Platelet and leukocyte rich fibrin (L-PRF) applied in necrotizing Fasciitis treatment: case report: Fibrina rica em plaquetas e leucócitos (L-PRF) aplicada ao tratamento de Fasceíte necrotizante: relato de caso

The reconstruction of extensive defects on the face caused by infections is a challenge for the oral and maxillofacial surgeon. Platelet and leukocyte-rich fibrin (L-PRF) is an excellent option for rehabilitative treatment, as it contains molecular and cellular elements that induce repair, being a therapeutic biomaterial with high potential for soft tissue regeneration. This article presents the clinical case of the patient C.T.A.S., female, 21 years old, who reported multiple tooth extractions, with an evolution of 30 days of volume increase and intense algesia in the left bucal space. Diagnosing necrotizing fasciitis, the patient underwent IV antibiotic therapy and surgery to drain and debride the lesion. Postoperatively, therapy with platelet- and leukocyte-rich fibrin was initiated for tissue regeneration in the area affected by fasciitis. As an adjuvant to L-PRF membranes, low-level laser therapy and hyperbaric oxygenation sessions were performed. When using L-PRF to resolve this case, a satisfactory result was observed regarding the aesthetic-functional repair. In addition to having the advantage of being cost-effective, and having a simple protocol to be performed, L-PRF proved to be effective in promoting tissue regeneration

Perception of olive oils sensory defects using a potentiometric taste device

The capability of perceiving olive oils sensory defects and intensities plays a key role on olive oils quality grade classification since olive oils can only be classified as extra-virgin if no defect can be perceived by a human trained sensory panel. Otherwise, olive oils may be classified as virgin or lampante depending on the median intensity of the defect predominantly perceived and on the physicochemical levels. However, sensory analysis is time-consuming and requires an official sensory panel, which can only evaluate a low number of samples per day. In this work, the potential use of an electronic tongue as a taste sensor device to identify the defect predominantly perceived in olive oils was evaluated. The potentiometric profiles recorded showed that intra- and inter-day signal drifts could be neglected (i.e., relative standard deviations lower than 25%), being not statistically significant the effect of the analysis day on the overall recorded E-tongue sensor fingerprints (P-value=0.5715, for multivariate analysis of variance using Pillai's trace test), which significantly differ according to the olive oils sensory defect (P-value=0.0084, for multivariate analysis of variance using Pillai's trace test). Thus, a linear discriminant model based on 19 potentiometric signal sensors, selected by the simulated annealing algorithm, could be established to correctly predict the olive oil main sensory defect (fusty, rancid, wet-wood or winey-vinegary) with average sensitivity of 75±3% and specificity of 73±4% (repeated K-fold cross-validation variant: 4 folds×10 repeats). Similarly, a linear discriminant model, based on 24 selected sensors, correctly classified 92±3% of the olive oils as virgin or lampante, being an average specificity of 93±3% achieved. The overall satisfactory predictive performances strengthen the feasibility of the developed taste sensor device as a complementary methodology for olive oils defects analysis and subsequent quality grade classification. Furthermore, the capability of identifying the type of sensory defect of an olive oil may allow establishing helpful insights regarding bad practices of olives or olive oils production, harvesting, transport and storage.This work was financially supported by Project POCI-01–0145FEDER-006984 – Associate Laboratory LSRE-LCM, Project UID/QUI/ 00616/2013 – CQ-VR, and UID/AGR/00690/2013 – CIMO all funded by FEDER - Fundo Europeu de Desenvolvimento Regional through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT Fundação para a Ciência e a Tecnologia, Portugal. Strategic funding of UID/BIO/04469/2013 unit is also acknowledged. Nuno Rodrigues thanks FCT, POPH-QREN and FSE for the Ph.D. Grant (SFRH/BD/ 104038/2014).info:eu-repo/semantics/publishedVersio

EXPANSÃO DA SILVICULTURA DO EUCALIPTO EM ÁREAS DO CERRADO:

