Surgery-Induced Changes in Erythrocyte and Plasma Lipid Peroxidation, Enzymatic and Non-Enzymatic Antioxidants of Female Rats: Protective Role of Heparin and Pentoxifylline

Background: Intra abdominal adhesions are a complication that occurs after many abdominal surgical procedures, especially gynecological operations. These complications occur by irritating the peritoneum due to such circumstances as infection or surgical trauma, and are considered a pathological part of the healing process of peritoneal injury. It manifests itself with symptoms such as pain, intestinal or urethral obstruction and abdominal abscesses. Oxidative stress due to adhesions plays an important role on adhesion formation. In addition to many researches done at the point of prevention of adhesion and decreasing stress parameters, in this study, it was planned to determine the effect of Heparin (H) and Pentoxifylline (PTX) on malondialdehyde (MDA) and some antioxidant values.Materials, Methods & Results: This study was performed on rats and thirty-seven female Sprague-Dawley rats were randomized into four groups. The first group was sham (Sh) group (n = 7) and laparotomy was performed and 2 mL of 0.9 % NaCl was applied. For all other rats (n = 30) the small intestine was withdrawn and the uterus was uncovered and the anti mesenteric surfaces of the left uterine horn and left abdominal wall were superficially tilted until slight bleeding was seen. Lesion areas have been covered. Two mL 0.9 % NaCl to control (C) group (n = 10), 500 IU heparin to group H, and 25 mg / kg Pentoxifylline to group PTX (n = 10) have been given and then the abdominal incision was closed. The adhesion score of group Sh was found to be more important than C and PTX groups (P < 0.05). The adhesion score of group C was determined to be more significant than group H (P < 0.05). In group Sh, erythrocyte reduced glutathione (GSH) levels were found to be more significant (P < 0.01) than C, H and PTX groups whereas it was found that group C was more significant than group H (P < 0.01). In group Sh, glutathione peroxidase (GSH-Px) activity was found to be more significant (P < 0.05) than H and PTX groups, while MDA level was found to be lower and more significant (P < 0.01) than group C. In addition, group C was found to be higher and more significant (P < 0.01) than group H. Plasma vitamin E levels in Sh, H and PTX groups were found to be higher and more significant (P < 0.01) than group C. It is also observed that MDA levels in group C are higher and more significant (P < 0.01) than all groups. Nitric oxide (NO) level in Sh group was found to be lower and more significant (P < 0.05) than the other groups whereas it is determined that NO level in H group is higher and more significant (P < 0.05) than group C. Activites of erythrocyte catalase (CAT) and superoxide dismutase (SOD), the levels of plasma vitamin A and β-carotene were not important in all groups.Discussion: Intra abdominal adhesions that occur due to operations performed in the abdominal region still remain as a major problem. This adhesion brings certain problems with itself. Various applications have been made to reduce these problems. In this study, it was observed that inhibition of heparin and pentoxifylline adhesions contributed to the reduction of MDA levels and the reduction of oxidative damage due to the increase in levels of Vitamin E, GSH, GSH-Px and NO levels. In conclusion, it is suggested that heparin and pentoxifylline should be used to prevent adhesions due to abdominal operations and to reduce oxidative damage

5-(4-methoxybenzoyl)-4-(4-methoxyphenyl)-1-[1-(4-methoxyphenyl)ethylidenenamino]pyrimidin-2(1H)-one

In the title compound, C27H23N3O5, the pyrimidine ring is slightly puckered. The mean plane of the pyrimidine ring forms dihedral angles of 28.26 (10), 55.53 (9) and 55.96 (10)degrees with the three benzene rings in the molecule. There are intramolecular and intermolecular C-H...O interactions

‘Amara – Introduction

‘Amara est le dernier faubourg nord de la ville dans la continuité des faubourgs de Souk Sarouja et de ‘Uqayba. Ses limites sont à l’ouest le mur du cimetière Dahdah, au nord le boulevard de Bagdad, au sud l’axe de Souk Sarouja et l’avenue Malik Fayçal et à l’est, en 1930, les jardins de l’oasis. À la même époque, le boulevard de Bagdad qui a été tracé quelques années auparavant n’est toujours pas urbanisé, il joue plutôt le rôle de voie périphérique isolant la ville de l’oasis. Rue Jawdat An..

In vivo nephroprotective efficacy of propolis against contrast-induced nephropathy

PURPOS

Correlation of elevated leptin levels in amniotic fluid and maternal serum in neural tube defects

OBJECTIVE: To measure maternal serum and amniotic fluid leptin concentrations in pregnant women diagnosed antenatally as having fetuses with a neural tube defect in the second trimester

Yumurtacı japon bıldırcınlarında diyete ilave edilen zeytin yaprağı özütü ve zarlı yumurta kabuğunun performans, yumurta kalitesi, kan biyokimyasal ve kemik parametreleri üzerine etkileri

This study was conducted to determine the effects of dietary supplementation of olive leaf extract (OLE), eggshell with the membrane (ESM), and the ESM that absorbed the OLE (OLE+ESM) on the performance, egg quality, biochemical, and bone parameters in laying Japanese quail. A total of 112 quail, being 45-day-old, were divided into 4 groups with 4 replicates. The quail were fed with four diets: i) basal diet ii) basal diet supplemented with 400 ppm OLE iii) basal diet supplemented with 2% ESM, and iv) basal diet supplemented with 2% ESM that absorbed with 400 ppm OLE. Egg weight was observed to be higher in the OLE group (P0.05). There was no statistical difference in tibia bone parameters (P>0.05). The lowest concentration of serum lactate dehydrogenase (LDH) was observed in control group (P<0.01). Serum uric acid level decreased in ESM group (P<0.01). OLE supplementation had limited impacts on quail nutrition. Consequently, while the individual usage of OLE and ESM did not show remarkable effects, the mixture of OLE and ESM has been found to positively affect the egg quality and performance parameters

Synthesis and biological evaluation of new 3(2H)-pyridazinone derivatives as non-toxic anti-proliferative compounds against human colon carcinoma HCT116 cells

Novel 3(2H)-pyridazinone derivatives were designed, synthesised in satisfactory yields and evaluated in different experimental assays to assess their preliminary toxicity in vivo and anti-proliferative effects against HCT116 cell lines in vitro. Artemia salina lethality test provided LC50 values >100 mu g/mL for all compounds. Successive assays revealed that some compounds were endowed with a promising anti-proliferative effect against HCT116 cells, alone or stimulated by serotonin as a pro-inflammatory factor in order to mimick an inflamed model in vivo of cancer cell microenvironment. Moreover, the kinurenic acid level after treatment with these newly synthesised compounds was monitored as a marker of anti-proliferation in colon carcinoma models. The IC50 values obtained for the best-in-class compounds were comparable to that of daunorubicin as a reference drug. Conversely, these compounds were not able to counteract the spontaneous migration of human cancer HCT116 cell line in the wound healing paradigm