A prospective survey on knowledge, attitude and current practices of pre-operative fasting amongst anaesthesiologists: A nationwide survey

Background and Aims: Pre-operative fasting is a routine practice to minimise the risk of pulmonary aspiration. The leading societies of anaesthesia have adopted more liberal fasting guidelines to avoid the adverse effect of prolonged fasting. This survey was conducted to assess the knowledge, attitude and current practice of fasting guidelines among Indian anaesthesiologists and to analyse the reasons for non-compliance of these guidelines. Methods: A questionnaire consisted of 11 questions was distributed via 'Survey Monkey' software to 621 anaesthesiologists who attended the annual conference of the Indian Society of Anaesthesiologists held in Ludhiana, in 2016. American Society of Anaesthesiologists (ASA) practice guidelines for fasting were the standard of assessment. Results: The response rate to the survey was 52%. Of the respondents, 69% described correctly the practice guidelines to pre-operative fasting. Only seven percent respondents were aware of the benefits of liberalised fasting. More than 2/3rd of the respondents advised fasting as per ASA guidelines during pre-anaesthetic check-up (PAC). However, only about 50% respondents confirmed that these guidelines are actually followed in their institution. Not having control on scheduling of cases in operation theatre and poor knowledge of ward nurses and surgeons were the common reasons for non-compliance of these guidelines. Twenty four percent respondents did not use routinely any drug for aspiration prophylaxis. Conclusion: Majority of the respondents were aware of the ASA fasting guidelines. However, the implementation of the guidelines and knowledge regarding benefits of liberalised fasting is poor among respondents

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Not AvailableThe biodiversity of wheat-associated bacteria from the northern hills zone of India was deciphered. A total of 247 bacteria was isolated from five different sites. Analysis of these bacteria by amplified ribosomal DNA restriction analysis (ARDRA) using three restriction enzymes, AluI, MspI and HaeIII, led to the grouping of these isolates into 19–33 clusters for the different sites at 75 % similarity index. 16S rRNA gene based phylogenetic analysis revealed that 65 %, 26 %, 8 % and 1 % bacteria belonged to four phyla, namely Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, respectively. Overall, 28 % of the total morphotypes belonged to Pseudomonas followed by Bacillus (20 %), Stenotrophomonas (9 %), Methylobacterium (8 %), Arthrobacter (7 %), Pantoea (4 %), Achromobacter, Acinetobacter, Exiguobacterium and Staphylococcus (3 %), Enterobacter, Providencia, Klebsiella and Leclercia (2 %), Brevundimonas, Flavobacterium, Kocuria, Kluyvera and Planococcus (1 %). Representative strains from each cluster were screened in vitro for plant growth promoting traits, which included solubilisation of phosphorus, potassium and zinc; production of ammonia, hydrogen cyanide, indole-3-acetic acid and siderophore; nitrogen fixation, 1-aminocyclopropane-1-carboxylate deaminase activity and biocontrol against Fusarium graminearum, Rhizoctonia solani and Macrophomina phaseolina. Cold-adapted isolates may have application as inoculants for plant growth promotion and biocontrol agents for crops growing under cold climatic conditions.Not Availabl

Intestinal bacterial flora of Mediterranean gilthead sea bream ( Sparus aurata

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