Estudo da biodiversidade de estirpes vínicas de S. cerevisiae

Mestrado em BioquímicaAs fermentações vínicas são constituídas por consórcios microbianos, com diferentes espécies e estirpes de leveduras, onde a Saccharomyces cerevisiae tem um papel fundamental. Assim é importante avaliar a biodiversidade intrínseca das estirpes de S. cerevisiae, por forma a explorar o seu potencial biotecnológico. O objetivo deste trabalho foi estudar a biodiversidade fenotípica e metabólica de S. cerevisiae, com especial enfoque no seu potencial enológico. Para tal, isolaram-se leveduras vínicas endógenas de 4 regiões Demarcadas portuguesas: Alentejo, Bairrada, Douro e Dão. Isolaram-se 1260 leveduras, das quais 883 eram S. cerevisiae. Relativamente à caracterização fenotípica, avaliou-se a capacidade de crescimento de 313 estirpes de S. cerevisiae em 37 condições. As condições de crescimento foram selecionadas por forma a expor a variabilidade intrínseca de S. cerevisiae, e incluíram ainda fenótipos relacionados com o potencial enológico, nomeadamente o crescimento a diferentes temperaturas e na presença de etanol, a produção de H2S, e a tolerância ao cobre e ao dióxido de enxofre. Este estudo evidenciou a existência de 292 fenótipos distintos entre a população estudada. A caracterização metabólica foi focada na análise de metabolitos voláteis produzidos por um conjunto de 4 estirpes de S. cerevisiae: 2 isolados vínicos e 2 estirpes comerciais amplamente utilizadas pela indústria. A análise foi realizada por HS-SPME-GC×GC-ToFMS, onde se identificaram um total de 218 metabolitos, pertencentes a 15 famílias, que são diferencialmente produzidos pelas estirpes analisadas. Quer os álcoois quer os ésteres foram as famílias com maior número de compostos identificados, no entanto foram os compostos terpénicos que apresentaram maior variabilidade inter-estirpe. A análise compreensiva da variabilidade intrinseca de S. cerevisiae agora realizada revelou uma elevada biodiversidade. Além disso, identificou-se um metaboloma volátil de S. cerevisiae até agora desconhecido e que demonstra a importância da continuidade do seu estudo em maior profundidade.Wine fermentation is dependent upon a microbial consortium, with different species and strains of yeasts, which Saccharomyces cerevisiae has a fundamental role. Thus, it is important to evaluate the intrinsic biodiversity of these yeasts to explore their biotechnological potential. The aim of this work was to study phenotypic and metabolic biodiversity of S. cerevisiae, and its oenological potential. For that, S. cerevisiae strains, from Alentejo, Bairrada, Douro and Dão Portuguese Appellations were isolated. A total of 1260 wine yeasts were isolated of which 883 belong to the S. cerevisiae specie. For the phenotypic characterization, we assessed the growth capacity of 313 strains of S. cerevisiae under 37 different conditions. These growth conditions were selected in order to expose the intrinsic variability of S. cerevisiae, and also included phenotypes related to its oenological potential, particularly the growth at different temperatures and in the presence of ethanol, the H2S production, and the tolerance to copper and sulfur dioxide. This study revealed the existence of 292 distinct phenotypes within the studied population. The metabolic characterization was focused on the analysis of volatile metabolites produced by a set of 4 strains of S. cerevisiae: 2 isolates and 2 commercial strains widely used by the industry. The analysis was performed by HSSPME- GC×GC-TOFMS, where a total of 218 metabolites, belonging to 15 families, were identified and were differentially produced by studied strains. Alcohols and esters were the families with the largest number of identified compounds; whereas the terpenic compounds were those that showed the highest inter-strain variability. Indeed, the comprehensive analysis of the intrinsic variability of S. cerevisiae has unveiled a surprisingly high biodiversity. Moreover, we have identified a yet unknown volatile metabolome of S. cerevisiae, which would be of great importance to continue studying

Family care in advanced COPD: Perceived difficulties and expectations of support from services

