VALIDAÇÃO E APLICAÇÃO DE MÉTODO PARA ANÁLISE DE PESTICIDAS EM ÁGUA PARA CONSUMO HUMANO DE DOURADOS (MS) POR CLAE/UV E CG/DTE

O uso inadequado de pesticidas na agricultura tem sido apontado como fonte de risco para a saúde humana e para o ambiente. Considerando que os recursos hídricos são os principais destinos desses compostos após a aplicação, este trabalho apresenta a otimização e validação de dois métodos analíticos simples e eficientes para a determinação de pesticidas em águas superficiais e subterrâneas. Foram selecionados os pesticidas mais aplicados no município de Dourados, (Mato Grosso – Brasil) com intensa atividade agrícola. Efetuou-se a pré-concentração por extração em fase sólida com cartucho C18 (500 mg) e eluição com metanol para as amostras analisadas por cromatografia a líquido de alta eficiência com detector espectrofotométrico na região do ultravioleta (CLAE/UV) (2,4-D e 2,4-DCF) e com acetato de etila:diclorometano (1:1, v/v) para as analisadas por cromatografia a gás com detector termiônico específico (CG/DTE) (atrazina, DIA, DEA, trifluralina e parationa metílica). Os métodos apresentaram exatidão (76-107%) e precisão (<12%) satisfatórias para as substâncias nos níveis de fortificação selecionados, exceto para DIA (<51%), assim como para o estudo de estabilidade dos pesticidas (-20ºC por até 21 dias). Os limites de quantificação dos métodos (0,22 - 0,48 µg L-1) estão de acordo com a legislação brasileira vigente para pesticidas em água. Embora somente o 2,4-D tenha sido detectado em dois pontos de coleta no período estudado, alerta-se para a necessidade de avaliação sistemática da presença de pesticidas em água para consumo humano, particularmente, em regiões com intensa atividade agrícola. Tal monitoramento pode fornecer subsídios para políticas públicas ambientais

Projeto de Iluminação com Sistema Fotovoltaico Isolado para Ônibus Food Truck

As for the current society, there’s a search for renewable energy sources, and given the situation, this project was brought up as a way to provide energy to an isolated system that has not got a way of generating energy for itself, automatizing this energy-give process. Therefore the objective of this project was to develop a photovoltaic system that has the autonomy to provide energy to itself, and thy system shall be allocated onto a food truck. The system was fixed into a structure on top of the food truck, the structure which can be detached if the food truck ever needs to move. A few other components could be found inside of the food truck. This system was implanted into IFSC Campus Xanxerê’s container, simulating a food truck. Developing thy project helped to show how IFSC Campus Xanxerê is committed with sustainable development and with the communit

Antimicrobianos Veterinários: Uso na Avicultura e Ocorrência em Cama de Frango

The concern with the entry and exit of veterinary antimicrobials in the environment is rising, taking into account the expressive increase of intensive systems of poultry farming. The presence of several classes of antimicrobials in poultry litter, material with a varying composition which is distributes over the floor of sheds, and that has been thrown in agricultural soil after prohibition of its use in animal feed, has contributed to increase the presence of these compounds in soil and surface water. Although Brazil is the third largest producer of chicken meat, only few studies that investigate the presence of these compounds in poultry litter and in the environment have been described. Considering these aspects, this paper aims to present a discussion about poultry litter as a source of antimicrobials to the environment, as well as levels of these compounds found in other matrices, based on a literature research performed between 1999 to 2013 in the databases Web of Science, SciFinder, and Scopus. Gaps in this knowledge area are pointed out as suggestions for future studies, which could provide tools to improve decision for treatment and/or final disposal of waste generated by poultry farming.A preocupação com a entrada e destino de antimicrobianos veterinários no ambiente é crescente, considerando o aumento expressivo da criação intensiva de frango de corte. A presença de diversas classes de antimicrobianos em cama de frango, material de composição diversa distribuído sobre o piso de galpões, e que tem sido lançado no solo agrícola após proibição do seu uso na ração animal, tem contribuído para aumentar a presença desses compostos em solo e água superficial. O Brasil, embora seja o terceiro maior produtor mundial de carne de frango, apresenta poucos trabalhos que investigam a presença de antimicrobianos veterinários em cama de frango e no ambiente. Considerando esses aspectos, este trabalho apresenta uma discussão sobre a entrada de antimicrobianos no ambiente pela cama de frango, assim como níveis encontrados em outras matrizes, tendo como base um levantamento bibliográfico realizado no período de 1999 a 2013 nas bases de dados Web of Science, SciFinder e Scopus. Apontam-se lacunas ainda existentes nessa área e que merecem atenção em estudos futuros para fornecer ferramentas que visem à melhor tomada de decisão para tratamento e/ou destinação final dos resíduos gerados pela avicultura

Impact of periprocedural myocardial injury after transcatheter aortic valve implantation on long-term mortality: a meta-analysis of Kaplan-Meier derived individual patient data

