Free Differential Algebras: Their Use in Field Theory and Dual Formulation

The gauging of free differential algebras (FDA's) produces gauge field theories containing antisymmetric tensors. The FDA's extend the Cartan-Maurer equations of ordinary Lie algebras by incorporating p-form potentials ($p > 1$). We study here the algebra of FDA transformations. To every p-form in the FDA we associate an extended Lie derivative $\ell$ generating a corresponding ``gauge" transformation. The field theory based on the FDA is invariant under these new transformations. This gives geometrical meaning to the antisymmetric tensors. The algebra of Lie derivatives is shown to close and provides the dual formulation of FDA's.Comment: 10 pages, latex, no figures. Talk presented at the 4-th Colloquium on "Quantum Groups and Integrable Sysytems", Prague, June 199

Probing neutrino masses with CMB lensing extraction

We evaluate the ability of future cosmic microwave background (CMB) experiments to measure the power spectrum of large scale structure using quadratic estimators of the weak lensing deflection field. We calculate the sensitivity of upcoming CMB experiments such as BICEP, QUaD, BRAIN, ClOVER and PLANCK to the non-zero total neutrino mass M_nu indicated by current neutrino oscillation data. We find that these experiments greatly benefit from lensing extraction techniques, improving their one-sigma sensitivity to M_nu by a factor of order four. The combination of data from PLANCK and the SAMPAN mini-satellite project would lead to sigma(M_nu) = 0.1 eV, while a value as small as sigma(M_nu) = 0.035 eV is within the reach of a space mission based on bolometers with a passively cooled 3-4 m aperture telescope, representative of the most ambitious projects currently under investigation. We show that our results are robust not only considering possible difficulties in subtracting astrophysical foregrounds from the primary CMB signal but also when the minimal cosmological model (Lambda Mixed Dark Matter) is generalized in order to include a possible scalar tilt running, a constant equation of state parameter for the dark energy and/or extra relativistic degrees of freedom.Comment: 13 pages, 4 figures. One new figure and references added. Version accepted for publicatio

Zinc ions alter morphology and chitin deposition in an ericoid fungus

A sterile mycelium PS IV, an ascomycete capable of establishing ericoid mycorrhizas, was used to investigate how zinc ions affect the cellular mechanisms of fungal growth. Asignificant reduction of the fungal biomass was observed in the presence of millimolar zinc concentrations; this mirrored conspicuous changes in hyphal morphology which led to apical swellings and increased branching in the subapical parts. Specific probes for fluorescence and electron microscopy localised chitin, the main cell wall polysaccharide, on the inner part of the fungal wall and on septa in control specimens. In Zn-treated mycelium, hyphal walls were thicker and a more intense chitin labelling was detected on the transverse walls. Aquantitative assay showed a significant increase in the amount of chitin in metal- treated hyphae

Electrospun silk fibroin fibers for storage and controlled release of human platelet lysate

Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration of different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption has been limited because of its rapid degradation at room temperature that decreases its functionality. Another limiting factor for its extensive use is the difficulty of handling the hPL gels. In this work, silk fibroin-based patches were developed to address several points: improving the handling of hPL, enabling their delivery in a controlled manner and facilitating their storage by creating a device ready to use with expanded shelf life. Patches of fibroin loaded with hPL were synthesized by electrospinning to take advantage of the fibrous morphology. The release kinetics of the material was characterized and tuned through the control of fibroin crystallinity. Cell viability assays, performed with primary human dermal fibroblasts, demonstrated that fibroin is able to preserve the hPL biological activity and prolong its shelf-life. The strategy of storing and preserving small active molecules within a naturally-derived, protein-based fibrous scaffold was successfully implemented, leading to the design of a biocompatible device, which can potentially simplify the storage and the application of the hPL on a human patient, undergoing medical procedures such as surgery and wound care. Statement of Significance: Human platelets lysate (hPL) is a mixture of growth factors and cytokines able to induce the regeneration of damaged tissues. This study aims at enclosing hPL in a silk fibroin electrospun matrix to expand its utilization. Silk fibroin showed the ability to preserve the hPL activity at temperature up to 60 \ub0C and the manipulation of fibroin's crystallinity provided a tool to modulate the hPL release kinetic. This entails the possibility to fabricate the hPL silk fibroin patches in advance and store them, resulting in an easy and fast accessibility and an expanded use of hPL for wound healing

mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue

Activation of non-shivering thermogenesis (NST) in brown adipose tissue (BAT) has been proposed as an anti-obesity treatment. Moreover, cold-induced glucose uptake could normalize blood glucose levels in insulin-resistant patients. It is therefore important to identify novel regulators of NST and cold-induced glucose uptake. Mammalian target of rapamycin complex 2 (mTORC2) mediates insulin-stimulated glucose uptake in metabolic tissues, but its role in NST is unknown. We show that mTORC2 is activated in brown adipocytes upon β-adrenergic stimulation. Furthermore, mice lacking mTORC2 specifically in adipose tissue (AdRiKO mice) are hypothermic, display increased sensitivity to cold, and show impaired cold-induced glucose uptake and glycolysis. Restoration of glucose uptake in BAT by overexpression of hexokinase II or activated Akt2 was sufficient to increase body temperature and improve cold tolerance in AdRiKO mice. Thus, mTORC2 in BAT mediates temperature homeostasis via regulation of cold-induced glucose uptake. Our findings demonstrate the importance of glucose metabolism in temperature regulation

Widespread association between the ericoid mycorrhizal fungus Rhizoscyphus ericae and a leafy liverwort in the maritime and sub-Antarctic

A recent study identified a fungal isolate from the Antarctic leafy liverwort Cephaloziella varians as the ericoid mycorrhizal associate Rhizoscyphus ericae. However, nothing is known about the wider Antarctic distribution of R. ericae in C. varians, and inoculation experiments confirming the ability of the fungus to form coils in the liverwort are lacking. Using direct isolation and baiting with Vaccinium macrocarpon seedlings, fungi were isolated from C. varians sampled from eight sites across a 1875-km transect through sub- and maritime Antarctica. The ability of an isolate to form coils in aseptically grown C. varians was also tested. Fungi with 98–99% sequence identity to R. ericae internal transcribed spacer (ITS) region and partial large subunit ribosomal (r)DNA sequences were frequently isolated from C. varians at all sites sampled. The EF4/Fung5 primer set did not amplify small subunit rDNA from three of five R. ericae isolates, probably accounting for the reported absence of the fungus from C. varians in a previous study. Rhizoscyphus ericae was found to colonize aseptically-grown C. varians intracellularly, forming hyphal coils. This study shows that the association between R. ericae and C. varians is apparently widespread in Antarctica, and confirms that R. ericae is at least in part responsible for the formation of the coils observed in rhizoids of field-collected C. varians

Effects of CMB temperature uncertainties on cosmological parameter estimation

We estimate the effect of the experimental uncertainty in the measurement of the temperature of the cosmic microwave background (CMB) on the extraction of cosmological parameters from future CMB surveys. We find that even for an ideal experiment limited only by cosmic variance up to l = 2500 for both the temperature and polarisation measurements, the projected cosmological parameter errors are remarkably robust against the uncertainty of 1 mK in the FIRAS instrument's CMB temperature monopole measurement. The maximum degradation in sensitivity is 20%, for the baryon density estimate, relative to the case in which the monopole is known infinitely well. While this degradation is acceptable, we note that reducing the uncertainty in the current temperature measurement by a factor of five will bring it down to the per cent level. We also estimate the effect of the uncertainty in the dipole temperature measurement. Assuming the overall calibration of the data to be dominated by the dipole error of 0.2% from FIRAS, the sensitivity degradation is insignificant and does not exceed 10% in any parameter direction.Comment: 12 pages, 2 figures, uses iopart.cls, v2: added discussion of CMB dipole uncertainty, version accepted by JCA

Constraints on non-thermal Dark Matter from Planck lensing extraction

Distortions of CMB temperature and polarization anisotropy maps caused by gravitational lensing, observable with high angular resolution and sensitivity, can be used to constrain the sterile neutrino mass, offering several advantages against the analysis based on the combination of CMB, LSS and Ly\alpha forest power spectra. As the gravitational lensing effect depends on the matter distribution, no assumption on light-to-mass bias is required. In addition, unlike the galaxy clustering and Ly\alpha forest power spectra, the projected gravitational potential power spectrum probes a larger range of angular scales, the non-linear corrections being required only at very small scales. Taking into account the changes in the time-temperature relation of the primordial plasma and the modification of the neutrino thermal potential, we compute the projected gravitational potential power spectrum and its correlation with the temperature in the presence of DM sterile neutrino. We show that the cosmological parameters are generally not biased when DM sterile neutrino is included. From this analysis we found a lower limit on DM sterile neutrino mass m_s >2.08 keV at 95% CL, consistent with the lower mass limit obtained from the combined analysis of CMB, SDSS 3D power spectrum and SDSS Ly\alpha forest power spectrum ($m_{\nu_s}>1.7$ keV). We conclude that although the information that can be obtained from lensing extraction is rather limited due to the high level of the lensing noise of Planck experiment, weak lensing of CMB offers a valuable alternative to constrain the dark matter sterile neutrino mass.Comment: 15 pages, 6 figure

