Effects of the Methanol Extract of Basella alba L (Basellaceae) on Steroid Production in Leydig Cells

In this study, Leydig cells were purified from 70 day-old Sprague Dawley male rats and incubated with 10 and 100 μg/mL of methanol extract of Basella alba (MEBa) for 4 hours followed by the evaluation of cell viability, steroid (testosterone and estradiol) production, and the level of aromatase mRNA. Results showed that MEBa did not affect Leydig cell viability. At the concentration of 10 μg/mL, MEBa significantly stimulated testosterone and estradiol production (p < 0.01 and p < 0.03, respectively), and enhanced aromatase mRNA level (p < 0.04). These observations suggest that MEBa directly stimulated testosterone, estradiol and aromatase mRNA levels in isolated Leydig cells

IN VITRO AMOEBICIDAL ACTIVITY OF SOME MEDICINAL PLANTS OF THE BAMUN REGION (CAMEROON)

Fifty two medicinal plants belonging to different families selected on the basis of their traditional use against jaundice and various liver disorders were tested for their amoebicidal activities. They were extracted and tested for their antiamoebic activity in vitro using polyxenic culture of Entamoebic histolytica. As the result, 14 exhibited an antiamoebic activity at a dose of 100µg/ml from the second to the fourth day of incubation. The 14 selected extracts were additionaly tested for 6 days at 10, 100 and 500g/ml of concentration, and only the leaves extract of Codiaeum variegatum exhibited a clear antiamoebic activity (EC50=10,74 the second day), and had a more pronounced activity than metronidazole the reference product

Effects of the aqueous extract of Pycnanthus angolensis (Myristicaceae) on male rat sexual behavior and nitric oxide release

Erectile dysfunction is a major public health problem leading to harmful consequences for couples. Hence the need to find adequate treatments using medicinal plants such as Pycnanthus angolensis, a Cameroonian plant used to treat erectile dysfunction. The effects of aqueous extract of Pycnanthus angolensis wood were evaluated on male rat sexual behavior and nitric oxide release. Adult male Wistar rats were divided into 4 groups of 5 each that included a group receiving orally: distilled water (10 ml/Kg BW), plant extract treatment (43 or 86 or 172 mg/kg BW). Sexual behaviors were monitored on days 1, 4, 7, and 14 by pairing male rats to receptive females and nitric oxide levels were assessed in penile homogenate. At the end of the 14 days of treatment, rats were killed and sexual organs weighed. No significant effect was observed on sexual organs weights. When administered at a dose of 43 mg/Kg, body weight; the plant extract significantly increased (p<0.05) the sexual performance as well as the libido of male rats. Nitric oxide release was significantly increased (p<0.05) in that group of extract-treated rats, while compared to the control group. The study demonstrates that Pycnanthus angolensis is a medicinal plant which can enhance the sexual behaviors of male rat. Hence, it can be used for the management of erectile dysfunction, mainly at a dose of 43 mg/Kg, body weight

Effect of Solubilizers on the Androgenic Activity of Basella Alba L. (Basellaceae) in Adult Male Rats

Purpose: Solubilizers play an important role in dissolution of pharmacological ingredients and should properly dissolve the active principle(s) while preserving its activities. This study investigated the effect of starch, gelatin, methylcellulose and polyvinylpyrrolidone 10000 in the preservation of the androgenic activity of the methanol extract Basella alba (MEBa). Methods: Different groups of male albino rats were orally given the MEBa (1 mg/kg) dissolved into either 1% gelatin (1% gel), %1 methylcellulose (1% MC) and 1% polyvinylpyrrolidone 10000 (1% PVP 10000) or 2% starch solution (2% SS) for 30 days. Thereafter, animals were sacrificed and serum testosterone and creatinine levels as well as alanine aminotransferase (ALT) activity determined. Vital and reproductive organs were dissected out and weighed, while liver thiobarbituric acid reactive substances (TBARS) and glutathione levels were determined. Results: Different treatments did not affect the animal body and organ weights. The MEBa stimulatory effect on testosterone production was preserved with 2% SS and 1% PVP 10000 as vehicles. Increased liver glutathione and TBARS levels were also observed in the animals fed with the MEBa dissolved in 2% SS and 1% Gel, respectively, while other biochemical parameters remained unchanged. Conclusion: Starch and polyvinylpyrrolidone 10000 stand as good preservation agents for MEBa androgenic activity, with starch exhibiting additional antioxidant activity through increase of glutathione levels

