Evaluation of the Interaction Between TGF β and Nitric Oxide in the Mechanisms of Progression of Colon Carcinoma

It is recognised that stromal cells determine cancer progression. We have previously shown that active TGFβ produced by rat colon carcinoma cells modulated NO production in rat endothelial cells. To elucidate the role of TGFβ and NO in the mechanisms of interaction of colon carcinoma cells with stromal cells and in cancer progression, we transfected REGb cells, a regressive colon carcinoma clone secreting latent TGFβ, with a cDNA encoding for a constitutively-secreted active TGFβ. Out of 20 injected rats only one tumour progressed, which was resected and sub-cultured (ReBeta cells). ReBeta cells secreted high levels of active TGFβ. The adhesive properties of REGb and Rebeta cells to endothelial cells were similar, showing that the secretion of active TGFβ is not involved in tumour cell adhesion to endothelial cells. ReBeta, but not REGb, cell culture supernatants inhibited cytokine-dependent NO secretion by endothelial cells, but inhibition of NO production was similar in co-cultures of REGb or ReBeta cells with endothelial cells. Therefore, secretion of active TGFβ regulated endothelial NO synthase activity when tumour cells were distant from, but not in direct contact with, endothelial cells. However, only ReBeta cells inhibited cytokine-dependent secretion of NO in coculture with macrophages, indicating that the active-TGFβ-NO axis confers an advantage for tumour cells in their interaction with macrophages rather than endothelial cells in cancer progressio

Dust mass-loss rates from AGB stars in the Fornax and Sagittarius dwarf Spheroidal galaxies

To study the effect of metallicity on the mass-loss rate of asymptotic giant branch (AGB) stars, we have conducted mid-infrared photometric measurements of such stars in the Sagittarius (Sgr dSph) and Fornax dwarf spheroidal galaxies with the 10-$\mu$m camera VISIR at the VLT. We derive mass-loss rates for 29 AGB stars in Sgr dSph and 2 in Fornax. The dust mass-loss rates are estimated from the $K-[9]$ and $K-[11]$ colours. Radiative transfer models are used to check the consistency of the method. Published IRAS and Spitzer data confirm that the same tight correlation between $K-[12]$ colour and dust mass-loss rates is observed for AGB stars from galaxies with different metallicities, i.e. the Galaxy, the LMC and the SMC. The derived dust mass-loss rates are in the range 5$\times10^{-10}$ to 3$\times10^{-8}$ M$_{\odot}$yr$^{-1}$ for the observed AGB stars in Sgr dSph and around 5$\times10^{-9}$ M$_{\odot}$yr$^{-1}$ for those in Fornax; while values obtained with the two different methods are of the same order of magnitude. The mass-loss rates for these stars are higher than the nuclear burning rates, so they will terminate their AGB phase by the depletion of their stellar mantles before their core can grow significantly. Some observed stars have lower mass-loss rates than the minimum value predicted by theoretical models.Comment: 12 pages, 9 figures, accepted for publication in MNRA

Asymptotic Giant Branch Stars in the Leo I Dwarf Spheroidal Galaxy

Twenty six Asymptotic Giant Branch (AGB) variables are identified in the Local Group galaxy Leo I. These include 7 Mira and 5 semi-regular variables for which periods, amplitudes and mean magnitudes are determined. The large range of periods for the Miras, 158<P<523 days, suggests an AGB spanning a significant age range. The youngest must be around 1.6 Gyr while the oldest could be 10 Gyr or more. Two of these old Miras are found in the outer regions of Leo I (over 490 arcsec from the centre) where stars on the extended AGB are rare. They could provide an interesting test of third dredge-up and mass loss in old stars with low metallicity and are worth further detailed investigation. At least two stars, one a Mira, the other an irregular variable, are undergoing obscuration events due to dust ejection. An application of the Mira period-luminosity relation to these stars yields a distance modulus for Leo I of (m-M)=21.80 \pm 0.11 mag (internal), \pm 0.12 (total) (on a scale that puts the LMC at 18.39 mag) in good agreement with other determinations.Comment: 10 pages, 8 figures, accepted for publication in MNRAS

AGB Stars in the Fornax Dwarf Spheroidal Galaxy

We report on a multi-epoch study of the Fornax dwarf spheroidal galaxy, made with the Infrared Survey Facility, over an area of about 42'x42'. The colour-magnitude diagram shows a broad well-populated giant branch with a tip that slopes down-wards from red to blue, as might be expected given Fornax's known range of age and metallicity. The extensive AGB includes seven Mira variables and ten periodic semi-regular variables. Five of the seven Miras are known to be carbon rich. Their pulsation periods range from 215 to 470 days, indicating a range of initial masses. Three of the Fornax Miras are redder than typical LMC Miras of similar period, probably indicating particularly heavy mass-loss rates. Many, but not all, of the characteristics of the AGB are reproduced by isochrones from Marigo et al. for a 2 Gyr population with a metallicity of Z=0.0025. An application of the Mira period-luminosity relation to these stars yields a distance modulus for Fornax of 20.69+/-0.04 (internal), +/-0.08 (total) (on a scale that puts the LMC at 18.39 mag) in good agreement with other determinations. Various estimates of the distance to Fornax are reviewed.Comment: 15 pages, 13 figures, accepted for publication in MNRA

