Innovative Collaboration in Agri-Food Systems

Tesis por compendio[ES] El objetivo general de esta Tesis ha sido avanzar en el estudio de los determinan-tes o características que pueden conducir a una colaboración exitosa entre los actores del sistema agroalimentario. Para ello, se analizaron las actividades de cooperación desarrolladas por explotaciones agrícolas, empresas agroalimentarias, instituciones y organizaciones vinculadas a la agroalimentación, que realizan ac-tividades innovadoras en España y América Latina y el Caribe (ALC). La investigación aborda diferentes ámbitos en los que se producen colaboraciones innovadoras. Se trata del ámbito científico, del ámbito de las plataformas multiac-tores y del ámbito de los agricultores y sus instituciones. Se ha estructurado en tres capítulos, correspondientes cada uno a un artículo científico publicado en una revista internacional. Cada uno de ellos aborda un aspecto específico para cumplir el objetivo general que se acaba de señalar. El primer artículo se titula "Collaboration for social innovation in the agri-food system in Latin America and the Caribbean". Este estudio tiene por objeto reali-zar una revisión del estado del conocimiento, utilizando técnicas bibliométricas, de la colaboración innovadora en los sistemas alimentarios y rurales de América Latina y el Caribe (ALC). Concretamente se analizan las actividades de coopera-ción desarrolladas por explotaciones agrícolas y empresas agroindustriales para emprender actividades innovadoras. El segundo artículo tiene como título "Collaboration through EIP-AGRI Opera-tional Groups and their role as innovation intermediaries". A través de una en-cuesta a los grupos operativos y mediante un análisis factorial fue posible identifi-car las funciones que estos grupos desarrollan como intermediarios de innovación desde su propia perspectiva. Este tipo de análisis permite abordar la colaboración entre actores de diferentes ámbitos y sectores para cooperar y aportar soluciones innovadoras a los problemas agrícolas. El tercer artículo, "Drivers of joint cropland management strategies in agri-food cooperatives", estudia la colaboración a través de una estrategia innovadora coor-dinada por las cooperativas. El objetivo principal es avanzar en el conocimiento de las características que presentan las cooperativas que lideran este tipo de iniciati-vas. El estudio se basa principalmente en los datos de una encuesta a cooperati-vas, y se ha utilizado una metodología de Análisis Cualitativo Comparativo (fsQCA). Los resultados confirman el avance en el estudio de la colaboración innovadora en el sector agroalimentario de ALC. Los principales motores de la colaboración en este ámbito son la innovación social, el conocimiento, la gestión sostenible y el capital social. También se observa un creciente interés en el ámbito científico por estudiar los sistemas de colaboración en ALC. Este interés proviene de científicos de diferentes países, lo que ha generado y potenciado la colaboración internacio-nal de los investigadores. Los resultados del segundo artículo se centran en las acciones de colaboración desarrolladas por plataformas multiactor, como lo son los Grupos Operativos. Las acciones desarrolladas por estos grupos pueden enmarcarse en las funciones de los intermediarios de la innovación. Tres funciones emergieron como las más realiza-das: la gestión del proceso de innovación, la articulación de la demanda, y el apo-yo institucional y la intermediación de la innovación. Por último, en el ámbito de los agricultores y sus instituciones, la investigación se centra en los impulsores del éxito de la colaboración. La innovación social y eco-nómica, el tamaño y la propensión a la cooperación son condiciones presentes en las cooperativas que tienen éxito al abordar iniciativas de gestión conjunta de cul[CA] L'objectiu general d'aquesta Tesi ha estat avançar en l'estudi dels determinants o característiques que poden conduir a una colaboración exitosa entre els actors del sistema agroalimentari. Per a això, s'analitzaren les activitats de cooperación desenvolupades per explotacions agrícolas, empreses agroalimentàries, institucions i organitzacions vinculades a la agroalimentació, que realitzen activitats innovadores a España i Amèrica Llatina i el Carib (ALC). La investigacicó aborda diferents àmbits en els que es produiexen collaboracions innovadores. Es tracta de l'àmbit científic, de l'àmbit de les plataformes multiactors i de l'àmbit dels agricultors i les seues institucions. S'ha estructurat en tres capítols, corresponents cadascun a un article científic publicat en una revista internacional. Cadascun d'ells aborda un aspecte específic per a acomplir l'objectiu general que s'acaba d'assenyalar. El primer artícle es titula "Collaboration for social innovation in the agri-food system in Latin America and the Caribbean". Aquest estudi té com a objectiu realitzar una revisió de l'estat del coneixement, utilitzant tècniques bibliomètriques, de la collaboració innovadora en els sistemes alimentaris i rurals d'Amèrica Llatina i el Carib (ALC). El segon article té com a títol "Collaboration through EIP-AGRI Operational Groups and their role as innovation intermediaries". A través d'una enquesta als grups operatius i mitjançant un anàlisi factorial va ser possible identificar les fun-cions que aquests grups desenvolupen com a intermediaris d'innovació des de la seua pròpia perspectiva. El tercer artícle, "Drivers of joint cropland management strategies in agri-food cooperatives", estudia la collaboració a través d'una estratègia innovadora coordinada per les cooperatives. L'objectiu principal és avançar en el coneixement de les característiques que presenten les cooperatives que lideren aquest tipus d'iniciatives. L'estudi es basa principalment en les dades d'una enquesta a cooperatives, i s'ha utilitzat una metodologia d'Anàlisi Qualitatiu Comparativo (fsQCA). Els resultats confirmen l'avançament en l'estudi de la col laboració innovadora en el sector