Measuring primordial gravitational waves from CMB B-modes in cosmologies with generalized expansion histories

We evaluate our capability to constrain the abundance of primordial tensor perturbations in cosmologies with generalized expansion histories in the epoch of cosmic acceleration. Forthcoming satellite and sub-orbital experiments probing polarization in the CMB are expected to measure the B-mode power in CMB polarization, coming from PGWs on the degree scale, as well as gravitational lensing on arcmin scales; the latter is the main competitor for the measurement of PGWs, and is directly affected by the underlying expansion history, determined by the presence of a DE component. In particular, we consider early DE possible scenarios, in which the expansion history is substantially modified at the epoch in which the CMB lensing is most relevant. We show that the introduction of a parametrized DE may induce a variation as large as 30% in the ratio of the power of lensing and PGWs on the degree scale. We find that adopting the nominal specifications of upcoming satellite measurements the constraining power on PGWs is weakened by the inclusion of the extra degrees of freedom, resulting in a reduction of about 10% of the upper limits on r in fiducial models with no GWs, as well as a comparable increase in the error bars in models with non-zero r. Moreover, we find that the inclusion of sub-orbital CMB experiments, capable of mapping the B-mode power up to the angular scales affected by lensing, can restore the forecasted performances with a cosmological constant. Finally, we show how the combination of CMB data with Type Ia SNe, BAO and Hubble constant allows to constrain simultaneously r and the DE quantities in the parametrization we consider, consisting of present abundance and first redshift derivative of the energy density. We compare this study with results obtained using the forecasted lensing potential measurement precision from CMB satellite observations, finding consistent results.Comment: 17 pages, 9 figures, accepted for publication by JCAP. Modified version after the referee's comment

Impacts to Quail Space Use and Demographics from Oil and Gas Development

Southern Texas contains some of the last relatively unfragmented habitat for northern bobwhite (Colinus virginianus; hereafter, bobwhite) and scaled quail (Callipepla squamata) in the United States. Development of the Eagle Ford Shale hydrocarbon formation in this region could negatively impact quail and their habitat. Our objective was to examine the indirect effects of oil and gas activity (traffic and noise) on bobwhite and scaled quail on 2 private ranches in southern Texas. In 2015 and 2016, we radio-marked bobwhite and scaled quail in 2 areas where oil and gas activity was occurring (disturbed treatment) and 2 areas where little oil and gas activity occurred (undisturbed treatment). We measured vehicle passages and modeled noise propagation from oil and gas infrastructure at 2 biologically relevant frequencies (250 Hz and 1,000 Hz) in our study area to quantify oil and gas disturbance and examine its effects on quail space use (site selection and home range size) and demographics (survival, nest success, and density). Bobwhite and scaled quail selected areas 0–200 m and \u3e425 m, respectively, from the primary, high-traffic roads in the disturbed treatment. In the undisturbed treatment, bobwhite and scaled quail selected areas 0–425 m and 0–300 m from primary roads, respectively. Bobwhite and scaled quail selected areas with sound levels 0–1.6 and 0–2.2 dB above ambient levels at the 250-Hz frequency level, respectively. At 1,000 Hz, bobwhite and scaled quail selected areas with sound levels 0–2 and 0–3.2 dB above ambient levels, respectively. We found no evidence that disturbance variables affected bobwhite and scaled quail home range size, survival, or density. We found bobwhite nest success decreased as sound levels (dB) at 250 Hz increased; we found no relationship between nest success and disturbance for scaled quail, possibly as they avoided major oil and gas disturbances. In calculations of the total footprint of quail habitat loss, indirect loss due to oil and gas activity needs to be considered in addition to direct loss due to conversion of rangeland to oil and gas infrastructure