Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism

Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 Å resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses

Exact Solution of the Isovector Proton Neutron Pairing Hamiltonian

The complete exact solution of the T=1 neutron-proton pairing Hamiltonian is presented in the context of the SO(5) Richardson-Gaudin model with non-degenerate single-particle levels and including isospin-symmetry breaking terms. The power of the method is illustrated with a numerical calculation for $^{64}$Ge for a $pf+g_{9/2}$ model space which is out of reach of modern shell-model codes.Comment: To be published by Physical Review Letter

Atmospheric effects on remote sensing of non-uniform temperature sources

The equations of transfer, for a plane-parallel scattering atmosphere with a point source of energy on the lower bounding surface, were solved for various values of sensor/point source orientation and optical depths. Applications of this analysis to Skylab and ERTS mission are discussed, and requirements for atmospheric property data and radiation transfer properties are considered

U(3) and Pseudo-U(3) Symmetry of the Relativistic Harmonic Oscillator

We show that a Dirac Hamiltonian with equal scalar and vector harmonic oscillator potentials has not only a spin symmetry but an U(3) symmetry and that a Dirac Hamiltonian with scalar and vector harmonic oscillator potentials equal in magnitude but opposite in sign has not only a pseudospin symmetry but a pseudo-U(3) symmetry. We derive the generators of the symmetry for each case.Comment: 8 pages, 0 figures, pusblished in Physical Review Letters 95, 252501 (2005

Most Sub-Arcsecond Companions of Kepler Exoplanet Candidate Host Stars are Gravitationally Bound

Using the known detection limits for high-resolution imaging observations and the statistical properties of true binary and line-of-sight companions, we estimate the binary fraction of {\it Kepler} exoplanet host stars. Our speckle imaging programs at the WIYN 3.5-m and Gemini North 8.1-m telescopes have observed over 600 {\it Kepler} objects of interest (KOIs) and detected 49 stellar companions within $\sim$1 arcsecond. Assuming binary stars follow a log-normal period distribution for an effective temperature range of 3,000 to 10,000 K, then the model predicts that the vast majority of detected sub-arcsecond companions are long period ($P>50$ years), gravitationally bound companions. In comparing the model predictions to the number of real detections in both observational programs, we conclude that the overall binary fraction of host stars is similar to the 40-50\% rate observed for field stars

Understanding The Effects Of Stellar Multiplicity On The Derived Planet Radii From Transit Surveys: Implications for Kepler, K2, and TESS

We present a study on the effect of undetected stellar companions on the derived planetary radii for the Kepler Objects of Interest (KOIs). The current production of the KOI list assumes that the each KOI is a single star. Not accounting for stellar multiplicity statistically biases the planets towards smaller radii. The bias towards smaller radii depends on the properties of the companion stars and whether the planets orbit the primary or the companion stars. Defining a planetary radius correction factor $X_R$, we find that if the KOIs are assumed to be single, then, {\it on average}, the planetary radii may be underestimated by a factor of $\langle X_R \rangle \approx 1.5$. If typical radial velocity and high resolution imaging observations are performed and no companions are detected, this factor reduces to $\langle X_R \rangle \approx 1.2$. The correction factor $\langle X_R \rangle$ is dependent upon the primary star properties and ranges from $\langle X_R \rangle \approx 1.6$ for A and F stars to $\langle X_R \rangle \approx 1.2$ for K and M stars. For missions like K2 and TESS where the stars may be closer than the stars in the Kepler target sample, observational vetting (primary imaging) reduces the radius correction factor to $\langle X_R \rangle \approx 1.1$. Finally, we show that if the stellar multiplicity rates are not accounted for correctly, occurrence rate calculations for Earth-sized planets may overestimate the frequency of small planets by as much as $15-20$\%.Comment: 10 pages, 6 Figures, Accepted for publication in The Astrophysical Journal (Fix typo in Equation 6 of original astroph submission; correction also submitted to Journal

New Wrinkles on an Old Model: Correlation Between Liquid Drop Parameters and Curvature Term

The relationship between the volume and surface energy coefficients in the liquid drop A^{-1/3} expansion of nuclear masses is discussed. The volume and surface coefficients in the liquid drop expansion share the same physical origin and their physical connection is used to extend the expansion with a curvature term. A possible generalization of the Wigner term is also suggested. This connection between coefficients is used to fit the experimental nuclear masses. The excellent fit obtained with a smaller number of parameters validates the assumed physical connection.Comment: 6 pages, 2 figure

Prevalence of Anderson-Fabry disease in male patients with late onset hypertrophic cardiomyopathy

Background-Although studies have suggested that "late-onset" hypertrophic cardiomyopathy (HCM) may be caused by sarcomeric protein gene mutations, the cause of HCM in the majority of patients is unknown. This study determined the prevalence of a potentially treatable cause of hypertrophy, Anderson-Fabry disease, in a HCM referral population.Methods and Results-Plasma alpha-galactosidase A (alpha-Gal) was measured in 79 men with HCM who were diagnosed at greater than or equal to40 years of age (52.9 +/- 7.7 years; range, 40-71 years) and in 74 men who were diagnosed at <40 years (25.9 +/- 9.2 years; range, 8-39 years). Five patients (6.3%) with late-onset disease and 1 patient (1.4%) diagnosed at <40 years had low alpha-Gal activity. Of these 6 patients, 3 had angina, 4 were in New York Heart Association class 2, 5 had palpitations, and 2 had a history of syncope. Hypertrophy was concentric in 5 patients and asymmetric in 1 patient. One patient had left ventricular outflow tract obstruction. All patients with low alpha-Gal activity had alpha-Gal gene mutations.Conclusion-Anderson-Fabry disease should be considered in all cases of unexplained hypertrophy. Its recognition is important given the advent of specific replacement enzyme therapy

A mixed-mode shell-model theory for nuclear structure studies

We introduce a shell-model theory that combines traditional spherical states, which yield a diagonal representation of the usual single-particle interaction, with collective configurations that track deformations, and test the validity of this mixed-mode, oblique basis shell-model scheme on $^{24}$Mg. The correct binding energy (within 2% of the full-space result) as well as low-energy configurations that have greater than 90% overlap with full-space results are obtained in a space that spans less than 10% of the full space. The results suggest that a mixed-mode shell-model theory may be useful in situations where competing degrees of freedom dominate the dynamics and full-space calculations are not feasible.Comment: 20 pages, 8 figures, revtex 12p