Piecewise constant potentials and discrete ambiguities

This work is devoted to the study of discrete ambiguities. For parametrized potentials, they arise when the parameters are fitted to a finite number of phase-shifts. It generates phase equivalent potentials. Such equivalence was suggested to be due to the modulo $\pi$ uncertainty inherent to phase determinations. We show that a different class of phase-equivalent potentials exists. To this aim, use is made of piecewise constant potentials, the intervals of which are defined by the zeros of their regular solutions of the Schr\"odinger equation. We give a classification of the ambiguities in terms of indices which include the difference between exact phase modulo $\pi$ and the numbering of the wave function zeros.Comment: 26 pages Subject: Mathematical Physics math-p

Habitation

Dans un contexte concurrentiel accru, les industries doivent développer de nouvelles solutions pour répondre à des défis majeurs (innovation, design, fonctionnalité, consommation…). La plastronique, qui est au confluent des domaines de la plasturgie et de l’électronique, permet de répondre à ces nouvelles attentes, en proposant des objets intelligents et connectés. Elle offre la possibilité de concevoir des systèmes de forme complexe 3D. Dans ce contexte de rupture technologique, l’innovation est à l’interface du génie électrique, de la mécatronique et de la plasturgie. Elle fait également appel à des domaines connexes et indispensables tels que la physique et la chimie. La convergence de ces domaines rend indispensable l’acquisition de nouvelles connaissances et compétences transverses dans les métiers de demain. Pour répondre à ces besoins, une formation en plastronique est en émergence à l’Université de Lyon. Les modalités seront décrites dans cet article. Les compétences initiales des apprenants seront élargies pour acquérir les bases théoriques et pratiques de la conception de systèmes plastroniques. Cette formation s’appuiera sur des projets concrets en lien direct avec les acteurs variés de la filière (aéronautique, médical, robotique, etc.). Le prototypage y tiendra une place importante, en permettant de confronter les apprenants à des problématiques industrielles concrètes

Interrupting peptidoglycan deacetylation during Bdellovibrio predator-prey interaction prevents ultimate destruction of prey wall, liberating bacterial-ghosts

The peptidoglycan wall, located in the periplasm between the inner and outer membranes of the cell envelope in Gram-negative bacteria, maintains cell shape and endows osmotic robustness. Predatory Bdellovibrio bacteria invade the periplasm of other bacterial prey cells, usually crossing the peptidoglycan layer, forming transient structures called bdelloplasts within which the predators replicate. Prey peptidoglycan remains intact for several hours, but is modified and then degraded by predators escaping. Here we show predation is altered by deleting two Bdellovibrio N-acetylglucosamine (GlcNAc) deacetylases, one of which we show to have a unique two domain structure with a novel regulatory-”plug”. Deleting the deacetylases limits peptidoglycan degradation and rounded prey cell “ghosts” persist after mutant-predator exit. Mutant predators can replicate unusually in the periplasmic region between the peptidoglycan wall and the outer membrane rather than between wall and inner-membrane, yet still obtain nutrients from the prey cytoplasm. Deleting two further genes encoding DacB/PBP4 family proteins, known to decrosslink and round prey peptidoglycan, results in a quadruple mutant Bdellovibrio which leaves prey-shaped ghosts upon predation. The resultant bacterial ghosts contain cytoplasmic membrane within bacteria-shaped peptidoglycan surrounded by outer membrane material which could have promise as “bacterial skeletons” for housing artificial chromosomes

Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates

Lytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinus similis (LsAA9A) and Collariella virescens (CvAA9A). LsAA9A and CvAA9A cleave a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan. LsAA9A additionally cleaves isolated xylan substrates. The structures of CvAA9A and of LsAA9A bound to cellulosic and non-cellulosic oligosaccharides provide insight into the molecular determinants of their specificity. Spectroscopic measurements reveal differences in copper co-ordination upon the binding of xylan and glucans. LsAA9A activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage. Overall, these data show that AA9 LPMOs can display different apparent substrate specificities dependent upon both productive protein–carbohydrate interactions across a binding surface and also electronic considerations at the copper active site

Endothelial Dysfunction and Specific Inflammation in Obesity Hypoventilation Syndrome

