Production of high levels of poly-3-hydroxybutyrate in plastids of Camelina sativa seeds

Poly-3-hydroxybutyrate (PHB) production in plastids of Camelina sativa seeds was investigated by comparing levels of polymer produced upon transformation of plants with five different binary vectors containing combinations of five seed-specific promoters for expression of transgenes. Genes encoding PHB biosynthetic enzymes were modified at the N-terminus to encode a plastid targeting signal. PHB levels of up to 15% of the mature seed weight were measured in single sacrificed T1 seeds with a genetic construct containing the oleosin and glycinin promoters. A more detailed analysis of the PHB production potential of two of the best performing binary vectors in a Camelina line bred for larger seed size yielded lines containing up to 15% polymer in mature T2 seeds. Transmission electron microscopy showed the presence of distinct granules of PHB in the seeds. PHB production had varying effects on germination, emergence and survival of seedlings. Once true leaves formed, plants grew normally and were able to set seeds. PHB synthesis lowered the total oil but not the protein content of engineered seeds. A change in the oil fatty acid profile was also observed. High molecular weight polymer was produced with weight-averaged molecular weights varying between 600 000 and 1 500 000, depending on the line. Select lines were advanced to later generations yielding a line with 13.7% PHB in T4 seeds. The levels of polymer produced in this study are the highest reported to date in a seed and are an important step forward for commercializing an oilseed-based platform for PHB production

Incorporating temporal-bounded CBR techniques in real-time agents

Nowadays, MAS paradigm tries to move Computation to a new level of abstraction: Computation as interaction, where large complex systems are seen in terms of the services they offer, and consequently in terms of the entities or agents providing or consuming services. However, MAS technology is found to be lacking in some critical environments as real-time environments. An interaction-based vision of a real-time system involves the purchase of a responsibility by any entity or agent for the accomplishment of a required service under possibly hard or soft temporal conditions. This vision notably increases the complexity of these kinds of systems. The main problem in the architecture development of agents in real-time environments is with the deliberation process where it is difficult to integrate complex bounded deliberative processes for decision-making in a simple and efficient way. According to this, this work presents a temporal-bounded deliberative case-based behaviour as an anytime solution. More specifically, the work proposes a new temporal-bounded CBR algorithm which facilitates deliberative processes for agents in real-time environments, which need both real-time and deliberative capabilities. The paper presents too an application example for the automated management simulation of internal and external mail in a department plant. This example has allowed to evaluate the proposal investigating the performance of the system and the temporal-bounded deliberative case-based behaviour. 2010 Elsevier Ltd. All rights reserved.This work is supported by TIN2006-14630-C03-01 projects of the Spanish government, GVPRE/2008/070 project, FEDER funds and CONSOLIDER-INGENIO 2010 under Grant CSD2007-00022.Navarro Llácer, M.; Heras Barberá, SM.; Julian Inglada, VJ.; Botti Navarro, VJ. (2011). Incorporating temporal-bounded CBR techniques in real-time agents. Expert Systems with Applications. 38(3):2783-2796. https://doi.org/10.1016/j.eswa.2010.08.070S2783279638

A study of the properties of Leptosphaeria maculans pathotypes by restriction fragment length polymorphism and expression of stress-responsive genes, and defense gene expression in Brassica napus

Bibliography: p. 266-300

Saponin Biosynthesis in Pulses

Pulses are a group of leguminous crops that are harvested solely for their dry seeds. As the demand for plant-based proteins grows, pulses are becoming important food crops worldwide. In addition to being a rich source of nutrients, pulses also contain saponins that are traditionally considered anti-nutrients, and impart bitterness and astringency. Saponins are plant secondary metabolites with great structural and functional diversity. Given their diverse functional properties and biological activities, both undesirable and beneficial, saponins have received growing attention. It can be expected that redirecting metabolic fluxes to control the saponin levels and produce desired saponins would be an effective approach to improve the nutritional and sensory quality of the pulses. However, little effort has been made toward understanding saponin biosynthesis in pulses, and, thus there exist sizable knowledge gaps regarding its pathway and regulatory network. In this paper, we summarize the research progress made on saponin biosynthesis in pulses. Additionally, phylogenetic relationships of putative biosynthetic enzymes among multiple pulse species provide a glimpse of the evolutionary routes and functional diversification of saponin biosynthetic enzymes. The review will help us to advance our understanding of saponin biosynthesis and aid in the development of molecular and biotechnological tools for the systematic optimization of metabolic fluxes, in order to produce the desired saponins in pulses

In silico modeling of FDA-approved drugs for discovery of therapies against neglected diseases: A drug repurposing approach

Drug repurposing (i.e., finding new therapeutic uses for already known drugs, including approved, abandoned, discontinued, and investigational drugs) is being extensively applied worldwide to expedite the development of novel therapeutic solutions. Such strategy could be particularly relevant in the fields of neglected and rare diseases, where drug development funding is scarce and most of the ongoing research is funded by the public sector or not-for-profit initiatives. In silico tools can make the drug repurposing approach even more cost-efficient. Here, we present an overview of cheminformatic, bioinformatic, text mining, and network-based approaches that are currently applied to guide drug repurposing, including virtual screening, target fishing, estimation of molecular determinants of promiscuity, binding site similarity predictions, the similarity ensemble approach, among others. Specific applications of such techniques within the field of neglected conditions are discussed, when available.Fil: Bellera, Carolina Leticia. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Sbaraglini, Maria Laura. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Alberca, Lucas Nicolás. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Alice, Juan Ignacio. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Talevi, Alan. Universidad Nacional de La Plata. Facultad de Ciencas Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin