Genetic Variability in Ruditapes Decussatus Clam Combined With Perkinsus Infection Level to Support Founder Population Selection for a Breeding Program

[Abstract] Clam farmers worldwide face several challenges, including irregular seed supply and high mortalities due to pathogenic organisms such as "Perkinsus olseni". In Europe, there is a high unmet consumer demand for native clam species such as "Ruditapes decussatus". The high market value of "R. decussatus" makes the culture of this species potentially more attractive than that culture of the alien species "Ruditapes philippinarum". Thus, there is a market opportunity in breeding and producing "R. decussatus" at an industrial scale. A selective breeding program to improve "R. decussatus" performance will be carried out in Portugal; and the first critical step to develop such a breeding program is the establishment of a founder population. In this study, intra- and interpopulation genetic diversity was assessed using 13 microsatellite markers in eight natural beds located in Portugal, Spain and Italy. Also, allele and genotypic frequencies of each microsatellite locus were assessed discriminating between clams infected and non-infected by "P. olseni". All locations showed similar values for several genetic diversity parameters. Analyses of population differentiation (FST, Bayesian clustering and AMOVAs) revealed five genetically differentiated regions: Rías Altas and Rías Baixas (NW Spain), North/Central Coast of Portugal, Gulf of Cadiz and Adriatic Sea. Significant differences in the allelic and genotypic frequency distribution between infected clams and non-infected ones at four microsatellite loci are reported suggesting that resistance to the disease could have a genetic basis. Moreover, a positive or negative relationship between the frequency of certain alleles and the parasite infection was inferred. Further studies should confirm the potential use of those alleles as genetic markers for "P. olseni" infection. Integrating results of genetic diversity within and between populations and Perkinsus infection levels, a founder population for a "R. decussatu" breeding program is proposed, composed by individuals from Barallobre (Rías Altas), Pontevedra or Cangas (Rías Baixas), Óbidos (North/Central Coast of Portugal), Algarve (Gulf of Cadiz) and Venice (Adriatic Sea).Governo da República Portuguesa; 22390 (16/SI/2016) (CENTRO-01-0247-FEDER-022390)Ministério do Mar (Portugal); FA_01_2017_00

Quasi-synoptic transport, budgets and water mass transformation in the Azores–Gibraltar Strait region during summer 2009

We describe the circulation patterns in the Azores–Gibraltar Strait region (North-Eastern Atlantic) during the 2009 CAIBOX cruise on the basis of hydrographic and direct current velocity measurements. This study offers new data for a region where importation of central waters (subpolar and subtropical modes of Eastern North Atlantic Central Water) and exports of Mediterranean Water are strongly related to large-scale dynamics in the North Atlantic Ocean (Azores Current-Mediterranean Water system). The description is backed up quantitatively by the results of a box inverse model, which was used to obtain absolute water mass transport values consistent with thermal wind equations and with conservation of volume, salt and heat. The contributions of water masses were determined in an extended Optimum Multiparameter Analysis from a quasi-synoptic point of view, providing detail in addition to volume, salt and heat transport. The surface–subsurface large-scale current system in the region consists of the Azores Current (13.1 ± 2.5 Sverdrup [Sv], 1 Sv = 106 m3 s−1), the Azores Counter-Current (5.2 ± 2.1 Sv), the Portugal Current (4.5 ± 1.4 Sv) and the Canary Current (7.1 ± 1.1 Sv). Broadly speaking, central waters are imported into the CAIBOX region at a rate of 1.6 ± 0.9 Sv, and Mediterranean Water is exported at a rate of 1.5 ± 0.4 Sv. The downwelling of central waters west of Gibraltar Strait was quantified at 1.1 Sv. Not all this volume participates in MW formation, but 0.8 Sv of entrained central waters; of which 0.5 Sv are from central waters of subpolar origin and 0.3 Sv from subtropical central waters. Of the 4.9 Sv of subtropical central waters advected by the Azores Current, about 0.7 Sv would reach the Gulf of Cadiz region either to take part in central water entrainment or to flow across the Gibraltar Strait as part of the Atlantic inflow to the Mediterranean Sea

Surface waters of the NW Iberian margin: upwelling on the shelf versus outwelling of upwelled waters from the Rías Baixas