BACKGROUND: Obesity hypoventilation syndrome (OHS) is associated with increased cardiovascular morbidity. What moderate chronic hypoventilation adds to obesity on systemic inflammation and endothelial dysfunction remains unknown. QUESTION: To compare inflammatory status and endothelial function in OHS versus eucapnic obese patients. METHODOLOGY: 14 OHS and 39 eucapnic obese patients matched for BMI and age were compared. Diurnal blood gazes, overnight polysomnography and endothelial function, measured by reactive hyperemia peripheral arterial tonometry (RH-PAT), were assessed. Inflammatory (Leptin, RANTES, MCP-1, IL-6, IL-8, TNFalpha, Resistin) and anti-inflammatory (adiponectin, IL-1Ra) cytokines were measured by multiplex beads immunoassays. PRINCIPAL FINDINGS: OHS exhibited a higher PaCO(2), a lower forced vital capacity (FVC) and tended to have a lower PaO(2) than eucapnic obese patients. (HS)-CRP, RANTES levels and glycated haemoglobin (HbA1c) were significantly increased in OHS (respectively 11.1+/-10.9 vs. 5.7+/-5.5 mg x l(-1) for (HS)-CRP, 55.9+/-55.3 vs 23.3+/-15.8 ng/ml for RANTES and 7.3+/-4.3 vs 6.1+/-1.7 for HbA1c). Serum adiponectin was reduced in OHS (7606+/-2977 vs 13,660+/-7854 ng/ml). Endothelial function was significantly more impaired in OHS (RH-PAT index: 0.22+/-0.06 vs 0.51+/-0.11). CONCLUSIONS: Compared to eucapnic obesity, OHS is associated with a specific increase in the pro-atherosclerotic RANTES chemokine, a decrease in the anti-inflammatory adipokine adiponectin and impaired endothelial function. These three conditions are known to be strongly associated with an increased cardiovascular risk. TRIAL REGISTRATION: ClinicalTrials.gov NCT00603096

Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola Reveals Dispensome Structure, Chromosome Plasticity, and Stealth Pathogenesis.

The plant-pathogenic fungus Mycosphaerella graminicola (asexual stage: Septoria tritici) causes septoria tritici blotch, a disease that greatly reduces the yield and quality of wheat. This disease is economically important in most wheat-growing areas worldwide and threatens global food production. Control of the disease has been hampered by a limited understanding of the genetic and biochemical bases of pathogenicity, including mechanisms of infection and of resistance in the host. Unlike most other plant pathogens, M. graminicola has a long latent period during which it evades host defenses. Although this type of stealth pathogenicity occurs commonly in Mycosphaerella and other Dothideomycetes, the largest class of plant-pathogenic fungi, its genetic basis is not known. To address this problem, the genome of M. graminicolawas sequenced completely. The finished genome contains 21 chromosomes, eight of which could be lost with no visible effect on the fungus and thus are dispensable. This eight-chromosome dispensome is dynamic in field and progeny isolates, is different from the core genome in gene and repeat content, and appears to have originated by ancient horizontal transfer from an unknown donor. Synteny plots of the M. graminicola chromosomes versus those of the only other sequenced Dothideomycete, Stagonospora nodorum, revealed conservation of gene content but not order or orientation, suggesting a high rate of intra-chromosomal rearrangement in one or both species. This observed “mesosynteny” is very different from synteny seen between other organisms. A surprising feature of the M. graminicolagenome compared to other sequenced plant pathogens was that it contained very few genes for enzymes that break down plant cell walls, which was more similar to endophytes than to pathogens. The stealth pathogenesis of M. graminicola probably involves degradation of proteins rather than carbohydrates to evade host defenses during the biotrophic stage of infection and may have evolved from endophytic ancestors

Comparative genomics of the major parasitic worms

Parasitic nematodes (roundworms) and platyhelminths (flatworms) cause debilitating chronic infections of humans and animals, decimate crop production and are a major impediment to socioeconomic development. Here we report a broad comparative study of 81 genomes of parasitic and non-parasitic worms. We have identified gene family births and hundreds of expanded gene families at key nodes in the phylogeny that are relevant to parasitism. Examples include gene families that modulate host immune responses, enable parasite migration though host tissues or allow the parasite to feed. We reveal extensive lineage-specific differences in core metabolism and protein families historically targeted for drug development. From an in silico screen, we have identified and prioritized new potential drug targets and compounds for testing. This comparative genomics resource provides a much-needed boost for the research community to understand and combat parasitic worms

Formation au prototypage de systèmes électroniques 2D et 3D. Initiation à la Plastronique 3D et aux dispositifs MID

Nous présentons dans ce papier un projet de formation innovant relatif au prototypage de circuits et systèmes électronique 2D et 3D. Les apprenants seront initiés aux technologies de ruptures que sont la plastronique et les circuits imprimés 3D sur support rigide (Molded Interconnect Device ou Dispositifs Interconnectés Moulés). Ces dernières nécessitent d’acquérir des compétences aussi bien dans le domaine de la plasturgie que dans celui de l’électronique. Alors que la technologie et l’innovation se situent aux interfaces de ces domaines, il est aujourd’hui impératif de casser les modes de conceptions (usuellement en silo) et de repenser la fiche de route (roadmap). Seule une avancée conjointe permettra d’apporter et d’engranger un savoir-faire innovant pour converger vers des solutions pertinentes et une implémentation optimale. Nous souhaitons aller vers la création d’une plateforme dédiée. Cette dernière mutualisera des moyens techniques et humains et affichera une bonne visibilité. Le tissu industriel de la région Rhône-Alpes issue d’une longue histoire est à ce titre, particulièrement propice à l’émergence de cette action. Aujourd’hui, un groupe de travail associe des acteurs industriels et académiques avec pour objectif de mettre en place cette formation multi-physiques et multi-domaines alliant électronique, plasturgie, mécatronique

Caractérisation de capacités MIM Cu-Si3N4-Cu en technologie CMOS 0,12 µm pour des applications radiofréquences.

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