Parameters of oxidative stress in saliva from patients with aggressive and chronic periodontitis

Objectives: Free radicals play an important role in the onset and progression of many diseases. The aim ofthis study was to investigate the contribution of oxidative stress in the pathology of aggressive (AgP) andchronic (CP) periodontitis and its relation with the clinical periodontal status.Methods: Eighty subjects were divided into two groups: 20 patients with AgP and 20 patients with CP withtheir 20 corresponding matched controls, based on clinical attachment loss (CAL), probing pocket depth(PPD), and bleeding on probing (BOP). Saliva reactive oxygen species (ROS), lipid peroxidation, and nonenzymaticantioxidant defences were measured by luminol-dependent chemiluminescence assay, asthiobarbituric acid-reactive substances (TBARs) and total radical-trapping antioxidant potential (TRAP),respectively. Pearson?s correlation and multivariate analysis were used to determine the relationshipbetween ROS and TBARs and the clinical parameters.Results: ROS and TBARs were increased in AgP while TRAP was decreased, comparing with CP. In AgP, astrong and positive correlation was observed between ROS and TBARs and they were closely associatedwith CAL and PPD.Discussion: In AgP, but not in CP, oxidative stress is a high contributor to periodontal pathology and it isclosely associated with the clinical periodontal status.Fil: Acquier, Andrea Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: de Couto Pita, Alejandra Karina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Busch, Lucila. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Sanchez, Gabriel A.. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Biofísica; Argentin

The territorial dimension of electrical power consumption in Andalusia at detailed scales

El trabajo analiza las pautas espaciales seguidas por el consumo de energía eléctrica en los municipios andaluces. Sobre los 661 municipios que reciben suministro eléctrico de Sevillana-ENDESA, se toman en consideración 11 variables alusivas al consumo eléctrico total y sectorial y a factores económicos y demográfi cos determinantes de aquéllos. Su análisis permite observar la plasmación espacial de los consumos y realizar una tipología de los municipios con arreglo a su comportamiento consumidor. Como resultado de la tipología se establecen cinco tipos diferentes de municipios en la comunidad andaluza.This paper analyzes the spatial patterns followed by the consumption of electricity in the Andalusian municipalities. 11 variables related to the total and sectorial power consumption as well as economic and demographic factors of them have been taken into account in the 661 municipalities that receive electricity from Sevillana-ENDESA. Their analysis allows observing the spatial pattern of consumption and making a typology of municipalities according to their consumption behavior. As a result a typology with fi ve different types of municipalities is set out

Effect of blood flow restriction training on exercisee-induced muscle damage and sport performance

Traballo fin de grao (UDC.FCDEF). Ciencias da actividade física e do deporte. Curso 2018/201

Carbon and water vapor fluxes over four forests in two contrasting climatic zones

AbstractThe inter- and seasonal patterns of water vapor and canopy carbon fluxes were compared for four forest ecosystems in two contrasting climatic zones in Europe. The eddy covariance and ancillary data were taken from the Carboeurope and FLUXNET databases and a linear modeling statistical analysis was made. The four sites were a high-density poplar (Populus spp.) short rotation coppice plantation (in Lochristi, Belgium) and a mature Scots pine (Pinus sylvestris) forest (in Brasschaat, Belgium) in the Temperate climate versus a fast-growing Eucalypt (Eucalyptus) plantation (in Espirra, Portugal) and a Holm oak (Quercus ilex) forest (in Puechabon, France) in the Mediterranean climate.•The Eucalypt stand showed an efficient stomatal control in response to changes in vapor pressure deficit (VPD), suggesting an ideal adaptation of this species to the severe Mediterranean climate.•The fast-growing poplar stand did not show a similar stomatal control under conditions of moderate water stress. But during an intensive dry period a decrease in the development of the leaf area index (LAI) was observed.•The Holm oak stand showed a low GPP, which is typical for a low productive species with a long rotation cycle. The GPP showed low diurnal variability, even under high solar radiation. This behavior suggested a strong stomatal control caused by the severe water stress, a mechanism that allowed this stand to cope with diurnal and seasonal water deficits.•The mature Scots pine forest in the Temperate climate showed no variation in the GPP – radiation relationship. In this forest no water stress was observed, probably because the trees always had access to the water table. Irrespective of the climate the evapotranspiration of the Scots pine forest presented a tight coupling with the atmosphere, i.e. a low decoupling factor, Ω, comparable with the Holm oak and the Eucalypt forests.The high Ω values of the young poplar plantation were not typical for forest canopies. These values confirmed the strong influence of solar radiation and available energy on evapotranspiration and on the dynamics of this fast-developing canopy. At all four sites the forests showed their capacity to react to the environmental drivers, characteristic from their respective climatic types. However, drastic climatic changes – such as heat waves or long drought spells – may compromise the productivity of fast-growing plantations such as the Eucalypt and poplar stands. The response of the poplars to these events is mainly achieved through LAI control in contrast to the stomatal control in the Eucalypts

