Variable masses in fission and heavy-ion collisions

With the use of the cranking formula, the coordinate-dependent mass parameters of the kinetic-energy operator in fission processes and heavy-ion collisions are calculated in the two-center oscillator model. It is shown that the reduced mass and also the classical moment of inertia are obtained for large separations of the fragments. For small separations, however, the mass parameter for the motion of the centers of mass of the fragments is larger than the reduced mass by an order of magnitude

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

Impact of Record-Linkage Errors in Covid-19 Vaccine-Safety Analyses using German Health-Care Data: A Simulation Study

With unprecedented speed, 192,248,678 doses of Covid-19 vaccines were administered in Germany by July 11, 2023 to combat the pandemic. Limitations of clinical trials imply that the safety profile of these vaccines is not fully known before marketing. However, routine health-care data can help address these issues. Despite the high proportion of insured people, the analysis of vaccination-related data is challenging in Germany. Generally, the Covid-19 vaccination status and other health-care data are stored in separate databases, without persistent and database-independent person identifiers. Error-prone record-linkage techniques must be used to merge these databases. Our aim was to quantify the impact of record-linkage errors on the power and bias of different analysis methods designed to assess Covid-19 vaccine safety when using German health-care data with a Monte-Carlo simulation study. We used a discrete-time simulation and empirical data to generate realistic data with varying amounts of record-linkage errors. Afterwards, we analysed this data using a Cox model and the self-controlled case series (SCCS) method. Realistic proportions of random linkage errors only had little effect on the power of either method. The SCCS method produced unbiased results even with a high percentage of linkage errors, while the Cox model underestimated the true effect

The Challenges of Wastewater Irrigation in Developing Countries

The volume of wastewater generated by domestic, industrial, and commercial sources has increased with population, urbanization, improved living conditions, and economic development. The productive use of wastewater has also increased, as millions of small-scale farmers in urban and peri-urban areas of developing countries depend on wastewater or wastewater-polluted water sources to irrigate high-value edible crops for urban markets, often as they have no alternative sources of irrigation water. Undesirable constituents in wastewater can harm human health and the environment. Hence, wastewater irrigation is an issue of concern to public agencies responsible for maintaining public health and environmental quality. For diverse reasons, many developing countries are still unable to implement comprehensive wastewater treatment programs. Therefore in the near term, risk management and interim solutions are needed to prevent adverse impacts from wastewater irrigation. A combination of source control and farm-level and post-harvest measures can be used to protect farm workers and consumers. The WHO guidelines revised in 2006 for wastewater use suggest measures beyond the traditional recommendations of producing only industrial or nonedible crops, as in many situations it is impossible to enforce a change in the current cash crop pattern, or provide alternative vegetable supply to urban markets. There are several opportunities for improving wastewater management via improved policies, institutional dialogues, and financial mechanisms, which would reduce the risks in agriculture. Effluent standards combined with incentives or enforcement can motivate improvements in water management by household and industrial sectors discharging wastewater from point sources. Segregation of chemical pollutants from urban waste-water facilitates treatment and reduces risk. Strengthening institutional capacity and establishing links between water delivery and sanitation sectors through interinstitutional coordination leads to more efficient management of wastewater and risk reduction

Analysis of a microscopic stochastic model of microtubule dynamic instability

A novel theoretical model of dynamic instability of a system of linear (1D) microtubules (MTs) in a bounded domain is introduced for studying the role of a cell edge in vivo and analyzing the effect of competition for a limited amount of tubulin. The model differs from earlier models in that the evolution of MTs is based on the rates of single unit (e.g., a heterodimer per protofilament) transformations, in contrast to postulating effective rates/frequencies of larger-scale changes, extracted, e.g., from the length history plots of MTs. Spontaneous GTP hydrolysis with finite rate after polymerization is assumed, and theoretical estimates of an effective catastrophe frequency as well as other parameters characterizing MT length distributions and cap size are derived. We implement a simple cap model which does not include vectorial hydrolysis. We demonstrate that our theoretical predictions, such as steady state concentration of free tubulin, and parameters of MT length distributions, are in agreement with the numerical simulations. The present model establishes a quantitative link between microscopic parameters governing the dynamics of MTs and macroscopic characteristics of MTs in a closed system. Lastly, we use a computational Monte Carlo model to provide an explanation for non-exponential MT length distributions observed in experiments. In particular, we show that appearance of such non-exponential distributions in the experiments can occur because the true steady state has not been reached, and/or due to the presence of a cell edge.Comment: 14 pages, 7 figure

Meningothelial Cells React to Elevated Pressure and Oxidative Stress

BACKGROUND: Meningothelial cells (MECs) are the cellular components of the meninges enveloping the brain. Although MECs are not fully understood, several functions of these cells have been described. The presence of desmosomes and tight junctions between MECs hints towards a barrier function protecting the brain. In addition, MECs perform endocytosis and, by the secretion of cytokines, are involved in immunological processes in the brain. However, little is known about the influence of pathological conditions on MEC function; e.g., during diseases associated with elevated intracranial pressure, hypoxia or increased oxidative stress. METHODS: We studied the effect of elevated pressure, hypoxia, and oxidative stress on immortalized human as well as primary porcine MECs. We used MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) bioreduction assays to assess the proliferation of MECs in response to treatment and compared to untreated control cells. To assess endocytotic activity, the uptake of fluorescently labeled latex beads was analyzed by fluorescence microscopy. RESULTS: We found that exposure of MECs to elevated pressure caused significant cellular proliferation and a dramatic decrease in endocytotic activity. In addition, mild oxidative stress severely inhibited endocytosis. CONCLUSION: Elevated pressure and oxidative stress impact MEC physiology and might therefore influence the microenvironment of the subarachnoid space and thus the cerebrospinal fluid within this compartment with potential negative impact on neuronal function

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts.publishedVersio

Protein homeostasis and aging in neurodegeneration

Genetic and environmental factors responsible for numerous neurodegenerative diseases vary between disorders, yet age remains a universal risk factor. Age-associated decline in protein homeostasis, or proteostasis, enables disease-linked proteins to adopt aberrant tertiary structures, accumulate as higher-ordered aggregates, and cause a myriad of cellular dysfunctions and neuronal death. However, recent findings suggest that the assembly of disease proteins into tightly ordered aggregates can significantly delay proteotoxic onset. Furthermore, manipulation of metabolic pathways through key signaling components extends lifespan, bolsters proteostasis networks, and delays the onset of proteotoxicity. Thus, understanding the relationship between proteostasis and aging has provided important insights into neurodegeneration