funding: MSD and the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Unión Europea—NextGenerationEU