agroalimentari de ALC. Els principals motors de col·laboració en aquest àmbit són la innovació social, el coneixement, la gestió sotenible i el capital social. També s'observa un creixent interès en l'àmbit científic per estudiar els sistemes de col·laboració en ALC. Aquest interès prové de científics de diferents països, el que ha generat i potenciat la col·laboració internacional dels investigadors. Els resultats del segon article es centren en les accions de col·laboració desenvolupades per plataformes multiactor, com ho són els Grups Operatius. Les accions desenvolupades per aquests grups poden enmarcarse en les funcions dels intermediaris de la innovació. Tres funcions emergeren com les més realitzades: la gestió del procés d'innovació, l'articulació de la demanda, i el suport institucional i la intermediació de la innovació. Per últim, en l'àmbit dels agricultors i les seues institucions, la investigació es centra en els impulsors de l'éxit de la col·laboració. La innovació social i econòmica, el tamany i la propensió a la cooperació són condicionants presents en les cooperatives que tenen èxit a l'abordar iniciatives de gestió conjunta de cultius. A partir dels resultats exposats, podem concloure que la colaboració per a la innovació social e en el sector rural pot ser una forma d'abordar els problemes estructurals en dife-rents àmbits. El coneixement dels principals determinants de col·laboració en el medi rural i agroalimentari a Espanya i ALC permetrà prendre millors decisions en les organitzacions públiques i privades per a promoure accions innovadores de cooperació en els territoris rurals. Futures investigacions poden basar-se en aquests resultats i avançar en altres característiques que determinen l'éxit de la col·laboració innovadora en el sector agroalimentari.[EN] The overall aim of this thesis is to identify the determinants of or characteristics that can lead to successful collaboration between actors in the agri-food system. To this end, an analysis is conducted of the cooperation activities developed by farms, agribusiness companies, institutions, and agri-food organisations, which undertake innovative actions in Spain and Latin America and the Caribbean (LAC). The research addresses different domains where innovative collaborations occur; namely, the scientific domain, the multi-actor platform domain, and the domain of farmers and their institutions. The resulting thesis is structured in three chapters, corresponding to three scientific articles published in international journals. Each of them deals with a specific aspect, which together fulfil the aforementioned research objective. The first article is entitled "Collaboration for social innovation in the agri-food system in Latin America and the Caribbean." Using bibliometric techniques, this study aims to review the state of the knowledge on innovative collaboration in LAC's food and rural systems. Specifically, it analyses the cooperation by farms and agribusiness firms to undertake innovative activities. The second article is entitled "Collaboration through EIP-AGRI Operational Groups and their role as innovation intermediaries." A survey to operational groups (OG) and a factor analysis are carried out to identify these groups' perspective on their roles as innovation intermediaries. This type of analysis makes it possible to address the collaboration between actors from different fields and sectors aimed at cooperating and providing innovative solutions to agricultural problems. The third article, "Drivers of joint cropland management strategies in agri-food cooperatives," studies collaboration through an innovative strategy coordinated by cooperatives. The main objective is to advance the knowledge of the characteristics of the cooperatives that head up this type of initiative. The study is mainly based on data from a survey of cooperative managers about their views on the main advantages and drivers of joint land management strategies, and the methodology applied is Fuzzy Set Qualitative Comparative Analysis (fsQCA). Findings confirm that progress has been made in the study of innovative collaboration in the LAC agri-food sector. The main drivers of collaboration include social innovation, knowledge, sustainable management, and social capital. In the scientific domain, growing academic interest in collaborative systems in LAC can be seen. Studies have been produced by researchers from different countries, which has generated and enhanced collaboration among international researchers. Results from the second article focus on the collaborative actions developed by multi-actor platforms. These actions can be framed as functions of innovation intermediaries. Three of the most common functions identified are innovation process management, demand articulation, and institutional support and innovation brokering. Finally, in the domain of farmers and their institutions, the research focuses on the drivers of successful collaboration. Social and economic innovation, size, and propensity for cooperation characterise the cooperatives that successfully take on a joint cropland initiative. From the results, we can conclude that collaboration for social innovation in the rural sector can be a way to address structural problems in different domains. Knowledge of the main determinants of collaboration in the rural and agri-food environment in Spain and LAC will help public and private organisations to make better decisions aimed at promoting innovative cooperation actions in rural territories. Future research can build on these results and focus on other characteristics that determine successful innovative collaboration in the agri-food sector.Gracias al Proyecto “Strengthening innovation policy in the agri-food sector” (RTI2018-093791-B-C22) financed by MCIN/AEI/10.13039/501100011033/ and “ERDF A way to build Europe”Piñeiro, VA. (2022). Innovative Collaboration in Agri-Food Systems [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/185684TESISCompendi

