318 research outputs found
Fluorescent protein-mediated colour polymorphism in reef corals: multicopy genes extend the adaptation/acclimatization potential to variable light environments
The genomic framework that enables corals to adjust to unfavourable conditions is crucial for coral reef survival in a rapidly changing climate. We have explored the striking intraspecific variability in the expression of coral pigments from the green fluorescent protein (GFP) family to elucidate the genomic basis for the plasticity of stress responses among reef corals. We show that multicopy genes can greatly increase the dynamic range over which corals can modulate transcript levels in response to the light environment. Using the red fluorescent protein amilFP597 in the coral Acropora millepora as a model, we demonstrate that its expression increases with light intensity, but both the minimal and maximal gene transcript levels vary markedly among colour morphs. The pigment concentration in the tissue of different morphs is strongly correlated with the number of gene copies with a particular promoter type. These findings indicate that colour polymorphism in reef corals can be caused by the environmentally regulated expression of multicopy genes. High-level expression of amilFP597 is correlated with reduced photodamage of zooxanthellae under acute light stress, supporting a photoprotective function of this pigment. The cluster of light-regulated pigment genes can enable corals to invest either in expensive high-level pigmentation, offering benefits under light stress, or to rely on low tissue pigment concentrations and use the conserved resources for other purposes, which is preferable in less light-exposed environments. The genomic framework described here allows corals to pursue different strategies to succeed in habitats with highly variable light stress levels. In summary, our results suggest that the intraspecific plasticity of reef corals’ stress responses is larger than previously thought
Leistungstest, Offenheit von Bildungsgängen und obligatorische Beratung der Eltern: Reduziert das Deutschfreiburger Übergangsmodell die Effekte des sozialen Hintergrunds bei Übergangsentscheidungen?
Dieser Beitrag untersucht das Übertrittsverfahren von der Grundschule in die Sekundarschule I der deutschsprachigen Schulen des Kantons Freiburg (Schweiz). Das Deutschfreiburger Übergangsmodell mit seinen verschiedenen Komponenten wird vorgestellt und evaluiert. Ein besonderes Augenmerk liegt dabei auf der Frage, ob unerwünschte Effekte des familiären Hintergrunds durch dieses Modell reduziert bzw. eliminiert werden können. Untersucht wird im Weiteren die Übereinstimmung der Übertrittsempfehlung von Eltern und Lehrkräften sowie der Prüfungsleistung. Die Autoren kommen zum Schluss, dass das untersuchte Übergangsmodell die Effekte des familiären Hintergrunds beim Übertritt von der Primarschule in die Sekundarschule relativ gering hält. Der sozioökonomische Hintergrund wirkt sich über die Übertrittsempfehlung von Lehrkräften und Eltern auch auf den tatsächlichen Übertritt aus; die absoluten Effekte des familiären Hintergrunds fallen jedoch – nach Kontrolle der Schulleistung – insgesamt vergleichsweise schwach aus. Der eingesetzte Bewertungsbogen, den Lehrkräfte und Eltern zusätzlich zu den Noten als Basis fürm die Übergangsempfehlung einsetzen, scheint resistent gegenüber Effekten des familiären Hintergrunds zu sein
Positive selection at codon 38 of the human KCNE1 (= minK) gene and sporadic absence of 38Ser-coding mRNAs in Gly38Ser heterozygotes
<p>Abstract</p> <p>Background</p> <p>KCNE1 represents the regulatory beta-subunit of the slowly activating delayed rectifier potassium channel (IKs). Variants of KCNE1 have repeatedly been linked to the long-QT syndrome (LQTS), a disorder which predisposes to deafness, ventricular tachyarrhythmia, syncope, and sudden cardiac death.</p> <p>Results</p> <p>We here analyze the evolution of the common Gly38Ser variant (rs1805127), using genomic DNAs, complementary DNAs, and HEK293-expressed variants of altogether 19 mammalian species. The between species comparison reveals that the human-specific Gly38Ser polymorphism evolved under strong positive Darwinian selection, probably in adaptation to specific challenges in the fine-tuning of IKs channels. The involved amino acid exchanges (Asp > Gly, Gly > Ser) are moderately radical and do not induce apparent changes in posttranslational modification. According to population genetic analyses (HapMap phase II) a heterozygote advantage accounts for the maintenance of the Gly38Ser polymorphism in humans. On the other hand, the expression of the 38Ser allele seems to be disadvantageous under certain conditions, as suggested by the sporadic deficiency of 38Ser-coding mRNAs in heterozygote Central Europeans and the depletion of homozygotes 38Ser in the Yoruban sample.</p> <p>Conclusion</p> <p>We speculate that individual differences in genomic imprinting or genomic recoding might have contributed to conflicting results of recent association studies between Gly38Ser polymorphism and QT phenotype. The findings thus highlight the relevance of mRNA data in future association studies of genotypes and clinical disorders. To the best of our knowledge, they moreover provide first time evidence for a unique pattern; i.e. coincidence of positive Darwinian selection and polymorphism with a sporadically suppressed expression of one allele.</p
JOKARUS - Design of a compact optical iodine frequency reference for a sounding rocket mission
We present the design of a compact absolute optical frequency reference for
space applications based on hyperfine transitions in molecular iodine with a
targeted fractional frequency instability of better than . It
is based on a micro-integrated extended cavity diode laser with integrated
optical amplifier, fiber pigtailed second harmonic generation wave-guide
modules, and a quasi-monolithic spectroscopy setup with operating electronics.
