310 research outputs found
Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms
We present a rapid and efficient method for the detection of related transcripts with different expression levels. This approach combines the rapid amplification of cDNA ends (RACE) method with a cDNA subtractive technique. The strategy is based on successive subtractions of prevalent isoforms resulting in enrichment of less expressed transcripts. For each subtraction, a biotinylated primer specific for the prevalent isoform is hybridized on the total cDNA and the hybrid is retained on a streptavidin affinity column. The unbound cDNA serves as a template for subsequent isoform identification. To illustrate its application we describe the isolation of three new actin cDNA isoforms in the freshwater planarian Dugesia (S) polychro
Structure and dynamics of Oxide Melts and Glasses : a view from multinuclear and high temperature NMR
Solid State Nuclear Magnetic Resonance (NMR) experiments allow characterizing
the local structure and dynamics of oxide glasses and melts. Thanks to the
development of new experiments, it now becomes possible to evidence not only
the details of the coordination state of the network formers of glasses but
also to characterize the nature of polyatomic molecular motifs extending over
several chemical bonds. We present results involving 31P homonuclear
experiments that allow description of groups of up to three phosphate units and
27Al/17O heteronuclear that allows evidencing μ3 oxygen bridges in
aluminate glasses and rediscussion of the structure of high temperature melts.Comment: Journal of Non-Crystalline Solids (2007) in press; Also available
online at: http://crmht.cnrs-orleans.fr/Intranet/Publications/?id=207
Characterizing groundwater flow and heat transport in fractured rock using Fiber-Optic Distributed Temperature Sensing
International audienceWe show how fully distributed space-time measurements with Fiber-Optic Distributed Temperature Sensing (FO-DTS) can be used to investigate groundwater flow and heat transport in fractured media. Heat injection experiments are combined with temperature measurements along fiber-optic cables installed in boreholes. Thermal dilution tests are shown to enable detection of cross-flowing fractures and quantification of the cross flow rate. A cross borehole thermal tracer test is then analyzed to identify fracture zones that are in hydraulic connection between boreholes and to estimate spatially distributed temperature breakthrough in each fracture zone. This provides a significant improvement compared to classical tracer tests, for which concentration data are usually integrated over the whole abstraction borehole. However, despite providing some complementary results, we find that the main contributive fracture for heat transport is different to that for a solute tracer
Metabolomic Characterization of Ovarian Epithelial Carcinomas by HRMAS-NMR Spectroscopy
Objectives. The objectives of the present study are to determine if a metabolomic study by HRMAS-NMR can (i) discriminate between different histological types of epithelial ovarian carcinomas and healthy ovarian tissue, (ii) generate statistical models capable of classifying borderline tumors and (iii) establish a potential relationship with patient's survival or response to chemotherapy. Methods. 36 human epithelial ovarian tumor biopsies and 3 healthy ovarian tissues were studied using 1H HRMAS NMR spectroscopy and multivariate statistical analysis. Results. The results presented in this study demonstrate that the three histological types of epithelial ovarian carcinomas present an effective metabolic pattern difference. Furthermore, a metabolic signature specific of serous (N-acetyl-aspartate) and mucinous (N-acetyl-lysine) carcinomas was found. The statistical models generated in this study are able to predict borderline tumors characterized by an intermediate metabolic pattern similar to the normal ovarian tissue. Finally and importantly, the statistical model of serous carcinomas provided good predictions of both patient's survival rates and the patient's response to chemotherapy. Conclusions. Despite the small number of samples used in this study, the results indicate that metabolomic analysis of intact tissues by HRMAS-NMR is a promising technique which might be applicable to the therapeutic management of patients
Nanostructured gadolinium-doped ceria microsphere synthesis from ion exchange resin: Multi-scale in-situ studies of solid solution formation
TOWARDS A DECISION SUPPORT TOOL FOR 3D VISUALISATION: APPLICATION TO SELECTIVITY PURPOSE OF SINGLE OBJECT IN A 3D CITY SCENE
Corresponding States of Structural Glass Formers
The variation with respect to temperature T of transport properties of 58
fragile structural glass forming liquids (68 data sets in total) are analyzed
and shown to exhibit a remarkable degree of universality. In particular,
super-Arrhenius behaviors of all super-cooled liquids appear to collapse to one
parabola for which there is no singular behavior at any finite temperature.
This behavior is bounded by an onset temperature To above which liquid
transport has a much weaker temperature dependence. A similar collapse is also
demonstrated, over the smaller available range, for existing numerical
simulation data.Comment: 6 pages, 2 figures. Updated References, Table Values, Submitted for
Publicatio
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