Magnetically propelled chained nanocomposites for biologically relevant media exploration

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGElongated nanostructures to be remotely and magnetically propelled in biologically relevant media, have gained attention as offering themselves as effective tools or carriers in theragnostics applications. However, the magnetic actuation associated remains challenging due to the lack of mechanical information in the media of interest, taking into account biophysical or biomedical purposes. In this study, we detail the magnetic actuation of magnetically propelled chained nanocomposites considering their dynamics, in which their velocity can be modulated in terms of the viscosity of the medium considered, given a magnetic field gradient. Simpler cases of distilled water, a water/glycerol mixture and a fluid made of cell extracts (imitating the cytosol of cells) of known viscosity are the basis experiments for the study of more complex media inside HeLa cells, murine NIH-3T3 fibroblasts and zebrafish larvae, offering the mechanical information required. The experimental results indicate that the magnetically propelled performance of the chained nanostructures can be precisely controlled in potentially changing scenarios, where drug and heat delivery, magnetic separation, or microfluidic technologies are demanded, using a magnetic field gradient and providing good estimations of the dynamical parameters involved.Xunta de Galicia | Ref. ED481A/322Xunta de Galicia | Ref. IN606A-2018/019Xunta de Galicia | Ref. IN853B 2018/03Ministerio de Economía y Competitividad | Ref. PI16/00496Instituto de Salud Carlos III | Ref. PI19/00349Instituto de Salud Carlos III | Ref. DTS19/00033Agencia Estatal de Investigación | Ref. MAT2016-81955-REDTAgencia Estatal de Investigación | Ref. PID2020-119242-I0

Calidad Territorial y Desarrollo Sustentable: Modelo de gestión para PyMes de agroturismo

El presente trabajo procura diseñar las bases de un Sistema de Gestión de Calidad con enfoque territorial, que en el marco del Desarrollo Rural Sustentable genere ventajas competitivas para la actividad agroturística de Pymes integrantes del Grupo Cambio Rural, Instituto Nacional de Tecnología Agropecuaria (INTA), “Cortaderas II” en el SO bonaerense. Se trata de un estudio de caso, para el cual se relevó información a través de entrevistas, encuestas y observación directa. El modelo se formula adaptando los elementos, principios y valores del sello “Marca de Calidad Territorial” europeo e incorpora un proceso de certificación participativa considerando la experiencia de Brasil, más cercana a la realidad argentina. Se concluye que los emprendimientos bajo estudio se encuentran en condiciones de aplicar dicha propuesta por los logros obtenidos en la dinámica de trabajo grupal, pudiendo así superar las dificultades de un proceso individual de certificación de normas de calidad, para avanzar en esta etapa cuando estén preparados.This paper attempts to design the basis for a Quality Management System with a territorial approach, which within the framework of Sustainable Rural Development generates competitive advantages for Agritourism SME’s belonging to the Rural Change Group, National Institute of Agricultural Technology, "Cortaderas II" in the south west of the Buenos Aires province. In this case study, information was gathered through interviews, surveys and direct observation. The model is formulated adapting the elements, principles and values of the European "Territorial Quality Mark" and it incorporates a participatory certification process considering the Brazilian experience, which is similar to what happens in Argentina. We conclude that, due to their successful group work dynamics, the enterprises under study are in a condition to implement this proposal and can thus overcome the difficulties of undergoing an individual certification process of quality standards in order to move on when they are prepared.Fil: Duran, Regina del Carmen. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Scoponi, Liliana. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Bustos Cara, Roberto Nicolas. Universidad Nacional del Sur. Departamento de Geografía y Turismo; ArgentinaFil: de Batista, Marianela. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Piñeiro, Verónica Ana. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Casarsa, Fabiana Andrea. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Cordisco, Marina. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Haag, Maria Isabel. Universidad Nacional del Sur. Departamento de Geografía y Turismo; ArgentinaFil: Brandauer, Guillermo. Universidad Nacional del Sur. Departamento de Ciencias de la Administración; ArgentinaFil: Presa, Cecilia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Colonnella, Julieta. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Chaz Sardi, María Celeste. Universidad Nacional del Sur. Departamento de Economía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentin

Cruise Summary Report - MEDWAVES survey. MEDiterranean out flow WAter and Vulnerable EcosystemS (MEDWAVES)

The MEDWAVES (MEDiterranean out flow WAter and Vulnerable EcosystemS) cruise targeted areas under the potential influence of the MOW within the Mediterranean and Atlantic realms. These include seamounts where Cold-water corals (CWCs) have been reported but that are still poorly known, and which may act as essential “stepping stones” connecting fauna of seamounts in the Mediterranean with those of the continental shelf of Portugal, the Azores and the Mid-Atlantic Ridge. During MEDWAVES sampling has been conducted in two of the case studies of ATLAS: Case study 7 (Gulf of Cádiz-Strait of Gibraltar-Alboran Sea) and Case study 8 (Azores). The initially targeted areas in the Atlantic were: the Gazul Mud volcano, in the Gulf of Cádiz (GoC) area, included in the case study 7, and the Atlantic seamounts Ormonde (Portuguese shelf) and Formigas (by Azores), both part of the case study 8. In the Mediterranean the targeted areas were The Guadiaro submarine canyon and the Seco de los Olivos (also known as Chella Bank) seamount. Unfortunately it was not possible to sample in Guadiaro due to time constraints originated by adverse meteorological conditions which obligate us to reduce the time at sea focusing only in 4 of the 5 initially planned areas. MEDWAVES was structured in two legs; the first leg took place from the 21st September (departure from Cádiz harbour in Spain) to the 13th October 2016 (arrival in Ponta Delgada, São Miguel, Azores, Portugal took place the 8th of October due to the meteorological conditions that obligated to conclude the first leg earlier as planned). during the Leg 1 sampling was carried out in Gazul, Ormonde and Formigas. The second leg started the 14th October (departure from Ponta Delgada) and finished the 26th October (arrival in Málaga harbour, Spain). MEDWAVES had a total of 30 effective sampling days, being 6 days not operative due to the adverse meteorological conditions experienced during the first leg which forced us to stay in Ponta Delgada from the 08th to the 13th October. During MEDWAVES the daily routine followed a similar scheme, depending of course on the weather and sea conditions. The main activity during the day, starting early in the morning (around 08:00 AM, once the night activities were finished), was the ROV deployment. Generally a single ROV dive of around 8 hours was performed, however in several occasions two dives were carried out in the same day (see General station list, Appendix II). After the ROV (and sometimes between two dives) the Box Corer and/or Van Veen Grab and/or Multicore was deployed. After these activities, during the night CTD-Rosette deployments and MB was conducted. Accordingly to this schema the scientific personnel worked in the day or in the night watch. A total of 215 sampling stations have been covered in MEDWAVES, using the following sampling gears: Multibeam echosounder, CTD-Rosette, LADCP, Box Corer, Van Veen Grab, Multicorer and a Remotely Operated Vehicle (ROV). Table 1 sumamrised the number of sampling stations conducted with each gear in each sampling zone. Additionally MB surveys have been conducted during the transits between area

The first wave of the COVID-19 epidemic in Spain was associated with early introductions and fast spread of a dominating genetic variant