The instrument described here is scheduled for launch end of 2017 aboard the
TEXUS 54 sounding rocket as an important qualification step towards space
application of iodine frequency references and related technologies. The
payload will operate autonomously and its optical frequency will be compared to
an optical frequency comb during its space flight
A profile shape correction to reduce the vertical sensitivity of cosmic-ray neutron sensing of soil moisture
n recent years, cosmic-ray neutron sensing (CRNS) has shown a large potential among proximal sensing techniques to monitor soil moisture noninvasively, with high frequency and a large support volume (radius up to 240 m and sensing depth up to 80 cm). This signal is, however, more sensitive to closer distances and shallower depths. Inherently, CRNS-derived soil moisture is a spatially weighted value, different from an average soil moisture as retrieved by a sensor network. In this study, we systematically test a new profile shape correction on CRNS-derived soil moisture, based on additional soil moisture profile measurements and vertical unweighting, which is especially relevant during pronounced wetting or drying fronts. The analyses are conducted with data collected at four contrasting field sites, each equipped with a CRNS probe and a distributed soil moisture sensor network. After applying the profile shape correction on CRNS-derived soil moisture, it is compared with the sensor network average. Results show that the influence of the vertical sensitivity of CRNS on integral soil moisture values is successfully reduced. One to three properly located profile measurements within the CRNS support volume improve the performance. For the four investigated field sites, the RMSE decreased 11–53% when only one profile location was considered. We therefore recommend to install along with a CRNS at least one soil moisture profile in a radial distanceProfile-shape-corrected, CRNS-derived soil moisture is an unweighted integral soil moisture over the support volume, which is easier to interpret and easier to use for further applications
RITA modulates cell migration and invasion by affecting focal adhesion dynamics
RITA, the RBP-J interacting and tubulin-associated protein, has been reported to be related to tumor development, but the underlying mechanisms are not understood. Since RITA interacts with tubulin and coats microtubules of the cytoskeleton, we hypothesized that it is involved in cell motility. We show here that depletion of RITA reduces cell migration and invasion of diverse cancer cell lines and mouse embryonic fibroblasts. Cells depleted of RITA display stable focal adhesions (FA) with elevated active integrin, phosphorylated focal adhesion kinase, and paxillin. This is accompanied by enlarged size and disturbed turnover of FA. These cells also demonstrate increased polymerized tubulin. Interestingly, RITA is precipitated with the lipoma-preferred partner (LPP), which is critical in actin cytoskeleton remodeling and cell migration. Suppression of RITA results in reduced LPP and alpha-actinin at FA leading to compromised focal adhesion turnover and actin dynamics. This study identifies RITA as a novel crucial player in cell migration and invasion by affecting the turnover of FA through its interference with the dynamics of actin filaments and microtubules. Its deregulation may contribute to malignant progression
IL-20 subfamily cytokines impair the oesophageal epithelial barrier by diminishing filaggrin in eosinophilic oesophagitis.
OBJECTIVE
Disruption of the epithelial barrier plays an essential role in developing eosinophilic oesophagitis (EoE), a disease defined by type 2 helper T cell (Th2)-mediated food-associated and aeroallergen-associated chronic inflammation. Although an increased expression of interleukin (IL)-20 subfamily members, IL-19, IL-20 and IL-24, in Th2-mediated diseases has been reported, their function in EoE remains unknown.
DESIGN
Combining transcriptomic, proteomic and functional analyses, we studied the importance of the IL-20 subfamily for EoE using patient-derived oesophageal three-dimensional models and an EoE mouse model.
RESULTS
Patients with active EoE have increased expression of IL-20 subfamily cytokines in the oesophagus and serum. In patient-derived oesophageal organoids stimulated with IL-20 cytokines, RNA sequencing and mass spectrometry revealed a downregulation of genes and proteins forming the cornified envelope, including filaggrins. On the contrary, abrogation of IL-20 subfamily signalling in Il20R2 -/- animals resulted in attenuated experimental EoE reflected by reduced eosinophil infiltration, lower Th2 cytokine expression and preserved expression of filaggrins in the oesophagus. Mechanistically, these observations were mediated by the mitogen-activated protein kinase (MAPK); extracellular-signal regulated kinases (ERK)1/2) pathway. Its blockade prevented epithelial barrier impairment in patient-derived air-liquid interface cultures stimulated with IL-20 cytokines and attenuated experimental EoE in mice.
CONCLUSION
Our findings reveal a previously unknown regulatory role of the IL-20 subfamily for oesophageal barrier function in the context of EoE. We propose that aberrant IL-20 subfamily signalling disturbs the oesophageal epithelial barrier integrity and promotes EoE development. Our study suggests that specific targeting of the IL-20 subfamily signalling pathway may present a novel strategy for the treatment of EoE
Characterization of IL-19, -20, and -24 in acute and chronic kidney diseases reveals a pro-fibrotic role of IL-24
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