611 research outputs found
Transmission measurement at 10.6 microns of Te2As3Se5 rib-waveguides on As2S3 substrate
The feasibility of chalcogenide rib waveguides working at lambda = 10.6
microns has been demonstrated. The waveguides comprised a several microns thick
Te2As3Se5 film deposited by thermal evaporation on a polished As2S3 glass
substrate and further etched by physical etching in Ar or CF4/O2 atmosphere.
Output images at 10.6 microns and some propagation losses roughly estimated at
10dB/cm proved that the obtained structures behaved as channel waveguides with
a good lateral confinement of the light. The work opens the doors to the
realisation of components able to work in the mid and thermal infrared up to 20
microns and even more.Comment: The following article appeared in Vigreux-Bercovici et al., Appl.
Phys. Lett. 90, 011110 (2007) and may be found at
http://link.aip.org/link/?apl/90/01111
Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections
Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the development of the infection, but the mechanisms involved remain unclear. Many bacterial pathogens target the follicle-associated epithelium, which overlies Peyer's patches (PPs), cross the intestinal barrier through M cells and are captured by mucosal macrophages. Here, translocation across M cells, as well as survival and proliferation of EHEC strains within THP-1 macrophages were investigated using EHEC O157:H7 reference strains, isogenic mutants, and 15 EHEC strains isolated from HC/HUS patients. We showed for the first time that E. coli O157:H7 strains are able to interact in vivo with murine PPs, to translocate ex vivo through murine ileal mucosa with PPs and across an in vitro human M cell model. EHEC strains are also able to survive and to produce Stx in macrophages, which induce cell apoptosis and Stx release. In conclusion, our results suggest that the uptake of EHEC by M cells and underlying macrophages in the PP may be a critical step in Stx translocation and release in vivo. A new model for EHEC infection in humans is proposed that could help in a fuller understanding of EHEC-associated diseases
A Multidomain Adhesion Protein Family Expressed in Plasmodium falciparum Is Essential for Transmission to the Mosquito
The recent sequencing of several apicomplexan genomes has provided the opportunity to characterize novel antigens essential for the parasite life cycle that might lead to the development of new diagnostic and therapeutic markers. Here we have screened the Plasmodium falciparum genome sequence for genes encoding extracellular multidomain putative adhesive proteins. Three of these identified genes, named PfCCp1, PfCCp2, and PfCCp3, have multiple adhesive modules including a common Limulus coagulation factor C domain also found in two additional Plasmodium genes. Orthologues were identified in the Cryptosporidium parvum genome sequence, indicating an evolutionary conserved function. Transcript and protein expression analysis shows sexual stageâspecific expression of PfCCp1, PfCCp2, and PfCCp3, and cellular localization studies revealed plasma membraneâassociated expression in mature gametocytes. During gametogenesis, PfCCps are released and localize surrounding complexes of newly emerged microgametes and macrogametes. PfCCp expression markedly decreased after formation of zygotes. To begin to address PfCCp function, the PfCCp2 and PfCCp3 gene loci were disrupted by homologous recombination, resulting in parasites capable of forming oocyst sporozoites but blocked in the salivary gland transition. Our results describe members of a conserved apicomplexan protein family expressed in sexual stage Plasmodium parasites that may represent candidates for subunits of a transmission-blocking vaccine
Technology challenges for space interferometry: the option of mid-infrared integrated optics
Nulling interferometry is a technique providing high angular resolution which
is the core of the space missions Darwin and the Terrestrail Planet Finder. The
first objective is to reach a deep degree of starlight cancelation in the range
6 -- 20 microns, in order to observe and to characterize the signal from an
Earth-like planet. Among the numerous technological challenges involved in
these missions, the question of the beam combination and wavefront filtering
has an important place. A single-mode integrated optics (IO) beam combiner
could support both the functions of filtering and the interferometric
combination, simplifying the instrumental design. Such a perspective has been
explored in this work within the project Integrated Optics for Darwin (IODA),
which aims at developing a first IO combiner in the mid-infrared. The solutions
reviewed here to manufacture the combiner are based on infrared dielectric
materials on one side, and on metallic conductive waveguides on the other side.