BackgroundPeriprocedural myocardial injury (PPMI) frequently occurs after transcatheter aortic valve implantation (TAVI), although its impact on long-term mortality is uncertain.MethodsWe performed a pooled analysis of Kaplan-Meier-derived individual patient data to compare survival in patients with and without PPMI after TAVI. Flexible parametric models with B-splines and landmark analyses were used to determine PPMI prognostic value. Subgroup analyses for VARC-2, troponin, and creatine kinase-MB (CK-MB)-defined PPMI were also performed.ResultsEighteen observational studies comprising 10,094 subjects were included. PPMI was associated with lower overall survival (OS) after two years (HR = 1.46, 95% CI 1.30–1.65, p &lt; 0.01). This was also observed when restricting the analysis to overall VARC-2-defined PPMI (HR = 1.23, 95% CI 1.07–1.40, p &lt; 0.01). For VARC-2 PPMI criteria and VARC-2 troponin-only, higher mortality was restricted to the first 2 months after TAVI (HR = 1.64, 95% CI 1.31–2.07, p &lt; 0.01; and HR = 1.32, 95% CI 1.05–1.67, p = 0.02, respectively), while for VARC-2 defined CK-MB-only the increase in mortality was confined to the first 30 days (HR = 7.44, 95% CI 4.76–11.66, p &lt; 0.01).ConclusionPPMI following TAVI was associated with lower overall survival compared with patients without PPMI. PPMI prognostic impact is restricted to the initial months after the procedure. The analyses were consistent for VARC-2 criteria and for both biomarkers, yet CK-MB was a stronger prognostic marker of mortality than troponin

Taxonomy and pathology of Togninia (Diaporthales) and its Phaeoacremonium anamorphs.

The genus Togninia (Diaporthales, Togniniaceae) is here monographed along with its Phaeoacremonium (Pm.) anamorphs. Ten species of Togninia and 22 species of Phaeoacremonium are treated. Several new species of Togninia (T.) are described, namely T. argentinensis (anamorph Pm. argentinense), T. austroafricana (anamorph Pm. austroafricanum), T. krajdenii, T. parasitica, T. rubrigena and T. viticola. New species of Phaeoacremonium include Pm. novae-zealandiae (teleomorph T. novae-zealandiae), Pm. iranianum, Pm. sphinctrophorum and Pm. theobromatis. Species can be identified based on their cultural and morphological characters, supported by DNA data derived from partial sequences of the actin and ß-tubulin genes. Phylogenies of the SSU and LSU rRNA genes were used to determine whether Togninia has more affinity with the Calosphaeriales or the Diaporthales. The results confirmed that Togninia had a higher affinity to the Diaporthales than the Calosphaeriales. Examination of type specimens revealed that T. cornicola, T. vasculosa, T. rhododendri, T. minima var. timidula and T. villosa, were not members of Togninia. The new combinations Calosphaeria cornicola, Calosphaeria rhododendri, Calosphaeria transversa, Calosphaeria tumidula, Calosphaeria vasculosa and Jattaea villosa are proposed. Species of Phaeoacremonium are known vascular plant pathogens causing wilting and dieback of woody plants. The most prominent diseases in which they are involved are Petri disease and esca, which occur on grapevines and are caused by a complex of fungi, often including multiple species of Phaeoacremonium. Various Phaeoacremonium species are opportunistic fungi on humans and cause phaeohyphomycosis. The correct and rapid identification of Phaeoacremonium species is important to facilitate the understanding of their involvement in plant as well as human disease. A rapid identification method was developed for the 22 species of Phaeacremonium. It involved the use of 23 species-specific primers, including 20 primers targeting the ß-tubulin gene and three targeting the actin gene. These primers can be used in 14 multiplex reactions. Additionally, a multiple-entry electronic key based on morphological, cultural and ß-tubulin sequence data was developed to facilitate phenotypic and sequence-based species identification of the different Phaeoacremonium species. Separate dichotomous keys are provided for the identification of the Togninia and Phaeoacremonium species. Keys for the identification of Phaeoacremonium-like fungi and the genera related to Togninia are also provided. The mating strategy of several Togninia species was investigated with ascospores obtained from fertile perithecia produced in vitro. Togninia argentinensis and T. novae-zealandiae have homothallic mating systems, whereas T. austroafricana, T. krajdenii, T. minima, T. parasitica, T. rubrigena and T. viticola were heterothallic.