Considerando o avanço da cultura do eucalipto para a região do Cerrado e as controvérsias sobre as implicações positivas e negativas dessa expansão, este estudo realizou revisão narrativa de literatura de estudos empíricos recentes sobre o tema e analisou dados de publicações sobre a cultura do eucalipto, disponibilizadas por instituições oficiais especializadas, com o objetivo discorrer sobre o avanço da cultura do eucalipto, destacando os fatores condicionantes e as implicações econômicas, sociais e ambientais. Os resultados mostram que três estados (Mato Grosso do Sul, Maranhão e Tocantins) se destacam nacionalmente dentre aqueles de maior taxa de crescimento de área plantada no período de 2005 a 2021, e que o avanço do eucalipto para este bioma ocorreu devido a fatores como condições edafoclimáticas, desenvolvimento de novas mudas de plantas e disponibilidade de grandes extensões de áreas de pastagens. Se por um lado, a expansão da silvicultura do eucalipto implicou fatores econômicos positivos, por outro, potencializou problemas sociais e ambientais que, a julgar pelos estudos analisados, não compensarem os possíveis benefícios. O estudo permite reflexões sobre as implicações do avanço da cultura do eucalipto no Cerrado, trazendo evidências que podem ser melhor investigadas empiricamente por futuras específicas. Palavras-chave: Florestas; áreas plantadas no Cerrado; impactos econômicos, sociais e ambientais

Tratamento de fratura de mandíbula atrófica abordada por acesso transcervical: relato de caso: Treatment of atrophic jaw fracture approached by transcervical access: case report

O trauma buco-maxilo-facial é frequente em todo o mundo. A partir da crescente longevidade, esse tipo de acidente tem sido cada vez mais frequente em idosos. Os principais agentes etiológicos das fraturas de mandíbula, observados nessa população, são quedas, acidentes automobilísticos e as agressões. Nestes pacientes, as alterações fisiológicas e/ou anatômicas, decorrentes da senilidade tem uma influência direta na ocorrência de fraturas mandibulares em maxilares atróficos, principalmente devido à ausência de elementos dentários. Este trabalho tem como objetivo descrever um caso clínico de fratura em mandíbula atrófica em paciente geriátrico, após acidente com animal. Como forma de tratamento foi preconizado o sistema de fixação de reconstrução load-bearing, conforme a literatura atual. Conclui-se que as fraturas atróficas de mandíbula exigem um tratamento complexo, o qual apresenta bom prognóstico se bem planejado, executando as técnicas corretas, sendo fundamental que o cirurgião buco-maxilo-facial seja capacitado para este tipo de abordagem

Porphyrin–nanodiamond hybrid materials—active, stable and reusable cyclohexene oxidation catalysts

funded by FCT-Foundation for Science and Technology, I.P., under projects UIDB/00313/2020; PTDC/QUI-OUT/27996/2017 (DUALPI); POCI-01-0145-FEDER-027996; POCI-01-0145-FEDER-016387; UIDB/50006/2020 (Associate Laboratory for Green Chemistry-LAQV); MATIS (CENTRO-010145-FEDER-00014); Base Funding-UIDB/50020/2020 of the Associate Laboratory LSRE-LCM-funded by national funds through FCT/MCTES (PIDDAC); and 5625-DRI-DAAD-2020/21. SACC also acknowledges FCT Investigador FCT program (IF/01381/2013/CP1160/CT0007) and Scientific Employment Stimulus -Institutional Call (CEECINST/00102/2018). The authors also thank Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) for CEPOF 2013/07276-1, and INCT "Basic Optics and Applied to Life Sciences" (FAPESP 2014/50857-8, CNPq 465360/2014-9). A.R.L. Caires acknowledges CAPES-PrInt funding program (grant number 88887.353061/2019-00 and 88881.311921/2018-01). J.G.B. thanks the Dutch Research Council (NWO) for funding as a part of the Open Technology Programme (project number 16361). L.D. Dias thanks FAPESP for the Post-doc grant 2019/13569-8. F.M.S.R. thanks FCT for the PhD grant (PD/BD/114340/2016).The quest for active, yet “green” non-toxic catalysts is a continuous challenge. In this work, covalently linked hybrid porphyrin–nanodiamonds were prepared via ipso nitro substitution reaction and characterized by X-ray photoelectron spectroscopy (XPS), fluorescence spectroscopy, infrared spectroscopy (IR) and thermogravimetry-differential scanning calorimetry (TG-DSC). The amine-functionalized nanodiamonds (ND@NH2 ) and 2-nitro-5,10,15,20-tetra(4-trifluoromethylphenyl)porphyrin covalently linked to nanodiamonds (ND@βNH-TPPpCF3 ) were tested using Allium cepa as a plant model, and showed neither phytotoxicity nor cytotoxicity. The hybrid nanodiamond–copper(II)–porphyrin material ND@βNH-TPPpCF3-Cu(II) was also evaluated as a reusable catalyst in cyclohexene allylic oxidation, and displayed a remarkable turnover number (TON) value of ≈265,000, using O2 as green oxidant, in the total absence of sacrificial additives, which is the highest activity ever reported for said allylic oxidation. Additionally, ND@βNH-TPPpCF3-Cu(II) could be easily separated from the reaction mixture by centrifugation, and reused in three consecutive catalytic cycles without major loss of activity.publishersversionpublishe

