Effects of cytokinins on secondary somatic embryogenesis of selected clone Rayong 9 of Manihot esculenta Crantz for ethanol production

In 2005, the Rayong-FCRC released a new high-yielding cassava cultivar designated Rayong 9 (R9) for ethanol production. However, the rate of distribution to farmers has been limited by the traditional vegetative propagation method of this crop which does not always satisfy the needs in planting material. The objective was to improve secondary somatic embryogenesis of the cassava clone Rayong 9 (R9) selected in Thailand by the Rayong Field Crops Research Center (Rayong-FCRC) for its suitability to produce ethanol. Fragments of cotyledon-stage somatic embryos were subcultured onto MS medium supplemented with the auxin 2,4-dichlorophenoxyacetic acid (2,4-D) at 4 mg/l in sight of inducing secondary somatic embryogenesis. Five different cytokinins, 6-benzylaminopurine (BAP), kinetin, zeatin, isopentenyladenine (2-iP) and adenine were added at 1 mg/l to the induction and maturation media to test their efficiency. Onto cytokinin-free media, 70 to 80% of explants produced embryoids, each explant giving 2 to 4 new embryoids within 7 weeks. The conversion rate of the embryoids into plantlets ranged from 11 to 26% depending on the type of cytokinin. With the exception of adenine, the other cytokinins inhibited the intensity of somatic embryogenesis, by 75% in the case of zeatin and 30% in the case of kinetin. Addition of adenine did not significantly improve the number of embryoids per explant. However, at 10, 20 and 40 mg/l adenine tended to improve the process relatively to embryoid sizes and plantlet survival rates in the greenhouse.Key words: Adenine, biofuel, cassava, cotyledonary-stage, embryoids, icrocuttings, micropropagation

Global Analysis of Aerosol Properties Above Clouds

The seasonal and spatial varability of Aerosol Above Cloud (AAC) properties are derived from passive satellite data for the year 2008. A significant amount of aerosols are transported above liquid water clouds on the global scale. For particles in the fine mode (i.e., radius smaller than 0.3 m), including both clear sky and AAC retrievals increases the global mean aerosol optical thickness by 25(+/- 6%). The two main regions with man-made AAC are the tropical Southeast Atlantic, for biomass burning aerosols, and the North Pacific, mainly for pollutants. Man-made AAC are also detected over the Arctic during the spring. Mineral dust particles are detected above clouds within the so-called dust belt region (5-40 N). AAC may cause a warming effect and bias the retrieval of the cloud properties. This study will then help to better quantify the impacts of aerosols on clouds and climate

A pericentric inversion of chromosome 4 in pigs

International audienc

Remote sensing of aerosols by using polarized, directional and spectral measurements within the A-Train: the PARASOL mission

Instruments dedicated to aerosol monitoring are recently available and the POLDER (POLarization and Directionality of the Earth's Reflectances) instrument on board the PARASOL (Polarization & Anisotropy of Reflectances for Atmospheric Sciences coupled with Observations from a Lidar) mission is one of them. By measuring the spectral, angular and polarization properties of the radiance at the top of the atmosphere, in coordination with the other A-Train instruments, PARASOL provides the aerosol optical depths (AOD) as well as several optical and microphysical aerosol properties. The instrument, the inversion schemes and the list of aerosol parameters are described. Examples of retrieved aerosol parameters are provided as well as innovative approaches and further inversion techniques

Comprehensive tool for calculation of radiative fluxes: illustration of shortwave aerosol radiative effect sensitivities to the details in aerosol and underlying surface characteristics

The evaluation of aerosol radiative effect on broadband hemispherical solar flux is often performed using simplified spectral and directional scattering characteristics of atmospheric aerosol and underlying surface reflectance. In this study we present a rigorous yet fast computational tool that accurately accounts for detailed variability of both spectral and angular scattering properties of aerosol and surface reflectance in calculation of direct aerosol radiative effect. The tool is developed as part of the GRASP (Generalized Retrieval of Aerosol and Surface Properties) project. We use the tool to evaluate instantaneous and daily average radiative efficiencies (radiative effect per unit aerosol optical thickness) of several key atmospheric aerosol models over different surface types. We then examine the differences due to neglect of surface reflectance anisotropy, nonsphericity of aerosol particle shape and accounting only for aerosol angular scattering asymmetry instead of using full phase function. For example, it is shown that neglecting aerosol particle nonsphericity causes mainly overestimation of the aerosol cooling effect and that magnitude of this overestimate changes significantly as a function of solar zenith angle (SZA) if the asymmetry parameter is used instead of detailed phase function. It was also found that the nonspherical–spherical differences in the calculated aerosol radiative effect are not modified significantly if detailed BRDF (bidirectional reflectance distribution function) is used instead of Lambertian approximation of surface reflectance. Additionally, calculations show that usage of only angular scattering asymmetry, even for the case of spherical aerosols, modifies the dependence of instantaneous aerosol radiative effect on SZA. This effect can be canceled for daily average values, but only if sun reaches the zenith; otherwise a systematic bias remains. Since the daily average radiative effect is obtained by integration over a range of SZAs, the errors vary with latitude and season. In summary, the present analysis showed that use of simplified assumptions causes systematic biases, rather than random uncertainties, in calculation of both instantaneous and daily average aerosol radiative effect. Finally, we illustrate application of the rigorous aerosol radiative effect calculations performed as part of GRASP aerosol retrieval from real POLDER/PARASOL satellite observations