Comparação do perfil de tarefa e capitanía nos líderes de times de futebol e basquete

La presente investigación pretende explorar la relación entre características de la tarea y el liderazgo, así como conocer si el ejercicio del liderazgo informal está alineado con la figura formal del capitán. Acerca del estudio de estas variables se ha tratado de conocer cómo puede influir sobre ellas el tipo de cultura de liderazgo según el deporte. Se aplicó la EELD-24 (Torrado y Arce, 2015), junto a preguntas destinadas a evaluar el perfil de tarea, a una muestra de 219 deportistas adultos masculinos (132 de fútbol y 87 de baloncesto). Se ha observado que el tipo de deporte condiciona dicho perfil de tarea y el ejercicio de la capitanía por parte de los líderes informales. En fútbol, menos de la mitad de los líderes informales son capitanes (44.2%), destacando en ellos en mayor medida la posición en el campo que su nivel de juego. En baloncesto, una gran parte de los líderes sí son capitanes (77.1%) pero en ellos influye mucho más el nivel de juego que la ocupación de funciones muy influyentes en el juego colectivo. Los resultados relativizan las conclusiones aportadas por investigaciones previas sobre la influencia del perfil de tarea (Moran y Weiss, 2006)Present research try to know about task characteristics and leadership, and also explore if peer leadership is lined up to formal leader as the captain. About this variable it was tried to know how leadership culture in sport can affect. The EELD-24 (Torrado & Arce, 2015), and questions oriented to evaluate task profile, were applied to a 219 men athletes sample (132 from soccer and 87 from basketball). It was observed that type of sport determines task profile and captaincy development of peer leaders. In soccer, minus than a half of peer leaders are captains (44.2%), standing out for them more the position in the field than level of performance. In basketball, most of leaders are captains (77.1), but in them is more important level of performance than position in the team structure during the game. Results diminish the importance of previous research about influence of task profile (Moran y Weiss, 2006)A presente investigação pretende explorar a relação entre características da tarefa e a liderança, bem como conhecer se o exercício da liderança informal está alinhado com a figura formal do capitão. A respeito do estudo destas variáveis tratou-se de conhecer como pode influir sobre elas o tipo de cultura de liderança segundo o desporto. Aplicou-se a EELD-24 (Torrado e Arce, 2015), mais questoes referidas ao perfil de tarefa, a uma mostra de 219 desportistas adultos masculinos (132 de futebol e 87 de basquete). Observou-se que o tipo de desporto condiciona o perfil de tarefa e o exercício da capitanía por parte dos líderes informais. Em futebol, menos da metade dos líderes informais são capitães (44.2%), destacando neles em maior medida a posição no campo que seu nível de jogo. Em basquete, uma grande parte dos líderes sim são capitães (77.1%) mas neles influi bem mais o nível de jogo que a ocupação de funções muito influentes no jogo colectivo. Os resultados relativizan as conclusoes de trabalhos previos sobre a influência do perfil de tarefa (Moran e Weiss, 2006)S

Electro-enzymatic ATP regeneration coupled to biocatalytic phosphorylation reactions

Funding Information: A.L.D, M.P. and M.V. thank grants RTI2018-095090-B-I00 and PID2021-1241160B-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by the European Union, and 2021AEP014 funded by CSIC. G.G.M. thanks grant BES-2016-078815 funded by MCIN/AEI/ 10.13039/501100011033 and by the European Union. I.L.-M. and M.V. acknowledge financial support through grant S2018/BAA-4403 SINOXPHOS-CM (EU-FEDER). I.A.C.P. and A.M.C. thank support from the Fundação para a Ciência e Tecnologia through fellowship SFRH/BD/146475/2019 and MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020, UIDP/04612/2020) and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Publisher Copyright: © 2023 The Author(s)Adenosine-5-triphosphate (ATP) is the main energy vector in biological systems, thus its regeneration is an important issue for the application of many enzymes of interest in biocatalysis and synthetic biology. We have developed an electroenzymatic ATP regeneration system consisting in a gold electrode modified with a floating phospholipid bilayer that allows coupling the catalytic activity of two membrane-bound enzymes: NiFeSe hydrogenase from Desulfovibrio vulgaris and F1Fo-ATP synthase from Escherichia coli. Thus, H2 is used as a fuel for producing ATP. This electro-enzymatic assembly is studied as ATP regeneration system of phosphorylation reactions catalysed by kinases, such as hexokinase and NAD+-kinase for respectively producing glucose-6-phosphate and NADP+.publishersversionpublishe

Efecto de una mezcla de galactooligosacáridos (GOS) / fructooligosacáridos (FOS) sobre la absorción intestinal y retención ósea de calcio, fósforo y magnesio: modelo experimental en ratas durante el crecimiento normal

Según las últimas encuestas nutricionales, la ingesta de calcio (Ca) está por debajo de las recomendaciones diarias internacionales. El aumento en la absorción y biodisponibilidad sería una estrategia ante la baja ingesta para prevenir efectos negativos sobre la masa ósea. Los galactooligosacáridos (GOS) y fructooligosacáridos (FOS) favorecen la absorción activa y pasiva de Ca. Objetivo: evaluar el efecto de GOS/FOS®, sobre la absorción y retención de Ca, Mg y P en un modelo experimental de ratas normales en crecimiento. Métodos: al destete, ratas machos Wistar recibieron: AIN93-G (A) o las siguientes fórmulas lácteas: Nutrilon (N), Vital (V) y Nutrilon Sin Lactosa (NSL) hasta los 40 días. N y NSL contienen 5,3% de GOS/FOS®, y V contiene los mismos nutrientes que N sin la mezcla prebiótica. Se evaluaron consumo de alimentos, absorción de Ca, Mg y P (mg/d y %) y desarrollo de lactobacilos; tras el sacrificio se midieron pH cecal, contenido mineral en fémur (CMF), biomecánica ósea y parámetros densitométricos (Lunar DXA). Resultados: el desarrollo de lactobacilos aumentó y el pH cecal disminuyó en los grupos N y NSL respecto de A y V (p<0,0001). La absorción de Ca, Mg y P aumentó significativamente (p<0,05) al igual que el CMF (p<0,05), los parámetros densitométricos (p<0,01) y biomecánicos (p<0,05). Conclusión: la mezcla GOS/FOS® utilizada favoreció la absorción de Ca, Mg y P, lo que indujo una mayor retención de mineral en hueso; esto asegura su efecto para suministrar una cantidad extra de Ca para favorecer la salud ósea.Fil: Bryk, Gabriel. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Zeni, Magali. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Medina, Daniel Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Pellegrini, Gretel Gisela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Pita Martín de Portela, María Luz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Zeni, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Bioquímica General y Bucal; Argentin