Biocompatible Magic Sized Quantum Dots: Luminescent Markers and Probes

Nanoscience and nanobiotechnology have aroused great academic and technological interest. Works relating biomaterials at the nanoscale can reach new biotechnologies and help in the development and use of tools for bioimage and diagnosis applications. In this work we demonstrated the advantages of magic sized quantum dots as luminescent markers and probes to bioimage applications. The visualization of MSQDs bioconjugated with biological probes in cells were performed at periods greater than 2 h, and visualization with no commercial dye would not be possible. Therefore, we demonstrated that theses biocompatible nanocrystals are luminescent markers and probes to diagnosis

Análise sobre acidentes em humanos por ofídicos

The aim was to identify the medical conduct against snakebites in the literature. This is a descriptive study of a critical-reflexive nature, with a qualitative approach. Three categories were established for analysis, such as: Ophidian accidents, Medical conducts and Permanent education. It was noted the importance of recognizing snakes and knowledge of clinical management in the establishment of an appropriate medical conduct and that, for this, it is necessary for the professional to have a continuing education in their work environment. It is concluded that their accidents constitute a public health problem, with the highest number of accidents in rural areas, in patients of economically active age; regarding the anatomical part, the lower limbs were the most affected; Regarding seasonality, the months from October to December were more frequent and most patients sought immediate help, up to 3 hours after the accident. Among the accidents, the most frequent local manifestations were pain and edema. Most cases were classified as mild and the number of deaths extremely low. The results showed a lack in the taxonomic description and the scarcity of scientific materials that addressed the medical conduct to be taken in the face of accidents with snakes.Objetivou-se identificar a conduta médica frente aos acidentes ofídicos na literatura. Trata-se de um estudo descritivo de caráter crítico-reflexivo, de abordagem qualitativa. Estabeleceu-se três categorias para análise, tais quais: Acidentes ofídicos, Condutas médicas e Educação permanente. Notou-se a importância do reconhecimento das serpentes e conhecimento do manejo clínico no estabelecimento de uma conduta médica adequada e que, para isso, torna-se necessário que o profissional tenha uma educação continuada em seu ambiente laboral. Conclui-se que seus acidentes constituem um problema de saúde pública, sendo o maior número de acidentes na zona rural, em pacientes com idade economicamente ativa; com relação a parte anatômica, os membros inferiores foram os mais acometidos; com relação a sazonalidade, os meses de outubro a dezembro tiveram maior frequência e a maioria dos pacientes procuraram socorro imediato, até 3 horas após o acidente. Dentre os acidentes, as manifestações locais mais frequentes foram dor e edema. A maioria dos casos foram classificados como leves e o número de óbito extremamente baixos. Os resultados evidenciaram carência na descrição taxonômica e a escassez de materiais científicos que abordavam sobre as condutas médicas a serem tomadas frente aos acidentes com serpentes das serpentes