A set of hydrographic surveys were carried out in the Ría of Vigo (NW Spain) at 2–4 d intervals during four 2–3 week periods in 1997, covering contrasting seasons. Residual exchange fluxes with the adjacent shelf were estimated with a 2-D, non-steady-state, salinity–temperature weighted box model. Exchange fluxes consist of a steady-state term (dependent on the variability of continental runoff) and a non-steady-state term (dependent on the time changes of density gradients in the embayment). More than 95% of the short-time-scale variability of the exchange fluxes in the middle and outer ría can be explained by the non-steady-state term that, in turns, is correlated (R2>75%) with the offshore Ekman transport. Conversely, 96% of the variability of exchange fluxes in the inner ría rely on the steady-state term. The outer and middle ría are under the direct influence of coastal upwelling, which enhances the positive residual circulation pattern by an order of magnitude: from 10 2 to 10 3 m3s−1. On the contrary, downwelling provokes a reversal of the circulation in the outer ría. The position of the downwelling front along the embayment depends on the relative importance of Ekman transport (Qx, m3s−1km−1) and continental runoff (R, m3s−1). When Qx/ R>7±2 the reversal of the circulation affects the middle ría. Our results are representative for the ‘Rías Baixas’, four large coastal indentations in NW Spain. During the upwelling season (spring and summer), 60% of shelf surface waters off the ‘Rías Baixas’ consist of fresh Eastern North Atlantic Central Water (ENACW) upwelled in situ. The remaining 40% consists of upwelled ENACW that previously enters the rías and it is subsequently outwelled after thermohaline modification. During the downwelling season (autumn and winter), 40% of the warm and salty oceanic subtropic surface water, which piled on the shelf by the predominant southerly winds, enters the rias

Dating the Anthropocene in deep-sea sediments: a gamma spectrometric approach

1 poster presented at the International meeting of Sedimentology 2017 in Toulouse, France, from October 10th to 13thUtilizando muestras de sedimentos superficiales obtenidas durante la campaña OVIDE/BOCATS 2016 y una innovadora técnica para establecer cronologías absolutas, se ha obtenido una primera estimación cuantitativa de los flujos de carbono hacia sedimentos profundos en la cuenca subpolar de Irminger. La geocronología basada en espectrometría gamma de alta resolución y bajo fondo con dos detectores simultáneos de germanio hiper-puro (HPGe) es una técnica suficientemente precisa y sensible como para datar sedimentos pelágicos profundos. El papel cuantitativo del Irminger como sumidero de carbono durante el Antropoceno se evaluó combinando una cronología basada en el radionúclido natural 210Pb junto a análisis sedimentológicos y de composición elemental. La tasa media de sedimentación para el canal central del Irminger es de 0.83±0.14 mm·yr-1. Los cálculos de flujos de carbono concluyen que durante el Antropoceno 32±14 g·Cinorg·m-2·año-1 y 4.5±3 g·Corg·m-2·año-1 sedimentan en la cuenca del Irminger, suponiendo un considerable sumidero de carbono en el área cuantificado en más de 22 Tg-C·año−1N

Trends in anthropogenic CO2 in water masses of the Subtropical North Atlantic Ocean

12 páginas, 4 figuras,1 tabla.-- Proyecto CarbochangeThe variability in the storage of the oceanic anthropogenic CO2 (Cant) on decadal timescales is evaluated within the main water masses of the Subtropical North Atlantic along 24.5°N. Inorganic carbon measurements on five cruises of the A05 section are used to assess the changes in Cant between 1992 and 2011, using four methods (ΔC∗, TrOCA, φCT0, TTD). We find good agreement between the Cant distribution and storage obtained using chlorofluorocarbons and CO2 measurements in both the vertical and horizontal scales. Cant distribution shows higher concentrations and greater decadal storage rates in the upper layers with both values decreasing with depth. The greatest enrichment is obserbed in the central water masses, with their upper limb showing a mean annual accumulation of about 1 μmol kg−1 yr−1 and the lower limb showing, on average, half that value. We detect zonal gradients in the accumulation of Cant. This finding is less clear in the upper waters, where greater variability exists between methods. In accordance with data from time series stations, greater accumulation of Cant is observed in the upper waters of the western basin of the North Atlantic Subtropical Gyre. In intermediate and deep layers, the zonal gradient in the storage of Cant is more robust between methods. The much lower mean storage rates found along the section (<0.25 μmol kg−1 yr−1) become more obvious when longitudinal differences in the Cant accumulation are considered. In particular, west of 70°W the ventilation by the Labrador Sea Water creates a noticeable accumulation rate up to ∼0.5 μmol kg−1 yr−1 between 1000 and 2500 dbar. If a Transient Stationary State of the Cant distributions is considered, significant bi-decadal trends in the Cant storage rates in the deepest North Atlantic waters are detected, in agreement with recent estimations.We acknowledge funding from the Spanish Ministry of Economy and Competitiveness through Grants CSD2008-00077 (Circumnavigation Expedition MALASPINA 2010 Project), CTM2009-08849 (ACDC Project) and CTM2012-32017 (MANIFEST Project). We also acknowledge funding from the EU FP7 project CARBOCHANGE under Grant Agreement No. 264879 and by the Marine Biogeochemistry and Global Change research group (Generalitat de Catalunya, 2014SGR1029). E.F. Guallart was funded through a JAE-Pre grant that was financed by the Spanish National Research Council Agency (Consejo Superior de Investigaciones Científicas, CSIC) and by the European Social Fund.Peer reviewe

