Estudio del mecanismo de activación de la quinasa de proteínas dependiente de AMPc de Mucor Rouxii

Fil: Paveto, María Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Chagas' disease: an update on immune mechanisms and therapeutic strategies

The final decade of the 20th century was marked by an alarming resurgence in infectious diseases caused by tropical parasites belonging to the kinetoplastid protozoan order. Among the pathogenic trypanosomatids, some species are of particular interest due to their medical importance. These species include the agent responsible for Chagas' disease, Trypanosoma cruzi. Approximately 8 to 10 million people are infected in the Americas, and approximately 40 million are at risk. in the present review, we discuss in detail the immune mechanisms elicited during infection by T. cruzi and the effects of chemotherapy in controlling parasite proliferation and on the host immune system.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Nacional de Biologia Estrutural e QuImica Medicinal em Doencas Infecciosas (INBEQMeDI)Univ São Paulo, Inst Biomed Sci, Dept Parasitol, São Paulo, BrazilMackenzie Presbeterian Univ, Ctr Biomol Sci & Hlth, São Paulo, BrazilNatl Univ Rosario, Sch Med Sci, Inst Immunol, Rosario, Santa Fe, ArgentinaCSIC, Inst Parasitol & Biomed Lopez Neyra, Granada, SpainUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFAPESP: 08/57596-4FAPESP: 07/08648-9CNPq: 473906/2008-2Web of Scienc

Phytotoxic Effects of (±)-Catechin In vitro, in Soil, and in the Field

BACKGROUND: Exploring the residence time of allelochemicals released by plants into different soils, episodic exposure of plants to allelochemicals, and the effects of allelochemicals in the field has the potential to improve our understanding of interactions among plants. METHODOLOGY/PRINCIPAL FINDINGS: We conducted experiments in India and the USA to understand the dynamics of soil concentrations and phytotoxicity of (+/-)-catechin, an allelopathic compound exuded from the roots of Centaurea maculosa, to other plants in vitro and in soil. Experiments with single and pulsed applications into soil were conducted in the field. Experimental application of (+/-)-catechin to soils always resulted in concentrations that were far lower than the amounts added but within the range of reported natural soil concentrations. Pulses replenished (+/-)-catechin levels in soils, but consistently at concentrations much lower than were applied, and even pulsed concentrations declined rapidly. Different natural soils varied substantially in the retention of (+/-)-catechin after application but consistent rapid decreases in concentrations over time suggested that applied experimental concentrations may overestimate concentrations necessary for phytotoxicity by over an order of magnitude. (+/-)-Catechin was not phytotoxic to Bambusa arundinacea in natural Indian soil in a single pulse, but soil concentrations at the time of planting seeds were either undetectable or very low. However, a single dose of (+/-)-catechin suppressed the growth of bamboo in sand, in soil mixed with organic matter, and Koeleria macrantha in soils from Montana and Romania, and in field applications at 40 microg l(-1). Multiple pulses of (+/-)-catechin were inhibitory at very low concentrations in Indian soil. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that (+/-)-catechin is highly dynamic in natural soils, but is phytotoxic well below natural concentrations measured in some soils and applied at low concentrations in the field. However, there is substantial conditionality in the effects of the allelochemical

LA RICERCA DI UNA RAGIONEVOLE CEDEVOLEZZA DEL GIUDICATO

La tesi è incentrata sul valore del giudicato penale all'interno dell'ordinamento, mettendo in evidenza i punti critici che il suo principio di stabilità presenta rispetto alla tutela del diritto del condannato a subire una esecuzione che sia equa e giusta. Si argomenta inoltre l'iter percorso fino ad ora dalla giurisprudenza per mettere in luce la constatazione di una sempre minor resistenza del principio di intangibilità della sentenza divenuta irrevocabile

La Habana (Cuba). Aduanas (1799). 1:605

Comprende la parte de la ciudad de La Habana situada en torno a la aduana proyectada, desde el castillo de la Fuerza hasta la plaza de San Francisco y los muelles de la Caballería y de la Sal, además del plano de la planta del edificio proyectadoTítulo propio tomado del verso del documento, donde figuran además el autor y la fechaManuscrito firmado y rubricado por el autor. A plumilla en tinta negra y coloreado a la acuarela en varios coloresIndica los principales edificios de esta zona, así como las obras necesarias para la construcción de la aduana y su almacénFiguran diversas anotaciones y una cuadrícula a lápi

Estudio del mecanismo de activación de la quinasa de proteínas dependiente de AMPc de Mucor Rouxii

Fil: Paveto, María Cristina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Enzymatic and immunological detection of G protein α-subunits in the pathogenic fungus Candida albicans

AbstractGTP stimulation of adenylyl cyclase from the dimorphic pathogenic fungus Candida albicans is greatly enhanced by preincubation of membrane proteins with cholera toxin, NAD and ATP. In the presence of [32P]NAD the toxin catalyzes the covalent incorporation of radioactivity into a menbrane protein of 40 kDa. Pertussis toxin catalyzes the transference of the radioactivity from [32P]NAD to a 32 kDa protein. Two major proteins of 40–42 and 30–32 kDa can also be recognized in Western blots by an anti Gα-common antibody. The results support the idea that G proteins are part of the hormonesensory transduction chain of Candida [(1990) Biochem. Biophys. Res. Commun. 167. 1177-1183]

Matanzas (Cuba). Polvorines (1798). 1:214

Presenta la planta del almacén y del cuerpo de guardia y un perfil longitudinal y otro tansversal del primero de ellosManuscrito firmado y rubricado por el autor. Coloreado a la acuarela en varios coloresLeyenda explicativa en la que se señalan todos aquellos trabajos tenidos en cuenta para la elaboración del presente documento, así como la capacidad defensiva y de abastecimiento de este lugar en función del fuerte de San Severino y otros lugares adyacentes como el Morrillo y VigíaEn el ángulo superior derecho: "N°. 1°."Parece que deberían acompañar a este plano un croquis y un oficio con el presupuesto de la obra del almacén, tal y como se señala en la leyendaEn el verso figuran el título, autor, fecha y el nombre de Pavet