Seismology of Procyon A: determination of mode frequencies, amplitudes, lifetimes, and granulation noise

The F5 IV-V star Procyon A (aCMi) was observed in January 2001 by means of the high resolution spectrograph SARG operating with the TNG 3.5m Italian telescope (Telescopio Nazionale Galileo) at Canary Islands, exploiting the iodine cell technique. The time-series of about 950 spectra carried out during 6 observation nights and a preliminary data analysis were presented in Claudi et al. 2005. These measurements showed a significant excess of power between 0.5 and 1.5 mHz, with ~ 1 m/s peak amplitude. Here we present a more detailed analysis of the time-series, based on both radial velocity and line equivalent width analyses. From the power spectrum we found a typical p-mode frequency comb-like structure, identified with a good margin of certainty 11 frequencies in the interval 0.5-1400 mHz of modes with l=0,1,2 and 7< n < 22, and determined large and small frequency separations, Dn = 55.90 \pm 0.08 muHz and dnu_02=7.1 \pm 1.3 muHz, respectively. The mean amplitude per mode (l=0,1) at peak power results to be 0.45 \pm 0.07 m/s, twice larger than the solar one, and the mode lifetime 2 \pm 0.4 d, that indicates a non-coherent, stochastic source of mode excitation. Line equivalent width measurements do not show a significant excess of power in the examined spectral region but allowed us to infer an upper limit to the granulation noise.Comment: 10 pages, 15 figures, 4 tables. Accepted for publication in A&

Internal rotation of the red-giant star KIC 4448777 by means of asteroseismic inversion

In this paper we study the dynamics of the stellar interior of the early red-giant star KIC 4448777 by asteroseismic inversion of 14 splittings of the dipole mixed modes obtained from {\it Kepler} observations. In order to overcome the complexity of the oscillation pattern typical of red-giant stars, we present a procedure which involves a combination of different methods to extract the rotational splittings from the power spectrum. We find not only that the core rotates faster than the surface, confirming previous inversion results generated for other red giants (Deheuvels et al. 2012,2014), but we also estimate the variation of the angular velocity within the helium core with a spatial resolution of $\Delta r=0.001R$ and verify the hypothesis of a sharp discontinuity in the inner stellar rotation (Deheuvels et al. 2014). The results show that the entire core rotates rigidly with an angular velocity of about $\langle\Omega_c/2\pi\rangle=748\pm18$~nHz and provide evidence for an angular velocity decrease through a region between the helium core and part of the hydrogen burning shell; however we do not succeed to characterize the rotational slope, due to the intrinsic limits of the applied techniques. The angular velocity, from the edge of the core and through the hydrogen burning shell, appears to decrease with increasing distance from the center, reaching an average value in the convective envelope of $\langle\Omega_s/2\pi\rangle=68\pm22$~nHz. Hence, the core in KIC~4448777 is rotating from a minimum of 8 to a maximum of 17 times faster than the envelope. We conclude that a set of data which includes only dipolar modes is sufficient to infer quite accurately the rotation of a red giant not only in the dense core but also, with a lower level of confidence, in part of the radiative region and in the convective envelope.Comment: accepted for publication on Ap

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador

Background: Rhodnius ecuadoriensis is the main triatomine vector of Chagas disease, American trypanosomiasis, in Southern Ecuador and Northern Peru. Genomic approaches and next generation sequencing technologies have become powerful tools for investigating population diversity and structure which is a key consideration for vector control. Here we assess the effectiveness of three different 2b restriction site-associated DNA (2b-RAD) genotyping strategies in R. ecuadoriensis to provide sufficient genomic resolution to tease apart microevolutionary processes and undertake some pilot population genomic analyses. Methodology/Principal findings: The 2b-RAD protocol was carried out in-house at a non-specialized laboratory using 20 R. ecuadoriensis adults collected from the central coast and southern Andean region of Ecuador, from June 2006 to July 2013. 2b-RAD sequencing data was performed on an Illumina MiSeq instrument and analyzed with the STACKS de novo pipeline for loci assembly and Single Nucleotide Polymorphism (SNP) discovery. Preliminary population genomic analyses (global AMOVA and Bayesian clustering) were implemented. Our results showed that the 2b-RAD genotyping protocol is effective for R. ecuadoriensis and likely for other triatomine species. However, only BcgI and CspCI restriction enzymes provided a number of markers suitable for population genomic analysis at the read depth we generated. Our preliminary genomic analyses detected a signal of genetic structuring across the study area. Conclusions/Significance: Our findings suggest that 2b-RAD genotyping is both a cost effective and methodologically simple approach for generating high resolution genomic data for Chagas disease vectors with the power to distinguish between different vector populations at epidemiologically relevant scales. As such, 2b-RAD represents a powerful tool in the hands of medical entomologists with limited access to specialized molecular biological equipment. Author summary: Understanding Chagas disease vector (triatomine) population dispersal is key for the design of control measures tailored for the epidemiological situation of a particular region. In Ecuador, Rhodnius ecuadoriensis is a cause of concern for Chagas disease transmission, since it is widely distributed from the central coast to southern Ecuador. Here, a genome-wide sequencing (2b-RAD) approach was performed in 20 specimens from four communities from Manabí (central coast) and Loja (southern) provinces of Ecuador, and the effectiveness of three type IIB restriction enzymes was assessed. The findings of this study show that this genotyping methodology is cost effective in R. ecuadoriensis and likely in other triatomine species. In addition, preliminary population genomic analysis results detected a signal of population structure among geographically distinct communities and genetic variability within communities. As such, 2b-RAD shows significant promise as a relatively low-tech solution for determination of vector population genomics, dynamics, and spread