Interaction of Saccharomyces boulardii with Salmonella enterica Serovar Typhimurium Protects Mice and Modifies T84 Cell Response to the Infection

BACKGROUND: Salmonella pathogenesis engages host cells in two-way biochemical interactions: phagocytosis of bacteria by recruitment of cellular small GTP-binding proteins induced by the bacteria, and by triggering a pro-inflammatory response through activation of MAPKs and nuclear translocation of NF-kappaB. Worldwide interest in the use of functional foods containing probiotic bacteria for health promotion and disease prevention has increased significantly. Saccharomyces boulardii is a non-pathogenic yeast used as a probiotic in infectious diarrhea. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we reported that S. boulardii (Sb) protected mice from Salmonella enterica serovar Typhimurium (ST)-induced death and prevented bacterial translocation to the liver. At a molecular level, using T84 human colorectal cancer cells, we demonstrate that incubation with Sb before infection totally abolished Salmonella invasion. This correlates with a decrease of activation of Rac1. Sb preserved T84 barrier function and decreased ST-induced IL-8 synthesis. This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2, p38 and JNK as well as on activation of NF-kappaB. Electron and confocal microscopy experiments showed an adhesion of bacteria to yeast cells, which could represent one of the mechanisms by which Sb exerts its protective effects. CONCLUSIONS: Sb shows modulating effects on permeability, inflammation, and signal transduction pathway in T84 cells infected by ST and an in vivo protective effect against ST infection. The present results also demonstrate that Sb modifies invasive properties of Salmonella

EuReCa ONE—27 Nations, ONE Europe, ONE Registry A prospective one month analysis of out-of-hospital cardiac arrest outcomes in 27 countries in Europe

AbstractIntroductionThe aim of the EuReCa ONE study was to determine the incidence, process, and outcome for out of hospital cardiac arrest (OHCA) throughout Europe.MethodsThis was an international, prospective, multi-centre one-month study. Patients who suffered an OHCA during October 2014 who were attended and/or treated by an Emergency Medical Service (EMS) were eligible for inclusion in the study. Data were extracted from national, regional or local registries.ResultsData on 10,682 confirmed OHCAs from 248 regions in 27 countries, covering an estimated population of 174 million. In 7146 (66%) cases, CPR was started by a bystander or by the EMS. The incidence of CPR attempts ranged from 19.0 to 104.0 per 100,000 population per year. 1735 had ROSC on arrival at hospital (25.2%), Overall, 662/6414 (10.3%) in all cases with CPR attempted survived for at least 30 days or to hospital discharge.ConclusionThe results of EuReCa ONE highlight that OHCA is still a major public health problem accounting for a substantial number of deaths in Europe.EuReCa ONE very clearly demonstrates marked differences in the processes for data collection and reported outcomes following OHCA all over Europe. Using these data and analyses, different countries, regions, systems, and concepts can benchmark themselves and may learn from each other to further improve survival following one of our major health care events

Mise au point d'un traitement par des endotoxines de carcinomatoses peritoneales induites par des cellules cancereuses coliques chez le rat et etude des mecanismes

SIGLECNRS T Bordereau / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

Lack of effect of a high polyunsaturated fat diet on the growth of transplantable colon tumors and on the cytolytic activity of macrophages in rats

International audienc

Understanding the Progression of Bone Metastases to Identify Novel Therapeutic Targets

Bone is one of the most preferential target site for cancer metastases, particularly for prostate, breast, kidney, lung and thyroid primary tumours. Indeed, numerous chemical signals and growth factors produced by the bone microenvironment constitute factors promoting cancer cell invasion and aggression. After reviewing the different theories proposed to provide mechanism for metastatic progression, we report on the gene expression profile of bone-seeking cancer cells. We also discuss the cross-talk between the bone microenvironment and invading cells, which impacts on the tumour actions on surrounding bone tissue. Lastly, we detail therapies for bone metastases. Due to poor prognosis for patients, the strategies mainly aim at reducing the impact of skeletal-related events on patients’ quality of life. However, recent advances have led to a better understanding of molecular mechanisms underlying bone metastases progression, and therefore of novel therapeutic targets

LOCODOX: a Software for Argo Oxygen data correction

&lt;p&gt;A few modifications from C. Kermabon and T. Reynaud&lt;/p&gt;If you use this software, please cite it as below