897 research outputs found

    Estimates of combining ability and heterosis for growth traits in a full diallel cross of three strains of common carp, Cyprinus carpio L.

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    We attempted to determine the combining ability and heterosis of body weight by performing 3 × 3 complete diallel crosses derived by crossing three common carps (Cyprinus carpio var. jian, Cyprinus carpio haematopterus Temminck et Schlegel and Cyprinus carpio haematopterus). In total, 1650 fish were tagged when they were 1.5 months of age. After five months, Jian carp (♂) × Huanghe carp (♀) attained greater weight than pure and other hybrids. By crossing with Jian, Huanghe and Heilongjiang carps have improved body weight compared to their purebred. Significant principal components (PCs) were generated from growth performance data and their statistical loadings. The first principal component had the highest Eigen value of 2.81 and accounted for 70.31% of the variability in the data set. Two crosses, Jian carp (♂) × Huanghe carp (♀) and Huanghe carp (♂) × Jian carp (♀) showed high SCA with regard to body weight. Both Heilongjiang carp (♂) × Jian carp (♀) and Heilongjiang carp (♂) × Huanghe carp (♀) had higher heterosis compared to their purebreds. Significant positive phenotypic correlations were also observed between body weight and body length, thickness and height. The aforementioned results listed were identified as the foundation for common carp selective breeding programs.Keywords: Common carp, cross-breeding, heterosisAfrican Journal of Biotechnology Vol. 12(22), pp. 3514-352

    Mechanism of glycan receptor recognition and specificity switch for avian, swine, and human adapted influenza virus hemagglutinins: a molecular dynamics perspective.

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    Hemagglutinins (HA's) from duck, swine, and human influenza viruses have previously been shown to prefer avian and human glycan receptor analogues with distinct topological profiles, pentasaccharides LSTa (alpha-2,3 linkage) and LSTc (alpha-2,6 linkage), in comparative molecular dynamics studies. On the basis of detailed analyses of the dynamic motions of the receptor binding domains (RBDs) and interaction energy profiles with individual glycan residues, we have identified approximately 30 residue positions in the RBD that present distinct profiles with the receptor analogues. Glycan binding constrained the conformational space sampling by the HA. Electrostatic steering appeared to play a key role in glycan binding specificity. The complex dynamic behaviors of the major SSE and trimeric interfaces with or without bound glycans suggested that networks of interactions might account for species specificity in these low affinity and high avidity (multivalent) interactions between different HA and glycans. Contact frequency, energetic decomposition, and H-bond analyses revealed species-specific differences in HA-glycan interaction profiles, not readily discernible from crystal structures alone. Interaction energy profiles indicated that mutation events at the set of residues such as 145, 156, 158, and 222 would favor human or avian receptor analogues, often through interactions with distal asialo-residues. These results correlate well with existing experimental evidence, and suggest new opportunities for simulation-based vaccine and drug development

    Effects of Antibacterial Peptide Extracted from Bacillus subtilis fmbJ on the Growth, Physiological Response and Disease Resistance of Megalobrama amblycephala

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    The effects of an antibacterial peptide obtained from Bacillus subtilis fmbJ on growth, serum lysozyme complements 3 and 4, total protein content, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total antioxidative capacity, superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and disease resistance of Wuchang bream (Megalobrama amblycephala) were examined. Fish were randomly divided into five groups: a control group which was fed a basic diet, and four groups fed the basic diet supplemented with 0.1%, 0.2%, 0.4%, or 0.8% antibacterial peptide. At eight weeks, M. amblycephala fed the diet containing 0.2% antibacterial peptide had higher serum lysozyme activity, complement 3 and 4 contents, and SOD activity than the control fish, but lower serum MDA content and AST activity. Fish fed the 0.4% diet had higher weight gain rate, serum lysozyme activity, complement 4 content, total antioxidative capacity, and total protein than the control, and lower serum ALT activity. Feed conversion ratios of fish fed the 0.2% or 0.4% diets were lower than those of control fish. Artificial infection with Aeromonas hydrophila resulted in 93% cumulative mortality in the control group, and 61-84% in the groups fed the 0.2% or 0.4% diets. The present study suggests that feed supplementation with 0.2-0.4% antibacterial peptides can stimulate immunity, increase resistance to pathogenic infection, and promote growth in M. amblycephala