Effects of exercise training on airway responsiveness and airway cells in healthy subjects

J Appl Physiol. 2010 Aug;109(2):288-94. Epub 2010 Jun 10. Effects of exercise training on airway responsiveness and airway cells in healthy subjects. Scichilone N, Morici G, Zangla D, Chimenti L, Dav\uec E, Reitano S, Patern\uf2 A, Santagata R, Togias A, Bellia V, Bonsignore MR. SourceDept. of Internal Medicine, Div. of Pulmonology (DIBIMIS Univ. of Palermo, "Villa Sofia-Cervello" Hospital, Via Trabucco 180, 90146 Palermo, Italy. [email protected] Abstract Airway responsiveness to methacholine (Mch) in the absence of deep inspirations (DIs) is lower in athletes compared with sedentary individuals. In this prospective study, we tested the hypothesis that a training exercise program reduces the bronchoconstrictive effect of Mch. Ten healthy sedentary subjects (M/F: 3/7; mean + or - SD age: 22 + or - 3 yr) entered a 10-wk indoor rowing exercise program on rowing ergometer and underwent Mch bronchoprovocation in the absence of DIs at baseline, at weeks 5 and 10, as well as 4-6 wk after the training program was completed. Exercise-induced changes on airway cells and markers of airway inflammation were also assessed by sputum induction and venous blood samples. Mean power output during the 1,000 m test was 169 + or - 49 W/stroke at baseline, 174 + or - 49 W/stroke at 5 wk, and 200 + or - 60 W/stroke at 10 wk of training (P < 0.05). The median Mch dose used at baseline was 50 mg/ml (range 25-75 mg/ml) and remained constant per study design. At the pretraining evaluation, the percent reduction in the primary outcome, the inspiratory vital capacity (IVC) after inhalation of Mch in the absence of DIs was 31 +/- 13%; at week 5, the Mch-induced reduction in IVC was 22 + or - 19%, P = 0.01, and it further decreased to 15 + or - 11% at week 10 (P = 0.0008). The percent fall in IVC 4-6 wk after the end of training was 15 + or - 11% (P = 0.87 vs. end of training). Changes in airway cells were not associated with changes in airway responsiveness. Our data show that a course of exercise training can attenuate airway responsiveness against Mch inhaled in the absence of DIs in healthy subjects and suggest that a sedentary lifestyle may favor development of airways hyperresponsiveness. Comment in J Appl Physiol. 2010 Aug;109(2):267-8. PMID:20538849[PubMed - in process

Maximal Acceleration Is Nonrotating

In a stationary axisymmetric spacetime, the angular velocity of a stationary observer that Fermi-Walker transports its acceleration vector is also the angular velocity that locally extremizes the magnitude of the acceleration of such an observer, and conversely if the spacetime is also symmetric under reversing both t and phi together. Thus a congruence of Nonrotating Acceleration Worldlines (NAW) is equivalent to a Stationary Congruence Accelerating Locally Extremely (SCALE). These congruences are defined completely locally, unlike the case of Zero Angular Momentum Observers (ZAMOs), which requires knowledge around a symmetry axis. The SCALE subcase of a Stationary Congruence Accelerating Maximally (SCAM) is made up of stationary worldlines that may be considered to be locally most nearly at rest in a stationary axisymmetric gravitational field. Formulas for the angular velocity and other properties of the SCALEs are given explicitly on a generalization of an equatorial plane, infinitesimally near a symmetry axis, and in a slowly rotating gravitational field, including the weak-field limit, where the SCAM is shown to be counter-rotating relative to infinity. These formulas are evaluated in particular detail for the Kerr-Newman metric. Various other congruences are also defined, such as a Stationary Congruence Rotating at Minimum (SCRAM), and Stationary Worldlines Accelerating Radially Maximally (SWARM), both of which coincide with a SCAM on an equatorial plane of reflection symmetry. Applications are also made to the gravitational fields of maximally rotating stars, the Sun, and the Solar System.Comment: 64 pages, no figures, LaTeX, Sections 10 and 11 added with applications to maximally rotating stellar models of Cook, Shapiro, and Teukolsky and to the Sun and Solar System with recent data from Pijpers that the Sun has angular momentum 1.80 x 10^{75} = 0.216 M^2 = 47 hectares = 116 acres (with 0.8% uncertainty) and quadrupole moment (2.18 x 10^{-7})MR^2 = 1.60 x 10^{14} m^3 = 3.7 x 10^{117} (with 3% uncertaity), accepted Feb. 27 for Classical and Quantum Gravit