SeqCOVID-Spain consortium: Álvaro Chiner-Oms, Irving Cancino-Muñoz, Mariana G. López, Manuela Torres-Puente, Inmaculada Gómez-Navarro, Santiago Jiménez-Serrano, Jordi Pérez-Tur, Darío García de Viedma, Laura Pérez-Lago, Marta Herranz, Jon Sicilia, Pilar Catalán-Alonso, Julia Suárez González, Patricia Muñoz, Mireia Coscolla, Paula Ruiz-Rodríguez, Fernando González-Candelas, Iñaki Comas, Lidia Ruiz-Roldán, María Alma Bracho, Neris García-González, Llúcia Martínez Priego, Inmaculada Galán-Vendrell, Paula Ruiz-Hueso, Griselda De Marco, María Loreto Ferrús-Abad, Sandra Carbó-Ramírez, Giuseppe D’Auria, Galo Adrian Goig, Juan Alberola, Jose Miguel Nogueira, Juan José Camarena, David Navarro, Eliseo Albert, Ignacio Torres, Maitane Aranzamendi Zaldumbide, Óscar Martínez Expósito, Nerea Antona Urieta, María de Toro, María Pilar Bea-Escudero, Jose Antonio Boga, Cristian Castelló-Abietar, Susana Rojo-Alba, Marta Elena Álvarez-Argüelles, Santiago Melón, Elisa Martró, Antoni E. Bordoy, Anna Not, Adrián Antuori, Anabel Fernández-Navarro, Andrés Canut-Blasco, Silvia Hernáez Crespo, Maria Luz Cordón Rodríguez, Maria Concepción Lecaroz Agara, Carmen Gómez-González, Amaia Aguirre-Quiñonero, José Israel López-Mirones, Marina Fernández-Torres, Maria Rosario Almela-Ferrer, Ana Carvajal, Juan Miguel Fregeneda-Grandes, Héctor Argüello, Gustavo Cilla Eguiluz, Milagrosa Montes Ros, Luis Piñeiro Vázquez, Ane Sorarrain, José María Marimón, José J. Costa-Alcalde, Rocío Trastoy, Gema Barbeito Castiñeiras, Amparo Coira, María Luisa Pérez del Molino, Antonio Aguilera, Begoña Palop-Borrás, Inmaculada de Toro Peinado, Maria Concepción Mediavilla Gradolph, Mercedes Pérez-Ruiz, Mirian Fernández-Alonso, Jose Luis del Pozo, Oscar González-Recio, Mónica Gutiérrez-Rivas, Jovita Fernández-Pinero, Miguel Ángel Jiménez Clavero, Begoña Fuster Escrivá, Concepción Gimeno Cardona, María Dolores Ocete Mochón, Rafael Medina-Gonzalez, José Antonio Lepe, Verónica González Galán, Ángel Rodríguez-Villodres, Nieves Gonzalo Jiménez, Jordi Reina, Carla López-Causapé, Maria Dolores Gómez-Ruiz, Eva M. Gonzalez-Barbera, José Luis López-Hontangas, Vicente Martín, Antonio J. Molina, Tania Fernandez-Villa, Ana Milagro Beamonte, Nieves Felisa Martínez-Cameo, Yolanda Gracia-Grataloup, Rosario Moreno-Muñoz, Maria Dolores Tirado Balaguer, José María Navarro-Marí, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gámez, Antonio Oliver, Mónica Parra Grande, Bárbara Gómez Alonso, Francisco José Arjona Zaragozí, Maria Carmen Pérez González, Francisco Javier Chamizo López, Ana Bordes-Benítez, Núria Rabella, Ferran Navarro, Elisenda Miró, Antonio Rezusta, Alexander Tristancho, Encarnación Simarro Córdoba, Julia Lozano-Serra, Lorena Robles Fonseca, Álex Soriano, Francisco Javier Roig Sena, Hermelinda Vanaclocha Luna, Isabel Sanmartín, Daniel García-Souto, Ana Pequeño-Valtierra, Jose M. C. Tubio, Javier Temes, Jorge Rodríguez-Castro, Martín Santamarina García, Manuel Rodríguez-Iglesias, Fátima Galán-Sanchez, Salud Rodríguez-Pallares, José Manuel Azcona-Gutiérrez, Miriam Blasco-Alberdi, Alfredo Mayor, Alberto L. García-Basteiro, Gemma Moncunill, Carlota Dobaño, Pau Cisteró, Oriol Mitjà, Camila González-Beiras, Martí Vall-Mayans, Marc Corbacho-Monné, Andrea Alemany, Cristina Muñoz-Cuevas, Guadalupe Rodríguez-Rodríguez, Rafael Benito, Sonia Algarate, Jessica Bueno, Andrea Vergara-Gómez, Miguel J. Martínez, Jordi Vila, Elisa Rubio, Aida Peiró-Mestres, Jessica Navero-Castillejos, David Posada, Diana Valverde, Nuria Estévez, Iria Fernández-Silva, Loretta de Chiara, Pilar Gallego-García, Nair Varela, Ulises Gómez-Pinedo, Mónica Gozalo-Margüello, Maria Eliecer Cano García, José Manuel Méndez-Legaza, Jesus Rodríguez-Lozano, María Siller, Daniel Pablo-Marcos, Maria Montserrat Ruiz-García, Antonio Galiana, Judith Sánchez-Almendro, Maria Isabel Gascón Ros, Cristina Juana Torregrosa-Hetland, Eva María Pastor Boix, Paloma Cascales Ramos, Pedro Luis Garcinuño Enríquez, Salvador Raga Borja, Julia González Cantó, Olalla Martínez Macias, Adolfo de Salazar, Laura Viñuela González, Natalia