Chronic Obstructive Pulmonary Disease (COPD) is an incapacitating, highly prevalent disease which often requires intensive support from patient's family members. However, knowledge about the difficulties experienced by family members in their caregiving role remains scarce. This study aimed to explore the difficulties experienced by family carers, and their expectations towards social and health support services. A qualitative, cross-sectional study was conducted with 21 family carers of COPD outpatients at advanced grades (GOLD 3 and 4). Semi-structured interviews were performed to collect data. Participants were mostly female (n=20), with a mean age of 60.90±12.35 years old, spouses (n=12) and caring for more than 4 years (n=18). All interviews were audiorecorded, transcribed and submitted to content analysis by 2 independent judges. The major difficulties reported are related to: i) provide support in basic [washing (n=6), dressing (n=2)], and in instrumental activities of daily living [preparing meals (n=4)]; ii) communication with patient (n=2); and iii) restrictions in social activities (n=2); Six carers did not identify any difficulty. Most of participants (n=12) could not identify how formal support services could help them. Only 3 participants reported the need of help to provide support in activities of daily living. Carers reported several difficulties in their caregiving role. However, they were unable to identify how social and health services could support them. The lack of information about community resources might explain these results. Strategies towards effective flow of information must be addressed in order to prevent caregivers' burden.publishe

Anxiety and depression at early COPD: (dis)Similarities Between Patients And Their Family Caregivers

Sem resumo disponível.publishe

A relação entre o género e motivação para a prática de atividades desportivas

O objetivo do presente estudo foi avaliar a motivação para o envolvimento em atividades desportivas em função do género. A amostra foi composta por 36 clientes de um ginásio divididos por género (n=18 masculinos 34.83 ± 17.16 anos; n=18 femininos 29.72 ± 11.24 anos). Foi utilizado o Questionário de Motivação para as Atividades Desportivas (Serpa e Frias, 1990). Após uma informação inicial dos propósitos da investigação e a obtenção do consentimento por parte dos encarregados de educação dos indivíduos menores foram aplicados os questionários, garantindo o anonimato e a confidencialidade das respostas. Os questionários foram aplicados pela equipa de investigação supervisionada por um especialista em Ciências do Desporto antes dos treinos de modo a evitar diferentes estados emocionais (fadiga, cansaço, motivação). Observaram-se diferenças significativas entre géneros nos fatores “estatuto”, “competição” e “afiliação geral”, com o género masculino a evidenciar valores superiores ao género feminino nestes fatores específicos, o que se relaciona com uma motivação mais centrada no ego, É fundamental compreender os motivos que conduzem os praticantes à prática de atividades desportivas AD no sentido de procurar que as atividades vão ao encontro do público-alvo e este adira às mesmas e se mantenha envolvido e motivado ao longo do tempo.The aim of this study was to evaluate the motivation for involvement in sport activities by gender. The sample comprised 36 clients of a gym divided by gender (n = 18 male 34.83 ± 17.16, years; n = 18 female, 29.72 ± 24.11, years). The Questionnaire for Motivation for Sport Activities (Serpa & Frias, 1990) was used. After a initial information related to the research purpose and consent of the guard-ians of the younger individuals obtained the questionnaires were applied, it was guaranteed anonymity and confidentiality of the answers. Questionnaires were administered by the research team supervised by a specialist in Sports Science before training to avoid different emotional states (fatigue, tiredness, motivation). It was observed significant gender differences in factors "status", "competition" and "general membership", with male evidencing high values comparatively to female in this specific factors, which relates to a more focused motivation in the ego. It is essential to understand the reasons that lead practitioners to involve in sport activities with the purpose of search of activities which target the audience and these adhere and remain engaged and motivated throughout time

Análisis acústico de los sonidos de la deglución con el uso de Sonar Doppler en bebés prematuros durante la transición alimentaria

Objective: To define the acoustic parameters and the feasibility of using swallowing acoustic analysis as an auxiliary method for the transition from nasogastric or orogastric tube feeding to oral breastfeeding. Methodology: A cross-sectional study, which consisted of the following steps: 1. Data collection: Thirty-two newborns participated in this study (16 preterm and 16 full-term); 2. Clinical speech-language pathology evaluation: Clinical data were collected using Sonar Doppler used to assess the readiness of preterm infants to start oral feeding and swallowing sounds. Swallowing sounds were captured during breastfeeding and analyzed according to the frequency, intensity, and time of swallowing, as well as the pause time between swallows and the number of swallows. Results: The control and study groups presented significant differences in the mean wave time and swallowing per minute variables. Conclusion: Sonar Doppler, as an instrument used to capture sounds, is a viable auxiliary resource to evaluate the transition from nasogastric or orogastric tube to oral breastfeeding.info:eu-repo/semantics/publishedVersio