Air-sea CO2 fluxes in the Atlantic as measured during the FICARAM cruises

A total of fourteen hydrographic cruises spanning from 2000 to 2008 were conducted during the spring and autumn seasons between Spain and the Southern Ocean, under the framework of the Spanish research project FICARAM. The performed underway measurements are processed and analysed to describe the meridional air-sea CO2 fluxes (F CO2) along the Atlantic Ocean. The data was organised into different biogeochemical oceanographic provinces, according mainly to the thermohaline characteristics. The obtained spatial and temporal distributions of F CO2 follow the generally expected patterns and annual trends. The Subtropical regions in both hemispheres alternated the CO2 source and sink nature from autumn to spring, respectively. On the other hand, Tropical waters and the Patagonian Sea clearly behaved as sinks of atmospheric CO2 like the waters of the Drake Passage during autumn. The obtained results during the cruises also revealed significant long-term trends, such as the warming of equatorial waters (0.11±0.03 Cyr−1) and the decrease of surface salinity (−0.16±0.01 yr−1) in tropical waters caused by the influence of the Amazon River plume. This reduction in surface salinity appears to have a direct influence over the CO2 storage rates, fostering the uptake capacity of atmospheric CO2 (−0.09±0.03 molm−2 yr−1). An analysis of the biogeochemical forcing on the CO2 fugacity (fCO2) variability performed from an empirical algorithm highlighted the major role of the Amazon River input in the tropical North Atlantic fluxes. In addition, it has provided a quantitative measure of the importance of the thermodynamic control of F CO2 at temperate latitudes

Échanges air—océan de CO2 dans une baie côtière affectée par l'upwelling : contrôle physique et biologique

17 páginas, 4 tablas, 8 figuras[EN] Water column pCO2 and air-sea CO2 fluxes were studied during an 18-month period (May 1994-September 1995)in a coastal embayment affected by upwelling, located in the northwestern Iberian Peninsula (Ria de Vigo and adjacent shelf). Overall, the region acted as a net annual atmospheric CO2 sink, with magnitude ranging from 0.54 mgC mm2 d-’ in the Ria estuary to 22 mgC mm2 d-’ offshore. During moderate upwelling and upwelling relaxation conditions the sampling area was a sink for atmospheric CO2. By contrast, during winter conditions and during intense upwelling the flux reversed towards the atmosphere. The relative influence of physical and biological processes on pC10, was evaluated using two different approaches: firstly, statistical analysis of physico-chemical correlations, and secondly, a thermodynamic analysis in the oceanic CO2 system. Both methods yielded consistent results, showing that the main processes controlling seasonal and spatial pC02 variability were the production and remineralization of organic matter, explaining ca. 70 % of the total variability. In the inner part of the embayment, air-sea CO2 exchange was mainly modulated by CO2 partial pressure gradient, whereas in the adjacent shelf, wind speed largely contributed to CO2 fluxes between the ocean and the atmosphere.[FR] Le pCO2 dnas le colonne d'eau et les échanges de CO2 entre l'air et l'eau ont été étudiés durant 18 mois (mai 1994-septembre 1995) dans une baie affectee par l’upwelling au nord-ouest de la peninsule Iberique (Ria de Vigo et plateau continental adjacent). En moyenne annuelle, cette region est un puits de CO2 atmosphtrique, le flux net air-mer variant de 0,54 a 22 mgC mm2j- l. Durant les periodes d’upwelling mod&t ou de relaxation de l’upwelling, la zone CtudiCe se comporte comme un puits de CO2 ; en revanche, durant l’hiver et lors d’evenements advectifs forts, cette zone devient une source de CO2 pour l’atmosphere. L’influence relative des processus physiques et biologiques a CtC Cvaluee par deux approches differenms : une analyse statistique et une analyse physico-chimique a partir des relations thermodynamiques. Ces deux methodes conduisent a des resultats comparables et montrent que les processus principaux qui controlent la variabilite spatiale et saisonniere du pC02 sont la production et la remineralisation de la mat&e organique, qui expliquent 70 % de la variabilite totale. A l’indrieur de la baie, les Cchanges air-mer de CO2 sont principalement modules par le gradient de pression partielle de CO2, tandis que sur le plateau continental, ils le sont par le coefficient d’echange.Comisión Interministerial de Ciencia y Tecnología, project AMB92-0165Peer reviewe

High spatial resolution alkalinity and pH measurements by IIm-CSIC group along 24.5ºN durinf the R/V Hespérides WOCE Section A05 cruise (July 14-August 15, 1992)

23 páginas, 8 figuras, 3 tablasPeer reviewe