97 research outputs found
Analysis of influenza a virus polypeptides by limited proteolysis in SDS-polyacrylamide gels.
Dept. of Biological Sciences. Paper copy at Leddy Library: Theses & Major Papers - Basement, West Bldg. / Call Number: Thesis1985 .P379. Source: Masters Abstracts International, Volume: 40-07, page: . Thesis (M.Sc.)--University of Windsor (Canada), 1985
Correction to “Analysis of the summertime buildup of tropospheric ozone abundances over the Middle East and North Africa as observed by the Tropospheric Emission Spectrometer Instrument”
VALIDATION OF INERTIAL MEASUREMENT UNITS FOR TRACKING 100M SPRINT DATA
Wearable micro sensor measurement devices are a promising development in sports technology. This paper presents preliminary data evaluating the accuracy of an inertial measurement unit during 100m sprints against a criterion measure from a tripod-mounted Laveg laser. The inertial measurement units were found to be a valid tool for the analysis of peak velocity (r = 0.92) and average split velocities for splits after the first 10m (r = 0.85 - 0.95). Validation data suggests some caution should be taken in interpretation of the first lorn split (r = 0.32). Whilst data from the two devices for this split were correlated, the inertial measurement unit showed an overestimation for this parameter in comparison to the athlete velocity as measured by the laser. Further in-depth analysis should investigate this period
User requirements for monitoring the evolution of stratospheric ozone at high vertical resolution (‘Operoz’: Operational ozone observations using limb geometry)
The purpose of the Operoz study has been threefold:
(i) To establish the user requirements for an operational mission targeting ozone profiles at high vertical resolution,
(ii) To identify the observational gaps with respect to these user requirements taking into account planned operational missions and observational ground networks, and
(iii) To perform a reality check on the observational requirements based on proven concepts and present-day knowledge of potentially available measurement techniques and to identify options for a small to medium size satellite mission
An emerging role for NAADP-mediated Ca2+ signaling in the pancreatic beta-cell
Several recent reports, including one in this journal, have reignited the debate about whether the calcium-mobilizing messenger, nicotinic adenine nucleotide diphosphate (NAADP) plays a central role in the regulation of calcium signalling in pancreatic β-cell. These studies have highlighted a role for NAADP-induced Ca(2+) mobilization not only in mediating the effects of the incretin, GLP-1 and the autocrine proliferative effects of insulin, but also possibly a fundamental role in glucose-mediated insulin secretion in the pancreatic β-cell
Estimating Wildfire-Generated Ozone over North America Using Ozonesonde Profiles and a Differential Back Trajectory Technique
An objective method, employing HYSPLIT back-trajectories and Moderate Resolution Imaging Spectroradiometer (MODIS) fire observations, is developed to estimate ozone enhancement in air transported from regions of active forest fires at 18 ozone sounding sites located across North America. The Differential Back Trajectory (DBT) method compares mean differences between ozone concentrations associated with fire-affected and fire-unaffected parcels. It is applied to more than 1100 ozonesonde profiles collected from these sites during the summer months June to August 2006, 2008, 2010 and 2011. Layers of high ozone associated with low humidity were first removed from the ozonesonde profiles to minimize the potential effects of stratospheric intrusions on the calculations. No significant influence on average ozone levels by North American fires was found for stations located at Arctic latitudes. The ozone enhancement for stations nearer large fires, such as Trinidad Head and Bratt\u27s Lake, was up to 4.8% of the TTOC (Total Tropospheric Ozone Column). Fire ozone accounted for up to 8.3% of TTOC at downwind sites such as Yarmouth, Sable Island, Narragansett, and Walsingham. The results are consistent with other studies that have reported an increase in ozone production with the age of the smoke plume
Unprecedented atmospheric ammonia concentrations detected in the high Arctic from the 2017 Canadian wildfires
Abstract From 17-22 August 2017 simultaneous enhancements of ammonia (NH3), carbon monoxide (CO), hydrogen cyanide (HCN), and ethane (C2H6) were detected from ground-based solar absorption Fourier transform infrared (FTIR) spectroscopic measurements at two high-Arctic sites: Eureka (80.05°N, 86.42°W) Nunavut, Canada and Thule (76.53°N, 68.74°W), Greenland. These enhancements were attributed to wildfires in British Columbia and the Northwest Territories of Canada using FLEXPART back-trajectories and fire locations from Moderate Resolution Imaging Spectroradiometer (MODIS) and found to be the greatest observed enhancements in more than a decade of measurements at Eureka (2006-2017) and Thule (1999-2017). Observations of gas-phase NH3 from these wildfires illustrates that boreal wildfires may be a considerable episodic source of NH3 in the summertime high Arctic. Comparisons of GEOS-Chem model simulations using the Global Fire Assimilation System (GFASv1.2) biomass burning emissions to FTIR measurements and Infrared Atmospheric Sounding Interferometer (IASI) measurements showed that the transport of wildfire emissions to the Arctic was underestimated in GEOS-Chem. However, GEOS-Chem simulations showed that these wildfires contributed to surface-layer NH3 and enhancements of 0.01-0.11 ppbv and 0.05-1.07 ppbv, respectively, over the Canadian Archipelago from 15-23 August 2017
Both RyRs and TPCs are required for NAADP-induced intracellular Ca2+ release
Intracellular Ca2+ release is mostly mediated by inositol trisphosphate, but intracellular cyclic-ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP) are important messengers in many systems. Whereas cADPR generally activates type 2 ryanodine receptors (RyR2s), the NAADP-activated Ca2+ release mechanism is less clear. Using knockouts and antibodies against RyRs and Two-Pore Channels (TPCs), we have compared their relative importance for NAADP-induced Ca2+ release from two-photon permeabilized pancreatic acinar cells. In these cells, cholecystokinin-elicited Ca2+ release is mediated by NAADP. TPC2-KO reduced NAADP-induced Ca2+ release by 64%, but the combination of TPC2-KO and an antibody against TPC1, significantly reduced Ca2+ release by 86% (64% vs. 86%, p TPC2 > RyR3 > TPC1 >> RyR2. However, when assessing NAADP-induced Ca2+ release solely from the acidic stores (granules/endosomes/lysosomes), antibodies against TPC2 and TPC1 virtually abolished the Ca2+ liberation as did antibodies against RyR1 and RyR3. Our results indicate that the primary, but very small, NAADP-elicited Ca2+ release via TPCs from endosomes/lysosomes triggers the detectable Ca2+-induced Ca2+ release via RyR1 and RyR3 occurring from the granules and the ER
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