Relatório do workshop com representantes de associações científicas

O Workshop SOCSCI decorreu no dia 26 de abril de 2012 no Instituto de Ciências Sociais da Universidade de Lisboa. Este evento está enquadrado no projeto SOCSCI – Sociedades científicas na ciência contemporânea financiado pela Fundação para a Ciência e a Tecnologia (PTDC/CS-ECS/101592/2008), em curso no Instituto de Ciências Sociais da Universidade de Lisboa, em colaboração com o SOCIUS-ISEG e CIES-IUL. Este projeto tem por objetivo compreender que papel desempenham as sociedades científicas na ciência contemporânea em Portugal. Organizado durante a fase final do projeto, este workshop teve o duplo objetivo de dar a conhecer os principais resultados às associações que colaboraram no processo de investigação e de fornecer um espaço de discussão destes resultados e de troca de experiências entre os participantes Neste relatório serão apresentadas a estrutura de organização do evento, um resumo das intervenções dos participantes e as principais conclusões retiradas da discussão

Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene

Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progenitors of cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, and phylogeny. A RNA-seq expression analysis in different Arachis species showed that the majority of these expansins are modulated in response to diverse stresses such as water deficit, rootknot nematode (RKN) infection, and UV exposure, with an expansin-like B gene (AraEXLB8) displaying a highly distinct stress-responsive expression profile. Further analysis of the AraEXLB8 coding sequences showed high conservation across the Arachis genotypes, with eight haplotypes identified. The modulation of AraEXLB8 expression in response to the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in situ hybridization revealed transcripts in different root tissues according to the stress imposed. The overexpression of AraEXLB8 in soybean (Glycine max) composite plants remarkably decreased the number of galls in transformed hairy roots inoculated with RKN. This study improves the current understanding of the molecular evolution, divergence, and gene expression of expansins in Arachis, and provides molecular and functional insights into the role of expansin-like B, the less-studied plant expansin subfamily

CLÍNICA AMPLIADA À ESCOLA INCLUSIVA DURANTE A PANDEMIA DA COVID-19: RELATO DE EXPERIÊNCIA DE ESTÁGIO PROFISSIONALIZANTE EM PSICOLOGIA DA PUC MINAS BETIM

Em março de 2020 a comunidade escolar do país entrou em quarentena para evitar a proliferação do coronavírus. O isolamento social prolongado exigiu que a formação em Psicologia incluísse novas formas de transmissão do saber e de prestação de serviços, usando das tecnologias digitais. O estágio profissionalizante da ênfase voltada ao estudo e as práticas da Psicologia nas “Políticas Sociais” do curso de psicologia da PUC-MG unidade de Betim, tem uma parceria como Centro de Referência e Apoio à Educação Inclusiva Rafael Veneroso, visando qualificar as(os) professores das escolas da rede municipal para tratar da inclusão usando de metodologias lúdicas e coletivas. O presente artigo visa apresentar o que pudemos fazer de forma remota para não deixarmos de prestar nosso serviço a rede municipal da cidade de Betim

EAIR 41st Annual Forum in Leiden, The Netherlands 25 till 28 August 2019

Trabalho apresentado em EAIR 41st Annual Forum, 25-28 agosto 2019, Leiden, Países Baixos.Polytechnic Institutes in Portugal: research on the impact of twelve institutes on the local economy Higher Education Institutions are recognized as important actors in regional development. The Portuguese higher education system comprises both Universities and Polytechnic Institutes, which face an increasing pressure to demonstrate that their presence has an impact on the surrounding communities contributing to their economic development. This paper presents the estimation of the economic impact of twelve Polytechnic Institutes, located in quite diverse regions, based on a shared model so that comparisons have a collective framework of analysis. The main results obtained show that the economic impact ranged from 1.8% to 10.6% of the local GDP and that these Institutes are major local employers.info:eu-repo/semantics/publishedVersio