Optical pulse labeling studies reveal exogenous seeding slows α-synuclein clearance

The accumulation of α-synuclein (α-syn) in intracellular formations known as Lewy bodies (LBs) is associated with several neurodegenerative diseases including Parkinson's disease and Lewy Body Dementia. There is still limited understanding of how α-syn and LB formation is associated with cellular dysfunction and degeneration in these diseases. To examine the clearance and production dynamics of α-syn we transduced organotypic murine brain slice cultures (BSCs) with recombinant adeno-associated viruses (rAAVs) to express Dendra2-tagged human wild-type (WT) and mutant A53T α-syn, with and without the addition of exogenous α-syn fibrillar seeds and tracked them over several weeks in culture using optical pulse labeling. We found that neurons expressing WT or mutant A53T human α-syn show similar rates of α-syn turnover even when insoluble, phosphorylated Ser129 α-syn has accumulated. Taken together, this data reveals α-syn aggregation and overexpression, pSer129 α-syn, nor the A53T mutation affect α-syn dynamics in this system. Prion-type seeding with exogenous α-syn fibrils significantly slows α-syn turnover, in the absence of toxicity but is associated with the accumulation of anti-p62 immunoreactivity and Thiazin Red positivity. Prion-type induction of α-syn aggregation points towards a potential protein clearance deficit in the presence of fibrillar seeds and the ease of this system to explore precise mechanisms underlying these processes. This system facilitates the exploration of α-syn protein dynamics over long-term culture periods. This platform can further be exploited to provide mechanistic insight on what drives this slowing of α-syn turnover and how therapeutics, other genes or different α-syn mutations may affect α-syn protein dynamics

RELICS: The Reionization Lensing Cluster Survey and the Brightest High-z Galaxies

Massive foreground galaxy clusters magnify and distort the light of objects behind them, permitting a view into both the extremely distant and intrinsically faint galaxy populations. We present here the z ~ 6-8 candidate high-redshift galaxies from the Reionization Lensing Cluster Survey (RELICS), a Hubble and Spitzer Space Telescope survey of 41 massive galaxy clusters spanning an area of ≈200 arcmin². These clusters were selected to be excellent lenses, and we find similar high-redshift sample sizes and magnitude distributions as the Cluster Lensing And Supernova survey with Hubble (CLASH). We discover 257, 57, and eight candidate galaxies at z ~ 6, 7, and 8 respectively, (322 in total). The observed (lensed) magnitudes of the z ~ 6 candidates are as bright as AB mag ~23, making them among the brightest known at these redshifts, comparable with discoveries from much wider, blank-field surveys. RELICS demonstrates the efficiency of using strong gravitational lenses to produce high-redshift samples in the epoch of reionization. These brightly observed galaxies are excellent targets for follow-up study with current and future observatories, including the James Webb Space Telescope

A Quasi-Model-Independent Search for New Physics at Large Transverse Momentum

We apply a quasi-model-independent strategy ("Sleuth") to search for new high p_T physics in approximately 100 pb^-1 of ppbar collisions at sqrt(s) = 1.8 TeV collected by the DZero experiment during 1992-1996 at the Fermilab Tevatron. Over thirty-two e mu X, W+jets-like, Z+jets-like, and 3(lepton/photon)X exclusive final states are systematically analyzed for hints of physics beyond the standard model. Simultaneous sensitivity to a variety of models predicting new phenomena at the electroweak scale is demonstrated by testing the method on a particular signature in each set of final states. No evidence of new high p_T physics is observed in the course of this search, and we find that 89% of an ensemble of hypothetical similar experimental runs would have produced a final state with a candidate signal more interesting than the most interesting observed in these data.Comment: 28 pages, 17 figures. Submitted to Physical Review