    Effects of Dietary Protein Level on Growth Performance, Muscle Composition, Blood Composition, and Digestive Enzyme Activity of Wuchang Bream (Megalobrama amblycephala) Fry

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    The purpose of this study was to determine the dietary protein requirement and effects of dietary protein level on growth performance, muscle composition, blood composition, and digestive enzyme activity in Wuchang bream fry. Five isoenergetic and isolipidic semi-purified diets were formulated to contain 28%, 30%, 32%, 34%, or 36% (dry matter) dietary protein. Diets were fed to triplicate groups of 25 fishes (16.08±0.03 g) to near satiation three times a day in a closed recirculation system for 10 weeks. Weight gain, specific growth rate, and feed conversion ratio significantly improved as the dietary protein content increased up to 34%. The protein efficiency ratio, hepatosomatic index, and viscerosomatic index significantly dropped as the dietary protein rose while the Fulton condition factor was positively correlated to the dietary protein level. Increased dietary protein resulted in increased muscle protein content and decreased lipid content. Red blood cell, hemoglobin, and hematocrit counts increased significantly with the increase in dietary protein. Serum triiodothyronine and thyroxine significantly rose as the dietary protein rose but serum aspartate aminotransferase significantly dropped. Intestinal protease and amylase activity rose significantly with the increase in dietary protein while lipase tended to drop. On the basis of broken-line regression analysis of weight gain and FCR, the dietary protein requirement of Wuchang bream fry is 32-33%

    Theory of Nonlinear Meissner Effect in High-Tc Superconductors

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    We investigate the nonlinear Meissner effect microscopically. Previous studies did not consider a certain type of interaction effect on the nonlinear phenomena. The scattering amplitude barely appears without being renormalized into the Fermi-liquid parameter. With this effect we can solve the outstanding issues (the quantitative problem, the temperature and angle dependences). The quantitative calculation is performed with use of the fluctuation-exchange approximation on the Hubbard model. It is also shown that the perturbation expansion on the supercurrent by the vector potential converges owing to the nonlocal effect

    Gut Microbes Reveal Pseudomonas Medicates Ingestion Preference via Protein Utilization and Cellular Homeostasis Under Feed Domestication in Freshwater Drum, Aplodinotus grunniens

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    With strong demand for aquatic products, as well as a rapid decrease in global fishery resources and capture fisheries, domesticating animals to provide more high-quality proteins is meaningful for humans. Freshwater drum (Aplodinotus grunniens) is widely distributed in the wild habitats of North America. However, the research on A. grunniens and the feed domestication with diets composed of artificial compounds remains unclear. In this study, a 4-month feeding domestication experiment was conducted with A. grunniens larvae to evaluate the underlying mechanism and molecular targets responsible for alternations in the ingestion performance. The results indicated that a significant increase in the final body weight was exhibited by the feed domesticated group (DOM, 114.8 g) when compared to the group that did not ingest the feed (WT, 5.3 g) as the latest version we raised From the result, the final body weight exhibited significant increase between unfavorable with the feed (WT, 5.3 g) and feed domesticated group (DOM, 114.8 g). In addition, the enzyme activity of digestive enzymes like amylase, lipase, and trypsin was increased in DOM. Genes related to appetite and perception, such as NPY4R, PYY, and LEPR, were activated in DOM. 16s rRNA gene sequencing analysis revealed that Pseudomonas sp. increased from 58.74% to 89.77% in DOM, which accounts for the dominant upregulated microbial community at the genus level, followed by Plesiomonas. Analogously, Mycobacterium, Methylocystis, and Romboutsia also accounted for the down-regulated microbes in the diversity. Transcriptome and RT-PCR analysis revealed that feed domestication significantly improved protein digestion and absorption, inhibited apoptosis by AGE-RAGE signaling, and activated extracellular matrix remodeling by relaxin signaling. Integrated analysis of the microbiome and host transcriptome revealed that Pseudomonas-mediated ingestion capacity, protein utilization, and cellular homeostasis might be the underlying mechanism under feed domestication. These results indicate Pseudomonas and its key genes relating to food ingestion and digestion could serve as the molecular targets for feed domestication and sustainable development in A. grunniens