Chueca, Federico García, Cristina Gómez-Camarasa, Amparo Farga Martí, Rocío Falcón, Victoria Domínguez-Márquez, Anna M. Planas, Israel Fernández-Cádenas, Maria Ángeles Marcos, Carmen Ezpeleta, Ana Navascués, Ana Miqueleiz Zapatero, Manuel Segovia, Antonio Moreno-Docón, Esther Viedma, Raúl Recio Martínez, Irene Muñoz-Gallego, Sara Gonzalez-Bodi, Maria Dolores Folgueira, Jesús Mingorance, Elias Dahdouh, Fernando Lázaro-Perona, María Rodríguez-Tejedor, María Pilar Romero-Gómez, Julio García-Rodríguez, Juan Carlos Galán, Mario Rodríguez-Dominguez, Laura Martínez-García, Melanie Abreu Di Berardino, Manuel Ponce-Alonso, Jose Maria González-Alba, Ivan Sanz-Muñoz, Diana Pérez San José, Maria Gil Fortuño, Juan B. Bellido-Blasco, Alberto Yagüe Muñoz, Noelia Hernández Pérez, Helena Buj Jordá, Óscar Pérez Olaso, Alejandro González Praetorius, Nora Mariela Martínez Ramírez, Aida Ramírez Marinero, Eduardo Padilla León, Alba Vilas Basil, Mireia Canal Aranda, Albert Bernet Sánchez, Alba Bellés Bellés, Eric López González, Iván Prats Sánchez, Mercè García-González, Miguel José Martínez-Lirola, Manuel Ángel Rodríguez Maresca, Maria Teresa Cabezas Fernández, María Eugenia Carrillo Gil, Maria Paz Ventero Martín, Carmen Molina Pardines, Nieves Orta Mira, María Navarro Cots, Inmaculada Vidal Catalá, Isabel García Nava, Soledad Illescas Fernández-Bermejo, José Martínez-Alarcón, Marta Torres-Narbona, Cristina Colmenarejo, Lidia García-Agudo, Jorge A. Pérez García, Martín Yago López, María Ángeles Goberna Bravo, Victoria Simón García, Gonzalo Llop Furquet, Agustín Iranzo Tatay, Sandra Moreno-Marro, Noelia Lozano Rodríguez, Amparo Broseta Tamarit, Juan José Badiola Díez, Amparo Martínez-Ramírez, Ana Dopazo, Sergio Callejas, Alberto Benguría, Begoña Aguado, Antonio Alcamí, Marta Bermejo Bermejo, Ricardo Ramos-Ruíz, Víctor Manuel Fernández Soria, Fernando Simón Soria & Mercedes Roig CardellsThe coronavirus disease 2019 (COVID-19) pandemic has affected the world radically since 2020. Spain was one of the European countries with the highest incidence during the first wave. As a part of a consortium to monitor and study the evolution of the epidemic, we sequenced 2,170 samples, diagnosed mostly before lockdown measures. Here, we identified at least 500 introductions from multiple international sources and documented the early rise of two dominant Spanish epidemic clades (SECs), probably amplified by superspreading events. Both SECs were related closely to the initial Asian variants of SARS-CoV-2 and spread widely across Spain. We inferred a substantial reduction in the effective reproductive number of both SECs due to public-health interventions (Re < 1), also reflected in the replacement of SECs by a new variant over the summer of 2020. In summary, we reveal a notable difference in the initial genetic makeup of SARS-CoV-2 in Spain compared with other European countries and show evidence to support the effectiveness of lockdown measures in controlling virus spread, even for the most successful genetic variants.This work was mainly funded by the Instituto de Salud Carlos III project COV20/00140, with additional funding by Spanish National Research Council project CSIC-COV19-021, Ministerio de Ciencia project PID2019-104477RB-100, ERC StG 638553 and ERC CoG 101001038 to I.C., and BFU2017-89594R to F.G.C. M.C. is supported by Ramón y Cajal program from Ministerio de Ciencia and grants RTI2018-094399-A-I00 and Generalitat Valenciana (Regional Government) project SEJI/2019/011. We gratefully acknowledge Hospital Universitari Vall d’Hebron, Instituto de Salud Carlos III, IrsiCaixa AIDS Research Lab and all the international researchers and institutions that submitted sequenced SARS-CoV-2 genomes to the GISAID’s EpiCov Database (Supplementary Table 1), as an important part of our analyses has been made possible by the sharing of their work. We also thank Unidad de Bioinformática y Estadística, Centro de Investigación Príncipe Felipe, for allowing us to use the Computer Cluster to perform some of the bioinformatic analysis.Peer reviewe