Inhibitory activity of limonoids from Khaya grandifoliola C.DC (Meliaceae) against hepatitis C virus infection in vitro

Objective: A fraction from Khaya grandifoliola has recently been shown to inhibit hepatitis C virus (HCV) infection and three limonoids (17-epi-methyl-6-hydroxylangolensate, 7-deacetoxy-7-oxogedunin and 7-deacetoxy-7R-hydroxygedunin) were purified from this fraction. The present study aimed at assessing the inhibitory effect of these limonoids on HCV using cell-culture derived HCV (HCVcc) system. Materials and Methods: Cytotoxic effects of the limonoids on Huh7.5 cells were assessed by MTT assay. Huh7.5 cells were transfected with RNA transcripts of the plasmid Jc1/GLuc2a, carrying a Gaussia luciferase reporter gene to rescue the HCVcc particles which were used to infect naïve cells in the presence or absence of the studied limonoids during 72 hr. Infection and replication rates were monitored by luciferase reporter assay and immunofluorescence assay (IFA) while cellular gene expression was analyzed by western blot, respectively. Results: The limonoids inhibited HCV infection mostly by targeting entry and replication stage. Their inhibitory effect on entry step, comparable to that of anti-CD81 antibody, was related to the blocking of CD81 receptor. In the replication step, the limonoids decreased the expression of NS5B similar to danoprevir. These compounds also significantly decreased but up-regulated the expression of Class-III phosphatidylinositol 4-kinase alpha and 2’,5’-oligoadenylate synthase-3, respectively. Conclusion: The present findings suggest that limonoids from K. grandifoliola are potential anti-HCV agents and may offer an advantage in the treatment of HCV infection

Studies on dyeing and bacterial resistance of Dacryodes macrophylla extract on woollen fabric

The dyeing of wool fabric using a natural dye extracted from Dacryodes macrophylla has been studied using both conventional as well as ultrasonic dyeing methods. Natural dye was extracted using water as solvent by Soxhlet extraction method. The extracted color was characterized by spectroscopic studies like UV/Visible and further tested for antimicrobial activity (against Vibrio cholerae (MTCC3906), Salmonella enterica serotype typhi (MTCC733), Shigella flexneri (MTCC1457), Escherichia coli (MTCC119), Listeria monocytogenes (MTCC657) and Staphylococcus aureus (MTCC 96). It was observed that the water extract of D. macrophylla showed antimicrobial activities against S. enterica. The effect of mordants on the dyeing outcome in terms of colour depth as well as fastness properties of the dyeing was investigated.&nbsp; It has been found that employing all the three techniques of dyeing i.e., conventional dyeing at 100 oC and 80 oC as well as ultrasonicated dyeings conducted at 80 oC, the mordanted samples were deeper in depth than their unmordanted counterparts. The results of fastness properties of the dyed fabrics were fair to good. Depths of the shade and fastness properties in case of ultrasonic dyeing were found to be better compared to conventional dyeing. Dacryodes macrophyllaextract&nbsp; was found to enhance microbial resistance of wool as well as was itself as a good source of coloration. These textiles dyed with the natural dyes can be very useful in developing clothing for infants, elderly and infirm people to protect them against common infections

Antihepatotoxic and Antioxidant Activities of Methanol Extract and Isolated Compounds from Ficus chlamydocarpa

Free radicals, in particular radical oxygen species (ROS), play an important role in the aetiology and pathogenesis of various diseases. Current research in many countries focuses on the use of local medicinal plants as a promising source of liver protective agents. This paper describes the hepatoprotective effects of the methanol extract and four isolated compounds from Ficus chlamydocarpa on CCl4-induced liver damage, as well as the possible antioxidant mechanisms involved in this protection. The DPPH test, along with the ß-Carotene-Linoleic Acid Model System and Ferric-Reducing Antioxidant Power assays, as well as the inhibition of microsomal lipid peroxidation were used to measure radical-scavenging and antioxidant activities. Pretreatment of rats with the methanol extract of F. chlamydocarpa before CCl4administration, significantly prevented serum increase of hepatic enzyme markers, glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT), in a dose-dependent manner. The hepatoprotection was also associated with a significant enhancement in hepatic reduced glutathione (GSH) and a marked decrease of liver malondialdehyde (MDA). Among the four compounds 1-4, isolated from the methanol extract, α-amyrin acetate (1) and luteolin (4) showed a significant hepatoprotective activity, as indicated by their ability to prevent liver cell death and lactate dehydrogenase (LDH) leakage during CCl4intoxication

Aflatoxin Contamination in Food and Body Fluids in Relation to Malnutrition and Cancer Status in Cameroon

Aflatoxins are food contaminants usually associated with hepatitis, immunodepression, impairment of fertility and cancer. The present work was to determine the presence of aflatoxins in eggs, milk, urine, and blood samples that were collected from various sources and periods; and hepatitis B virus antigen in blood samples. Aflatoxin was found in eggs (45.2%), cow raw milk (15.9%), breast milk (4.8%), urine from kwashiorkor and marasmic kwashiorkor children (45.5%), and sera from primary liver cancer patients (63.9%); HbsAg was also detected in 69.4% of the serum samples, but there was no association between both factors. Both AF and hepatitis B virus seem to be risk factors that could increase the incidence and prevalence rates of malnutrition and cancer in Cameroon

Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity

Drug-induced liver injury (DILI) is a major clinical problem where natural compounds hold promise for its abrogation. Khaya grandifoliola (Meliaceae) is used in Cameroonian traditional medicine for the treatment of liver related diseases and has been studied for its hepatoprotective properties. Till date, reports showing the hepatoprotective molecular mechanism of the plant are lacking. The aim of this study was therefore to identify compounds from the plant bearing hepatoprotective activity and the related molecular mechanism by assessing their effects against acetaminophen (APAP)-induced hepatotoxicity in normal human liver L-02 cells line. The cells were exposed to APAP (10 mM) or co-treated with phytochemical compounds (40 μM) over a period of 36 h and, biochemical and molecular parameters assessed. Three known limonoids namely 17-epi-methyl-6-hydroxylangolensate, 7-deacetoxy-7-oxogedunin and deacetoxy-7R-hydroxygedunin were identified. The results of cells viability and membrane integrity, reactive oxygen species generation and lipid membrane peroxidation assays, cellular glutathione content determination as well as expression of cytochrome P450 2E1 demonstrated the protective action of the limonoids. Immunoblotting analysis revealed that limonoids inhibited APAP-induced c-Jun N-terminal Kinase phosphorylation (p-JNK), mitochondrial translocation of p-JNK and Bcl2-associated X Protein, and the release of Apoptosis-inducing Factor into the cytosol. Interestingly, limonoids increased the expression of Mitogen-activated Protein Kinase Phosphatase (Mkp)-1, an endogenous inhibitor of JNK phosphorylation and, induced the nuclear translocation of Nuclear Factor Erythroid 2-related Factor-2 (Nrf2) and decreased the expression of Kelch-like ECH-associated Protein-1. The limonoids also reversed the APAP-induced decreased mRNA levels of Catalase, Superoxide Dismutase-1, Glutathione-S-Transferase and Methionine Adenosyltransferase-1A. The obtained results suggest that the isolated limonoids protect L-02 hepatocytes against APAP-induced hepatotoxicity mainly through increase expression of Mkp-1 and nuclear translocation of Nrf2. Thus, these compounds are in part responsible of the hepatoprotective activity of K. grandifoliola and further analysis including in vivo and toxicological studies are needed to select the most potent compound that may be useful as therapeutic agents against DILI