    Coexistence and optimal control problems for a degenerate predator-prey model

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    In this paper we present a predator-prey mathematical model for two biological populations which dislike crowding. The model consists of a system of two degenerate parabolic equations with nonlocal terms and drifts. We provide conditions on the system ensuring the periodic coexistence, namely the existence of two non-trivial non-negative periodic solutions representing the densities of the two populations. We assume that the predator species is harvested if its density exceeds a given threshold. A minimization problem for a cost functional associated with this process and with some other significant parameters of the model is also considered. \ua9 2010 Elsevier Inc

    A chromosomelevel genome assembly of the Asian arowana, Scleropages formosus

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    Asian arowana (Scleropages formosus), an ancient teleost belonging to the Order Osteoglossomorpha, has been a valuable ornamental fish with some varieties. However, its biological studies and breeding germplasm have been remarkably limited by the lack of a reference genome. To solve these problems, here we report high-quality genome sequences of three common varieties of Asian arowana (the golden, red and green arowana). We firstly generated a chromosome-level genome assembly of the golden arowana, on basis of the genetic linkage map constructed with the restriction site-associated DNA sequencing (RAD-seq). In addition, we obtained draft genome assemblies of the red and green varieties. Finally, we annotated 22,016, 21,256 and 21,524 protein-coding genes in the genome assemblies of golden, red and green varieties respectively. Our data were deposited in publicly accessible repositories to promote biological research and molecular breeding of Asian arowana

    Changes in Physiological Parameters, Lipid Metabolism, and Expression of MicroRNAs in Genetically Improved Farmed Tilapia (Oreochromis niloticus) With Fatty Liver Induced by a High-Fat Diet

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    Tilapia is susceptible to hepatic steatosis when grown in intensive farming systems. The aim of this study was to explore the mechanism of fatty liver induced by a high-fat diet (HFD) in genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Juvenile GIFT were fed with HFD or a normal-fat diet (NFD) for 60 days. Substantial fat deposition in the liver of HFD-fed GIFT on days 20, 40, and 60 was observed using hematoxylin – eosin staining and oil red O staining. The increased fat deposition was consistent with increased triglyceride (TG) and total cholesterol (TC) levels in the liver of HFD-fed GIFT. There were significant differences (P < 0.05) in serum biochemical indexes (TG, TC, low density lipoprotein-cholesterol, and insulin contents, and alanine aminotransferase activity) between GIFT fed a HFD and GIFT fed a NFD on days 20, 40, and 60. Furthermore, 60 days of a HFD significantly changed (P < 0.05) the hepatic fatty acid composition, and led to increased polyunsaturated fatty acid levels and decreased saturated fatty acid and monounsaturated fatty acid levels. Hepatic antioxidant enzyme activities increased by day 20 and then declined, which led to an increase in malondialdehyde contents in the liver of HFD-fed GIFT. Molecular analyses revealed that the microRNAs miR-122, miR-29a, and miR-145-5p were upregulated, whereas miR-34a was downregulated in HFD-fed GIFT. SCD, ELOVL6, and SRD5A2 encode three important enzymes in lipid metabolism, and were identified as potential targets of miRNAs. The transcript levels of hepatic SCD and ELOVL6 were decreased and that of hepatic SRD5A2 was increased in GIFT fed a HFD. Overall, the results of this study revealed a potential link between miRNAs and fatty liver induced by HFD, and suggest that a HFD could lead to excess fat deposition in the GIFT liver, which may disrupt hepatic lipid metabolism and reduce the antioxidant defense capacity
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