Magnetically propelled chained nanocomposites for biologically relevant media exploration

Elongated nanostructures to be remotely and magnetically propelled in biologically relevant media, have gained attention as offering themselves as effective tools or carriers in theragnostics applications. However, the magnetic actuation associated remains challenging due to the lack of mechanical information in the media of interest, taking into account biophysical or biomedical purposes. In this study, we detail the magnetic actuation of magnetically propelled chained nanocomposites considering their dynamics, in which their velocity can be modulated in terms of the viscosity of the medium considered, given a magnetic field gradient. Simpler cases of distilled water, a water/glycerol mixture and a fluid made of cell extracts (imitating the cytosol of cells) of known viscosity are the basis experiments for the study of more complex media inside HeLa cells, murine NIH-3T3 fibroblasts and zebrafish larvae, offering the mechanical information required. The experimental results indicate that the magnetically propelled performance of the chained nanostructures can be precisely controlled in potentially changing scenarios, where drug and heat delivery, magnetic separation, or microfluidic technologies are demanded, using a magnetic field gradient and providing good estimations of the dynamical parameters involved.Acknowledgments: M. A. R.-D. acknowledges financial support from the Xunta de Galicia (Regional Government, Spain) under grant 2017-ED481A/322. P. H. is a recipient of a Predoctoral fellowship (IN606A-2018/019) from Axencia Galega de Innovación (GAIN, Xunta de Galicia). R. P. and A. B. D.-I. are supported by Roche-Chus Joint Unit (IN853B 2018/03) funded by GAIN, Consellería de Economía, Emprego e Industria, Xunta de Galicia. A. B. D.-I. acknowledges financial support from the Ministerio de Economía y Competitividad under Sara Borrell contract. M. L. F. acknowledge the financial support from the Spanish MINECO, Instituto de Salud Carlos III and the European Union FEDER funds under Projects ref. PI16/00496, PI19/00349, DTS19/00033 and the NanoBioApp Network (MINECO-17-MAT2016-81955-REDT). V. S. acknowledges the financial support from the Spanish Ministerio de Ciencia e Innovación under project PID2020-119242-I00 and from the European Union under project H2020-MSCA-RISE-2019 PEPSA-MATE (project number 872233). V. S. acknowledges for funding for open access charge: Universidade de Vigo/CISUG.Magnetic swimmersMagnetophoretic mobilityViscosityZebrafish yolk sa

Methylphenidate Promotes Premature Growth Plate Closure: In Vitro Evidence

It is well known that patients with attention deficit hyperactivity disorder treated with stimulants, such as methylphenidate hydrochloride (MPH), have reduced height and weight. Even though MPH has an anorexigenic effect, an additional impact of this drug on the growth plate cannot be discarded. In this study, we aimed to determine the cellular effect of MPH on an in vitro growth plate model. We tested the effects of MPH on the viability and proliferation of a prechondrogenic cell line via an MTT assay. In vitro differentiation of this cell line was performed, and cell differentiation was evaluated through the expression of cartilage- and bone-related genes as measured via RT-PCR. MPH did not alter the viability or proliferation of prechondrogenic cells. However, it reduced the expression of cartilage extracellular matrix-related genes (type II collagen and aggrecan) and increased the expression of genes involved in growth plate calcification (Runx2, type I collagen, and osteocalcin) at different phases of their differentiation process. Our results evidence that MPH upregulates genes associated with growth plate hypertrophic differentiation. This may induce premature closure of the growth plate, which would contribute to the growth retardation that has been described to be induced by this drug

Effect and Regulation of Obesity-Associated Low-Grade Chronic Inflammation in Major Rheumatic Diseases

Current lifestyle and environmental factors contribute to obesity development, leading to low-grade chronic inflammation (LGCI). Apart from obesity, LGCI is also related to rheumatic diseases such as osteoporosis (OP) and osteoarthritis (OA). In these, an excessive accumulation of adipose tissue has been linked to an excessive production of proinflammatory factors, such as adipokines. This work’s aim is to stablish the effect of obesity-associated LGCI in major rheumatic diseases and to determine optimal strategies to reduce it. Obesity is a risk factor for developing OA, where a systemic LGCI state has been found. Concretely, obesity-associated LGCI has been described as an OA instauration and progression promoter. To avoid this, several therapeutical approaches (diet control, physical exercise, or nutraceuticals) have been tested. OP is another major rheumatic disease where a basal LGCI has been described, being worsened by obesity. As in OA, diet management and supplementation with vitamin D or probiotics have been proposed as approaches to treat obesity-associated LGCI in this pathology. Currently, the increase in the prevalence of rheumatic diseases is unstoppable. Nonetheless, obesity is a risk factor that can be controlled. Thus, the study of new interventions to control the impact of obesity-associated LGCI is a challenge for the management of patients with rheumatic diseases

The hidden diversity: invertebrates of temporary ponds in Barra Grande Uruguay.