Detection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology

Background: ALK-rearranged lung cancers exhibit specific pathologic and clinical features and are responsive to anti-ALK therapies. Therefore, the detection of ALK-rearrangement is fundamental for personalized lung cancer therapy. Recently, new molecular techniques, such as NanoString nCounter, have been developed to detect ALK fusions with more accuracy and sensitivity. Methods: In the present study, we intended to validate a NanoString nCounter ALK-fusion panel in routine biopsies of FFPE lung cancer patients. A total of 43 samples were analyzed, 13 ALK-positive and 30 ALK-negative, as previously detected by FISH and/or immunohistochemistry. Results: The NanoString panel detected the presence of the EML4-ALK, KIF5B-ALK and TFG-ALK fusion variants. We observed that all the 13 ALK-positive cases exhibited genetic aberrations by the NanoString methodology. Namely, six cases (46.15%) presented EML-ALK variant 1, two (15.38%) presented EML-ALK variant 2, two (15.38%) presented EML-ALK variant 3a, and three (23.07%) exhibited no variant but presented unbalanced expression between 5'/3' exons, similar to other positive samples. Importantly, for all these analyses, the initial input of RNA was 100 ng, and some cases displayed poor RNA quality measurements. Conclusions: In this study, we reported the great utility of NanoString technology in the assessment of ALK fusions in routine lung biopsies of FFPE specimens.This study was partially funded by FINEP (MCTI/FINEP/MS/SCTIE/DECIT), Brazil. BIOPLAT (1302/13).info:eu-repo/semantics/publishedVersio

IMPACTO DE VARIÁVEIS CONGNITIVAS E CONTEXTUAIS SOBRE O SUCESSO ACADÊMICO E O BEM ESTAR NA UNIVERSIDADE – QUE FAZER? QUE DEIXAR DE FAZER?

Este estudo parte de uma investigação longitudinal (2019-2022) mais ampla, vinculada ao Grupo Multidisciplinar de Pesquisa em Educação, Psicopedagogia e Psicologia Escolar, UFAM/CNPq, PROCAD/AMAZÔNIA-PPGEUFAM/UFPA/UFMT, Processo CAPES 8881.314288/2019-0, articula-se metodológica e teoricamente com interfaces entre a psicologia escolar, psicologia educacional, pedagogia do ensino superior e educação escolar. Atua no sentido de entender os efeitos de variáveis cognitivas e contextuais sobre o sucesso acadêmico e o bem estar na universidade. A metodologia para coleta de dados é a aplicação de caderno de instrumentos próprios com apoio do Googleodocs, a amostra atual é de n=1112 estudantes de diferentes países (Brasil, México, República Dominicana, Bolívia, Moçambique, Angola, Venezuela, Colômbia, Espanha e Portugal), do sexo masculino e feminino, idade entre 18 e 54 anos e de 51 universidades públicas e privadas que colaboram com a iniciativa. Os dados são analisados com auxílio do Excel e SPSS, de acordo com os interesses dos pesquisadores e objetivos da investigação. Os resultados atuais demonstram a importância da pesquisa sobre os temas abordados pela iniciativa considerando o impacto das variáveis estudadas sobre o rendimento acadêmico e bom estar tanto de estudantes como de docentes e técnicos no ensino superior. Foram identificadas diferenças de países, renda, etnia, gênero e renda. No caso dos docentes e técnicos verificamos evidências da exaustão feminina. Os resultados podem apoiar a administração geral e coordenação educativa e psicopedagógica universitária em sentido amplo, visando a melhoria dos indicadores de sucesso acadêmico e bem estar, por meio da proposição de novas políticas e gestão da educação superior. Palavras-chave: Pesquisa em educação, Ensino superior, Rendimento acadêmico, Bem estar. Políticas de gestão da educação superior. Educação comparada. &nbsp; IMPACT OF COGNITIVE AND CONTEXTUAL VARIABLES ABOUT ACADEMIC PERFORMANCE AND &nbsp;WELLBEING IN THE UNIVERSITY. Whato to do? Qhato not to do? &nbsp; Abstract: This study, part of a further longitudinal investigation (2019-2022), attached to a multidisciplinary group of Educational Research&nbsp; Scholar Psychotherapy and Psychology, UFAM/CNPq, PROCAD/AMAZONIA-PPGEUFAM/UFPA/UFMT, CAPES process 8881.314288/2019-0, it is articulated methodological and theoretically with interfaces between the scholar and educational psychology, upper and scholar education pedagogy. It acts in the direction of understanding 5he effects og cognitive and contextual variables about the academic achievement and wellness in the university. The methodology to data collection is the application of own instrument cards with the support of Googledocs, the actual sample is quantity of n=1112 students from 51 private and public universities of different countries (Brazil, Mexico, Dominican Republic, Bolivia, Mozambique, Angola, Venezuela, Colombia, Spain and Portugal), from both male and female genders, aging between 18 and 54 years-old. The data were analyzed with the support of software Excel and SPSS, according to the interests of researchers and investigation focus. The actual results show the importance of the research about the themes cited in the initiative considering the impacts of the studied variables over the academic performance and wellness of the students as the teachers and technicians from upper education. There were found differences between countries, income, ethnic and gender. In the cases of the teachers and technicians it was found evidences of female exhaustion. The results could support the general administration, educative and psychopedagogy coordination and in a wide range, looking forward the better successful academic indicators and wellness, through the proposition of new policies and management of upper education. Keywords: Educational Reserch, upper educatión, academic performance, wellness, upper educaciton mangement policies, compared education. &nbsp