With this work, additional inputs are offered to pursue the investigation on
mid-infrared photonics devices.Comment: Accepted in Adv. in Space Researc
A symmoriiform chondrichthyan braincase and the origin of chimaeroid fishes
Chimaeroid fishes (Holocephali) are one of the four principal divisions of modern gnathostomes (jawed vertebrates). Despite only 47 described living species1, chimaeroids are the focus of resurgent interest as potential archives of genomic data2 and for the unique perspective they provide on chondrichthyan and gnathostome ancestral conditions. Chimaeroids are also noteworthy for their highly derived body plan1,3,4. However, like other living groups with distinctive anatomies5, fossils have been of limited use in unravelling their evolutionary origin, as the earliest recognized examples already exhibit many of the specializations present in modern forms6,7. Here we report the results of a computed tomography analysis of Dwykaselachus, an enigmatic chondrichthyan braincase from the ~280 million year old Karoo sediments of South Africa8. Externally, the braincase is that of a symmoriid shark9,10,11,12,13and is by far the most complete uncrushed example yet discovered. Internally, the morphology exhibits otherwise characteristically chimaeroid specializations, including the otic labyrinth arrangement and the brain space configuration relative to exceptionally large orbits. These results have important implications for our view of modern chondrichthyan origins, add robust structure to the phylogeny of early crown group gnathostomes, reveal preconditions that suggest an initial morpho-functional basis for the derived chimaeroid cranium, and shed new light on the chondrichthyan response to the extinction at the end of the Devonian period
From local monitoring to a broadâscale viability assessment: a case study for the Bonelli's Eagle in western Europe
Population viability analysis (PVA) has become a basic tool of current conservation practice. However, if not accounted for properly, the uncertainties inherent to PVA predictions can decrease the reliability of this type of analysis. In the present study, we performed a PVA of the whole western European population (France, Portugal, and Spain) of the endangered Bonelli's Eagle (Aquila fasciata), in which we thoroughly explored the consequences of uncertainty in population processes and parameters on PVA predictions. First, we estimated key vital rates (survival, fertility, recruitment, and dispersal rates) using monitoring, ringing, and bibliographic data from the period 1990-2009 from 12 populations found throughout the studied geographic range. Second, we evaluated the uncertainty about model structure (i.e., the assumed processes that govern individual fates and population dynamics) by comparing the observed growth rates of the studied populations with model predictions for the same period. Third, using the model structures suggested in the previous step, we assessed the viability of both the local populations and the overall population. Finally, we analyzed the effects of model and parameter uncertainty on PVA predictions. Our results strongly support the idea that all local populations in western Europe belong to a single, spatially structured population operating as a source-sink system, whereby the populations in the south of the Iberian Peninsula act as sources and, thanks to dispersal, sustain all other local populations, which would otherwise decline. Predictions regarding population dynamics varied considerably, and models assuming more constrained dispersal predicted more pessimistic population trends than models assuming greater dispersal. Model predictions accounting for parameter uncertainty revealed a marked increase in the risk of population declines over the next 50 years. Sensitivity analyses indicated that adult and preâadult survival are the chief vital rates regulating these populations, and thus, the conservation efforts aimed at improving these survival rates should be strengthened in order to guarantee the longâterm viability of the European populations of this endangered species. Overall, the study provides a framework for the implementation of multiâsite PVAs and highlights the importance of dispersal processes in shaping the population dynamics of longâlived birds distributed across heterogeneous landscapes
Incorporating ârecruitmentâ in matrix projection models : estimation, parameters, and the influence of model structure
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Journal of Ornithology 152, Suppl.2 (2012):585-595, doi:10.1007/s10336-010-0573-1.Advances in the estimation of population parameters
using encounter data from marked individuals have
made it possible to include estimates of the probability of
recruitment in population projection models. However, the
projected growth rate of the population, and the sensitivity
of projected growth to changes in recruitment, can vary
significantly depending upon both the structural form of the
model and how recruitment is parameterized. We show that
the common practices of (1) collapsing some age classes
into a single, terminal âaggregatedâ age-class, and (2) parameterizing
recruitment using the proportion of recruited
individuals (breeders) in a given age-class may confound
analysis of age-based (Leslie) matrix projection models in
some instances, relative to state-based projection models
where recruited and pre-recruited individuals are treated as
separate states. Failing to account for these differences can
lead to misinterpretation of the relative role of recruitment in
the dynamics of an age-structured population.We show that
such problems can be avoided, either by structural changes
to the terminal aggregated age-class in age-based models,
or by using using a state-based model instead. Since all
the metrics of general interest from a classical age-based
matrix models are readily derived from a state-based model
equivalent, this suggests there may be little reason to use the
classical age-based approach in situations where recruitment
is a parameter of interest
West Nile Virus Surveillance, Guadeloupe, 2003â2004
We conducted extensive surveillance for West Nile virus infection in equines and chickens in Guadeloupe in 2003â2004. We showed a high seroprevalence in equines in 2003 related to biome, followed by a major decrease in virus circulation in 2004. No human or equine cases were reported during the study
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