Los charcos temporales de Uruguay y los invertebrados que en estos habitan representan ecosistemas relativamente poco explorados,sin referencias claras en relación a los niveles de diversidad que presentan. En el presente trabajo reportamos la presencia de 216 especies y morfoespecies de invertebrados y una estimación total de riqueza entre 263 y 373 morfoespecies, para 18 charcos temporales de la cuenca de la Laguna Castillos, en el departamento de Rocha, Uruguay. Esta diversidad permitiría mantener la estabilidad del funcionamiento del ecosistema y los servicios que este brinda como ser agua potable, forraje para el ganado, limitación en la abundancia de patógenos y brotes de enfermedades o resistencia del sistema frente a contaminantes. Los estudios ecológicos en charcos temporalesconstituyenexcelentesmodelosparaestudios de biodiversidad ya que proveen resultados generalizables a otros ecosistemas. Sin embargo, una de las limitaciones que encontramos en este trabajo fue la dificultad para la identificación taxonómica de los invertebrados. La gestión y manejo de la diversidad necesita una base taxonómica sólida que permita conocer la composición y estructura de dicha diversidad. Por lo tanto, resaltamos la importancia de realizar estudios en sistemática de invertebrados de agua dulce en Uruguay.Temporary ponds of Uruguay and the invertebrates that inhabit them represent relatively unexplored ecosystems,without clear references in relation to the diversity levels they host. In the present work we report the presence of 216 species and morphospecies of invertebrates and a total estimate of richness between 263 and 373 species, for 18 temporary ponds of Castillos Lagoon Basin, in the department of Rocha, Uruguay. This high species diversity would allow maintaining the stability of ecosystem functioning and the services it provides, such as drinking water, attenuation of hydrological pulses in lagoons and rivers, forage for livestock, limitation in the abundance of pathogens and disease outbreaks or system resistance against contaminants. Ecological studies in temporary ponds are excellent models for biodiversity studies and provide results that can be generalized too ther ecosystems. However, one of the limitations raised in this work was the difficulty for the taxonomic identification of invertebrates. The management of diversity needs a solid taxonomic base that allows knowing the composition and structure of that diversity. Therefore, we highlight the importance of systematic studies of fresh water invertebrates in Uruguay.CSIC: Grupos-657725

Synergism interaction between genetic polymorphisms in drug metabolizing enzymes and NSAIDs on upper gastrointestinal haemorrhage : a multicenter case-control study

Interindividual genetic variations contribute to differences in patients' response to drugs as well as to the development of certain disorders. Patients who use non-steroidal anti-inflammatory drugs (NSAIDs) may develop serious gastrointestinal disorders, mainly upper gastrointestinal haemorrhage (UGIH). Studies about the interaction between NSAIDs and genetic variations on the risk of UGIH are scarce. Therefore, we investigated the effect of 16 single nucleotide polymorphisms (SNPs) involved in drug metabolism on the risk of NSAIDs-induced UGIH. We conducted a multicenter case-control study of 326 cases and 748 controls. Participants were sub-grouped into four categories according to NSAID exposure and genetic profile. We estimated odds ratios (ORs) and their 95% confidence intervals (CI) using generalized linear mixed models for dependent binomial variables and then calculated the measures of interaction, synergism index (S), and relative excess risk due to interaction (RERI). We undertook stratified analyses by the type of NSAID (aspirin, non-aspirin). We observed an excess risk of UGIH due to an interaction between any NSAID, non-aspirin NSAIDs or aspirin and carrying certain SNPs. The greatest excess risk was observed for carriers of: rs2180314:C>G [any NSAID: S = 3.30 (95%CI: 1.24-8.80), RERI = 4.39 (95%CI: 0.70-8.07); non-aspirin NSAIDs: S = 3.42 (95%CI: 1.12-10.47), RERI = 3.97 (95%CI: 0.44-7.50)], and rs4809957:A>G [any NSAID: S = 2.11 (95%CI: 0.90-4.97), RERI = 3.46 (95%CI: −0.40-7.31)]. Aspirin use by carriers of rs6664:C>T is also associated with increased risk of UGIH [OR: 2.22 (95%CI: 0.69-7.17) vs. OR: 7.72 (95%CI: 2.75-21.68)], yet larger sample size is needed to confirm this observation. The joint effect of the SNPs s2180314:C>G and rs4809957:A>G and NSAIDs are more than three times higher than the sum of their individual effects. Personalized prescriptions based on genotyping would permit a better weighing of risks and benefits from NSAID consumptio