Adapted Pool Cue

Our primary goal was to develop an adaptive device to allow a user with limited upper body mobility to more effectively play a game of pool. Within this goal it was important to design around the concept of a least restrictive environment, in order to provide the user with as close to a standard pool playing experience as possible. The device was designed to specifically meet the needs of John Lee, an Assistive Technology Specialist at Cal Poly\u27s Disability Resource Center. Mr. Lee has muscular dystrophy, which limits his upper and lower body mobility and currently requires his use of a wheelchair. Research into the specific needs of our customer, current products, and pool game elements was performed to better understanding of the project goals. From this research and discussion with John Lee, customer requirements and engineering specifications were developed. These were then used to guide the design development process. Several ideation techniques were used, and concepts for a bridge mechanism and supporting bases were created. A rough prototype was developed to test the concepts before our final design direction was decided. Our final design acts as a robust bridge device with a self-supporting base that constrains the path of the pool cue to a set path, allowing the user to focus their effort on providing power to the shot. The base can be rolled around the table, and stabilizing posts are lowered through a cable system when the shot is being made to prevent the base from sliding. The shooting mechanism features horizontal and vertical angle adjustment to allow for proper positioning of the cue, as well as a small cue stop to adjust the allowable travel of the pool cue. A vertical height adjustment is also present to allow the mechanism to reach over the edge of the pool table and for special high angle shots. The shooting mechanism itself features fixed and sliding rollers that act as a modified pool bridge, limiting travel of the pool cue to one axis of motion. The top part of the device can be separated from the base and clamped to the upright post for easier transport. These design choices allow the device to be compatible with any size regulation pool cue. Engineering analysis was performed to select materials and size components for the final device. Parts were specified and costs recorded to keep within the program budget. The device was then manufactured to the specifications shown in the included technical drawings, and testing was performed to verify that the final product met the design requirements. The overall experience was successful, and the final product received strong approval from John Lee. Recommendations for potential future improvement of both manufacturing and design of the device are included, as are as tips for device operation

DNA Methylation: Bisulphite Modification and Analysis

Epigenetics describes the heritable changes in gene function that occur independently to the DNA sequence. The molecular basis of epigenetic gene regulation is complex, but essentially involves modifications to the DNA itself or the proteins with which DNA associates. The predominant epigenetic modification of DNA in mammalian genomes is methylation of cytosine nucleotides (5-MeC). DNA methylation provides instruction to gene expression machinery as to where and when the gene should be expressed. The primary target sequence for DNA methylation in mammals is 5'-CpG-3' dinucleotides (Figure 1). CpG dinucleotides are not uniformly distributed throughout the genome, but are concentrated in regions of repetitive genomic sequences and CpG "islands" commonly associated with gene promoters (Figure 1). DNA methylation patterns are established early in development, modulated during tissue specific differentiation and disrupted in many disease states including cancer. To understand the biological role of DNA methylation and its role in human disease, precise, efficient and reproducible methods are required to detect and quantify individual 5-MeCs

An Open-Source Plate Reader

Microplate readers are foundational instruments in experimental biology and bioengineering that enable multiplexed spectrophotometric measurements. To enhance their accessibility, we here report the design, construction, validation, and benchmarking of an open-source microplate reader. The system features full-spectrum absorbance and fluorescence emission detection, in situ optogenetic stimulation, and stand-alone touch screen programming of automated assay protocols. The total system cost

COVID-19 and Power in Global Health

Political scientists bring important tools to the analysis of the coronavirus disease 2019 (COVID-19) pandemic, particularly a focus on the crucial role of power in global health politics. We delineate different kinds of power at play during the COVID-19 crisis, showing how a dearth of compulsory, institutional, and epistemic power undermined global cooperation and fueled the pandemic, with its significant loss to human life and huge economic toll. Through the pandemic response, productive and structural power became apparent, as issue frames stressing security and then preserving livelihoods overwhelmed public health and human rights considerations. Structural power rooted in economic inequalities between and within countries conditioned responses and shaped vulnerabilities, as the crisis threatened to deepen power imbalances along multiple lines. Calls for global health security will surely take on a new urgency in the aftermath of the pandemic and the forms of power delineated here will shape their outcome

A Fully Nonmetallic Gas Turbine Engine Enabled by Additive Manufacturing of Ceramic Composites

This publication is the third part of a three part report of the project entitled "A Fully Nonmetallic Gas Turbine Engine Enabled by Additive Manufacturing" funded by NASA Aeronautics Research Institute (NARI). The objective of this project was to conduct additive manufacturing to produce ceramic matrix composite materials and aircraft engine components by the binder jet process. Different SiC powders with median sizes ranging from 9.3 to 53.0 microns were investigated solely and in powder blends in order to maximize powder packing. Various infiltration approaches were investigated to include polycarbosilane (SMP-10), phenolic, and liquid silicon. Single infiltrations of SMP-10 and phenolic only slightly filled in the interior. When the SMP-10 was loaded with sub-micron sized SiC powders, the infiltrant gave a much better result of filling in the interior. Silicon carbide fibers were added to the powder bed to make ceramic matrix composite materials. Microscopy showed that the fibers were well distributed with no preferred orientation on the horizontal plane and fibers in the vertical plane were at angles as much as 45deg. Secondary infiltration steps were necessary to further densify the material. Two to three extra infiltration steps of SMP-10 increased the density by 0.20 to 0.55 g/cc. However, the highest densities achieved were 2.10 to 2.15 g/cc. Mechanical tests consisting of 4 point bend tests were conducted. Samples from the two CMC panels had higher strengths and strains to failure than the samples from the two nonfiber reinforced panels. The highest strengths were from Set N with 65 vol% fiber loading which had an average strength of 66 MPa. Analysis of the fracture surfaces did not reveal pullout of the reinforcing fibers. Blunt fiber failure suggested that there was not composite behavior. The binder jet additive manufacturing method was used to also demonstrate the fabrication of turbine engine vane components of two different designs and sizes. The binder jet method has benefits over the conventional manufacturing of CMCs in that prototype and production parts can be fabricated quickly and economically with no tooling and extensive hand layup

