Antiproliferative activity of flavonoids from croton Sphaerogynus baill. (euphorbiaceae)

Croton sphaerogynus is a shrub from the Atlantic Rain Forest in southeastern Brazil. A lyophilized crude EtOH extract from leaves of C. sphaerogynus, obtained by maceration at room temperature (seven days), was suspended in methanol and partitioned with hexane. The purified MeOH phase was fractionated over Sephadex LH-20 yielding five fractions (F1-F5) containing flavonoids, as characterized by HPLC-DAD and HPLC-MS analyses. The antiproliferative activity of the crude EtOH extract, MeOH and hexane phases, and fractions F1-F5 was evaluated on in vitro cell lines NCI-H460 (nonsmall cell lung), MCF-7 (breast cancer), and U251 (glioma). The MeOH phase showed activity (mean log GI(50) 0.54) higher than the hexane phase and EtOH extract (mean log GI(50) 1.13 and 1.19, resp.). F1 exhibited activity against NCI-H460 (nonsmall cell lung) (GI(50) 1.2 mu g/mL), which could be accounted for the presence of flavonoids and/or diterpenes. F4 showed moderate activity (mean log GI(50) 1.05), while F5 showed weak activity (mean log GI(50) 1.36). It is suggested that the antiproliferative activity of the crude EtOH extract and MeOH phase is accounted for a synergistic combination of flavonoids and diterpenes2015COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informação2012/10079-

Antiproliferative Activity Of Flavonoids From Croton Sphaerogynus Baill. (euphorbiaceae).

Croton sphaerogynus is a shrub from the Atlantic Rain Forest in southeastern Brazil. A lyophilized crude EtOH extract from leaves of C. sphaerogynus, obtained by maceration at room temperature (seven days), was suspended in methanol and partitioned with hexane. The purified MeOH phase was fractionated over Sephadex LH-20 yielding five fractions (F1-F5) containing flavonoids, as characterized by HPLC-DAD and HPLC-MS analyses. The antiproliferative activity of the crude EtOH extract, MeOH and hexane phases, and fractions F1-F5 was evaluated on in vitro cell lines NCI-H460 (nonsmall cell lung), MCF-7 (breast cancer), and U251 (glioma). The MeOH phase showed activity (mean log GI50 0.54) higher than the hexane phase and EtOH extract (mean log GI50 1.13 and 1.19, resp.). F1 exhibited activity against NCI-H460 (nonsmall cell lung) (GI50 1.2 μg/mL), which could be accounted for the presence of flavonoids and/or diterpenes. F4 showed moderate activity (mean log GI50 1.05), while F5 showed weak activity (mean log GI50 1.36). It is suggested that the antiproliferative activity of the crude EtOH extract and MeOH phase is accounted for a synergistic combination of flavonoids and diterpenes.201521280

Perfil epidemiológico de casos notificados da doença falciforme no Ceará / Epidemiological profile of notified cases of sickle cell disease in Ceará

Introdução: A doença falciforme é uma das doenças mais comuns no mundo, é hereditária e caracteriza-se pela presença da hemoglobina S. O Programa Nacional de Triagem Neonatal tem proporcionado o diagnóstico precoce da doença falciforme em neonatos, contribuindo para melhor qualidade de vida dos casos confirmados. Objetivo: descrever o perfil epidemiológico quanto aos aspectos sócio demográficos e clínicos da doença falciforme. Métodos: Trata-se de estudodescritivo com dados secundários registrados de 1994 a 2018, que incluíram dados de prontuários de 668 pacientes com a doença falciforme no Estado do Ceará entre os anos de 1964 e 2018, por meio do sistema de informação de três unidades de referência, com atendimento do Sistema Único de Saúde. Realizou-se a descrição das variáveis por meio do cálculo das médias, medianas, frequência absoluta e relativa. Resultados: Observou-se que a maioria dos casos foi encontrado em Fortaleza (74,4%) com atendimento na hemorrede (51,5%). As mulheres foram maioria (53%), sendo a cor parda a de maior predominância (69%). Os dados clínicos da maioria dos casos apresentaram prevalência de hemoglobinopatia SS (HbSS) (69,9%), necessidade de transfusões (53,3%), crise álgica (54,9%), pneumonia (17,1%) e utilização de ácido fólico (89,1%). Conclusão: As mulheres e pessoas de cor parda representaram a maioria da amostra com anemia falciforme ao longo de meio século. Observou-se predominância da Hemoglobinopatia do tipo HbSS e ocorrência de transfusões, sem informações quanto ao número de sessões realizadas

Análise econômica do cultivo de açaizeiro (Euterpe oleracea Mart.) irrigado no nordeste paraense.

Análise econômica do cultivo de açaizeiro (Euterpe oleracea Mart.) plantado em terra fi rme é essencial devido ao alto investimento em irrigação. O objetivo deste trabalho foi avaliar a viabilidade econômica de uma plantação comercial de açaizeiro irrigado por microaspersão. A plantação, de 16 ha e com plantas de 8 anos, estava localizada no município de Igarapé-Açu, estado do Pará, e foi instalada e conduzida segundo práticas regionais de cultivo. Para avaliar a viabilidade, os seguintes índices econômi-cos foram calculados: valor presente líquido (VPL), valor presente líquido anualizado (VPLa), índice be-nefício-custo (IBC), retorno adicional sobre investimento (Roia), taxa interna de retorno (TIR) e payback descontado. O fluxo de caixa foi dimensionado para um período de 20 anos, e a taxa mínima de atrativi-dade (TMA) foi fi xada em 6,93%. Para a estrutura de produção avaliada na propriedade onde a plantação se encontrava, atestou-se elevada viabilidade econômica da atividade, com riqueza acrescentada em VPL da ordem de R$983.394,31 para um horizonte de 20 anos de projeto. A atividade de açaizeiro irrigado por microaspersão é viável, com rendimento líquido de R$ 3.860,40/ha e ganho real de 13,07%, descontada a taxa real de juros empregada no cálculo do VPL.Edição dos anais do 58º Congresso da Sociedade Brasileira de Economia, Administração e Sociologia Rural (Sober), Manaus, 2020

Predicting sepsis severity at first clinical presentation:The role of endotypes and mechanistic signatures

BACKGROUND: Inter-individual variability during sepsis limits appropriate triage of patients. Identifying, at first clinical presentation, gene expression signatures that predict subsequent severity will allow clinicians to identify the most at-risk groups of patients and enable appropriate antibiotic use. METHODS: Blood RNA-Seq and clinical data were collected from 348 patients in four emergency rooms (ER) and one intensive-care-unit (ICU), and 44 healthy controls. Gene expression profiles were analyzed using machine learning and data mining to identify clinically relevant gene signatures reflecting disease severity, organ dysfunction, mortality, and specific endotypes/mechanisms. FINDINGS: Gene expression signatures were obtained that predicted severity/organ dysfunction and mortality in both ER and ICU patients with accuracy/AUC of 77–80%. Network analysis revealed these signatures formed a coherent biological program, with specific but overlapping mechanisms/pathways. Given the heterogeneity of sepsis, we asked if patients could be assorted into discrete groups with distinct mechanisms (endotypes) and varying severity. Patients with early sepsis could be stratified into five distinct and novel mechanistic endotypes, named Neutrophilic-Suppressive/NPS, Inflammatory/INF, Innate-Host-Defense/IHD, Interferon/IFN, and Adaptive/ADA, each based on ∼200 unique gene expression differences, and distinct pathways/mechanisms (e.g., IL6/STAT3 in NPS). Endotypes had varying overall severity with two severe (NPS/INF) and one relatively benign (ADA) groupings, consistent with reanalysis of previous endotype studies. A 40 gene-classification tool (accuracy=96%) and several gene-pairs (accuracy=89–97%) accurately predicted endotype status in both ER and ICU validation cohorts. INTERPRETATION: The severity and endotype signatures indicate that distinct immune signatures precede the onset of severe sepsis and lethality, providing a method to triage early sepsis patients

Copolymer-induced stabilizing effect of highly swollen hexagonal mesophases

We show quantitatively that tiny amounts of copolymer that decorate a oil/water interfaces can greatly enhance the stability of swollen surfactant hexagonal phases, comprising oil tubes regularly arranged in a water matrix. Such soft composite materials, whose both radius of the tubes and water channel between the tubes can be controlled independently over large ranges, offer a potential interest for the synthesis of mesoporous materials

Ancestry of the Brazilian TP53 c.1010G>A (p.Arg337His, R337H) founder mutation : clues from haplotyping of short tandem repeats on Chromosome 17p

Rare germline mutations in TP53 (17p13.1) cause a highly penetrant predisposition to a specific spectrum of early cancers, defining the Li-Fraumeni Syndrome (LFS). A germline mutation at codon 337 (p.Arg337His, c1010G>A) is found in about 0.3% of the population of Southern Brazil. This mutation is associated with partially penetrant LFS traits and is found in the germline of patients with early cancers of the LFS spectrum unselected for familial his- tory. To characterize the extended haplotypes carrying the mutation, we have genotyped 9 short tandem repeats on chromosome 17p in 12 trios of Brazilian p.Arg337His carriers. Results confirm that all share a common ancestor haplotype of Caucasian/Portuguese-Ibe- ric origin, distant in about 72–84 generations (2000 years assuming a 25 years intergenera- tional distance) and thus pre-dating European migration to Brazil. So far, the founder p. Arg337His haplotype has not been detected outside Brazil, with the exception of two resi- dents of Portugal, one of them of Brazilian origin. On the other hand, increased meiotic recombination in p.Arg337His carriers may account for higher than expected haplotype diversity. Further studies comparing haplotypes in populations of Brazil and of other areas of Portuguese migration are needed to understand the historical context of this mutation in Brazil.This study was funded by grant # 478430/2012-4 from CNPq (RFA MCT/CNPq - No 14/2012; Universal), Brazil.We would like to thank UFRGS, UFPA, AC Camargo, HC Barretos and University of Minho for their support during this work

Gestão por competências em organizações de governo

Ao longo dos últimos anos vem se ampliando os espaços e o interesse, no meio acadêmico e empresarial, pelas discussões sobre uso da abordagem da competência como marco importante para as atividades de gestão de recursos humanos. A difusão dessa abordagem entre as organizações públicas brasileiras é, entretanto, bem mais recente, e conta, ainda hoje, com um número insuficiente de estudos e publicações. É esta a razão da importância e pertinência da publicação do livro " Gestão por competências em organizações de governo"Número de páginas: 100 p.Gestão de PessoasISBN 85-256-0046-

Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli

Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts.  Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins.  Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets