The Threat of Plant Toxins and Bioterrorism: A Review

The intentional use of highly pathogenic microorganisms, such as bacteria, viruses or their toxins, to spread mass-scale diseases that destabilize populations (with motivations of religious or ideological belief, monetary implications, or political decisions) is defined as bioterrorism. Although the success of a bioterrorism attack is not very realistic due to technical constraints, it is not unlikely and the threat of such an attack is higher than ever before. It is now a fact that the capability to create panic has allured terrorists for the use of biological agents (BAs) to cause terror attacks. In the era of biotechnology and nanotechnology, accessibility in terms of price and availability has spread fast, with new sophisticated BAs often being produced and used. Moreover, there are some BAs that are becoming increasingly important, such as toxins produced by bacteria (e.g., Botulinum toxin, BTX), or Enterotoxyn type B, also known as Staphylococcal Enterotoxin B (SEB)) and extractions from plants. The most increasing records are with regards to the extraction / production of ricin, abrin, modeccin, viscumin and volkensin, which are the most lethal plant toxins known to humans, even in low amounts. Moreover, ricin was also developed as an aerosol biological warfare agent (BWA) by the US and its allies during World War II, but was never used. Nowadays, there are increasing records that show how easy it can be to extract plant toxins and transform them into biological weapon agents (BWAs), regardless of the scale of the group of individuals

Measurements of the SPS transverse impedance in 2000

We report on measurements of coherent tune shifts, head-tail growth rates, and current-dependent betatron phase advances at the CERN SPS in the year 2000. Comparing results obtained at two different energies shows that there is no notable contribution from space charge. Within the measurement resolution the impedance is the same as in 1999, consistent with the expected small effect from changes to ony a small number of pumping ports. In 2000, data were taken over an expanded range of chromaticities, which increases the sensitivity to the impedance frequency distribution. Measuremeents of the current-dependent phase advance around the ring help localizing the most important impedance sources

Experimental comparison in sensing breast cancer mutations by signal on and signal off paper-based electroanalytical strips

Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya.The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method

Nonlinear analysis of spacecraft thermal models

We study the differential equations of lumped-parameter models of spacecraft thermal control. Firstly, we consider a satellite model consisting of two isothermal parts (nodes): an outer part that absorbs heat from the environment as radiation of various types and radiates heat as a black-body, and an inner part that just dissipates heat at a constant rate. The resulting system of two nonlinear ordinary differential equations for the satellite's temperatures is analyzed with various methods, which prove that the temperatures approach a steady state if the heat input is constant, whereas they approach a limit cycle if it varies periodically. Secondly, we generalize those methods to study a many-node thermal model of a spacecraft: this model also has a stable steady state under constant heat inputs that becomes a limit cycle if the inputs vary periodically. Finally, we propose new numerical analyses of spacecraft thermal models based on our results, to complement the analyses normally carried out with commercial software packages.Comment: 29 pages, 4 figure

Electron Cloud: Observations with LHC-Type Beams in the SPS

In August 1999, strong pressure increases were observed in the SPS in the presence of the new LHC-type beams. This paper reports on observations of the electron cloud phenomenon and the related pressure increase as a function of parameters such as the number of protons per bunch, the number of bunches per batch, the shape of the vacuum chamber and the electron current collected on pick-ups. Results of the observed clean-up, "beam scrubbing" will be presented as well as the consequences of the e-cloud phenomenon on the SPS operation with the LHC nominal beam intensity

A new estimation of the recent tropospheric molecular hydrogen budget using atmospheric observations and variational inversion

This paper presents an analysis of the recent tropospheric molecular hydrogen (H2) budget with a particular focus on soil uptake and European surface emissions. A variational inversion scheme is combined with observations from the RAMCES and EUROHYDROS atmospheric networks, which include continuous measurements performed between mid-2006 and mid-2009. Net H2 surface flux, then deposition velocity and surface emissions and finally, deposition velocity, biomass burning, anthropogenic and N2 fixation-related emissions were simultaneously inverted in several scenarios. These scenarios have focused on the sensibility of the soil uptake value to different spatio-temporal distributions. The range of variations of these diverse inversion sets generate an estimate of the uncertainty for each term of the H2 budget. The net H2 flux per region (High Northern Hemisphere, Tropics and High Southern Hemisphere) varies between −8 and +8 Tg yr−1. The best inversion in terms of fit to the observations combines updated prior surface emissions and a soil deposition velocity map that is based on bottom-up and top-down estimations. Our estimate of global H2 soil uptake is −59±9 Tg yr−1. Forty per cent of this uptake is located in the High Northern Hemisphere and 55% is located in the Tropics. In terms of surface emissions, seasonality is mainly driven by biomass burning emissions. The inferred European anthropogenic emissions are consistent with independent H2 emissions estimated using a H2/CO mass ratio of 0.034 and CO emissions within the range of their respective uncertainties. Additional constraints, such as isotopic measurements would be needed to infer a more robust partition of H2 sources and sinks

Experimental Studies of Carbon Coatings as Possible Means of Suppressing Beam Induced Electron Multipacting in the CERN SPS

Electron cloud build-up is a major limitation for the operation of the SPS with LHC beam above nominal intensity. These beams are envisaged in the frame of the LHC luminosity upgrade and will be available from the new injectors LPSPL and PS2. A series of studies have been conducted in order to identify possible means to suppress electron multipacting by coating the existing SPS vacuum chambers with thin films of amorphous carbon. After a description of the experimental apparatus installed in the SPS, the results of the tests performed with beam in 2008 will be presented