Teaching for Black and Brown Lives: The Importance of Ethnic Studies Curriculum in the Education Success of Black and Latinx Students

This study identifies school policies and practices that create a gap in support for Ethnic Studies for high school graduation, college graduation, and teacher licensures. The first method was interviewing specific stakeholders through zoom who were in the position for the purpose of the research. The interviewed participants answered questions about the policies related to Ethnic studies, practice school policies, and implications of the ratification of Ethnic study policies in Nevada. The second method was analyzing Nevada state and Department of Education policies relating to teacher education. Interpreting how UNLV teacher licensure programs apply those policies in the preparation curriculum. Results from the methods were initial findings were 75% of the student population in CCSD are students of color, but there are still limited clubs that surround racialized experiences. Although students who participated in racial/ethnic club activities led to more enrollment in elective courses. Ethnic studies are considered along with social studies and “diversity studies”, which need to be differentiated from one another. Ethnic study courses can be a valuable enhancement to build cross-racial solidarity and create opportunities to make sense of school experiences. It is critical to make a foundation for success for Students of Color and Families of color in many schools and communities in CCSD.https://digitalscholarship.unlv.edu/durep_lightning/1003/thumbnail.jp

Pulsed extraction of ionization from helium buffer gas

The migration of intense ionization created in helium buffer gas under the influence of applied electric fields is considered. First the chemical evolution of the ionization created by fast heavy-ion beams is described. Straight forward estimates of the lifetimes for charge exchange indicate a clear suppression of charge exchange during ion migration in low pressure helium. Then self-consistent calculations of the migration of the ions in the electric field of a gas-filled cell at the National Superconducting Cyclotron Laboratory (NSCL) using a Particle-In-Cell computer code are presented. The results of the calculations are compared to measurements of the extracted ion current caused by beam pulses injected into the NSCL gas cell.Comment: Accepted for pubilication in Nucl. Instrum. Meth. B, 14 pages, 8 figure

Holocene fire regimes and treeline migration rates in sub-arctic Canada

Holocene climate change resulted in major vegetation reorganization in sub-arctic Canada near modern treeline. However, little is known of the effects of long-term climate change on boreal forest composition and fire regimes below treeline in this region. We present a high-resolution vegetation and fire history from two sites within the modern boreal forest in the central Northwest Territories, Canada, to provide new insight on sub-arctic vegetation response to Holocene climate dynamics and the role of fire in boreal ecosystems. Palynological analysis of sediments retrieved from Waite and Danny's lakes (informal) is used to reconstruct regional vegetation dynamics and boreal fire regimes. The longer Danny's Lake record documents treeline expansion beginning at ca. 7430–7220 cal yr BP. Integration of our new data with previous work shows that treeline expanded between ca. 4050 cal. yr BP and ca. 3840 cal yr BP at a rate of ca. 50 m/yr in response to the 1–2 °C increase in temperature estimated for the Holocene Thermal Maximum. Forest fires were relatively frequent during the early Holocene, before declining in frequency in response to development of cooler and wetter climate conditions associated with the Neoglacial (beginning after ca. 2200–2320 cal yr BP). We document a trend of increasing fire frequency in the 20th century that is correlated with warming at this time. These dynamics south of modern treeline provide insight into factors creating heterogeneity in plant community responses to large-scale climate events in high northern latitudes and suggest that large scale reorganization of boreal vegetation and fire regimes can be expected over the coming decades

Implementing outside the box: Community-based social service provider experiences with using an alcohol screening and intervention

Objective: The aim of this study is better understand perceptions of front-line social service workers who are not addiction specialists, but have to address addiction-related issues during their standard services. Method: Six social service organizations implemented a validated alcohol assessment and brief education intervention. After a 3-month trial implementation period, a convenience sample of 64 front-line providers participated in six focus groups to examine barriers and facilitators to the implementation of an alcohol screening and brief intervention. Results: Three themes emerged: (1) usefulness of the intervention, (2) intervention being an appropriate fit with the agency and client population, and (3) worker commitment and proper utilization during the implementation process. Conclusions: A cross-cutting theme that emerged was the context in which the intervention was implemented, as this was central to each of the three primary themes identified from the focus groups (i.e., the usefulness and appropriateness of the intervention and the implementation process overall). Practitioner buy-in concerns also indicate the need for better addiction service training opportunities for those without addiction-specific educational backgrounds. Future research should assess whether targeted trainings increase addiction screening and education in social services settings