Análise sobre acidentes em humanos por ofídicos

The aim was to identify the medical conduct against snakebites in the literature. This is a descriptive study of a critical-reflexive nature, with a qualitative approach. Three categories were established for analysis, such as: Ophidian accidents, Medical conducts and Permanent education. It was noted the importance of recognizing snakes and knowledge of clinical management in the establishment of an appropriate medical conduct and that, for this, it is necessary for the professional to have a continuing education in their work environment. It is concluded that their accidents constitute a public health problem, with the highest number of accidents in rural areas, in patients of economically active age; regarding the anatomical part, the lower limbs were the most affected; Regarding seasonality, the months from October to December were more frequent and most patients sought immediate help, up to 3 hours after the accident. Among the accidents, the most frequent local manifestations were pain and edema. Most cases were classified as mild and the number of deaths extremely low. The results showed a lack in the taxonomic description and the scarcity of scientific materials that addressed the medical conduct to be taken in the face of accidents with snakes.Objetivou-se identificar a conduta médica frente aos acidentes ofídicos na literatura. Trata-se de um estudo descritivo de caráter crítico-reflexivo, de abordagem qualitativa. Estabeleceu-se três categorias para análise, tais quais: Acidentes ofídicos, Condutas médicas e Educação permanente. Notou-se a importância do reconhecimento das serpentes e conhecimento do manejo clínico no estabelecimento de uma conduta médica adequada e que, para isso, torna-se necessário que o profissional tenha uma educação continuada em seu ambiente laboral. Conclui-se que seus acidentes constituem um problema de saúde pública, sendo o maior número de acidentes na zona rural, em pacientes com idade economicamente ativa; com relação a parte anatômica, os membros inferiores foram os mais acometidos; com relação a sazonalidade, os meses de outubro a dezembro tiveram maior frequência e a maioria dos pacientes procuraram socorro imediato, até 3 horas após o acidente. Dentre os acidentes, as manifestações locais mais frequentes foram dor e edema. A maioria dos casos foram classificados como leves e o número de óbito extremamente baixos. Os resultados evidenciaram carência na descrição taxonômica e a escassez de materiais científicos que abordavam sobre as condutas médicas a serem tomadas frente aos acidentes com serpentes das serpentes

EVALUATION OF SORGHUM SILAGE WITH ADDITIVES – ALTERATIONS IN DRY MATTER, FIBROUS FRACTIONS AND “IN VITRO” DRY MATTER DIGESTIBILITY

This work evaluated the fermentation pattern of sorghum silage hybrid BR 601 with and without additives. At ensiling time, silages were enriched with the additives, adopting the following treatments: silages without additive (control), with 0.5% urea, 0.5% limestone, 0.5% urea plus limestone and bacterial inoculant. The materials were stored into PVC silos, opened after 1, 3, 5, 7, 14, 28 and 56 days of ensiling. Alterations were determined in dry matter (DM), neutral detergent fiber (NDF), hemicellulose, cellulose, acid detergent fiber (ADF) and lignin contents and in vitro dry matter digestibility (IVDMD). A completely randomized experimental design was used, in a 5 x 7 factorial scheme, and the means were compared by the SNK test at 5% probability. The silage enriched with urea plus limestone showed highest loss of DM. No difference was observed between silage with additives and control regarding NDF, hemicellulose, cellulose and DMIVD. The additives promoted changes in the ADF and lignin contents

Effect of Metabolic Syndrome on Parkinson’s Disease: A Systematic Review

Evidence shows that metabolic syndrome (MS) is associated with a greater risk of developing Parkinson’s disease (PD) because of the increase in oxidative stress levels along with other factors such as neuroinflammation and mitochondrial dysfunction. However, because some studies have reported that MS is associated with a lower risk of PD, the relationship between MS and PD should be investigated. This study aimed to investigate the effect of MS on PD. Two authors searched five electronic databases, namely, MEDLINE, PubMed, Scopus, PsycINFO, Web of Science, and Science Direct, for relevant articles between September and October 2020. After screening the title and abstract of all articles, 34 articles were selected for full-text review. Finally, 11 articles meeting the eligibility criteria were included in the study. The quality of articles was critically evaluated using the Joanna Briggs Institute. Overall, we evaluated data from 23,586,349 individuals (including healthy individuals, with MS and PD) aged 30 years or more. In cohort studies, the follow-up period varied between 2 and 30 years. MS contributed considerably to the increase in the incidence of PD. In addition, obesity, a component of MS, alone can increase the probability of developing neurodegenerative diseases. However, despite few studies on MS and PD, changes in cognitive function and more rapid progression of PD disease has been documented in patients with MS using methods commonly used in research