Aerosol retrieval experiments in the ESA Aerosol_cci project

Within the ESA Climate Change Initiative (CCI) project Aerosol_cci (2010–2013), algorithms for the production of long-term total column aerosol optical depth (AOD) datasets from European Earth Observation sensors are developed. Starting with eight existing pre-cursor algorithms three analysis steps are conducted to improve and qualify the algorithms: (1) a series of experiments applied to one month of global data to understand several major sensitivities to assumptions needed due to the ill-posed nature of the underlying inversion problem, (2) a round robin exercise of "best" versions of each of these algorithms (defined using the step 1 outcome) applied to four months of global data to identify mature algorithms, and (3) a comprehensive validation exercise applied to one complete year of global data produced by the algorithms selected as mature based on the round robin exercise. The algorithms tested included four using AATSR, three using MERIS and one using PARASOL. This paper summarizes the first step. Three experiments were conducted to assess the potential impact of major assumptions in the various aerosol retrieval algorithms. In the first experiment a common set of four aerosol components was used to provide all algorithms with the same assumptions. The second experiment introduced an aerosol property climatology, derived from a combination of model and sun photometer observations, as a priori information in the retrievals on the occurrence of the common aerosol components. The third experiment assessed the impact of using a common nadir cloud mask for AATSR and MERIS algorithms in order to characterize the sensitivity to remaining cloud contamination in the retrievals against the baseline dataset versions. The impact of the algorithm changes was assessed for one month (September 2008) of data: qualitatively by inspection of monthly mean AOD maps and quantitatively by comparing daily gridded satellite data against daily averaged AERONET sun photometer observations for the different versions of each algorithm globally (land and coastal) and for three regions with different aerosol regimes. The analysis allowed for an assessment of sensitivities of all algorithms, which helped define the best algorithm versions for the subsequent round robin exercise; all algorithms (except for MERIS) showed some, in parts significant, improvement. In particular, using common aerosol components and partly also a priori aerosol-type climatology is beneficial. On the other hand the use of an AATSR-based common cloud mask meant a clear improvement (though with significant reduction of coverage) for the MERIS standard product, but not for the algorithms using AATSR. It is noted that all these observations are mostly consistent for all five analyses (global land, global coastal, three regional), which can be understood well, since the set of aerosol components defined in Sect. 3.1 was explicitly designed to cover different global aerosol regimes (with low and high absorption fine mode, sea salt and dust)

Multi-species sociology of the body

The human body has become a central focus in sociology. Such work has centred largely on the human body and its significance in social contexts. This article draws on sociological understandings of human embodiment, especially the idea of the ‘body as a project’, to facilitate a multi-species understanding of bodies and their entanglements. Conceptualising the body as a project has provided sociological insights into the scientific and technological innovations that are designed to improve health and delay death. Nonhuman animals are entangled in these efforts, though their presence is often occluded. By examining notions of body masks, body regimes and body options, which are well established in sociological thinking about the body, this article seeks to prompt consideration of how to utilise theories of the body to examine human–nonhuman animal entanglements in order to establish a multi-species sociology of the body

Intercomparison of desert dust optical depth from satellite measurements

This work provides a comparison of satellite retrievalsof Saharan desert dust aerosol optical depth (AOD)during a strong dust event through March 2006. In this event,a large dust plume was transported over desert, vegetated,and ocean surfaces. The aim is to identify the differencesbetween current datasets. The satellite instruments consideredare AATSR, AIRS, MERIS, MISR, MODIS, OMI,POLDER, and SEVIRI. An interesting aspect is that the differentalgorithms make use of different instrument characteristicsto obtain retrievals over bright surfaces. These includemulti-angle approaches (MISR, AATSR), polarisationmeasurements (POLDER), single-view approaches using solarwavelengths (OMI, MODIS), and the thermal infraredspectral region (SEVIRI, AIRS). Differences between instruments,together with the comparison of different retrievalalgorithms applied to measurements from the same instrument,provide a unique insight into the performance andcharacteristics of the various techniques employed. As wellas the intercomparison between different satellite products,the AODs have also been compared to co-located AERONETdata. Despite the fact that the agreement between satellite andAERONET AODs is reasonably good for all of the datasets,there are significant differences between them when comparedto each other, especially over land. These differencesare partially due to differences in the algorithms, such as assumptionsabout aerosol model and surface properties. However,in this comparison of spatially and temporally averageddata, it is important to note that differences in sampling, relatedto the actual footprint of each instrument on the heterogeneousaerosol field, cloud identification and the qualitycontrol flags of each dataset can be an important issue